Abstract
Functional approaches for studying embryonic development have greatly advanced thanks to the CRISPR-Cas9 gene editing technique. Previously practiced in just a few organisms, these knockout techniques are now widely applied. Here we describe simple techniques for applying the CRISPR-Cas9 system to study the development of the nerve cord in the ascidian Phallusia mammillata.
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Acknowledgments
We would like to thank members of the Ascidian BioCell team and Janet Chenevert for helpful comments. We would also like to thank the funding agencies that support our work: the French government funding agency Agence National de la Recherche (ANR “MorCell”: ANR-17-CE 13-0028) and Sorbonne University for supporting the Réseau André Picard. We would also like to thank the European Union’s Horizon 2020 research and innovation programme under grant agreement No 730984, ASSEMBLE Plus project. Finally, we also thank the members of the Service Aquariologie (Laurent Gilletta, Alexandre Jan, and Régis Lasbleiz) for maintaining the animals, and the Institut de la Mer de Villefranche (IMEV) that is supported by EMBRC-France, whose French state funds are managed by the ANR within the Investments of the Future program under reference ANR-10-INBS-02.
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McDougall, A., Hebras, C., Gomes, I., Dumollard, R. (2021). Gene Editing in the Ascidian Phallusia mammillata and Tail Nerve Cord Formation. In: Carroll, D.J., Stricker, S.A. (eds) Developmental Biology of the Sea Urchin and Other Marine Invertebrates. Methods in Molecular Biology, vol 2219. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0974-3_13
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