Genome-wide analysis of the HSP۹۰ gene family and their roles in soybean growth and development

Heat shock protein of ۹۰ kDa or HSP۹۰ plays an important dynamic role in regulating biotic and abiotic stresses through multiple functional mechanisms. The present study aimed to perform a comprehensive analysis of the HSP۹۰ gene family in soybean. In total, ۲۰ HSP۹۰ genes from soybean were identified and showed unequal distribution on the ۱۳ chromosomes. The evolutionary tree divided these genes into three main groups based on their subcellular localization. In Group I, nearly all of the HSP۹۰ genes are distributed in the nucleus or cytoplasm. In Group II, the HSP۹۰s were mostly classified in the endoplasmic reticulum. HSP۹۰ genes were exclusively found in the mitochondria or chloroplast in Group III. Phylogenetic relationships have shown that genes in similar subgroups have the same exon-intron structure and number of introns. Glyma۱۴g۲۱۹۷۰۰, Glyma۱۷g۲۵۸۷۰۰, and Glyma۰۷G۲۰۷۶۰۰ were identified as hub proteins based on their high degrees of interaction. In addition, Glyma۰۲g۳۰۲۵۰۰, Glyma۰۸g۳۳۲۹۰۰, Glyma۱۴g۲۱۹۷۰۰, Glyma۱۷g۲۵۸۷۰۰, and Glyma۱۸g۰۷۴۱۰۰ genes displayed high expression levels in all of the tissues at different developmental stages. These findings provide a complete overview of the GmHSP۹۰ gene family classification and evolution, which can help to identify the functional properties of the HSP۹۰ genes in soybean growth and development.