Transcriptomics articles from across Nature Portfolio

The Human Cell Atlas is yielding detailed maps of human tissues and systems throughout life, along with methods to handle single-cell data. Four scientists reflect on how the project is transforming our understanding of human biology.

This Review provides a comprehensive and detailed discussion about how to build and use single-cell atlases.

Single-cell multiomics can provide broad insights into gene/protein regulatory networks and cellular diversity. Here, authors develop nanoSPLITS, a nanodroplet splitting approach for global profiling of the transcriptome and proteome from same single cells via RNA sequencing and mass spectrometry-based proteomics.

This analysis of single-cell RNA sequencing data from peripheral blood mononuclear cells for 474 individuals of diverse Asian ancestries in the Asian Immune Diversity Atlas links cell-type-specific splicing variation with autoimmune and inflammatory disease risk.

The Human Cell Atlas is yielding detailed maps of human tissues and systems throughout life, along with methods to handle single-cell data. Four scientists reflect on how the project is transforming our understanding of human biology.

In this Tools of the Trade article, Andrew Russell describes Slide-tags, a method for scalable, spatially resolved single-nucleus sequencing.

In this work, we demonstrate the transcriptional networks that link breast cancer subtypes and their cells of origin, and how transcriptional signatures differ between benign cells and cancer.