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The influence of nitrate, water potential and oxygen tension on nitrogen fixation in detached pea roots

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Abstract

Nodulated pea roots were detached from the plant shoots and exposed to different nitrate concentrations, water potentials, and oxygen tensions. The rates of nitrogen fixation in the detached pea roots following these treatments were determined by the acetylene reduction assay. It was found that increasing the nitrate concentration up to 5 mM brought about reduced nitrogen fixing activity, and this occurred independently of the depressing effects of low water potential and low oxygen tension.

Auszug

Mit Knöllchenbakterien behaftete Erbsenwurzeln wurden von den Pflanzen abgetrennt und verschiedenen Nitratkonzentrationen, Wasserpotentialen und Sauerstoff-Tensionen ausgesetzt. Die Mengen an fixiertem N wurden nach den verschiedenen Behandlungen in den abgetrennten Erbsenwurzeln durch den Azetylen-Reduktionstest bestimmt.

Vermehrte Nitrat-Konzentration bis zu 5 mM brachte eine reduzierte Aktivität in der N-Fixierung hervor. Dies war unabhängig vom depressiven Effekt des niedrigen Wasserpotentials und der niedrigen Sauerstoff-Tension.

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Authors and Affiliations

  1. J.A. Pye Research Centre Haughley, Stowmarket, Suffolk, England

    M. Cowan

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  1. M. Cowan
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Cowan, M. The influence of nitrate, water potential and oxygen tension on nitrogen fixation in detached pea roots. Plant Food Hum Nutr 28, 65–69 (1978). https://doi.org/10.1007/BF01093001

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  • DOI: https://doi.org/10.1007/BF01093001

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