Figure 2
The Intermediate M phenotype is hallmarked by higher N-cadherin and ZEB1 and lower ERBB3 expressions. (a) Plot of QPCR expressions (2^- Avg ΔCt) of CDH2, ITGA5, MMP2 showing the peak expression at Intermediate M (Int M) phenotype. Statistical significance at *P<0.05. (b) IF staining of N-cadherin (N-cad) in Caov3, OVCA432, DOV13 and OVCAR10 representing Epithelial, Intermediate E, Intermediate M and Mesenchymal phenotypes, respectively. Scale bar=200 μm. (c) Distribution of N-cadherin IF positivity among four phenotypes in number (N) and percentage (%). (d) Plot of QPCR expressions (2^- Avg ΔCt) of v-erb-b2 erythroblastic leukaemia viral oncogene homologue 3 (ERBB3) showed lowest expression in the Intermediate M phenotype. Statistical significance at **P<0.05 at both ANOVA and Kruskal–Wallis test. (e) ELISA (f) Plot of QPCR expressions (2^- Avg ΔCt) of snail homologue 1 (SNAI1), zinc-finger E-box-binding homeobox 1 (ZEB1), twist homologue 1 (TWIST1) and zinc-finger E-box-binding homeobox 2 (ZEB2) showing differential expression peaks in Intermediate E (Int E), Intermediate M (Int M) and Mesenchymal (M) phenotypes, respectively. Statistical significance at **P<0.05 in both ANOVA and Kruskal–Wallis tests; Statistical significance at *P<0.05 in either ANOVA or Kruskal–Wallis test