Figure 1
From: A ligand-receptor pair that triggers a non-apoptotic form of programmed cell death
Substance P induces death of neurons in primary culture. (A,B), cortical cells; (C,D), hippocampal cells; (E,F), striatal cells. (A,C,E) are control cultures; (B,D,F) are cells grown in the presence of 100 μM SP for 48 h. Scale bar, 50 μm. G, decreased viability at 48 h in the presence of the indicated concentrations of L-SP in cortical, hippocampal or striatal neurons, or D-SP in cortical cells. Bars represent standard deviations (n=3). (H), SP triggers neuronal cell death at sub-micromolar concentrations. Striatal cells were grown in the presence of the indicated concentrations of SP for 7 days (SP was added only once at the beginning of the experiment). Results from a representative experiment are shown. The experiment was repeated three times with similar results