Sweeping away protein aggregation with entropic bristles: intrinsically disordered protein fusions enhance soluble expression (Q34077737)

30 July 2017

title

Sweeping away protein aggregation with entropic bristles: intrinsically disordered protein fusions enhance soluble expression (English)

1 reference

30 July 2017

1 reference

Vladimir N Uversky

1 reference

30 July 2017

Aaron A Santner

1 reference

30 July 2017

Carrie H Croy

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30 July 2017

Farha H Vasanwala

1 reference

30 July 2017

Ya-Yue J Van

1 reference

30 July 2017

A Keith Dunker

1 reference

30 July 2017

language of work or name

English

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publication date

5 September 2012

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30 July 2017

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30 July 2017

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30 July 2017

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30 July 2017

page(s)

7250-7262

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describes a project that uses

30 July 2017

ImageJ

1 reference

11 June 2022

https://www.ebi.ac.uk/europepmc/webservices/rest/PMC4141500/fullTextXML

inferred from PubMed Central ID database lookup

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5 July 2018

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Expression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin-like polypeptide fusion with an oligohistidine fusion

5 July 2018

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5 July 2018

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5 July 2018

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5 July 2018

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5 July 2018

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5 July 2018

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5 July 2018

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5 July 2018

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5 July 2018

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5 July 2018

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5 July 2018

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5 July 2018

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Structural analysis of regulatory protein domains using GST-fusion proteins

5 July 2018

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Escherichia coli maltose-binding protein as a molecular chaperone for recombinant intracellular cytoplasmic single-chain antibodies.

5 July 2018

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5 July 2018

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Intrinsically disordered protein

5 July 2018

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4141500

Sequence complexity of disordered protein

5 July 2018

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Structural proteomics: prospects for high throughput sample preparation

5 July 2018

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5 July 2018

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Structural proteomics of an archaeon

5 July 2018

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Using an AraC-based three-hybrid system to detect biocatalysts in vivo

5 July 2018

1 reference

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Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4141500

The molecular structure of green fluorescent protein

5 July 2018

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Chaperone properties of the bacterial periplasmic substrate-binding proteins

5 July 2018

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5 July 2018

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5 July 2018

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5 July 2018

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A simple method for displaying the hydropathic character of a protein

5 July 2018

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Multiple joined genes prevent product degradation in Escherichia coli

5 July 2018

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Glutathione S-transferases. The first enzymatic step in mercapturic acid formation

5 July 2018

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Glutathione transferases--structure and catalytic activity

5 July 2018

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5 July 2018

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Secretion of heterologous gene products to the culture medium of Escherichia coli

5 July 2018

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5 July 2018

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Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

5 July 2018

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Extracting hydrophobic free energies from experimental data: relationship to protein folding and theoretical models

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4141500

Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin

5 July 2018

1 reference

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Deg phenotype of Escherichia coli lon mutants

5 July 2018

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5 July 2018

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The use of Group 3 LEA proteins as fusion partners in facilitating recombinant expression of recalcitrant proteins in E. coli

28 October 2018

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Close encounters of the third kind: disordered domains and the interactions of proteins

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4141500

Functional dissection of hydrophilins during in vitro freeze protection

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4141500

Some characteristics of protein precipitation by salts

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4141500

A biologically active angiogenesis inhibitor, human serum albumin-TIMP-2 fusion protein, secreted from Saccharomyces cerevisiae.

28 October 2018

1 reference

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Protein solubility: sequence based prediction and experimental verification.

28 October 2018

1 reference

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Structural and functional insights into the regulation of Arabidopsis AGC VIIIa kinases.

28 October 2018

1 reference

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Structural investigation of disordered stress proteins. Comparison of full-length dehydrins with isolated peptides of their conserved segments

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4141500

Two different late embryogenesis abundant proteins from Arabidopsis thaliana contain specific domains that inhibit Escherichia coli growth

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4141500

Escherichia coli fusion carrier proteins act as solubilizing agents for recombinant uncoupling protein 1 through interactions with GroEL.

28 October 2018

1 reference

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Expression, refolding, and characterization of human soluble BAFF synthesized in Escherichia coli

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4141500

Comparative studies on the structure and stability of fluorescent proteins EGFP, zFP506, mRFP1, "dimer2", and DsRed1.

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4141500

Protein aggregation during overexpression limited by peptide extensions with large net negative charge

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4141500

Soluble expression of aggregating proteins by covalent coupling to the ribosome

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4141500

Refolding and purification of unprocessed porcine myostatin expressed in Escherichia coli

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4141500

Effect of protein fusion on the transition temperature of an environmentally responsive elastin-like polypeptide: a role for surface hydrophobicity?

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4141500

Mining the structural genomics pipeline: identification of protein properties that affect high-throughput experimental analysis

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4141500

Production and purification of refolded recombinant human IL-7 from inclusion bodies

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4141500

Enhancement of cold tolerance and inhibition of lipid peroxidation by citrus dehydrin in transgenic tobacco

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4141500

Conformation of a group 2 late embryogenesis abundant protein from soybean. Evidence of poly (L-proline)-type II structure

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4141500

Antitumor activity of interleukin-21 prepared by novel refolding procedure from inclusion bodies expressed in Escherichia coli.

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4141500

Distinct roles of the N-terminal-binding domain and the C-terminal-solubilizing domain of alpha-synuclein, a molecular chaperone

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4141500

Specific aggregation of partially folded polypeptide chains: The molecular basis of inclusion body composition

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4141500

A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm.

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4141500

Arabidopsis Cor15am is a chloroplast stromal protein that has cryoprotective activity and forms oligomers

28 October 2018

1 reference

12 December 2020

Predicting the solubility of recombinant proteins in Escherichia coli

1 reference

12 December 2020

The protein non-folding problem: amino acid determinants of intrinsic order and disorder

1 reference

12 December 2020

Generating fusions to glutathione S-transferase for protein studies

1 reference

12 December 2020

Dehydrins in cold-acclimated apices of birch (Betula pubescens ehrh. ): production, localization and potential role in rescuing enzyme function during dehydration

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12 December 2020

Fusions to maltose-binding protein: control of folding and solubility in protein purification

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12 December 2020

Functional protein domains from the thermally driven motion of polypeptide chains: a proposal

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12 December 2020

Recovery of polypeptides cleaved from purified calmodulin-binding peptide fusion proteins

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12 December 2020

New fusion protein systems designed to give soluble expression in Escherichia coli

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12 December 2020

Predicting Protein Disorder for N-, C-, and Internal Regions

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12 December 2020

Folding of green fluorescent protein and the cycle3 mutant

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12 December 2020

Identifiers

DOI

10.1021/BI300653M

1 reference

30 July 2017

1 reference

30 July 2017

1 reference