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Physical mapping of Staphylococcus aureus penicillinase plasmid pI524: Characterization of an invertible region

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Summary

The staphylococcal penicillinase plasmid pI524 and a series of derivatives have been extensively mapped by restriction endonuclease digestion and by heteroduplex analysis. We report here the identification of a 2.2 kb region that undergoes a reversible, rec-independent inversion. This sequence is bounded by a pair of inverted repeats 650 base pairs in length, and has asymmetrically located recognition sites for at least three restriction endonucleases. A series of deleted derivatives and one naturally occurring, closely related plasmid, were studied. Two of these retain the inversion; the remainder are incapable of inverting and were all found to be locked in the same orientation of the inversion. The invertible sequence is adjacent to the region of the plasmid encoding β-lactamase (bla); this entire region appears to be transposable and the inversion may be involved in the regulation of β-lactamase expression or in translocation.

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References

  • Allet, B., Bukhari, A.I.: Analysis of bacteriophage Mu- and Lambda-hybrid DNAs by specific endoncleases. J. Mol. Biol. 92, 529–540 (1975)

    Google Scholar 

  • Asheshov, E.H.: The genetics of penicillinase production in Staphylococcus aureus strain PS80. J. Gen. Microbiol. 59, 289–301 (1969)

    Google Scholar 

  • Blin, N., Gabain, A.V., Bujard, H.: Isolation of large molecular weight DNA from agarose gels for further digestion by restriction enzymes. FEBS Letters 53, 84–86 (1975)

    Google Scholar 

  • Broker, T.R., Soll, L., Chow, L.T.: Underwound loops in self-renatured DNA can be diagnostic of inverted duplications and translocated sequences. J. Mol. Biol. 113, 579–589 (1977)

    Google Scholar 

  • Bukhari, A.I., Ambrosio, L.: The invertible segment of bacteriophage Mu DNA determines the adsorption properties of Mu particles. Nature 271, 575–577 (1978)

    Google Scholar 

  • Chopra, I., Bennett, P.M., Lacey, R.W.: A variety of staphylococcal plasmids present as multiple copies. J. Gen. Microbiol. 79, 343–345 (1973)

    Google Scholar 

  • Chow, L.T., Kahmann, R., Kamp, D.: Electron microscopic characterization of DNAs of non-defective deletion mutants of bacteriophage Mu. J. Mol. Biol. 113, 591–609 (1977)

    Google Scholar 

  • Daniell, E., Boram, W., Abelson, J.: Genetic mapping of the inversion loop in bacteriophage Mu DNA. Proc. Natl. Acad. Sci. U.S.A. 70, 2153–2156 (1973)

    Google Scholar 

  • Davis, R.W., Simon, M.N., Davidson, N.: Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. In Methods in enzymology (Grossman, L., and Moldave, K., eds.), Vol. 21D, pp. 413–428. New York: Academic Press 1971

    Google Scholar 

  • Dyke, K.G.H., Richmond, M.H.: Occurrence of various types of penicillinase plasmids among “hospital” staphylococci. J. Clin. Pathol. 20, 75–79 (1967)

    Google Scholar 

  • Greene, P.J., Betlach, M.C., Goodman, H.M., Boyer, H.W.: The EcoRI restriction enonuclease. In Methods in molecular biology-DNA replication and biosynthesis (R. B. Wickner, ed.), Vol. 7, pp. 87–111. New York: Marcel Dekker, Inc. 1974

    Google Scholar 

  • Hsu, M.T., Davidson, N.: Electron microscope heteroduplex study of the heterogeneity of Mu phage and prophage DNA. Virology 58, 229–239 (1974)

    Google Scholar 

  • Kamp, D., Kahmann, R., Zipser, D., Broker, T.R., Chow, L.T.: Inversion of the G DNA segment of phage Mu controls phage infectivity. Nature 271, 577–580 (1978)

    Google Scholar 

  • Kleckner, N.: Translocatable elements in prokaryotes. Cell 11, 11–23 (1977)

    Google Scholar 

  • Morse, L.S., Buchman, T.G., Roizman, B., Schafer, P.: Anatomy of herpes simplex virus DNA. IX. Apparent exclusion of some parental DNA arrangements in the generation of intertypic (HSV-1xHSV-2) recombinants. J. Virol. 24, 231–248 (1977)

    Google Scholar 

  • Murray, K., Murray, N.E.: Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus, influenzae and restriction endonuclease I of Escherichia coli. J. Mol. Biol. 98, 551–564 (1975)

    Google Scholar 

  • Novick, R.P.: Analysis by transduction of mutations affecting penicillinase formation in Staphylococcus aureus. J. Gen. Microbiol. 33, 121–136 (1963)

    Google Scholar 

  • Novick, R.P.: Penicillinase plasmids of Staphylococcus aureus. Fed. Proc. 26, 29–38 (1967a)

    Google Scholar 

  • Novick, R.P.: Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus. Virology 33, 155–166 (1967b)

    Google Scholar 

  • Novick, R.P.: Studies on plasmid replication. III. Isolation and characterization of replication-defective mutants. Mol. Gen. Genet. 135, 131–147 (1974)

    Google Scholar 

  • Novick, R.: Plasmid-protein relaxation complexes in Staphylococcus aureus. J. Bacteriol. 127, 1177–1187 (1976)

    Google Scholar 

  • Novick, R., Bouanchaud, D.: Extrachromosomal nature of drug resistance in Staphylococcus aureus. Ann. N.Y. Acad. Sci. 182, 279–294 (1971)

    Google Scholar 

  • Novick, R.P., Brodsky, R.: Studies on plasmid replication. I. Replication of unestablished plasmis in S. aureus. J. Mol. Biol. 68, 285–302 (1972)

    Google Scholar 

  • Novick, R.P., Clowes, R.C., Cohen, S.N., Curtiss, R., Datta, N., Falkow, S.: Uniform nomenclature for bacterial plasmids: A proposal. Bacteriol. Rev. 40, 168–189 (1976)

    Google Scholar 

  • Novick, R.P., Edelman, I., Schwesinger, M., Gruss, A., Swanson, E., Pattee, P.A.: Genetic translocation in Staphylococcus aureus. Proc. Natl. Acad. Sci. U.S.A. 76, 400–404 (1979a)

    Google Scholar 

  • Novick, R.P., Murphy, E., Gryczan, T.J., Baron, E., Edelman, I.: Penicillinase plasmids of Staphylococcus aureus: Restriction-deletion maps. Plasmid 2, 109–129 (1979b)

    Google Scholar 

  • Ohtsubo, H., Ohtsubo, E.: Repeated DNA sequences in plasmids, phages and bacterial chromosomes. In: DNA insertion clements, plasmids, and episomes (Bukhari, A.I., J.A. Shapiro, S.L. Adhya, eds.), pp. 49–63. Cold Spring Harbor New York: Cold Spring Harbor Press, 1977

    Google Scholar 

  • Peyru, G., Wexler, L.F., Novick, R.P.: Naturally-occurring penicillinase plasmids in Staphylococcus aureus. J. Bacteriol. 98, 215–221 (1969)

    Google Scholar 

  • Richmond, M.: Dominance of the inducible state in strains of Staphylococcus aureus containing two distinct penicillinase plasmids. J. Bacteriol. 90, 370–374 (1965)

    Google Scholar 

  • Richmond, M.: The genetic constitution of certain penicillinase micromutants in Staphylococcus aureus. J. Gen. Microbiol. 45, 51–60 (1966)

    Google Scholar 

  • Rush, M.G., Gordon, C.N., Novick, R.P., Warner, R.C.: Penicillinase plasmid DNA from Staphylococcus aureus. Proc. Natl. Acad. Sci. U.S.A. 63, 1304–1309 (1969)

    Google Scholar 

  • Smith, K., Novick, R.P.: Genetic studies on plasmid linked cadmium resistance in S. aureus. J. Bacteriol. 112, 761–772 (1972)

    Google Scholar 

  • Wyman, L., Goering, R.V., Novick, R.P.: Genetic control of chromosomal and plasmid recombination in Staphylococcus aureus. Genetics 76, 681–702 (1974)

    Google Scholar 

  • Wyman, L., Novick, R.P.: Studies on plasmid replication. IV. Complementation of replication-defective mutants by an incompatibility-deficient plasmid. Mol. Gen. Genet. 135, 149–161 (1974)

    Google Scholar 

  • Zieg, J., Hilman, M., Simon, M.: Regulation of gene expression by site-specific inversion. Cell 15, 237–244 (1978)

    Google Scholar 

  • Zieg, J., Silverman, M., Hilman, M., Simon, M.: Recombinational switch for gene expression. Science 196, 170–172 (1977)

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Plasmid Biology, The Public Health Research Institute of the City of New York, Inc., 455 First Avenue, 10016, New York, New York, USA

    Ellen Murphy & Richard P. Novick

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  1. Ellen Murphy
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  2. Richard P. Novick
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Communicated by W. Arber

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Murphy, E., Novick, R.P. Physical mapping of Staphylococcus aureus penicillinase plasmid pI524: Characterization of an invertible region. Molec. Gen. Genet. 175, 19–30 (1979). https://doi.org/10.1007/BF00267851

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  • DOI: https://doi.org/10.1007/BF00267851

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