[go: up one dir, main page]
More Web Proxy on the site http://driver.im/
Skip to main content

Advertisement

Advertisement
Springer Nature Link
Account
Menu
Find a journal Publish with us Track your research
Search
Cart
  1. Home
  2. MRS Advances
  3. Article

Ångström- and Nano-scale Pore-Based Nucleic Acid Sequencing of Current and Emergent Pathogens

  • Open access
  • Published: 01 December 2020
  • Volume 5, pages 2889–2906, (2020)
  • Cite this article
Download PDF

You have full access to this open access article

MRS Advances Aims and scope Submit manuscript
Ångström- and Nano-scale Pore-Based Nucleic Acid Sequencing of Current and Emergent Pathogens
Download PDF
  • Britney A. Shepherd1,
  • Md Rubayat-E Tanjil2,
  • Yunjo Jeong2,
  • Bilgenur Baloğlu3,
  • Jingqiu Liao4 &
  • …
  • Michael Cai Wang1,2 

  • 546 Accesses

  • 2 Altmetric

  • Explore all metrics

An Erratum to this article was published on 29 December 2020

This article has been updated

Abstract

State-of-the-art nanopore sequencing enables rapid and real-time identification of novel pathogens, which has wide application in various research areas and is an emerging diagnostic tool for infectious diseases including COVID-19. Nanopore translocation enables de novo sequencing with long reads (> 10 kb) of novel genomes, which has advantages over existing short-read sequencing technologies. Biological nanopore sequencing has already achieved success as a technology platform but it is sensitive to empirical factors such as pH and temperature. Alternatively, ångström- and nano-scale solid-state nanopores, especially those based on two-dimensional (2D) membranes, are promising next-generation technologies as they can surpass biological nanopores in the variety of membrane materials, ease of defining pore morphology, higher nucleotide detection sensitivity, and facilitation of novel and hybrid sequencing modalities. Since the discovery of graphene, atomically-thin 2D materials have shown immense potential for the fabrication of nanopores with well-defined geometry, rendering them viable candidates for nanopore sequencing membranes. Here, we review recent progress and future development trends of 2D materials and their ångström- and nano-scale pore-based nucleic acid (NA) sequencing including fabrication techniques and current and emerging sequencing modalities. In addition, we discuss the current challenges of translocation-based nanopore sequencing and provide an outlook on promising future research directions.

Article PDF

Download to read the full article text

Similar content being viewed by others

Rapid Detection of Genetic Engineering, Structural Variation, and Antimicrobial Resistance Markers in Bacterial Biothreat Pathogens by Nanopore Sequencing

Article Open access 18 September 2019

The Oxford Nanopore MinION: delivery of nanopore sequencing to the genomics community

Article Open access 25 November 2016

Development of a portable on-site applicable metagenomic data generation workflow for enhanced pathogen and antimicrobial resistance surveillance

Article Open access 11 November 2023
Use our pre-submission checklist

Avoid common mistakes on your manuscript.

Change history

  • 29 December 2020

    An Erratum to this paper has been published: https://doi.org/10.1557/adv.2020.424

References

  1. B. Baloğlu, Z. Chen, V. Elbrecht, T. Braukmann, S. MacDonald, and D. Steinke, “A workflow for accurate metabarcoding using nanopore MinION sequencing 1,” bioRxiv, p. 2020.05.21.108852, May 2020, doi: 10.1101/2020.05.21.108852.

    Google Scholar 

  2. V. L. Dao Thi et al.,, “A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples,” Sci. Transl. Med., vol. 12, no. 556, p. eabc7075, Aug. 2020, doi: 10.1126/scitranslmed.abc7075.

    Google Scholar 

  3. Q. Chen and Z. Liu, “Fabrication and applications of solid-state nanopores,” Sensors (Switzerland), vol. 19, no. 8. MDPI AG, Apr. 02, 2019, doi: 10.3390/s19081886.

    Google Scholar 

  4. U. M. Mirsaidov, D. Wang, W. Timp, and G. Timp, “Molecular diagnostics for personal medicine using a nanopore,” Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology, vol. 2, no. 4. NIH Public Access, pp. 367–381, Jul. 2010, doi: 10.1002/wnan.86.

    CAS  Google Scholar 

  5. G. Guglielmi, “The explosion of new coronavirus tests that could help to end the pandemic,” Nature, vol. 583, no. 7817, pp. 506–509, Jul. 2020, doi: 10.1038/d41586-020-02140-8.

    Article  CAS  Google Scholar 

  6. S. George et al., “DNA Thermo-Protection Facilitates Whole Genome Sequencing of Mycobacteria Direct from Clinical Samples by the Nanopore Platform,” bioRxiv, p. 2020.04.05.026864, Apr. 2020, doi: 10.1101/2020.04.05.026864.

    Google Scholar 

  7. UK Government, “Roll-out of 2 new rapid coronavirus tests ahead of winter - GOV.UK,” 2020. https://www.gov.uk/government/news/roll-out-of-2-new-rapid-coronavirus-tests-ahead-of-winter (accessed Aug. 10, 2020).

  8. “Novel Coronavirus (COVID-19) Overview.” https://nanoporetech.com/covid-19/overview (accessed Sep. 16, 2020).

  9. P. James et al.,, “LamPORE: rapid, accurate and highly scalable molecular screening for SARS-CoV-2 infection, based on nanopore sequencing,” medRxiv, vol. 2020, no. January, p. 2020.08.07.20161737, 2020, doi: 10.1101/2020.08.07.20161737.

  10. M. A. Sutton et al.,, “Radiation Tolerance of Nanopore Sequencing Technology for Life Detection on Mars and Europa,” Sci. Rep., vol. 9, no. 1, pp. 1–10, Dec. 2019, doi: 10.1038/s41598-019-41488-4.

    Article  CAS  Google Scholar 

  11. T. Tucker, M. Marra, and J. M. Friedman, “Massively Parallel Sequencing: The Next Big Thing in Genetic Medicine,” American Journal of Human Genetics, vol. 85, no. 2. Am J Hum Genet, pp. 142–154, Aug. 14, 2009, doi: 10.1016/j.ajhg.2009.06.022.

    Article  CAS  Google Scholar 

  12. J. Yang et al.,, “Photo-induced ultrafast active ion transport through graphene oxide membranes,” 2019. doi: 10.1038/s41467-019-09178-x.

    Book  Google Scholar 

  13. S. S. Johnson, E. Zaikova, D. S. Goerlitz, Y. Bai, and S. W. Tighe, “Real-time DNA sequencing in the antarctic dry valleys using the Oxford nanopore sequencer,” J. Biomol. Tech., vol. 28, no. 1, pp. 2–7, Apr. 2017, doi: 10.7171/jbt.17-2801-009.

    Article  Google Scholar 

  14. R. Meier, W. Wong, A. Srivathsan, and M. Foo, “$1 DNA barcodes for reconstructing complex phenomes and finding rare species in specimen-rich samples,” Cladistics, vol. 32, no. 1, pp. 100–110, Feb. 2016, doi: 10.1111/cla.12115.

    Article  Google Scholar 

  15. A. Viehweger et al.,, “Direct RNA nanopore sequencing of full-length coronavirus genomes provides novel insights into structural variants and enables modification analysis,” bioRxiv, p. 483693, Aug. 2018, doi: 10.1101/483693.

    Google Scholar 

  16. S. Howorka, “Building membrane nanopores,” Nature Nanotechnology, vol. 12, no. 7. Nature Publishing Group, pp. 619–630, Jul. 01, 2017, doi: 10.1038/nnano.2017.99.

    Article  CAS  Google Scholar 

  17. Y. Liu and L. Yobas, “Slowing DNA Translocation in a Nanofluidic Field-Effect Transistor,” ACS Nano, vol. 10, no. 4, pp. 3985–3994, 2016, doi: 10.1021/acsnano.6b00610.

    Article  CAS  Google Scholar 

  18. S. W. Kowalczyk, A. Y. Grosberg, Y. Rabin, and C. Dekker, “Modeling the conductance and DNA blockade of solid-state nanopores,” Nanotechnology, vol. 22, no. 31, 2011, doi: 10.1088/0957-4484/22/31/315101.

    Google Scholar 

  19. L. Liang, J. W. Shen, Z. Zhang, and Q. Wang, “DNA sequencing by two-dimensional materials: As theoretical modeling meets experiments,” Biosensors and Bioelectronics, vol. 89. Elsevier Ltd, pp. 280–292, Mar. 15, 2017, doi: 10.1016/j.bios.2015.12.037.

    Article  CAS  Google Scholar 

  20. S. J. Heerema, G. F. Schneider, M. Rozemuller, L. Vicarelli, H. W. Zandbergen, and C. Dekker, “1/F Noise in Graphene Nanopores,” Nanotechnology, vol. 26, no. 7, p. 074001, Feb. 2015, doi: 10.1088/0957-4484/26/7/074001.

    Article  CAS  Google Scholar 

  21. D. Sarkar, W. Liu, X. Xie, A. C. Anselmo, S. Mitragotri, and K. Banerjee, “MoS2 field-effect transistor for next-generation label-free biosensors,” ACS Nano, vol. 8, no. 4, pp. 3992–4003, 2014, doi: 10.1021/nn5009148.

    Article  CAS  Google Scholar 

  22. M. Mojtabavi, A. VahidMohammadi, W. Liang, M. Beidaghi, and M. Wanunu, “Single-Molecule Sensing Using Nanopores in Two-Dimensional Transition Metal Carbide (MXene) Membranes,” ACS Nano, p. acsnano.8b08017, 2019, doi: 10.1021/acsnano.8b08017.

    Google Scholar 

  23. M. R.-E. Tanjil, Y. Jeong, Z. Yin, W. Panaccione, and M. C. Wang, “Ångstrom-Scale, Atomically Thin 2D Materials for Corrosion Mitigation and Passivation,” Coatings, vol. 9, no. 2, p. 133, Feb. 2019, doi: 10.3390/coatings9020133.

    Article  CAS  Google Scholar 

  24. K. Liu, J. Feng, A. Kis, and A. Radenovic, “Atomically Thin Molybdenum Disulfide Nanopores with High Sensitivity for DNA Translocation,” 2014, doi: 10.1021/nn406102h.

    Book  Google Scholar 

  25. S. Garaj, W. Hubbard, A. Reina, J. Kong, D. Branton, and J. A. Golovchenko, “Graphene as a subnanometre trans-electrode membrane,” Nature, vol. 467, no. 7312, pp. 190–193, Sep. 2010, doi: 10.1038/nature09379.

    Article  CAS  Google Scholar 

  26. S. Thomas, A. C. Rajan, M. R. Rezapour, and K. S. Kim, “In search of a two-dimensional material for DNA sequencing,” J. Phys. Chem. C, vol. 118, no. 20, pp. 10855–10858, 2014, doi: 10.1021/jp501711d.

    Article  CAS  Google Scholar 

  27. S. Garaj, W. Hubbard, A. Reina, J. Kong, D. Branton, and J. A. Golovchenko, “Graphene as a subnanometre trans-electrode membrane,” Nature, vol. 467, no. 7312, pp. 190–193, Sep. 2010, doi: 10.1038/nature09379.

    Article  CAS  Google Scholar 

  28. S. Su, X. Guo, Y. Fu, Y. Xie, X. Wang, and J. Xue, “Origin of nonequilibrium 1/: F noise in solid-state nanopores,” Nanoscale, vol. 12, no. 16, pp. 8975–8981, Apr. 2020, doi: 10.1039/c9nr09829a.

    Article  CAS  Google Scholar 

  29. A. W. Robertson et al.,, “Spatial control of defect creation in graphene at the nanoscale,” Nat. Commun., vol. 3, no. 1, p. 1144, Jan. 2012, doi: 10.1038/ncomms2141.

    Article  CAS  Google Scholar 

  30. C. M. Rochman, “The complex mixture, fate and toxicity of chemicals associated with plastic debris in the marine environment,” in Marine Anthropogenic Litter, Cham: Springer International Publishing, 2015, pp. 117–140.

    Chapter  Google Scholar 

  31. C. J. Russo and J. A. Golovchenko, “Atom-by-atom nucleation and growth of graphene nanopores.,” Proc. Natl. Acad. Sci. U.S.A., vol. 109, no. 16, pp. 5953-7, Apr. 2012, doi: 10.1073/pnas.1119827109.

    Article  CAS  Google Scholar 

  32. G. Danda et al.,, “Monolayer WS2 Nanopores for DNA Translocation with Light-Adjustable Sizes,” ACS Nano, vol. 11, no. 2, pp. 1937–1945, Feb. 2017, doi: 10.1021/acsnano.6b08028.

    Article  CAS  Google Scholar 

  33. J. Feng et al.,, “Electrochemical reaction in single layer MoS2: Nanopores opened atom by atom,” Nano Lett., vol. 15, no. 5, pp. 3431–3438, 2015, doi: 10.1021/acs.nanolett.5b00768.

    Article  CAS  Google Scholar 

  34. A. T. Kuan, B. Lu, P. Xie, T. Szalay, and J. A. Golovchenko, “Electrical pulse fabrication of graphene nanopores in electrolyte solution,” Appl. Phys. Lett., vol. 106, no. 20, May 2015, doi: 10.1063/1.4921620.

    Google Scholar 

  35. M. Jain, H. E. Olsen, B. Paten, and M. Akeson, “The Oxford Nanopore MinION: delivery of nanopore sequencing to the genomics community,” Genome Biol., vol. 17, no. 1, pp. 1–11, Dec. 2016, doi: 10.1186/s13059-016-1103-0.

    Article  CAS  Google Scholar 

  36. C. Wloka, N. L. Mutter, M. Soskine, and G. Maglia, “Alpha-Helical Fragaceatoxin C Nanopore Engineered for Double-Stranded and Single-Stranded Nucleic Acid Analysis,” Angew. Chemie - Int. Ed., vol. 55, no. 40, pp. 12494–12498, Sep. 2016, doi: 10.1002/anie.201606742.

    Article  CAS  Google Scholar 

  37. J. Larkin, R. Henley, D. C. Bell, T. Cohen-Karni, J. K. Rosenstein, and M. Wanunu, “Slow DNA transport through nanopores in hafnium oxide membranes,” ACS Nano, vol. 7, no. 11, pp. 10121–10128, Nov. 2013, doi: 10.1021/nn404326f.

    Article  CAS  Google Scholar 

  38. H. Qiu, A. Sarathy, K. Schulten, and J. P. Leburton, “Detection and mapping of DNA methylation with 2D material nanopores,” npj 2D Mater. Appl., vol. 1, no. 1, p. 3, Dec. 2017, doi: 10.1038/s41699-017-0005-7.

    Article  Google Scholar 

  39. M. Belkin, S. H. Chao, M. P. Jonsson, C. Dekker, and A. Aksimentiev, “Plasmonic Nanopores for Trapping, Controlling Displacement, and Sequencing of DNA,” ACS Nano, vol. 9, no. 11, pp. 10598–10611, Nov. 2015, doi: 10.1021/acsnano.5b04173.

    Article  CAS  Google Scholar 

  40. J. M. Yang et al.,, “Surface-Enhanced Raman Scattering Probing the Translocation of DNA and Amino Acid through Plasmonic Nanopores,” Anal. Chem., vol. 91, no. 9, pp. 6275–6280, May 2019, doi: 10.1021/acs.analchem.9b01045.

    Article  CAS  Google Scholar 

  41. M. Wanunu, W. Morrison, Y. Rabin, A. Y. Grosberg, and A. Meller, “Electrostatic focusing of unlabelled DNA into nanoscale pores using a salt gradient,” Nat. Nanotechnol., vol. 5, no. 2, pp. 160–165, Dec. 2010, doi: 10.1038/nnano.2009.379.

    Article  CAS  Google Scholar 

  42. S. W. Kowalczyk, D. B. Wells, A. Aksimentiev, and C. Dekker, “Slowing down DNA translocation through a nanopore in lithium chloride,” Nano Lett., vol. 12, no. 2, pp. 1038–1044, 2012, doi: 10.1021/nl204273h.

    Article  CAS  Google Scholar 

  43. M. Wanunu, J. Sutin, B. McNally, A. Chow, and A. Meller, “DNA translocation governed by interactions with solid-state nanopores,” Biophys. J., vol. 95, no. 10, pp. 4716–4725, Nov. 2008, doi: 10.1529/biophysj.108.140475.

    Article  CAS  Google Scholar 

  44. B. Luan, G. Stolovitzky, and G. Martyna, “Slowing and controlling the translocation of DNA in a solid-state nanopore,” Nanoscale, vol. 4, no. 4, pp. 1068–1077, Feb. 2012, doi: 10.1039/c1nr11201e.

    Article  CAS  Google Scholar 

  45. H. Peng and X. S. Ling, “Reverse DNA translocation through a solid-state nanopore by magnetic tweezers,” Nanotechnology, vol. 20, no. 18, p. 185101, Apr. 2009, doi: 10.1088/0957-4484/20/18/185101.

    Article  CAS  Google Scholar 

  46. S. Van Dorp, U. F. Keyser, N. H. Dekker, C. Dekker, and S. G. Lemay, “Origin of the electrophoretic force on DNA in solid-state nanopores,” Nat. Phys., vol. 5, no. 5, pp. 347–351, Mar. 2009, doi: 10.1038/nphys1230.

    Article  CAS  Google Scholar 

  47. R. Balasubramanian et al.,, “DNA Translocation through Hybrid Bilayer Nanopores,” J. Phys. Chem. C, vol. 123, no. 18, pp. 11908–11916, May 2019, doi: 10.1021/acs.jpcc.9b00399.

    Article  CAS  Google Scholar 

  48. M. H. Lee et al.,, “A low-noise solid-state nanopore platform based on a highly insulating substrate,” Sci. Rep., vol. 4, Dec. 2014, doi: 10.1038/srep07448.

  49. G. F. Schneider et al.,, “DNA translocation through graphene nanopores,” Nano Lett., vol. 10, no. 8, pp. 3163–3167, 2010, doi: 10.1021/nl102069z.

    Article  CAS  Google Scholar 

  50. S. J. Heerema, L. Vicarelli, S. Pud, R. N. Schouten, H. W. Zandbergen, and C. Dekker, “Probing DNA Translocations with Inplane Current Signals in a Graphene Nanoribbon with a Nanopore,” ACS Nano, vol. 12, no. 3, pp. 2623–2633, Mar. 2018, doi: 10.1021/acsnano.7b08635.

    Article  CAS  Google Scholar 

  51. T. Haynes, I. P. S. Smith, E. J. Wallace, J. L. Trick, M. S. P. Sansom, and S. Khalid, “Electric-field-driven translocation of ssDNA through hydrophobic nanopores,” ACS Nano, vol. 12, no. 8, pp. 8208–8213, 2018, doi: 10.1021/acsnano.8b03365.

    Article  CAS  Google Scholar 

  52. M. Graf, M. Lihter, D. Altus, S. Marion, and A. Radenovic, “Transverse Detection of DNA Using a MoS2 Nanopore,” Nano Lett., vol. 19, no. 12, pp. 9075–9083, 2019, doi: 10.1021/acs.nanolett.9b04180.

    Article  CAS  Google Scholar 

  53. “SERS/TERS.” https://www.renishaw.com/en/sers-ters—25811 (accessed Sep. 18, 2020).

  54. J. Cao et al.,, “SERS Detection of Nucleobases in Single Silver Plasmonic Nanopores,” ACS sensors, vol. 5, no. 7, pp. 2198–2204, Jul. 2020, doi: 10.1021/acssensors.0c00844.

    Article  CAS  Google Scholar 

  55. J. A. Huang et al.,, “SERS discrimination of single DNA bases in single oligonucleotides by electro-plasmonic trapping,” Nat. Commun., vol. 10, no. 1, pp. 1–10, Dec. 2019, doi: 10.1038/s41467-019-13242-x.

    Article  CAS  Google Scholar 

  56. C. Chen et al.,, “High spatial resolution nanoslit SERS for single-molecule nucleobase sensing,” Nat. Commun., vol. 9, no. 1, pp. 1–9, Dec. 2018, doi: 10.1038/s41467-018-04118-7.

    Article  CAS  Google Scholar 

  57. Z. He et al.,, “Tip-Enhanced Raman Imaging of Single-Stranded DNA with Single Base Resolution,” J. Am. Chem. Soc., vol. 141, no. 2, pp. 753–757, 2019, doi: 10.1021/jacs.8b11506.

    Article  CAS  Google Scholar 

  58. J. D. Spitzberg, A. Zrehen, X. F. van Kooten, and A. Meller, “Plasmonic-Nanopore Biosensors for Superior Single-Molecule Detection,” Adv. Mater., vol. 31, no. 23, p. 1900422, Jun. 2019, doi: 10.1002/adma.201900422.

    Article  CAS  Google Scholar 

  59. D. Garoli et al.,, “Hybrid plasmonic nanostructures based on controlled integration of MoS2 flakes on metallic nanoholes,” Nanoscale, vol. 10, no. 36, pp. 17105–17111, Sep. 2018, doi: 10.1039/c8nr05026k.

    Article  CAS  Google Scholar 

  60. D. Garoli, H. Yamazaki, N. MacCaferri, and M. Wanunu, “Plasmonic Nanopores for Single-Molecule Detection and Manipulation: Toward Sequencing Applications,” Nano Lett., vol. 19, no. 11, pp. 7553–7562, 2019, doi: 10.1021/acs.nanolett.9b02759.

    Article  CAS  Google Scholar 

  61. L. Restrepo-Pérez, C. Joo, and C. Dekker, “Paving the way to single-molecule protein sequencing,” Nature Nanotechnology, vol. 13, no. 9. Nature Publishing Group, pp. 786–796, Sep. 01, 2018, doi: 10.1038/s41565-018-0236-6.

    Article  CAS  Google Scholar 

  62. A. Srivathsan et al.,, “A MinIONTM-based pipeline for fast and cost-effective DNA barcoding,” Mol. Ecol. Resour., vol. 18, no. 5, pp. 1035–1049, Sep. 2018, doi: 10.1111/1755-0998.12890.

    Article  CAS  Google Scholar 

  63. D. P. Hoogerheide, S. Garaj, and J. A. Golovchenko, “Probing surface charge fluctuations with solid-state nanopores,” Phys. Rev. Lett., vol. 102, no. 25, 2009, doi: 10.1103/PhysRevLett.102.256804.

    Google Scholar 

  64. M. R. Powell et al.,, “Noise properties of rectifying nanopores,” J. Phys. Chem. C, vol. 115, no. 17, pp. 8775–8783, May 2011, doi: 10.1021/jp2016038.

    Article  CAS  Google Scholar 

  65. Z. Y. Zhang, Y. S. Deng, H. B. Tian, H. Yan, H. L. Cui, and D. Q. Wang, “Noise analysis of monolayer graphene nanopores,” Int. J. Mol. Sci., vol. 19, no. 9, p. 2639, Sep. 2018, doi: 10.3390/ijms19092639.

    Article  CAS  Google Scholar 

  66. A. Fragasso, S. Schmid, and C. Dekker, “Comparing Current Noise in Biological and Solid-State Nanopores,” ACS Nano, vol. 14, no. 2, pp. 1338–1349, 2020, doi: 10.1021/acsnano.9b09353.

    Article  CAS  Google Scholar 

  67. A. R. Hall, A. Scott, D. Rotem, K. K. Mehta, H. Bayley, and C. Dekker, “Hybrid pore formation by directed insertion of α-haemolysin into solid-state nanopores,” Nat. Nanotechnol., vol. 5, no. 12, pp. 874–877, 2010, doi: 10.1038/nnano.2010.237.

    Article  CAS  Google Scholar 

  68. E. M. Nelson, H. Li, and G. Timp, “Direct, concurrent measurements of the forces and currents affecting DNA in a nanopore with comparable topography,” ACS Nano, vol. 8, no. 6, pp. 5484–5493, Jun. 2014, doi: 10.1021/nn405331t.

    Article  CAS  Google Scholar 

  69. U. F. Keyser et al.,, “Direct force measurements on DNA in a solid-state nanopore,” Nat. Phys., vol. 2, no. 7, pp. 473–477, Jul. 2006, doi: 10.1038/nphys344.

    Article  CAS  Google Scholar 

  70. A. B. Farimani, P. Dibaeinia, and N. R. Aluru, “DNA origami-graphene hybrid nanopore for DNA detection,” ACS Appl. Mater. Interfaces, vol. 9, no. 1, pp. 92–100, 2017, doi: 10.1021/acsami.6b11001.

    Article  CAS  Google Scholar 

  71. M. T. Hwang et al.,, “Ultrasensitive detection of nucleic acids using deformed graphene channel field effect biosensors,” Nat. Commun., vol. 11, no. 1, 2020, doi: 10.1038/s41467-020-15330-9.

    Google Scholar 

  72. V. Shukla, N. K. Jena, A. Grigoriev, and R. Ahuja, “Prospects of Graphene-hBN Heterostructure Nanogap for DNA Sequencing,” ACS Appl. Mater. Interfaces, vol. 9, no. 46, pp. 39945–39952, 2017, doi: 10.1021/acsami.7b06827.

    Article  CAS  Google Scholar 

  73. S. K. Min, W. Y. Kim, Y. Cho, and K. S. Kim, “Fast DNA sequencing with a graphene-based nanochannel device,” Nat. Nanotechnol., vol. 6, no. 3, pp. 162–165, 2011, doi: 10.1038/nnano.2010.283.

    Article  CAS  Google Scholar 

  74. B. Luan and R. Zhou, “Single-File Protein Translocations through Graphene-MoS2 Heterostructure Nanopores,” J. Phys. Chem. Lett., vol. 9, no. 12, pp. 3409–3415, Jun. 2018, doi: 10.1021/acs.jpclett.8b01340.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medical Engineering, University of South Florida, 4202 E. Fowler Avenue, Tampa, Florida, 33620, USA

    Britney A. Shepherd & Michael Cai Wang

  2. Department of Mechanical Engineering, University of South Florida, 4202 E. Fowler Avenue, Tampa, Florida, 33620, USA

    Md Rubayat-E Tanjil, Yunjo Jeong & Michael Cai Wang

  3. Centre for Biodiversity Genomics, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G2W1, Canada

    Bilgenur Baloğlu

  4. Department of Systems Biology, Columbia University, 1130 St. Nicholas Avenue, New York, New York, 10032, USA

    Jingqiu Liao

Authors
  1. Britney A. Shepherd
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Md Rubayat-E Tanjil
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yunjo Jeong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bilgenur Baloğlu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jingqiu Liao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Michael Cai Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Michael Cai Wang.

Additional information

*These authors contributed equally to this work.

Rights and permissions

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Shepherd, B.A., Tanjil, M.RE., Jeong, Y. et al. Ångström- and Nano-scale Pore-Based Nucleic Acid Sequencing of Current and Emergent Pathogens. MRS Advances 5, 2889–2906 (2020). https://doi.org/10.1557/adv.2020.402

Download citation

  • Published: 01 December 2020

  • Issue Date: November 2020

  • DOI: https://doi.org/10.1557/adv.2020.402

Share this article

Anyone you share the following link with will be able to read this content:

Sorry, a shareable link is not currently available for this article.

Provided by the Springer Nature SharedIt content-sharing initiative

Use our pre-submission checklist

Avoid common mistakes on your manuscript.

Advertisement

Search

Navigation

  • Find a journal
  • Publish with us
  • Track your research

Discover content

  • Journals A-Z
  • Books A-Z

Publish with us

  • Journal finder
  • Publish your research
  • Open access publishing

Products and services

  • Our products
  • Librarians
  • Societies
  • Partners and advertisers

Our imprints

  • Springer
  • Nature Portfolio
  • BMC
  • Palgrave Macmillan
  • Apress
  • Your US state privacy rights
  • Accessibility statement
  • Terms and conditions
  • Privacy policy
  • Help and support
  • Cancel contracts here

Not affiliated

Springer Nature

© 2025 Springer Nature