Abstract
Conductive scaffolds are highly used in tissue engineering for bone defect, nerve regeneration, cardiac tissue constructs and many others. Currently, most methods for monitoring cell activities on scaffolds are destructive and invasive such as histological analysis. The research aimed at sensorizing and characterizing a porous gelatin/chitosan scaffold, hence this “Intelligent Scaffold” can behave as a biosensor for evaluating cell behaviour (cell adhesion, proliferation) along with directing cellular growth. Thus, in this research, three-dimensional (3D) gelatin based scaffold has been transformed into conductive scaffold and both the scaffolds are characterized and compared in terms of their electrical conductivity. Carbon black has been used as a doping material to fabricate a Carbon-Gelatin composite conductive scaffold. The scaffolds are prepared by Freeze drying method and carbon black has been homogeneously embedded throughout the gelatin matrix. The scaffold behaviour was characterized by Bio-impedance Spectroscopy method. The preliminary experimental results showed that the conductivity of carbon-gelatin/chitosan scaffold increases around 10 times as compared to simple gelatin scaffold. Thus, these results elucidated the importance of carbon black clustering for development of a conductive network. This shows that carbon black provides conducting path and hence in future, even a small change of cellular activity can be determined by impedance fluctuation within the scaffold.
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Khan, M.A., Lopomo, N.F., Serpelloni, M., Sardini, E., Sartore, L. (2019). Characterization of Sensorized Porous 3D Gelatin/Chitosan Scaffolds Via Bio-impedance Spectroscopy. In: Andò, B., et al. Sensors. CNS 2018. Lecture Notes in Electrical Engineering, vol 539. Springer, Cham. https://doi.org/10.1007/978-3-030-04324-7_72
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