[go: up one dir, main page]
More Web Proxy on the site http://driver.im/ Skip to main content
Springer Nature Link
Log in

Ultraviolet light and ozone stimulate accumulation of salicylic acid, pathogenesis-related proteins and virus resistance in tobacco

  • Published:
Planta Aims and scope Submit manuscript

Abstract

In tobacco (Nicotiana tabacum L. cv. Xanthinc), salicylic acid (SA) levels increase in leaves inoculated by necrotizing pathogens and in healthy leaves located above the inoculated site. Systemic SA increase may trigger disease resistance and synthesis of pathogenesis-related proteins (PR proteins). Here we report that ultraviolet (UV)-C light or ozone induced biochemical responses similar to those induced by necrotizing pathogens. Exposure of leaves to UV-C light or ozone resulted in a transient ninefold increase in SA compared to controls. In addition, in UV-light-irradiated plants, SA increased nearly fourfold to 0.77 μg·g−1 fresh weight in leaves that were shielded from UV light. Increased SA levels were accompanied by accumulation of an SA conjugate and by an increase in the activity of benzoic acid 2-hydroxylase which catalyzes SA biosynthesis. In irradiated and in unirradiated leaves of plants treated with UV light, as well as in plants fumigated with ozone, PR proteins 1a and 1b accumulated. This was paralleled by the appearance of induced resistance to a subsequent challenge with tobacco mosaic virus. The results suggest that UV light, ozone fumigation and tobacco mosaic virus can activate a common signal-transduction pathway that leads to SA and PR-protein accumulation and increased disease resistance.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
£29.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (United Kingdom)

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

PR protein:

pathogenesis-related protein

SA:

salicylic acid

TMV:

tobacco mosaic virus

UV:

ultraviolet

References

  • Apostol, I., Heinstein, P.F., Low, P.S. (1989) Rapid stimulation of an oxidative burst during elicitation of cultured plant cells. Plant Physiol. 90, 109–116

    Google Scholar 

  • Bors, W., Heller, W., Michel, C., Saran, M. (1989) Flavonoids as antioxidants. Methods Enzymol. 186, 343–355

    Google Scholar 

  • Brederode, F.T., Linthorst, H.J.M., Bol, J.F. (1991) Differential induction of acquired resistance and PR gene expression by virus infection, ethephon treatment, UV light and wounding. Plant Molec. Biol. 17, 1117–1125

    Google Scholar 

  • Chappell, J., Hahlbrock, K. (1984) Transcription of plant defense genes in response to UV light or fungal elicitor. Nature 311, 76–78

    Google Scholar 

  • Chester, K.S. (1933) The problem of acquired physiological immunity in plants. Quart. Rev. Biol. 8, 275–324

    Google Scholar 

  • Dixon, R.A., Lamb, C.J. (1990) Molecular communication in interactions between plants and microbial pathogens. Annu. Rev. Plant Physiol. Plant Mol. Biol. 41, 339–367

    Google Scholar 

  • Enyedi, A.J., Raskin, I. (1993) Induction of UDP-glucose:salicylic acid glucosyltransferase activity in tobacco mosaic virus-inoculated tobacco (Nicotiana tabacum) leaves. Plant Physiol. 101, 1375–1380

    Google Scholar 

  • Enyedi, A.J., Yalpani, N., Silverman, P., Raskin, I. (1992) Localization, conjugation, and function of salicylic acid in tobacco during the hypersensitive reaction to tobacco mosaic virus. Proc. Natl. Acad. Sci. USA 89, 2480–2484

    Google Scholar 

  • Ernst, D., Schraudner, M., Langebartels, C., Sandermann, H. (1992) Ozone-induced changes of mRNA levels of β-1,3-glucanase, chitinase and ‘pathogenesis-related’ protein 1b in tobacco plants. Plant Mol. Biol. 20, 673–682

    Google Scholar 

  • Gilpatrick, J.D., Weintraub, M. (1952) An unusual type of protection with the carnation mosaic virus. Science 115, 701–702

    Google Scholar 

  • Heath, R.L. (1988) Biochemical mechanisms of pollutant stress. In: Assessment of crop loss from air pollutants, pp. 259–286, W.W. Heck, O.C. Taylor, D.T. Tingey, eds., Elsevier, London

    Google Scholar 

  • Imbrie, C.W., Murphy, T.M. (1984) Mechanism of photoinactivation of plant plasma membrane ATPase. Photochem. Photobiol. 40, 243–248

    Google Scholar 

  • Kuhn, D.L., Chappell, J., Boudet, A., Hahlbrock, K. (1984) Induction of phenylalanine ammonia-lyase and 4-coumarate:CoA ligase mRNAs in cultured plant cells by UV light and fungal elicitor. Proc. Natl. Acad. Sci. U.S.A. 81, 1102–1106

    Google Scholar 

  • León, J., Yalpani, N., Raskin, I., Lawton, M. (1993) Induction of benzoic acid 2-hydroxylase in virus-inoculated tobacco. Plant Physiol. 103, 323–328

    Google Scholar 

  • Malamy, J., Carr, J.P., Klessig, D.F., Raskin, I. (1990) Salicylic acid — a likely endogenous signal in the resistance response of tobacco to tobacco mosaic virus infection. Science 250, 1002–1004

    Google Scholar 

  • Métraux, J.P., Signer, H., Ryals, J., Ward, E., Wyss-Benz, M., Gaudin, J., Raschdorf, K., Schmid, E., Blum, W., Inverardi, B. (1990) Increase in salicylic acid at the onset of systemic acquired resistance in cucumber. Science 250, 1004–1006

    Google Scholar 

  • Rasmussen, J.B., Hammerschmidt, R., Zook, M.N. (1991) Systemic induction of salicylic acid accumulation in cucumber after inoculation with Pseudomonas syringae pv. syringae. Plant Physiol. 97, 1342–1347

    Google Scholar 

  • Rosemann, D., Heller, W., Sandermann, H. (1991) Biochemical plant responses to ozone. II. Induction of stilbene biosynthesis in Scots pine (Pinus sylvestris L.) seedlings. Plant Physiol. 97, 1280–1286

    Google Scholar 

  • Ross, A.F. (1961a) Systemic acquired resistance induced by localized virus infections in plants. Virology 13, 340–358

    Google Scholar 

  • Ross, A.F. (1961b) Localized acquired resistance to plant virus infection in hypersensitive hosts. Virology 14, 329–339

    Google Scholar 

  • Schraudner, M., Ernst, D., Langebartels, C., Sandermann, H. (1992) Biochemical plant responses to ozone. III. Activation of the defense-related proteins β-1,3-glucanase and chitinase in tobacco leaves. Plant Physiol. 99, 1321–1328

    Google Scholar 

  • Silverman, P., Nuckles, E., Ye, X.S., Kuc, J., Raskin, I. (1993) Salicylic acid, ethylene, and pathogen resistance in tobacco. MPMI 6, 775–781

    Google Scholar 

  • Ward, E.R., Uknes, S.J., Williams, S.C., Dincher, S.S., Wiederhold, D.L., Alexander, D.C., Ahl-Goy, P., Métraux, J.P., Ryals, J.A. (1991) Coordinate gene activity in response to agents that induce systemic acquired resistance. Plant Cell 3, 1085–1094

    Google Scholar 

  • Yalpani, N., Raskin, I. (1993) Salicylic acid: a systemic signal in plant disease resistance. Trends Microbiol. 1, 88–92

    Google Scholar 

  • Yalpani, N., Silverman, P., Wilson, T.M.A., Kleier, S.A., Raskin, I. (1991) Salicylic acid is a systemic signal and an inducer of pathogenesis-related proteins in virus-infected tobacco. Plant Cell 3, 809–818

    Google Scholar 

  • Yalpani, N., Leon, J., Lawton, M.A., Raskin, I. (1993) Pathway of salicylic acid biosynthesis in healthy and virus-inoculated tobacco. Plant Physiol. 103, 315–321

    Google Scholar 

Download references

Author information

Author notes

  1. Nasser Yalpani

    Present address: Pioneer Hi-Bred International, Inc., Plant Breeding Division, 7300 N.W. 62nd Ave., P.O. Box 1004, 50131, Johnston, IA, USA

  2. Alexander J. Enyedi

    Present address: Department of Biological Sciences, Western Michigan University, 49008-3899, Kalamazoo, MI, USA

Authors and Affiliations

  1. AgBiotech Center, Cook College, Rutgers University, 08903-0231, New Brunswick, NJ, USA

    Nasser Yalpani, Alexander J. Enyedi, Jose León & Ilya Raskin

Authors
  1. Nasser Yalpani
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alexander J. Enyedi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jose León
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ilya Raskin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ilya Raskin.

Additional information

This work was financed by grants from the U.S. Department of Agriculture (Competitive Research Grants Office), Division of Energy Biosciences of U.S. Department of Energy, the Rockefeller Foundation, the New Jersey Commission for Science and Technology, and the New Jersey Agricultural Experiment Station.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yalpani, N., Enyedi, A.J., León, J. et al. Ultraviolet light and ozone stimulate accumulation of salicylic acid, pathogenesis-related proteins and virus resistance in tobacco. Planta 193, 372–376 (1994). https://doi.org/10.1007/BF00201815

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00201815

Key words

Search

Navigation