Abstract
The liver is the systemic hub of lipid metabolism. The excessive accumulation of lipids in hepatocytes, steatosis, is a major clinical concern, whose progressive forms lead to end-stage liver disease. Currently, animal studies are the gold standard in toxicological risk assessment. Fueled by an integration of modern omics technologies, in silico models and in vitro system optimization, a new paradigm in the basis for toxicological risk assessment is emerging away from the use of animals. In recent years, in vitro assays have been developed for the early screening of the steatogenic potential of compounds. The present chapter describes an assay for the intracellular detection of lipids, a high-content screen for the distinction between steatosis and phospholipidosis, a multiparametric high-content screen for steatogenic potential and a liver X receptor reporter cell line.
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Acknowledgements
This work was funded by a European Research Council Starting Grant TMIHCV (project number 242699), a Marie Curie Reintegration Grant microLiverMaturation (project number 248417), the Israel-Japan Ministry of Science (award number 9645), the British Council BIRAX Regenerative Medicine award (number 33BX12HGYN) and HeMiBio, a jointly funded consortium by the European Commission and Cosmetics Europe as part of the SEURAT-1 cluster (project number HEALTH-F5-2010-266777).
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Levy, G., Cohen, M., Nahmias, Y. (2015). In Vitro Cell Culture Models of Hepatic Steatosis. In: Vinken, M., Rogiers, V. (eds) Protocols in In Vitro Hepatocyte Research. Methods in Molecular Biology, vol 1250. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2074-7_29
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DOI: https://doi.org/10.1007/978-1-4939-2074-7_29
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