Extended Data Fig. 8: ILC2/type 2 immune cell enriched human melanoma tumors are associated with increased eosinophil infiltration and gene expression.
From: Blockade of the co-inhibitory molecule PD-1 unleashes ILC2-dependent antitumor immunity in melanoma
a and b, Analysis of the impact of tumor ILC2/type 2 immune cell and eosinophil infiltration on melanoma patient survival using the publicly available TCGA database. a, Dot plot of the probability of tumor eosinophil infiltration versus the probability of type 2 immune cell tumor enrichment in human metastatic melanoma samples (n = 367 tumors). Tumor-infiltration probabilities were determined as described in the Material and Methods using machine learning. The correlation was assessed by using Pearson’s correlation test. Each dot represents one human sample b, Kaplan-Meier overall survival curves of metastatic melanomas (n = 367) plotted against to the likelihood of the high and low infiltration of tumors by type 2 immune cells and eosinophils. Kaplan-Meier survival statistical analysis was performed using a Log-rank test. p-value is indicated. c, Pearson’s correlation analyses of the tumor-infiltrating ILC2 and eosinophil probabilities with CSF2, IL33, IL5, GATA2, RNASE3, EPX, PRG2, TNF and IFNG genes in metastatic tumor melanoma samples available from the publicly TCGA database. Populations and genes that were found to positively and negatively correlate between the indicated populations are shown in red or blue, respectively. Circle size represents the strength of the correlation between two populations.