Extended Data Figure 4: Validation of representative YTHDF2 RNA targets.

a–d, Examples of transcripts harbouring m⁶A peaks and YTHDF2 PAR-CLIP peaks: SON (CDS, a), CREBBP (3′ UTR, b), LDLR (3′ UTR, c), PLAC2 (non-coding RNA, d). Coverage of m⁶A immunoprecipitation and input fragments are indicated in red and blue, respectively. YTHDF2 PAR-CLIP peaks are highlighted in green. Black lines signify CDS borders. e–n, relative RNA level quantified by gene-specific RT–PCR, and error bars shown in these figure panels are mean ± s.d., n = 6 (two biological replicates × three technical replicates). e, Enrichment fold of SON, CREBBP mRNA, and PLAC2 RNA in YTHDF2-RNA coimmunoprecipitation versus RNA–protein input control, and in m⁶A in vitro immunoprecipitation versus mRNA input control. f, Relative changes of SON, CREBBP mRNA, and PLAC2 RNA in siYTHDF2 sample versus siControl, and overexpression of YTHDF2 versus overexpression of C-YTHDF2. g–k, Lifetimes of SON, CREBBP mRNA and PLAC2 RNA under siYTHDF2 versus siControl. l–n, YTHDF2 knockdown altered the cytoplasmic distribution of its mRNA targets. The SON (l) and CREBBP (m) mRNA levels decreased in the non-ribosome mRNP portion but increased in the 40S–80S portion under siYTHDF2 compared to siControl. However, they showed different changes in the polysome portion. RPL30 (n) is not a target of YTHDF2 and did not show an increase in the 40S–80S portion.