[go: up one dir, main page]
More Web Proxy on the site http://driver.im/ Skip to main content
Springer Nature Link
Log in

Hormonal regulation of steroidogenic acute regulatory (StAR) protein messenger ribonucleic acid expression in the rat ovary

  • Published:
Endocrine Aims and scope Submit manuscript

Abstract

Steroidogenic acute regulatory (StAR) protein is thought to mediate the rapid increase in steroid hormone biosynthesis in response to tropic hormones by facilitating cholesterol transport to the inner mitochondrial membrane where the P450 side-chain cleavage enzyme (P450scc) is located. Since cholesterol delivery is the regulated step in steroidogenesis and is dependent onde novo protein synthesis, StAR mRNA levels were examined in response to the tropic hormones, pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG). The results of this investigation revealed that major StAR mRNA transcripts of 3.4 and 1.6 kb and a less abundant transcript of 1.2 kb were detected in the adrenal, ovary, and testis. Within the ovary, StAR mRNA levels were regulated by PMSG and hCG. The two major transcripts were increased in the immature rat ovary following PMSG administration and in the ovary, 8 d after ovulation, in response to stimulation by hCG. Serum progesterone levels were increased following hCG treatment in parallel with the enhanced expression of StAR. Following PMSG treatment, ovarian StAR transcripts at 3.4 and 1.6 kb were each increased twofold. In the ovary, 8 d following ovulation, basal ovarian StAR mRNA levels were elevated up to sixfold relative to the preovulatory StAR mRNA levels. Even with the enhanced basal level of StAR mRNA within the ovary 8 d postovulation, hCG administration still resulted in a 2.5- and 7-fold increase in the 3.4 and 1.6 kb (p<0.025) transcripts, respectively, and a 58% increase in serum progesterone. In contrast to the dramatic alterations in StAR mRNA expression following hormonal stimulation, P450scc mRNA levels remained unchanged in response to hCG stimulation. The levels of serum progesterone paralleled the change in ovarian StAR mRNA in all experiments. This study provides the first evidence that StAR mRNA expression in the rat ovary is mediated by gonadotropins, further supporting its important role in the regulation of steroid hormone biosynthesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
£29.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (United Kingdom)

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Alberta, J. A., Epstein, L. F., Pon, L. A., and Orme-Johnson, N. R. (1989).J. Biol. Chem. 264, 2368–2372.

    PubMed  CAS  Google Scholar 

  • Chomczynski, P. and Sacchi, N. (1987).Anal. Biochem. 162, 156–159.

    Article  PubMed  CAS  Google Scholar 

  • Clark, B. J., Wells, J., King, S. R., and Stocco, D. M. (1994).J. Biol. Chem. 269, 28,314–28,322.

    CAS  Google Scholar 

  • Clark, B. J., Soo, S. C., Caron, K. M., Ikeda, Y., Parker, K. L., and Stocco, D. M. (1995).Molec. Endocrinol. 9, 1345–1355.

    Article  Google Scholar 

  • Cooke, B. A., Janszen, F. H. A., Clostscher, W. F., and van der Molen, H. J. (1975).Biochem. J. 150, 413–418.

    PubMed  CAS  Google Scholar 

  • Crivello, J. C. F. and Jefcoate, C. R. (1980).J. Biol. Chem. 255, 8144–8151.

    PubMed  CAS  Google Scholar 

  • Epstein, L. F. and Orme-Johnson, N. R. (1991a).J. Biol. Chem. 266, 19,739–19,745.

    CAS  Google Scholar 

  • Epstein, L. F. and Orme-Johnson, N. R. (1991b).Molec. Cell. Endocrinol. 81, 113–126.

    Article  PubMed  CAS  Google Scholar 

  • Epstein, L. F., Alberta, J. A., Pon, L. A., and Orme-Johnson, N. R. (1989).Endocr. Res. 15, 117–127.

    Article  PubMed  CAS  Google Scholar 

  • Garren, L. D., Ney, R. L., and Davis, W. W. (1965).Proc. Natl. Acad. Sci. USA 53, 1443–1450.

    Article  PubMed  CAS  Google Scholar 

  • Gibori, G., Antczak, E., and Rothchild, I. (1977).Endocrinology 100, 1483–1495.

    PubMed  CAS  Google Scholar 

  • Goldring, N. B., Durica, J. M., Lifka, J., Hedin, L., Ratoosh, S. L., Miller, W. L., Orly, J., and Richards, J. S. (1987).Endocrinology 120, 1942–1950.

    PubMed  CAS  Google Scholar 

  • Griffin, G. E. and Orme-Johnson, N. R. (1991).J. Steroid Biochem. Molec. Biol. 40, 421–429.

    Article  Google Scholar 

  • Hartung, S., Rust, W., Balvers, M., and Ivell, R. (1995).Biochem. Biophys. Res. Comm.,215, 646–653.

    Article  PubMed  CAS  Google Scholar 

  • Hendrick, J. P., Hodges, P. E., and Rosenberg, L. E. (1989).Proc. Natl. Acad. Sci. USA 86, 4056–4060.

    Article  PubMed  CAS  Google Scholar 

  • Iida, S., Papadopoulos, V., and Hall, P. F. (1989).Endocrinology 124, 2619–2624.

    PubMed  CAS  Google Scholar 

  • Jefcoate, C. R., DiBartolomeos, M. J., Williams, C. A., and McNamara, B. C. (1987).J. Steroid Biochem. 27, 721–729.

    Article  PubMed  CAS  Google Scholar 

  • King, S. R., Ronen-Fuhrmann, T., Timberg, R., Clark, B. J., Orly, J., and Stocco, D. M. (1995).Endocrinology 136, 5165–5176.

    Article  PubMed  CAS  Google Scholar 

  • Krueger, R. J. and Orme-Johnson, N. R. (1988).Endocrinology 122, 1869–1875.

    PubMed  CAS  Google Scholar 

  • Lambeth, J. D., Xu, X. X., and Glover, M. (1987).J. Biol. Chem. 262, 9181–9188.

    PubMed  CAS  Google Scholar 

  • Lin, D., Sugawara, T., Strauss III, J. F., Clark, B. J., Stocco, D. M., Saenger, P., Rogol, A., and Miller, W. L. (1995).Science 267, 1828–1831.

    Article  PubMed  CAS  Google Scholar 

  • McLean, M. P., Puryear, T. K., Khan, I., Azhar, S., Billheimer, J. T., and Gibori, G. (1989).Endocrinology 125, 1337–1344.

    PubMed  CAS  Google Scholar 

  • McLean, M. P., Billheimer, J. T., Warden, K. J., and Irby, R. B. (1995).Endocrinology 136, 3360–3368.

    Article  PubMed  CAS  Google Scholar 

  • Mendelson, C., Dufau, M., and Catt, K. (1975).Biochim. Biophys. Acta 411, 222–230.

    PubMed  CAS  Google Scholar 

  • Pon, L. A. and Orme-Johnson, N. R. (1986).J. Biol. Chem. 261, 6594–6599.

    PubMed  CAS  Google Scholar 

  • Pon, L. A. and Orme-Johnson, N. R. (1988).Endocrinology 123, 1942–1948.

    Article  PubMed  CAS  Google Scholar 

  • Pon, L. A., Hartigan, J. A., and Orme-Johnson, N. R. (1986a).J. Biol. Chem. 261, 13,309–13,316.

    CAS  Google Scholar 

  • Pon, L. A., Epstein, L. F., and Orme-Johnson, N. R. (1986b).Endocr. Res. 12, 429–446.

    PubMed  CAS  Google Scholar 

  • Privalle, C. T., Crivello, J. F., and Jefcoate, C. R. (1983a).Proc. Natl. Acad. Sci. USA 80, 702–706.

    Article  PubMed  CAS  Google Scholar 

  • Privalle, C. T., McNamara, B. C., Dhariwal, M. S., and Jefcoate, C. R. (1983b).Mol. Cell. Endocrinol. 53, 87–101.

    Article  Google Scholar 

  • Rigby, P. W., Dieckmann, M., Rhodes, C., and Berg, P. (1977).J. Mol. Biol. 113, 237–251.

    Article  PubMed  CAS  Google Scholar 

  • Sanger, F., Niklen, S., and Coulson, A. R. (1977).Proc. Natl. Acad. Sci. USA 74, 5463–5467.

    Article  PubMed  CAS  Google Scholar 

  • Sugawara, T., Holt, J. A., Driscoll, D., Strauss III, J. F., Lin, D., Miller, W. L., Patterson, D., Clancy, K. P., Hart, I. M., Clark, B. J., and Stocco, D. M. (1995).Proc. Natl. Acad. Sci. USA 92, 4778–4782.

    Article  PubMed  CAS  Google Scholar 

  • Stocco, D. M. (1992).J. Steroid Biochem. Molec. Biol. 43, 319–333.

    Article  PubMed  CAS  Google Scholar 

  • Stocco, D. M. and Ascoli, M. (1993).Endocrinology 132, 959–967.

    Article  PubMed  CAS  Google Scholar 

  • Stocco, D. M. and Chen, W. (1991).Endocrinology 128, 1918–1926.

    PubMed  CAS  Google Scholar 

  • Stocco, D. M. and Clark, B. J. (1993).J. Steroid Biochem. Molec. Biol.,46, 337–347.

    Article  PubMed  CAS  Google Scholar 

  • Stocco, D. M. and Kilgore, M. W. (1988).Biochem. J.,249, 95–103.

    PubMed  CAS  Google Scholar 

  • Stocco, D. M. and Sodeman, T. C. (1991).J. Biol. Chem. 266, 19,731–19,738.

    CAS  Google Scholar 

  • Zar, J. H. (1974). In:Biostatistical Analysis, Prentice-Hall, Englewood Cliffs, NJ, pp. 101–127.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, University of South Florida College of Medicine, 4 Columbia Drive, Rm 529, 33606, Tampa, FL

    Todd W. Sandhoff & Mark P. McLean

  2. Biochemistry and Molecular Biology, University of South Florida College of Medicine, 4 Columbia, Drive, Rm 529, 33606, Tampa, FL

    Todd W. Sandhoff & Mark P. McLean

Authors
  1. Todd W. Sandhoff
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mark P. McLean
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sandhoff, T.W., McLean, M.P. Hormonal regulation of steroidogenic acute regulatory (StAR) protein messenger ribonucleic acid expression in the rat ovary. Endocr 4, 259–267 (1996). https://doi.org/10.1007/BF02738692

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02738692

Key Words

Search

Navigation