Figure 5
From: Goblet Cell Hyperplasia Requires High Bicarbonate Transport To Support Mucin Release
Effect of anion transport inhibitors.
(A,B) Representative short-circuit current recordings and bar graphs reporting the effect of bumetanide and S0859 on CFTR-dependent currents in cells under control conditions (A) or treated with IL-4 for 72 hrs (B). The value of inhibition reported in bar graphs is calculated from the total CFTR current (current activated by CPT-cAMP minus the current remaining after CFTRinh-172). B: bumetanide. B + S: bumetanide + S0859. *p < 0.05; ***p < 0.001 vs. bumetanide alone (n = 8 per condition; BE37 cells). (C,D) Representative short-circuit current recordings and bar graphs reporting the effect of bumetanide and S0859 on UTP-induced currents in cells under control conditions (C) or treated with IL-4 for 72 hrs (D). Activity of TMEM16A/CaCC was quantified as current peak or as AUC. *p < 0.05; **p < 0.01; ***p < 0.001 vs. currents with no inhibitors. #p < 0.05; ##p < 0.01; ###p < 0.001 vs. currents with bumetanide alone (n = 8 per condition).