Extended Data Fig. 8: Genetic inhibition of IL-23 limits resistance to castration in prostate cancer in PtenPC−/− mice. | Nature

Extended Data Fig. 8: Genetic inhibition of IL-23 limits resistance to castration in prostate cancer in PtenPC−/− mice.

From: IL-23 secreted by myeloid cells drives castration-resistant prostate cancer

Extended Data Fig. 8

a, Experimental set-up. Sham-operated (sham) or castrated (CTX) PtenPC−/− mice were lethally irradiated and transplanted with bone marrow precursors depleted of T, B and NK cells from Il23aWT and Il23aKO mice. The mice were then monitored by MRI for tumour progression. b, Representative dot plot of bone marrow precursors pre- and post-depletion of T, B, and NK cells. Data were validated in two biological independent experiments. c, Quantification of the tumour size of sham-operated PtenPC−/−Il23aWT (n = 4) and PtenPC−/−Il23aKO (n = 4), and castrated PtenPC−/−Il23aWT (n = 4) and PtenPC−/−Il23aKO (n = 7) mice at the completion of the study is reported as fold increase of the prostate anterior lobe (AL) volume (fold change compared to the CTX PtenPC−/−Il23aWT group). Data are mean ± s.e.m. Statistical analyses (unpaired two-sided Student’s t-test): **P < 0.01, ***P < 0.001. d, Haematoxylin and eosin, Ki-67 and pSTAT3(Y705) immunohistochemical staining (Ki-67 and pSTAT3(Y705), brown; nuclei, blue) of one representative PtenPC−/−Il23aWT and PtenPC−/−Il23aKO mouse of at least three mice analysed at completion of the study. Scale bars, 50 μm (left) and 25 μm (right).

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