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Addgene: The First Twenty Years

By Rachel Leeson

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A cartoon overlayed on several crystal structures showing the parts of the prime editor: the Cas9 nickase domain, the reverse transcriptase domain, and the pegRNA.
Graphic showing different injection routes in mice; see text for details
Map of the world, with all but five countries colored in blue. The countries colored in blue viewed the Addgene blog in 2023.
Alt text: A schematic illustration of the CRISPR/Cas9-based approaches mentioned above. The first illustration (A) shows a DSB made at a transcription start site upstream of an exon. The second illustration (B) shows a DSB made at splice sites. The third illustration (C) shows a DSB made upstream and downstream of an exon to fully remove a genomic fragment. The fourth illustration (D) shows a DSB made within an exon to insert a synthetic polyadenylation signal. 

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