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Rapid estimation of potato tuber total protein content with coomassie brilliant blue G-250

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Summary

A new method for measuring protein with Coo-massie Brilliant Blue G-250 has been adapted for use as a screening method in a potato tuber protein improvement breeding program. The method is simple, fast and inexpensive, and has successfully estimated the total protein content of a broad range of tuber genotypes having dissimilar amino acid profiles and tuber maturities. Correlation between the Coomassie method and a modified micro-Kjeldahl method, the standard method used in the potato breeding program, was 0.93. Free amino acids and other compounds which interfere with other methods for measuring protein do not interfere with the Coomassie Brilliant Blue procedure.

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Literature

  • Altschul, A.M.; Yatsu, L.V.; Ory, R.L.; Engleman, E.M.: Seed proteins. Ann. Rev. Plant Physiol. 17, 113–137 (1966)

    Google Scholar 

  • Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Anal. Biochem. 72, 248–254 (1976)

    Google Scholar 

  • Campang, G.C.; Cruz, L.J.; Juliano, B.O.: Free amino acids in the bleeding sap and developing grain of the rice plant. Cereal Chem. 48, 533–539 (1971)

    Google Scholar 

  • Coutrez-Geerinck, D.: The effect of mineral nutrition on the distribution of free amino acids in tubers of cv. Gari. Potato Res. 18, 16–27 (1975)

    Google Scholar 

  • Desborough, S.L.: Potato protein determined by the bromophenol blue dyebinding method and other methods. Potato Res. 18, 273–281 (1975)

    Google Scholar 

  • Desborough, S.; Lauer, F.I.: Improvement of potato protein. II. Selection for protein and yield. Am. Potato J. 54, 371–376 (1977)

    Google Scholar 

  • Desborough, S.; Weiser, C.J.: Protein comparisons in selected Phureja-Haploid tuberosum families. Am. Potato J. 49, 227–233 (1972)

    Google Scholar 

  • Desborough, S.L.; Weiser, C.J.: Improving potato protein. I. Evaluation of selection techniques. Am. Potato J. 51, 185–196 (1974)

    Google Scholar 

  • Hoff, J.E.: A simple method for the approximate determination of soluble protein in potato tubers. Potato Res. 18, 428–432 (1975)

    Google Scholar 

  • Hoff, J.E.; Jones, C.M.; Wilcox, G.E.; Castro, M.D.: The effect of nitrogen fertilization on the composition of the free amino acid pool of potato tubers. Am. Potato J. 48, 390–394 (1971)

    Google Scholar 

  • Johnson, V.A.; Lay, C.L.: Genetic improvement of plant protein. Agric., Food Chem. 22, 558–566 (1974)

    Google Scholar 

  • Kirchenbaum, D.M.: In: Handbook of Biochemistry, Selected Data for Molecular Biology, 2nd ed., (Sober, H., ed.), C-71–C-90. Cleveland: Chemical Rubber Co. 1970

    Google Scholar 

  • Li, P.H.; Sayre, K.D.: The protein, non-protein and total nitrogen in Solanum tuberosum ssp. Andigena potatoes. Am. Potato J. 52, 341–350 (1975)

    Google Scholar 

  • Mica, P.S.; Mertz, E.T.; Glover, D.V.: Studies on corn protein. VIII. Free amino acid content of opaque-2 double mutant. Cereal Chem. 52, 844–848 (1975)

    Google Scholar 

  • Nelson, O.E.: Improvement of plant protein quality. In: Improving Plant Protein by Nuclear Techniques, 43–51. Vienna: International Atomic Energy Agency 1970

    Google Scholar 

  • Perez, C.M.; Campang, G.C.; Esmana, B.V.; Monserrate, R.U.; Juliano, B.O.: Protein metabolism in leaves and developing grains of rices differing in grain protein content. Plant Physiol. 51, 537–542 (1973)

    Google Scholar 

  • Potty, V.H.: Determination of proteins in the presence of phenols and pectins. Anal. Biochem. 29, 535–539 (1969)

    Google Scholar 

  • Snyder, J.C.; Desborough, S.L.; Holm, D.G.: Accumulation of protein, non-protein nitrogen and starch during tuber growth of three potato cultivars. Am. Potato J. 54, 545–555 (1977)

    Google Scholar 

  • Stegemann, H.: Properties of and physiological changes in storage proteins. In: The Chemistry and Biochemistry of Plant Proteins (J.B. Harborne; van Sumere, C.F. eds.), 71–88. London — New York — San Francisco: Acad. Press 1975

    Google Scholar 

  • Stegemann, H.; Loescheke, V2: Index of European potato varieties. Mitteilungen aus der Biologischen Bundesanstalt für Landund Forstwirtschaft Berlin Heft 168, (1976)

  • Swaminathan, M.S.; Austin, A.; Kaul, A.K.; Naik, M.S.: Genetic and agronomic enrichment of the quantity and quality of proteins in cereals and pulses. In: New Approaches to Breeding for Plant Protein, 71–86. Vienna: Intern. Atomic Energy Agency 1969

    Google Scholar 

  • Swaminathan, K.; Sud, K.C.; Kishone, H.: Rapid photometric method for the determination of ‘true protein’ content of potatoes based on non-selective dye-binding capacity. Ind. J. Exp. Biol. 11, 63–64 (1973)

    Google Scholar 

  • Udy, D.C.: Estimation of protein in wheat and flour by ion binding. Cereal Chem. 33, 190–197 (1956)

    Google Scholar 

  • van Gelder, W.M.J.; Krechting, C.F.: A rapid method of estimating the content of coagulable protein in potato tubers. Potato Res. 16, 311–314 (1973)

    Google Scholar 

  • Vigue, J.; Li, P.H.: Correlation between methods to determine the protein content of potato tubers. Hort Science 10, 625–627 (1975)

    Google Scholar 

  • Woodham, A.A.: The effect of nitrogen fertilization on the amino acid composition and nutritive value of cereals. Qual. Plant. 23, 281–291 (1973)

    Google Scholar 

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Communicatest by H.F. Linskens

This research was supported in part by the Rockefeller Foundation. Scientific Journal Series Article # 10,157 of the Minnesota Agricultural Experiment Station

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Snyder, J.C., Desborough, S.L. Rapid estimation of potato tuber total protein content with coomassie brilliant blue G-250. Theoret. Appl. Genetics 52, 135–139 (1978). https://doi.org/10.1007/BF00264747

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  • DOI: https://doi.org/10.1007/BF00264747

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