Extended Data Figure 8: Spatial relationships between activated CD4⁺-TLs and lectin⁺ tumour-associated endothelial cells.

a, Schematic of the experimental design. b, A table showing the counts of naive CD4⁺-TLs (tdRed⁺CFSE⁺) and activated CD4⁺-TLs (tdRed⁺) in whole cross sectional area of five animals (n = 5). c, The violin plots showing the kernel probability density of the distances of naive and activated CD4⁺-TLs to the nearest lectin⁺ endothelial cells. Smaller dots without an outline are distances of individual CD4⁺-TL, and larger circles that are outlined represent mean distances taken over all CD4⁺-TLs in the section from the same mouse. CD4⁺-TLs from the same mouse are denoted with the same colour. The P value was calculated using one sample Student’s t-test by comparing the mean distances of activated CD4⁺-TLs from individual mouse with the mean distance of all naive CD4⁺-TLs (dashed horizontal line) (n = 5 mice). d, Top, mosaic scanning images of whole tumour sections. Representative areas are magnified and naive CD4⁺-TLs (yellow) are pinpointed with arrowhead. Bottom, solid lines show the distribution of distances between CD4⁺-TLs and lectin⁺ endothelial cells in whole tumour sections. The mean distances observed are shown as a vertical straight line. For comparison, dashed lines show the probability distribution of mean distances between endothelial cells and computer-simulated random dots. P values were calculated using a permutation-based approach. More detailed information about image simulation is described in the Methods.