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26 pages, 6806 KiB  
Article
Physicochemical Properties of Nanoencapsulated Essential Oils: Optimizing D-Limonene Preservation
by Diner Mori-Mestanza, Iraida Valqui-Rojas, Aline C. Caetano, Carlos Culqui-Arce, Rosita Cruz-Lacerna, Ilse S. Cayo-Colca, Efraín M. Castro-Alayo and César R. Balcázar-Zumaeta
Polymers 2025, 17(3), 348; https://doi.org/10.3390/polym17030348 - 27 Jan 2025
Abstract
Essential oils exhibit antioxidant properties but are prone to oxidative degradation under environmental conditions, making their preservation crucial. Therefore, the purpose of this work was to evaluate the physicochemical properties of nanoencapsulated essential oils (EOs) extracted from the peel of sweet lemon, mandarin, [...] Read more.
Essential oils exhibit antioxidant properties but are prone to oxidative degradation under environmental conditions, making their preservation crucial. Therefore, the purpose of this work was to evaluate the physicochemical properties of nanoencapsulated essential oils (EOs) extracted from the peel of sweet lemon, mandarin, lime, and orange using four formulations of wall materials consisting of gum arabic (GA), maltodextrin (MD), and casein (CAS). The results showed that EOs from sweet lemon, mandarin, lime, and orange showed higher solubility (79.5% to 93.5%) when encapsulated with GA/MD. Likewise, EOs from sweet lemon showed the highest phenolic content when using GA/CAS (228.27 mg GAE/g sample), and the encapsulated EOs of sweet lemon and mandarin with GA/MD/CAS (1709 and 1599 μmol TE/g) had higher antioxidant capacity. On the other hand, higher encapsulation efficiency was obtained in EOs of lime encapsulated with GA/MD (68.5%), and the nanoencapsulates of EOs from sweet lemon with GA/MD had higher D-limonene content (613 ng/mL). Using gum arabic and maltodextrin increased the encapsulation efficiency and D-limonene content in EO of sweet lemon. On the other hand, the formulations with casein were the most efficient wall materials for retaining D-limonene from the EOs of mandarin, lime, and orange. Full article
(This article belongs to the Special Issue Biopolymer Matrices for Incorporation of Bioactive Compounds)
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Figure 1

Figure 1
<p>Solid yield of nanoparticles of (<b>A</b>) sweet lemon (<span class="html-italic">C</span>. <span class="html-italic">limetta</span> sp.), (<b>B</b>) mandarin (<span class="html-italic">C. reticulata</span>), (<b>C</b>) lime (<span class="html-italic">C. limetta</span> Risso), (<b>D</b>) orange (<span class="html-italic">C. sinensis</span>) EOs encapsulated with four wall materials.</p>
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<p>Chemical compounds found in (<b>A</b>) Sweet lemon (<span class="html-italic">C. limetta</span> sp.), (<b>B</b>) mandarin (<span class="html-italic">C. reticulata</span>), (<b>C</b>) lime (<span class="html-italic">C. limetta</span> Risso), and (<b>D</b>) orange (<span class="html-italic">C. sinensis</span>) EOs nanoencapsulated with four wall material.</p>
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<p>D-limonene concentration in (<b>A</b>) Sweet lemon (<span class="html-italic">C. limetta</span> sp.), (<b>B</b>) mandarin (<span class="html-italic">C. reticulata</span>), (<b>C</b>) lime (<span class="html-italic">C. limetta</span> Risso), and (<b>D</b>) orange (<span class="html-italic">C. sinensis</span>) EOs nanoencapsulated with four wall material. Different lower case letters in the graphs indicate significant differences (<span class="html-italic">p</span> ≤ 0.05).</p>
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<p>Scanning electron microscopy (SEM) of nanocapsules of EOs of <span class="html-italic">C. limetta</span> Risso with MD/GA (<b>A</b>), GA/CAS (<b>B</b>), MD/CAS (<b>C</b>), or MD/GA/CAS (<b>D</b>).</p>
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<p>Scanning electron microscopy (SEM) of nanocapsules of EOs of <span class="html-italic">C. sinensis</span> with MD/GA (<b>A</b>), GA/CAS (<b>B</b>), MD/CAS (<b>C</b>), or MD/GA/CAS (<b>D</b>).</p>
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<p>Scanning electron microscopy (SEM) of nanocapsules of EOs of <span class="html-italic">C. limetta</span> sp. with MD/GA (<b>A</b>), GA/CAS (<b>B</b>), MD/CAS (<b>C</b>), or MD/GA/CAS (<b>D</b>).</p>
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<p>Scanning electron microscopy (SEM) of nanocapsules of EOs of <span class="html-italic">C. reticulata</span> with MD/GA (<b>A</b>), GA/CAS (<b>B</b>), MD/CAS (<b>C</b>) or MD/GA/CAS (<b>D</b>).</p>
Full article ">Figure 8
<p>ATR-FT-IR spectra of nanoencapsulated essential oils of Sweet lemon (<span class="html-italic">C. limetta</span> sp.), mandarin (<span class="html-italic">C. reticulata</span>), lime (<span class="html-italic">C. limetta Risso</span>), and orange (<span class="html-italic">C. sinensis</span>) with four wall material: (<b>A</b>) GA/CAS, (<b>B</b>) MD/CAS, (<b>C</b>) MD/GA and (<b>D</b>) GA/MD/CAS.</p>
Full article ">Figure 8 Cont.
<p>ATR-FT-IR spectra of nanoencapsulated essential oils of Sweet lemon (<span class="html-italic">C. limetta</span> sp.), mandarin (<span class="html-italic">C. reticulata</span>), lime (<span class="html-italic">C. limetta Risso</span>), and orange (<span class="html-italic">C. sinensis</span>) with four wall material: (<b>A</b>) GA/CAS, (<b>B</b>) MD/CAS, (<b>C</b>) MD/GA and (<b>D</b>) GA/MD/CAS.</p>
Full article ">
14 pages, 706 KiB  
Article
Assessment of the Conditions of Anchor Bolts Grouted with Resin and Cement Through Impact-Echo Testing and Advanced Spectrum Analysis
by Wael Zatar, Feng Xiao, Gang Chen and Hien Nghiem
Buildings 2025, 15(3), 399; https://doi.org/10.3390/buildings15030399 - 26 Jan 2025
Abstract
Anchor bolts, such as rock bolts and concrete anchors, are widely used in civil, geotechnical, and mining engineering for anchorage and ground support. They are used in retaining walls, dry docks, dams, mines, and prestressed concrete structures. Evaluating the grouting condition of anchor [...] Read more.
Anchor bolts, such as rock bolts and concrete anchors, are widely used in civil, geotechnical, and mining engineering for anchorage and ground support. They are used in retaining walls, dry docks, dams, mines, and prestressed concrete structures. Evaluating the grouting condition of anchor bolts is essential to ensure the safety of these applications. Spectrum techniques have been used to develop non-destructive methods for estimating the grouting quality of grouted anchor bolts. The spectrum methods include fast Fourier transform, time–frequency analysis, wavelet transform analysis, and empirical mode decomposition. In this study, we introduce the parameter-optimized variational mode decomposition (VMD) method for the spectrum analysis of impact echo signals of anchor bolts. This method overcomes the difficulty of conventional spectrum methods that cannot separate highly coupled natural modes for advanced analysis. The parameter-optimized VMD method enables the generation of a new evaluation index for quantifying bolt grouting conditions, which has the potential to significantly enhance the quality evaluation of anchor bolts compared with conventional analysis of natural frequencies. This study uses impact response to establish a new benchmark for the integrity diagnosis of anchor bolts, paving the way for more accurate and reliable safety assessments. Full article
15 pages, 11371 KiB  
Article
Effect of Grouting, Concrete Cover, and Combined Reinforcement on Masonry Retaining Walls
by Wei Cheng, Cong Zhu, Gongzuo Shi, Ze Liu, Cheng Liu, Yinguang Du, Yu Chen, Changchun Zhuang and Hongqiang Gu
Buildings 2025, 15(3), 309; https://doi.org/10.3390/buildings15030309 - 21 Jan 2025
Viewed by 281
Abstract
Masonry retaining walls used in civil engineering projects, such as highway embankments and slope protections, easily crack due to complex internal pore structures and exposure to harsh environmental conditions. To address these problems, practical reinforcement methods, including grouting reinforcement, concrete cover reinforcement, and [...] Read more.
Masonry retaining walls used in civil engineering projects, such as highway embankments and slope protections, easily crack due to complex internal pore structures and exposure to harsh environmental conditions. To address these problems, practical reinforcement methods, including grouting reinforcement, concrete cover reinforcement, and combined reinforcement, were proposed to maintain retaining walls in this study. Nine cases of different reinforcement schemes were adopted to investigate the effects of grouting volumes, grouting hole numbers, and reinforcement methods. The results showed that as the grouting volume and grouting hole numbers increased, the cracks occurred at a lower height, showing a higher moment resistance capacity. In addition, the cracking moment was enhanced with a thicker concrete cover. Furthermore, combined grouting and concrete cover reinforcement improved the structural integrity and showed the best performance, in which the failure mode shifted from brittle to ductile. However, concrete cover reinforcement is associated with a higher price and longer construction cycle. Thus, decisions should be made depending on the engineering requirement. Full article
(This article belongs to the Section Building Materials, and Repair & Renovation)
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Figure 1

Figure 1
<p>Layout of grouting holes.</p>
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<p>Layout of (<b>a</b>) embedded strain gauges and (<b>b</b>) bonded strain gauges.</p>
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<p>Loading equipment: (<b>a</b>) schematic setup and (<b>b</b>) photograph.</p>
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<p>The displacement of the cable displacement sensors of wall 1.</p>
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<p>The relationship between maximum bending moment and displacement of wall 1.</p>
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<p>The relationship between strain and displacement of wall 1.</p>
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<p>The displacement of cable displacement sensors of grouted reinforced retaining walls under different grouting conditions: (<b>a</b>) wall 2 with 0.90 m<sup>3</sup> grouting volume, (<b>b</b>) wall 3 with 1.25 m<sup>3</sup> grouting volume, (<b>c</b>) wall 4 with two grouting holes, and (<b>d</b>) wall 5 with three grouting holes.</p>
Full article ">Figure 7 Cont.
<p>The displacement of cable displacement sensors of grouted reinforced retaining walls under different grouting conditions: (<b>a</b>) wall 2 with 0.90 m<sup>3</sup> grouting volume, (<b>b</b>) wall 3 with 1.25 m<sup>3</sup> grouting volume, (<b>c</b>) wall 4 with two grouting holes, and (<b>d</b>) wall 5 with three grouting holes.</p>
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<p>The relationship between maximum bending moment and displacement of grouted reinforced retaining walls under different grouting conditions: (<b>a</b>) wall 2 with 0.90 m<sup>3</sup> grouting volume, (<b>b</b>) wall 3 with 1.25 m<sup>3</sup> grouting volume, (<b>c</b>) wall 4 with two grouting holes, and (<b>d</b>) wall 5 with three grouting holes.</p>
Full article ">Figure 8 Cont.
<p>The relationship between maximum bending moment and displacement of grouted reinforced retaining walls under different grouting conditions: (<b>a</b>) wall 2 with 0.90 m<sup>3</sup> grouting volume, (<b>b</b>) wall 3 with 1.25 m<sup>3</sup> grouting volume, (<b>c</b>) wall 4 with two grouting holes, and (<b>d</b>) wall 5 with three grouting holes.</p>
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<p>The relationship between strain and displacement: (<b>a</b>) wall 2 with 0.90 m<sup>3</sup> grouting volume, (<b>b</b>) wall 3 with 1.25 m<sup>3</sup> grouting volume, (<b>c</b>) wall 4 with two grouting holes, and (<b>d</b>) wall 5 with three grouting holes.</p>
Full article ">Figure 9 Cont.
<p>The relationship between strain and displacement: (<b>a</b>) wall 2 with 0.90 m<sup>3</sup> grouting volume, (<b>b</b>) wall 3 with 1.25 m<sup>3</sup> grouting volume, (<b>c</b>) wall 4 with two grouting holes, and (<b>d</b>) wall 5 with three grouting holes.</p>
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<p>The displacement of cable displacement sensors of reinforced retaining walls with concrete cover: (<b>a</b>) wall 6 with 10 cm concrete cover and (<b>b</b>) wall 7 with 20 cm concrete cover.</p>
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<p>The relationship between maximum bending moment and displacement of reinforced retaining walls with concrete cover: (<b>a</b>) wall 6 with 10 cm concrete cover, (<b>b</b>) wall 7 with 20 cm concrete cover.</p>
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<p>The relationship between maximum bending moment and strain of (<b>a</b>) wall 6, (<b>b</b>) wall 7, (<b>c</b>) concrete cover of wall 6, and (<b>d</b>) concrete cover of wall 7.</p>
Full article ">Figure 12 Cont.
<p>The relationship between maximum bending moment and strain of (<b>a</b>) wall 6, (<b>b</b>) wall 7, (<b>c</b>) concrete cover of wall 6, and (<b>d</b>) concrete cover of wall 7.</p>
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<p>The displacement of cable displacement sensors of composite reinforced retaining walls: (<b>a</b>) wall 8 without steel grouting pipes and (<b>b</b>) wall 9 with steel grouting pipes.</p>
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<p>The relationship between maximum bending moment and displacement of composite reinforced retaining walls: (<b>a</b>) wall 8 without steel grouting pipes and (<b>b</b>) wall 9 with steel grouting pipes.</p>
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<p>The relationship between maximum bending moment and strain of (<b>a</b>) wall 8, (<b>b</b>) wall 9, (<b>c</b>) concrete cover of wall 8, and (<b>d</b>) concrete cover of wall 9.</p>
Full article ">Figure 15 Cont.
<p>The relationship between maximum bending moment and strain of (<b>a</b>) wall 8, (<b>b</b>) wall 9, (<b>c</b>) concrete cover of wall 8, and (<b>d</b>) concrete cover of wall 9.</p>
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18 pages, 12789 KiB  
Article
A Study on the Residual Oil Distribution in Tight Reservoirs Based on a 3D Pore Structure Model
by Rujun Wang, Yintao Zhang, Chong Sun, Jing Li, Xiaoyu Meng, Chengqiang Yang and Zhaoyang Chen
Processes 2025, 13(1), 203; https://doi.org/10.3390/pr13010203 - 13 Jan 2025
Viewed by 434
Abstract
A tight reservoir is characterized by low porosity and permeability as well as a complex pore structure, resulting in low oil recovery efficiency. Understanding the micro-scale distribution of residual oil is of great significance for improving oil production and water flooding recovery rates. [...] Read more.
A tight reservoir is characterized by low porosity and permeability as well as a complex pore structure, resulting in low oil recovery efficiency. Understanding the micro-scale distribution of residual oil is of great significance for improving oil production and water flooding recovery rates. In this study, a 3D pore structure model of tight sandstone was established using CT scanning to characterize the residual oil distribution after water flooding. The effects of displacement methods and wettability on residual oil distribution at the micro-scale were then studied and discussed. Moreover, increasing the displacement rate has little effect on the distribution area and dominant seepage channels. Microscopic residual oil is classified into five discontinuous phases according to the oil–water–pore–throat contact relationship. The microscopic residual oil exhibits characteristics of being dispersed overall but locally concentrated. Under water-wet conditions, the injected water tends to strip the oil phase along the pore walls. Under oil-wet conditions, the pore walls have an improved adsorption capacity for the oil phase, resulting in a large amount of porous and membranous residual oil retained in the pores, which leads to a decrease in the overall recovery rate. Full article
(This article belongs to the Topic Exploitation and Underground Storage of Oil and Gas)
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Figure 1

Figure 1
<p>Gray scale image processing: (<b>a</b>) reginal grayscale image; (<b>b</b>) gray scale value optimization; (<b>c</b>) threshold segmentation; (<b>d</b>) median filtering.</p>
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<p>Three-Dimensional digital core model composition: (<b>a</b>) complete digital cores; (<b>b</b>) internal structure of rocks; (<b>c</b>) isolated pores and microcracks; (<b>d</b>) connected pores.</p>
Full article ">Figure 2 Cont.
<p>Three-Dimensional digital core model composition: (<b>a</b>) complete digital cores; (<b>b</b>) internal structure of rocks; (<b>c</b>) isolated pores and microcracks; (<b>d</b>) connected pores.</p>
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<p>Finite element modeling of M1 and M2.</p>
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<p>Boundary condition of M1.</p>
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<p>Microscopic residual oil distribution patterns: (<b>a</b>) porous residual oil; (<b>b</b>) droplets residual oil; (<b>c</b>) membranous residual oil; (<b>d</b>) corner residual oil; (<b>e</b>) columnar residual oil.</p>
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<p>Residual oil in M1 (pore) and M2 (pore-crack): (<b>a</b>) M1 model; (<b>b</b>) M2 model.</p>
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<p>Oil-water phases saturations at various wetting angles under water-wet conditions.</p>
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<p>Oil–water phase saturations at different wetting angles under oil-wet conditions.</p>
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<p>Oil–water phase distribution at different displacement velocities.</p>
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<p>The degree of residual oil extraction varies with different displacement velocities: (<b>a</b>) M1 model; (<b>b</b>) M2 model.</p>
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<p>The distribution of oil–water phases changes with varying displacement directions: (<b>a</b>) vertically displacement alternation; (<b>b</b>) change in direction of displacement by 90° (lateral displacement); (<b>c</b>) change in direction of displacement by 180° (reverse displacement); (<b>d</b>) change in direction of displacement by 270° (lateral displacement).</p>
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<p>The fluid flow of M1 model under different displacement directions: (<b>a</b>) reverse displace (180°); (<b>b</b>) lateral displace (90°); (<b>c</b>) lateral displace (270°).</p>
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<p>The residual oil extraction degree with different water displacement directions.</p>
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19 pages, 4490 KiB  
Article
Optimizing Non-Thermal Magnetic Field to Minimize Weight Loss and Tissue Degradation: Identifying Possible Enzyme Inhibition Mechanisms
by Chao-Kai Chang, Prakoso Adi, Rizka Mulyani, Chun-Fu Lin, Ratna Sari Listyaningrum, Shella Permatasari Santoso, Mohsen Gavahian and Chang-Wei Hsieh
Foods 2025, 14(2), 166; https://doi.org/10.3390/foods14020166 - 8 Jan 2025
Viewed by 553
Abstract
This research investigates potential mechanisms of novel magnetic field (MF) treatments in inhibiting cell-wall-degrading enzymes, aiming to reduce weight loss and preserve the post-harvest quality of tomatoes (Solanum lycopersicum L.) as a climacteric fruit. The optimization of the processing parameters, including MF [...] Read more.
This research investigates potential mechanisms of novel magnetic field (MF) treatments in inhibiting cell-wall-degrading enzymes, aiming to reduce weight loss and preserve the post-harvest quality of tomatoes (Solanum lycopersicum L.) as a climacteric fruit. The optimization of the processing parameters, including MF intensity (1, 2, 3 mT), frequency (0, 50, 100 Hz), and duration (10, 20, 30 min), was accomplished by applying an orthogonal array design. In particular, the investigation delved into the underlying mechanisms by which MF impedes the activity of tissue-degrading enzymes, such as pectin esterase (PE), polygalacturonase (PG), and cellulase (Cx), during the storage period. The results showed that MF treatment delayed the increase in soluble solids by 1.5 times and reduced titratable acidity by 1.2 times. The optimal treatment conditions—2 mT, 50 Hz, and 10 min—achieved the most significant inhibition of weight loss (4.22%) and maintained tissue integrity for up to 21 days. Optimized MF significantly suppressed enzyme activity, with PE activity reduced by 1.5 times, PG by 2.8 times, and Cx by 2.5 times. Also, cross-sectional images and external appearance demonstrated that MF-treated tomatoes retained their internal tissue structure throughout the extended storage period. These findings suggest that MF treatments can effectively suppress the key enzymes responsible for tissue degradation, ultimately delaying weight loss and softening, preserving post-harvest quality, and contributing to sustainable food production and zero waste. Full article
(This article belongs to the Section Food Engineering and Technology)
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Graphical abstract

Graphical abstract
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<p>Schematic representation of the MF treatment system.</p>
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<p>Analysis of the orthogonal array design (OAD) for (<b>A</b>) signal-to-noise (SN) ratio and (<b>B</b>) means of the impact of the optimization factors on the weight loss of tomatoes.</p>
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<p>Effect of optimal MF parameters on the (<b>A</b>) appearance, (<b>B</b>) weight loss (%), (<b>C</b>) organic acid content (%), and (<b>D</b>) soluble solid content (°Brix) of tomatoes during storage for 21 days at 25 °C (<span class="html-italic">n</span> = 10). The MF group was treated with an MF strength of 2 mT, a frequency of 50 Hz, and a treatment time of 10 min; the untreated control group was stored in an environment at 25 °C for 21 days. Letters a and b indicate statistical differences (<span class="html-italic">p</span> &lt; 0.05) using unpaired <span class="html-italic">t</span>-test.</p>
Full article ">Figure 3 Cont.
<p>Effect of optimal MF parameters on the (<b>A</b>) appearance, (<b>B</b>) weight loss (%), (<b>C</b>) organic acid content (%), and (<b>D</b>) soluble solid content (°Brix) of tomatoes during storage for 21 days at 25 °C (<span class="html-italic">n</span> = 10). The MF group was treated with an MF strength of 2 mT, a frequency of 50 Hz, and a treatment time of 10 min; the untreated control group was stored in an environment at 25 °C for 21 days. Letters a and b indicate statistical differences (<span class="html-italic">p</span> &lt; 0.05) using unpaired <span class="html-italic">t</span>-test.</p>
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<p>Effect of the optimal MF on the tissue deterioration enzymes (<b>A</b>) PE, (<b>B</b>) PG, and (<b>C</b>) CX of tomatoes during storage for 21 days at 25 °C (<span class="html-italic">n</span> = 10). The MF group was treated with an MF strength of 2 mT, frequency of 50 Hz, and treatment time of 10 min; the untreated control group was stored in an environment at 25 °C for 21 days. Letters a and b indicate statistical differences (<span class="html-italic">p</span> &lt; 0.05) using unpaired <span class="html-italic">t</span>-test.</p>
Full article ">Figure 5
<p>Double reciprocal plot of the activity of the tomato tissue deterioration enzymes (<b>A</b>) PE, (<b>B</b>) PG, and (<b>C</b>) Cx after optimal MF treatment. V<sub>max</sub> is the maximum velocity of the reaction, and K<sub>m</sub> is the concentration of the substrate that permits the enzyme to achieve half V<sub>max</sub>.</p>
Full article ">Figure 5 Cont.
<p>Double reciprocal plot of the activity of the tomato tissue deterioration enzymes (<b>A</b>) PE, (<b>B</b>) PG, and (<b>C</b>) Cx after optimal MF treatment. V<sub>max</sub> is the maximum velocity of the reaction, and K<sub>m</sub> is the concentration of the substrate that permits the enzyme to achieve half V<sub>max</sub>.</p>
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<p>The proposed mechanism of the MF inhibits tomato tissue degradation enzymes (PE, PG, and Cx).</p>
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26 pages, 7027 KiB  
Article
Parametric CFD Study of Spray Drying Chamber Geometry: Part I—Effects on Airflow Dynamics
by Jairo Andrés Gutiérrez Suárez, Carlos Humberto Galeano Urueña and Alexánder Gómez Mejía
ChemEngineering 2025, 9(1), 5; https://doi.org/10.3390/chemengineering9010005 - 4 Jan 2025
Viewed by 658
Abstract
Internal airflow dynamics play a crucial role in spray drying engineering by governing particle transport and, consequently, the quality of dried products. For this application, airflow dynamics represent short- and long-timescale behaviors across the main jet and recirculation regions and have been related, [...] Read more.
Internal airflow dynamics play a crucial role in spray drying engineering by governing particle transport and, consequently, the quality of dried products. For this application, airflow dynamics represent short- and long-timescale behaviors across the main jet and recirculation regions and have been related, among other factors, to spray chamber design. This study examines the parametric effects of key geometrical design parameters on internal airflow dynamics using Design of Experiments (DOE) methodologies and 3D Computational Fluid Dynamics (CFD) simulations. The CFD model adopts a cost-efficient approach, including adaptive mesh refinement (AMR) methods, enabling running multiple simulation cases while retaining turbulence-resolving capabilities. The results provide quantitative parameter–response relationships, offering insights into the impact of chamber geometry on complex airflow behaviors. Among the parameters studied, the chamber aspect ratio strongly influences the strength of external recirculation flows. The inlet swirl primarily governs the stability of central and recirculating flows, while the conical–cylindrical section topology, in conjunction with the jet Reynolds number, affects flow impingement on walls, predominantly caused by the precession and reversal of the central jet. This methodology demonstrates significant potential for future studies on particle drying, equipment, process scale-up, and alternative chamber configurations in spray drying systems. Full article
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Figure 1

Figure 1
<p>Parametrized geometry with factors and fixed parameters used in the DOE study.</p>
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<p>Measurement regions inside a normalized drying chamber, including the jet discharge (R-0), central jet (R-1), and external recirculation (R-2) regions. Details of R-1 and R-2 are provided in <a href="#ChemEngineering-09-00005-t003" class="html-table">Table 3</a>.</p>
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<p>Computational grids for Experiments 5 and 13 (<b>top</b>) and Experiments 8 and 16 (<b>bottom</b>), including mesh details around the jet discharge and upper recirculation region.</p>
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<p>Unsteady flow and dynamic mesh behavior for Experiment 8. (<b>Top</b>): A 2D-cross section of the drying chamber showing velocity contours at different characteristic times. (<b>Bottom</b>): Mesh adaptation behavior at different characteristic times.</p>
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<p>Some examples of sampled data for Experiments 3 and 15; (<b>a</b>) measured <math display="inline"><semantics> <msub> <mi>U</mi> <mi>x</mi> </msub> </semantics></math> over the axis at <math display="inline"><semantics> <mrow> <mi>x</mi> <mo>/</mo> <mi>H</mi> <mo>=</mo> <mn>0.5</mn> </mrow> </semantics></math>; (<b>b</b>) 2D velocity vectors obtained by averaging the recirculation zone data (R-2); (<b>c</b>) FFT of the <math display="inline"><semantics> <msub> <mi>U</mi> <mi>x</mi> </msub> </semantics></math> data presented in a).</p>
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<p>Factor effects analysis of the influence of factor parameters on the response variables. <b>Left</b> column: main effects plot showing the mean response value for each level of the two-level factor; <b>right</b> column: Pareto chart of the standardized main effects. The horizontal blue line defines the 95% confidence interval (<span class="html-italic">p</span> = 0.05). The value of <math display="inline"><semantics> <mi>α</mi> </semantics></math> represents the threshold value in the response variable for this confidence interval. <math display="inline"><semantics> <mrow> <mi>S</mi> <msub> <mi>S</mi> <mi>T</mi> </msub> </mrow> </semantics></math> represents the sum of the squares.</p>
Full article ">Figure 6 Cont.
<p>Factor effects analysis of the influence of factor parameters on the response variables. <b>Left</b> column: main effects plot showing the mean response value for each level of the two-level factor; <b>right</b> column: Pareto chart of the standardized main effects. The horizontal blue line defines the 95% confidence interval (<span class="html-italic">p</span> = 0.05). The value of <math display="inline"><semantics> <mi>α</mi> </semantics></math> represents the threshold value in the response variable for this confidence interval. <math display="inline"><semantics> <mrow> <mi>S</mi> <msub> <mi>S</mi> <mi>T</mi> </msub> </mrow> </semantics></math> represents the sum of the squares.</p>
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<p>Factor effects analysis of the influence of factor parameters on the response variables. <b>Left</b> column: main effects plot showing the mean response value for each level of the two-level factor; <b>right</b> column: Pareto chart of the standardized main effects. The horizontal blue line defines the 95% confidence interval (<span class="html-italic">p</span> = 0.05). The value of <math display="inline"><semantics> <mi>α</mi> </semantics></math> represents the threshold value in the response variable for this confidence interval. <math display="inline"><semantics> <mrow> <mi>S</mi> <msub> <mi>S</mi> <mi>T</mi> </msub> </mrow> </semantics></math> represents the sum of the squares.</p>
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<p>Main effects plot including center point results (Experiment 17) in the main jet (R-1) and the recirculation region (R-2).</p>
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24 pages, 5273 KiB  
Article
Design Optimization of an Innovative Instrumental Single-Sided Formwork Supporting System for Retaining Walls Using Physics-Constrained Generative Adversarial Network
by Wei Liu, Lin He, Jikai Liu, Xiangyang Xie, Ning Hao, Cheng Shen and Junyong Zhou
Buildings 2025, 15(1), 132; https://doi.org/10.3390/buildings15010132 - 4 Jan 2025
Viewed by 634
Abstract
Single-sided formwork supporting systems (SFSSs) play a crucial role in the urban construction of retaining walls using cast-in-place concrete. By supporting the formwork from one side, an SFSS can minimize its spatial footprint, enabling its closer placement to boundary lines without compromising structural [...] Read more.
Single-sided formwork supporting systems (SFSSs) play a crucial role in the urban construction of retaining walls using cast-in-place concrete. By supporting the formwork from one side, an SFSS can minimize its spatial footprint, enabling its closer placement to boundary lines without compromising structural integrity. However, existing SFSS designs struggle to achieve a balance between mechanical performance and lightweight construction. To address these limitations, an innovative instrumented SFSS was proposed. It is composed of a panel structure made of a panel, vertical braces, and cross braces and a supporting structure comprising an L-shaped frame, steel tubes, and anchor bolts. These components are conducive to modular manufacturing, lightweight installation, and convenient connections. To facilitate the optimal design of this instrumented SFSS, a physics-constrained generative adversarial network (PC-GAN) approach was proposed. This approach incorporates three objective functions: minimizing material usage, adhering to deformation criteria, and ensuring structural safety. An example application is presented to demonstrate the superiority of the instrumented SFSS and validate the proposed PC-GAN approach. The instrumented SFSS enables individual components to be easily and rapidly prefabricated, assembled, and disassembled, requiring only two workers for installation or removal without the need for additional hoisting equipment. The optimized instrumented SFSS, designed using the PC-GAN approach, achieves comparable deformation performance (from 2.49 mm to 2.48 mm in maxima) and slightly improved component stress levels (from 97 MPa to 115 MPa in maxima) while reducing the total weight by 20.85%, through optimizing panel thickness, the dimensions and spacings of vertical and lateral braces, and the spacings of steel tubes. This optimized design of the instrumented SFSS using PC-GAN shows better performance than the current scheme, combining significant weight reduction with enhanced mechanical efficiency. Full article
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<p>Arrangement and construction diagram of the tie rod SFSS.</p>
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<p>Typical structural forms of triangular truss SFSS.</p>
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<p>Typical design scheme of steel pipe SFSS.</p>
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<p>Structural layout and constituent parts of the instrumental SFSS.</p>
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<p>Mechanical schematic diagram of the overall structure of the instrumental SFSS.</p>
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<p>Mechanical schematic diagram of the panel structure.</p>
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<p>Mechanical schematic diagram of the triangular supporting structure.</p>
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<p>Flowchart of the PC-GAN approach for design optimization of the SFSS.</p>
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<p>Photos of SFSS assembly construction and completion of concrete wall construction.</p>
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<p>Deformation of the instrumental SFSS of the initial design.</p>
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<p>Stress distribution of the instrumental SFSS of the initial design. (<b>a</b>) The laminated timber panel structure; (<b>b</b>) the steel supporting structure.</p>
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<p>Iterations of the PC-GAN approach and distributions of generated parameter values.</p>
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<p>Deformation of the instrumental SFSS after optimized design.</p>
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<p>Stress distribution of the instrumental SFSS after optimized design. (<b>a</b>) The laminated timber panel structure; (<b>b</b>) the steel supporting structure.</p>
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18 pages, 4027 KiB  
Article
Analysis of the Structural Behavior Evolution of Reinforced Soil Retaining Walls Under the Combined Effects of Rainfall and Earthquake
by Xinxin Li, Xiaoguang Cai, Sihan Li, Xin Huang, Chen Zhu and Honglu Xu
Buildings 2025, 15(1), 115; https://doi.org/10.3390/buildings15010115 - 31 Dec 2024
Viewed by 644
Abstract
Major earthquakes and rainfall may occur at the same time, necessitating further investigation into the dynamic characteristics and responses of reinforced soil retaining walls subjected to the combined forces of rainfall and seismic activity. Three sets of shaking table tests on model retaining [...] Read more.
Major earthquakes and rainfall may occur at the same time, necessitating further investigation into the dynamic characteristics and responses of reinforced soil retaining walls subjected to the combined forces of rainfall and seismic activity. Three sets of shaking table tests on model retaining walls were designed, a modular reinforced earth retaining wall was utilized as the subject of this study, and a custom-made device was made to simulate rainfall conditions of varying intensities. These tests monitored the rainwater infiltration pattern, macroscopic phenomena, panel displacement, tension behavior, dynamic characteristics, and acceleration response of the modular reinforced earth retaining wall during vibration under different rainfall intensities. The results indicated the following. (1) Rainwater infiltration can be categorized into three stages: rapid rise, rapid decline, and slow decline to stability. The duration for infiltration to reach stability increases with greater rainfall. (2) An increase in rainfall intensity enhances the seismic stability of the retaining wall panel, as higher rainfall intensity results in reduced sand leakage from the panel, thereby diminishing panel deformation during vibration. (3) Increased rainfall intensity decreases the shear strength of the soil, leading to a greater load on the reinforcement. (4) The natural vibration frequencies of the three groups of retaining walls decreased by 0.21%, 0.54%, and 2.326%, respectively, indicating some internal damage within the retaining walls, although the degree of damage was not severe. Additionally, the peak displacement of the panel increased by 0.91 mm, 0.63 mm, and 0.61 mm, respectively. (5) The amplification effect of rainfall on internal soil acceleration is diminished, with this weakening effect becoming more pronounced as rainfall intensity increases. These research findings can provide a valuable reference for multi-disaster risk assessments of modular reinforced soil retaining walls. Full article
(This article belongs to the Section Building Structures)
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<p>Grain size distribution curve.</p>
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<p>Connection mode between reinforcement and panel.</p>
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<p>Rainfall device.</p>
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<p>Flow diagram of different pump tube speeds.</p>
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<p>Instrument layout.</p>
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<p>Time history of El Centro ground motion.</p>
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<p>Time history of white noise.</p>
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<p>Wetting front during and after rainfall: (<b>a</b>) R1; (<b>b</b>) R2; (<b>c</b>) R3.</p>
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<p>Wetting front during and after rainfall: (<b>a</b>) R1; (<b>b</b>) R2; (<b>c</b>) R3.</p>
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<p>Time history curve of moisture content: (<b>a</b>) R1; (<b>b</b>) R2; (<b>c</b>) R3.</p>
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<p>Panel sand leakage during vibration: (<b>a</b>) R1; (<b>b</b>) R2, (<b>c</b>) R3.</p>
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<p>Top crack after vibration.</p>
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<p>Deformation trend of panel.</p>
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<p>(<b>a</b>) Panel deformation before vibration; (<b>b</b>) panel deformation after vibration.</p>
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<p>Tension distribution of reinforcement: (<b>a</b>) R1; (<b>b</b>) R2; (<b>c</b>) R3.</p>
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<p>Distribution of natural frequency and damping ratio: (<b>a</b>) R1; (<b>b</b>) R2; (<b>c</b>) R3.</p>
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<p>Comparison of natural frequency and damping ratio distribution: (<b>a</b>) comparison of natural frequency distribution; (<b>b</b>) summary comparison of damping ratio distribution.</p>
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<p>Acceleration amplification factor distribution: (<b>a</b>) R1; (<b>b</b>) R2; (<b>c</b>) R3.</p>
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21 pages, 11485 KiB  
Article
Numerical Investigation on Deep-Foundation Pit Excavation Supported by Box-Type Retaining Walls
by Peng Peng, Weiyao Kong, Saishuai Huang, Yi Long and Yang Lu
Buildings 2025, 15(1), 109; https://doi.org/10.3390/buildings15010109 - 31 Dec 2024
Viewed by 443
Abstract
In soft soil foundations, the utilization of box-type retaining walls as a support method represents a novel approach. This study focuses on investigating the key factors influencing lateral wall deflection and ground settlement behind the wall in deep excavation projects supported by box-type [...] Read more.
In soft soil foundations, the utilization of box-type retaining walls as a support method represents a novel approach. This study focuses on investigating the key factors influencing lateral wall deflection and ground settlement behind the wall in deep excavation projects supported by box-type retaining walls. Based on a practical engineering case in Shanghai, the large deformation Lagrangian numerical simulation software FLAC-3D is employed to simulate the displacement of box-type retaining walls as well as the surface settlement surrounding the excavation pit during the excavation process of deep-foundation pits. This research encompasses aspects such as the box size, the filling material within the box, and the constituent materials of the retaining wall. Ultimately, it is concluded that variations in the size of the box-retaining wall have a significant impact on wall deflection and surrounding ground settlement, while the filling material and constituent materials have relatively minor effects. This study provides a theoretical basis and scientific reference for the design and construction of box-type retaining walls in deep-foundation pit engineering. Full article
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<p>Location of the considered project: (<b>a</b>) picture of foundation pit zoning; (<b>b</b>) graphic layout of foundation pit zoning.</p>
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<p>Construction site images: (<b>a</b>) Excavation of pit inside box-type retaining wall; (<b>b</b>) Excavation construction of the foundation pit to the bottom; (<b>c</b>) Completed box-type retaining wall; (<b>d</b>) Completed foundation pit site; (<b>e</b>) Schematic diagram for lateral deformation (inclinometry) monitoring of retaining walls.</p>
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<p>(<b>a</b>) The cross-section of the foundation pit with box-type retaining wall. (<b>b</b>) Soil profiles and material properties at the site.</p>
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<p>(<b>a</b>) The cross-section of the foundation pit with box-type retaining wall. (<b>b</b>) Soil profiles and material properties at the site.</p>
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<p>Numerical simulation model. (<b>a</b>) FLAC 3D calculation model of the whole foundation pit. (<b>b</b>) Model boundary conditions.</p>
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<p>Detailed numerical simulation model. (<b>a</b>) Underground diaphragm wall. (<b>b</b>) Back wall of box-type retaining wall. (<b>c</b>) Three-axis mixing pile. (<b>d</b>) Bored pile.</p>
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<p>Comparison of measured and simulated deformations at measurement location of the front wall of the box-type retaining wall displacement.</p>
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<p>(<b>a</b>) Settlement curve of the foundation pit. (<b>b</b>) The influence zone behind the wall.</p>
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<p>(<b>a</b>) Settlement curve of the foundation pit. (<b>b</b>) The influence zone behind the wall.</p>
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<p>Settlement cloud map after excavation at different steps: (<b>a</b>) first layer excavation; (<b>b</b>) second layer excavation; (<b>c</b>) third layer excavation.</p>
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<p>(<b>a</b>) Numerical simulation of soil settlement curve around foundation pit; (<b>b</b>) schematic diagram of the distance from the edge of the pit.</p>
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<p>Displacement cloud diagram of each supporting structure: (<b>a</b>) Underground diaphragm wall; (<b>b</b>) Back wall of box-type retaining wall; (<b>c</b>) Three-axis mixing pile; (<b>d</b>) Bored pile.</p>
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<p>Influence of box-type retaining wall dimensions: (<b>a</b>,<b>b</b>) Horizontal displacements of front wall of box-type retaining wall; (<b>c</b>,<b>d</b>) Surrounding surface settlements with variations in the box length and width.</p>
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<p>Influence of box-type retaining wall dimensions: (<b>a</b>,<b>b</b>) Horizontal displacements of front wall of box-type retaining wall; (<b>c</b>,<b>d</b>) Surrounding surface settlements with variations in the box length and width.</p>
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<p>Influence of the internal fill material of box-type retaining walls: (<b>a</b>) Horizontal displacement of the front wall of box-type retaining wall; (<b>b</b>) settlement of the surrounding surface from the edge of foundation pit.</p>
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<p>Influence of the internal fill material of box-type retaining walls: (<b>a</b>) Horizontal displacement of the front wall of box-type retaining wall; (<b>b</b>) settlement of the surrounding surface from the edge of foundation pit.</p>
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<p>Influence of concrete materials used for pouring box-type retaining walls: (<b>a</b>) Horizontal displacement of the front wall of box-type retaining wall; (<b>b</b>) settlement of the surrounding surface from the edge of foundation pit.</p>
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<p>Influence of concrete materials used for pouring box-type retaining walls: (<b>a</b>) Horizontal displacement of the front wall of box-type retaining wall; (<b>b</b>) settlement of the surrounding surface from the edge of foundation pit.</p>
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17 pages, 27885 KiB  
Article
Interaction Between Concrete and FRP Laminate in Structural Members Composed of Reused Wind Turbine Blades Filled with Concrete
by Anna Halicka, Lidia Buda-Ożóg, Mirosław Broniewicz, Łukasz Jabłoński, Joanna Zięba and Filip Broniewicz
Materials 2024, 17(24), 6186; https://doi.org/10.3390/ma17246186 - 18 Dec 2024
Viewed by 497
Abstract
The lifecycle of wind turbine blades is around 20–25 years. This makes studies on the reuse of dismantled blades an urgent need for our generation; however, their recycling is very difficult due to the specific makeup of their composite material. In this study, [...] Read more.
The lifecycle of wind turbine blades is around 20–25 years. This makes studies on the reuse of dismantled blades an urgent need for our generation; however, their recycling is very difficult due to the specific makeup of their composite material. In this study, the authors determined a concept for the reuse of turbine blade sections filled with concrete for geotechnical structures, retaining the walls, piles, or parts of their foundations. Working out detailed structural solutions to the above problem should be preceded by the identification of material parameters. In particular, getting to know the interface stress-strain characteristics is crucial. Therefore, this research focuses on the cooperation between recycled FRP composites and concrete in load-carrying, including experiments and numerical analyses. Regarding the two types of destructive stress, which may occur at the interface under both compression and bending, two types of tests were executed: the ‘push-out test’, modelling the interface’s answer to shear stress, and the ‘pull-off test’, demonstrating the interface’s reaction to normal stress. Additionally, the strength parameters of the materials used were tested. The numerical model for the push-out process was calibrated on the basis of the tests, and this way the shear bond strength and the coefficient of friction between the concrete and the recycled FRP laminate were assessed. Full article
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<p>Concepts of reuse of turbine blade sections filled with concrete: (<b>A</b>) pile foundations under base beams or plates (piles made of Vestas-type spar caps filled with concrete), (<b>B</b>) cantilevered retaining walls, excavation linings, or shoring walls (cantilever beams made of Vestas-type spar caps filled with concrete and plating made of fragments of LM blades), (<b>C</b>) cross-section of hybrid element.</p>
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<p>Schemas of bond tests and specimens prepared for testing: 1—concrete, 2—composite part of turbine blade, 3—closing board enabling the concreting, 4—applied load.</p>
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<p>Dismantled turbine blade parts used in the experiment: (<b>A</b>) turbine blades in the scrap heap; (<b>B</b>) section cutout of turbine blade for testing: 1—spar cap, 2—adhesive, 3—aerodynamical shell (outer balsa, polyurethane foam filling, and inner FRP composite), 4—composite strengthening of leading edge, 5—run-off edge.</p>
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<p>Specimens prepared for push-out testing: (<b>A</b>) FRP laminate tube with marked main internal dimensions for push-off testing, (<b>B</b>,<b>C</b>) tubes filled with concrete.</p>
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<p>Specimen cut from spar cap: (<b>A</b>) cross-section, where internal layers are visible; (<b>B</b>) surface of FRP laminate.</p>
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<p>Execution of push-off test.</p>
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<p>Pull-off test: (<b>A</b>) execution; (<b>B</b>) the specimen after testing.</p>
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<p>Discrete 3D model and applied boundary conditions of push-out test.</p>
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<p>‘Traction–separation’ response assumed in FEM.</p>
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<p>Experimental ‘load–displacement of concrete in relation to FRP tube’ relations; the dashed line represents the numerical analysis results (see <a href="#sec3dot3-materials-17-06186" class="html-sec">Section 3.3</a>, below).</p>
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<p>Post-testing push-out specimens.</p>
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<p>Response of the FEM specimen during the course of the push-off test.</p>
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<p>Stress distributions in the FEM specimen interface between the concrete and FRP tube, in MPa.</p>
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33 pages, 60830 KiB  
Article
Assessment of the Accuracy of Terrestrial Laser Scanners in Detecting Local Surface Anomaly
by Ali Algadhi, Panos Psimoulis, Athina Grizi and Luis Neves
Remote Sens. 2024, 16(24), 4647; https://doi.org/10.3390/rs16244647 - 11 Dec 2024
Viewed by 668
Abstract
The surface anomaly is a common defect for structures that resist lateral stresses, such as retaining walls. The accurate detection of an anomaly using contactless techniques, such as the Terrestrial Laser Scanner (TLS), is significant for the reliable structural assessment. The influence of [...] Read more.
The surface anomaly is a common defect for structures that resist lateral stresses, such as retaining walls. The accurate detection of an anomaly using contactless techniques, such as the Terrestrial Laser Scanner (TLS), is significant for the reliable structural assessment. The influence of the scanning geometry on the accuracy of the TLS point-clouds was investigated in previous studies; however, a deeper analysis is needed to investigate their impact in the context of structural health monitoring. This paper aims to empirically assess the performance of the TLS in detecting surface anomalies, with respect to the scanning distance and angle of incidence in two cases: (i) when both the reference and deformed clouds are taken from the same scanning position, and (ii) the scans are from different positions. Furthermore, the paper examines the accuracy of estimating the depth of the anomaly using three cloud comparison techniques (i.e., C2C, C2M, and M3C2 methods). The results show that the TLS is capable of detecting the surface anomaly for distances between 2 and 30 m and angles of incidence between 90° and 30°, with a tolerance of within a few millimeters. This is achieved even for the case where scans from different locations (i.e., angles and distances) are applied. Full article
(This article belongs to the Section Environmental Remote Sensing)
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<p>Design of the experimental device (i.e., papier-mâché sheet): (<b>a</b>) front view showing the papier-mâché sheet and the anomaly, (<b>b</b>) side view showing the introduced tilt, (<b>c</b>) rear view for the scenario with no tilt; showing the bolt that was used for attaching the anomaly.</p>
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<p>Scan setup for the papier-mâché experiment: (<b>a</b>) overview of the experiment setup, and (<b>b</b>) plan view of the experiment site showing (i) the thirteen scanning positions, (ii) the papier-mâché sheet, (iii) the three sphere targets, and (iv) the final coordinate system (<span class="html-italic">x</span>- and <span class="html-italic">y</span>-axes) that were aligned to the transverse and lateral axes of the papier-mâché sheet.</p>
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<p>Flowchart of the measurements, processing, and analysis methods.</p>
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<p>Front and side views showing a sample of the point-clouds at the scanning position of 10 m for the papier-mâché sheet at the three deformation scenarios; (i) the initial undeformed state, (ii) the state where no tilt was introduced but only an anomaly at the center of the sheet, and (iii) the state where both the anomaly and global tilt were introduced to the scanned surface.</p>
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<p>The M3C2 distance for the scanning position of 10 m, compared to the initial undeformed state.</p>
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<p>The change in intensity for the scanning position of 10 m, compared to the initial undeformed state.</p>
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<p>The change in the direction of the normal vector along the <span class="html-italic">x</span>-, <span class="html-italic">y</span>-, and <span class="html-italic">z</span>-axes for the scanning position of 10 m, compared to the initial undeformed state.</p>
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<p>The accuracy of estimating the maximum depth of the anomaly using three cloud-comparison techniques: (<b>a</b>) C2C distance, (<b>b</b>) C2M distance, and (<b>c</b>) M3C2 distance.</p>
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<p>Overview of the monitored case study, showing the area of interest.</p>
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<p>The change in the intensity values for the scans that were taken in November 2020, compared to the reference clouds (i.e., orthogonal and oblique).</p>
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<p>The change in the intensity values for each orthogonal scan that was acquired in the morning of each scanning day, compared to the reference cloud that was taken on 4 November 2020.</p>
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<p>The change in direction of the normal vector along the <span class="html-italic">x</span>-axis for each orthogonal scan that was acquired in the morning of each scanning day, compared to the reference cloud that was taken on the 4 November 2020.</p>
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<p>The change in direction of the normal vector along the <span class="html-italic">y</span>-axis for each orthogonal scan that was acquired on the morning of each scanning day, compared to the reference cloud that was taken on 4 November 2020.</p>
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<p>The change in direction of the normal vector along the <span class="html-italic">z</span>-axis for each orthogonal scan that was acquired on the morning of each scanning day, compared to the reference cloud that was taken on 4 November 2020.</p>
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<p>M3C2 distance for the papier-mâché sheet, compared to the initial scan (at the same scanning position) with no anomaly: (<b>a</b>) with no tilt, and (<b>b</b>) with tilt.</p>
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<p>M3C2 distance for the papier-mâché sheet, compared to the initial scan (at 10 m) with no anomaly: (<b>a</b>) with no tilt, and (<b>b</b>) with tilt.</p>
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<p>Change in intensity of the papier-mâché sheet, compared to the initial scan (at the same scanning position) with no anomaly: (<b>a</b>) with no tilt, and (<b>b</b>) with tilt.</p>
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<p>Change in intensity of the papier-mâché sheet, compared to the initial scan (at 10 m) with no anomaly: (<b>a</b>) with no tilt, and (<b>b</b>) with tilt.</p>
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<p>The intensity of the reflected signal on each orthogonal scan that was acquired on the morning of each scanning day.</p>
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<p>Change in the normal vector along the <span class="html-italic">x</span>-axis, compared to the initial scan (at the same scanning position) with no anomaly: (<b>a</b>) with no tilt, and (<b>b</b>) with tilt.</p>
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<p>Change in the normal vector along the <span class="html-italic">x</span>-axis, compared to the initial scan (at 10 m) with no anomaly: (<b>a</b>) with no tilt, and (<b>b</b>) with tilt.</p>
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<p>Change in the normal vector along the <span class="html-italic">y</span>-axis, compared to the initial scan (at the same scanning position) with no anomaly: (<b>a</b>) with no tilt, and (<b>b</b>) with tilt.</p>
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<p>Change in the normal vector along the <span class="html-italic">y</span>-axis, compared to the initial scan (at 10 m) with no anomaly: (<b>a</b>) with no tilt, and (<b>b</b>) with tilt.</p>
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<p>Change in the normal vector along the <span class="html-italic">z</span>-axis, compared to the initial scan (at the same scanning position) with no anomaly: (<b>a</b>) with no tilt, and (<b>b</b>) with tilt.</p>
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<p>Change in the normal vector along the <span class="html-italic">z</span>-axis, compared to the initial scan (at 10 m) with no anomaly: (<b>a</b>) with no tilt, and (<b>b</b>) with tilt.</p>
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<p>The change in the direction of the normal vector along the <span class="html-italic">x</span>-axis for the scans that were taken in November 2020, compared to the reference clouds (i.e., orthogonal and oblique).</p>
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<p>The change in the direction of the normal vector along the <span class="html-italic">y</span>-axis for the scans that were taken in November 2020, compared to the reference clouds (i.e., orthogonal and oblique).</p>
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<p>The change in the direction of the normal vector along the <span class="html-italic">z</span>-axis for the scans that were taken in November 2020, compared to the reference clouds (i.e., orthogonal and oblique).</p>
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29 pages, 11116 KiB  
Article
Displacement Estimation Performance of a Cost-Effective 2D-LiDAR-Based Retaining Wall Displacement Monitoring System
by Jun-Sang Kim and Young Suk Kim
Remote Sens. 2024, 16(24), 4644; https://doi.org/10.3390/rs16244644 - 11 Dec 2024
Viewed by 579
Abstract
Monitoring the displacement of retaining walls is essential for maintaining their stability. Traditional displacement monitoring by inclinometer is costly and time-consuming, owing to the need for manual measurements. A recently developed 2D-LiDAR-based retaining wall displacement monitoring system offers advantages over traditional methods, such [...] Read more.
Monitoring the displacement of retaining walls is essential for maintaining their stability. Traditional displacement monitoring by inclinometer is costly and time-consuming, owing to the need for manual measurements. A recently developed 2D-LiDAR-based retaining wall displacement monitoring system offers advantages over traditional methods, such as easy installation and dismantling, as well as the cost-effective monitoring of three-dimensional displacement compared to terrestrial laser scanners (TLSs). However, a previous study did not account for the actual deformation of the retaining wall, potentially compromising the reliability of the displacement estimation. This study aims to assess the displacement estimation performance of the system by using a retaining wall that simulates real-world deformations, considering key parameters related to the displacement estimation algorithm and the quality of point cloud data. Using the multiple model-to-model cloud comparison algorithm and a developed algorithm for filtering duplicate point cloud data, the system’s average performance across various deformation types yielded mean absolute error (MAE), MAEDmax, and compound error values of 1.7, 2.2, and 2.0 mm, respectively. The results demonstrate that even a 2D-LiDAR, which has lower precision than a TLS, can effectively monitor retaining wall displacement through the post-processing of point cloud data. Full article
(This article belongs to the Special Issue 3D Scene Reconstruction, Modeling and Analysis Using Remote Sensing)
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<p>Configuration diagram of the 2D-LiDAR-based retaining wall displacement monitoring system.</p>
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<p>Measurement process of 2D-LiDAR-based retaining wall displacement monitoring system.</p>
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<p>Estimation of displacement using algorithms from the C2C series and the C2M algorithm.</p>
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<p>Estimation of displacement using the M3C2 algorithm. (<b>a</b>) Generating normal vectors for each core point. (<b>b</b>) Estimation of the displacement of the baseline and comparison point data using cylinders.</p>
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<p>Overall evaluation methodology for the displacement estimation performance of the 2D-LiDAR-based retaining wall displacement monitoring system.</p>
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<p>Example of duplicate point cloud data, according to the number of rotations.</p>
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<p>Method and problems of filtering duplicate point cloud data based on voxel grid downsampling. (<b>a</b>) Results of voxel grid downsampling. (<b>b</b>) Problems of voxel grid downsampling caused by voxel positioning. (<b>c</b>) Problems of voxel grid downsampling caused by moving objects.</p>
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<p>Method and problems of filtering duplicate point cloud data based on voxel grid downsampling. (<b>a</b>) Results of voxel grid downsampling. (<b>b</b>) Problems of voxel grid downsampling caused by voxel positioning. (<b>c</b>) Problems of voxel grid downsampling caused by moving objects.</p>
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<p>Algorithm for filtering duplicate point cloud data based on a spherical coordinate system.</p>
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<p>Definitions of retaining wall deformation types and inclinometer data.</p>
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<p>Results of the construction of the baseline and deformation simulated retaining walls.</p>
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<p>Process for evaluating the displacement estimation performance using TLS and simulated retaining walls.</p>
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<p>Results of the point cloud data collection by the 2D-LiDAR-based retaining wall displacement monitoring system and the TLS. (<b>a</b>) Results of point cloud data collection and ROIs for displacement estimation by the 2D-LiDAR-based retaining wall displacement monitoring system. (<b>b</b>) Results of point cloud data collection and ROIs for displacement estimation by TLS.</p>
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<p>Results of the point cloud data collection by the 2D-LiDAR-based retaining wall displacement monitoring system and the TLS. (<b>a</b>) Results of point cloud data collection and ROIs for displacement estimation by the 2D-LiDAR-based retaining wall displacement monitoring system. (<b>b</b>) Results of point cloud data collection and ROIs for displacement estimation by TLS.</p>
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<p>Displacement estimation performance results by displacement estimation algorithm for different types of deformation.</p>
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<p>Histogram results for the displacement estimation algorithms and deformation types. (<b>a</b>) Simulated retaining wall with upper deformation. (<b>b</b>) Simulated retaining wall with middle deformation. (<b>c</b>) Simulated retaining wall with lower deformation. (<b>d</b>) Simulated retaining wall with compound deformation.</p>
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<p>Analytical results of the displacement performance for different types of deformation, according to changes in the normal vector diameter.</p>
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<p>Analytical results of the displacement performance, according to variations in the cylinder diameter and deformation type.</p>
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<p>Analytical results of the differences between the ground truth and system displacement results for different types of deformation, according to changes in the cylinder diameter. (<b>a</b>) Simulated retaining wall with upper deformation. (<b>b</b>) Simulated retaining wall with middle deformation. (<b>c</b>) Simulated retaining wall with lower deformation. (<b>d</b>) Simulated retaining wall with compound deformation.</p>
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<p>Analytical results of the differences between the ground truth and system displacement results for different types of deformation, according to changes in the cylinder diameter. (<b>a</b>) Simulated retaining wall with upper deformation. (<b>b</b>) Simulated retaining wall with middle deformation. (<b>c</b>) Simulated retaining wall with lower deformation. (<b>d</b>) Simulated retaining wall with compound deformation.</p>
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<p>Analytical results of the displacement performance by different types of deformation, based on the rotation count and algorithm for filtering duplicate point cloud data. (<b>a</b>) Simulated retaining wall with upper deformation. (<b>b</b>) Simulated retaining wall with middle deformation. (<b>c</b>) Simulated retaining wall with lower deformation. (<b>d</b>) Simulated retaining wall with compound deformation.</p>
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<p>Histogram and scatter plot results of each deformation type for the first rotation and five rotations.</p>
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<p>Histogram and scatter plot results of each deformation type for the first rotation and five rotations.</p>
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<p>Comparison of the displacement results of 2D-LiDAR-based retaining wall displacement monitoring system and estimated displacement results of the TLS (ground truth).</p>
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33 pages, 59097 KiB  
Article
Street Canyon Vegetation—Impact on the Dispersion of Air Pollutant Emissions from Road Traffic
by Paulina Bździuch, Marek Bogacki and Robert Oleniacz
Sustainability 2024, 16(23), 10700; https://doi.org/10.3390/su162310700 - 6 Dec 2024
Viewed by 679
Abstract
Roadside vegetation helps to retain air pollutants emitted by road traffic. On the other hand, its presence makes it difficult to ventilate street canyons. The paper examines the influence of vegetation on the dispersion of air pollution generated by road traffic, using the [...] Read more.
Roadside vegetation helps to retain air pollutants emitted by road traffic. On the other hand, its presence makes it difficult to ventilate street canyons. The paper examines the influence of vegetation on the dispersion of air pollution generated by road traffic, using the example of two street canyons—both-sided and one-sided street canyons. The study was conducted taking into account the actual emission conditions occurring on the analyzed road sections estimated using the HBEFA methodology. Subsequently, a three-dimensional pollution dispersion model named MISKAM was employed to simulate the air pollutant dispersion conditions in the analyzed street canyons. The modelling results were compared with the measurement data from air quality monitoring stations located in these canyons. The obtained results indicated that the presence of vegetation can significantly impact on the air dispersion of traffic-related exhaust and non-exhaust emissions. The impact of vegetation is more pronounced in the case of a street canyon with dense, high-rise development on both sides than in the case of a street canyon with such development on only one side. The results for the both-sided street canyon demonstrate that the discrepancy between the scenario devoid of vegetation and the scenario with vegetation was approximately 5 µg/m3 (10%) for PM10 and approximately 54 µg/m3 (45%) for NOx, with the former scenario showing lower values than the latter. Nevertheless, the scenario with the vegetation exhibited a lesser discrepancy with the air quality measurements. Vegetation functions as a natural barrier, reducing wind speed in the street canyon, which in turn limits the spread of pollutants in the air, leading to pollutant accumulation near the building walls that form the canyon. Consequently, atmospheric dispersion modelling must consider the presence of vegetation to accurately evaluate the effects of road traffic emissions on air quality in urban areas, particularly in street canyons. The results of this study may hold importance for urban planning and decision-making regarding environmental management in cities aimed at improving air quality and public health. Full article
(This article belongs to the Special Issue Air Quality Characterisation and Modelling—2nd Edition)
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<p>Research area—the city of Krakow (Poland) with the location of air quality monitoring and meteorological stations, and computational areas marked.</p>
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<p>Detailed locations of street canyons and computational areas for modelling the dispersion of traffic-related pollutants.</p>
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<p>Visualization of shapefile input data for the calculation area at the traffic air quality monitoring station (AQMS) at the Krasińskiego Avenue street canyon.</p>
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<p>Visualization of shapefile input data for the calculation area at the traffic air quality monitoring station (AQMS) at the Dietla street canyon.</p>
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<p>Mapping in WinMISKAM of the computational area in the Krasińskiego Avenue street canyon: (<b>a</b>) visualization of meshing for the study area; (<b>b</b>) fragment of the mesh for the AQMS location area (red star—MpKrakAlKras). Legend: black objects—vegetation outline; pink objects—roads, emission source; other coloured objects—visualization of buildings.</p>
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<p>Mapping in WinMISKAM of the computational area in the Dietla street canyon: (<b>a</b>) visualization of meshing for the study area; (<b>b</b>) fragment of the mesh for the AQMS location area (red star—MpKrakDietla). Legend: black objects—vegetation outline; pink objects—roads, emission source; other coloured objects—visualization of buildings.</p>
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<p>Simulation results of PM<sub>10</sub> dispersion in the street canyon of Krasińskiego Avenue for variant K1. Top left: cross-sectional projection of the PM<sub>10</sub> dispersion simulation at the AQMS site.</p>
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<p>Simulation results of PM<sub>10</sub> dispersion in the street canyon of Krasińskiego Avenue for variant K2. Top left: cross-sectional projection of the PM<sub>10</sub> dispersion simulation at the AQMS site.</p>
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<p>Simulation results of NOx dispersion in the street canyon of Krasińskiego Avenue for variant K1. Top left: cross-sectional projection of the NOx dispersion simulation at the AQMS site.</p>
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<p>Simulation results of NOx dispersion in the street canyon of Krasińskiego Avenue for variant K2. Top left: cross-sectional projection of the NOx dispersion simulation at the AQMS site.</p>
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<p>Simulation results of PM<sub>10</sub> dispersion in the Dietla street canyon, with AQMS marked for variant D1. Bottom right: cross-sectional projection of the PM<sub>10</sub> dispersion simulation at the AQMS site.</p>
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<p>Simulation results of PM<sub>10</sub> dispersion in the Dietla street canyon for variant D2. Bottom right: cross-sectional projection of the PM<sub>10</sub> dispersion simulation at the AQMS site.</p>
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<p>Simulation results of NOx dispersion in the Dietla street canyon for variant D1. Bottom right: cross-sectional projection of the NOx dispersion simulation at the AQMS site.</p>
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<p>Simulation results of NOx dispersion in the Dietla street canyon for variant D2. Bottom right: cross-sectional projection of the NOx dispersion simulation at the AQMS site.</p>
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<p>The optimum mesh distribution in the street canyon for the post-horizontal projection in the MISKAM model. The yellow element indicates the horizontal position of the grid point most often considered when analyzing the results of pollutant dispersion modeling in street canyons.</p>
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<p>The optimum mesh distribution in the street canyon for the vertical projection in the MISKAM model. The red element indicates the vertical position of the grid point most often considered when analyzing the results of pollutant dispersion modeling in street canyons.</p>
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<p>Krasińskiego Avenue street canyon study area (own study based on Google Earth map).</p>
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<p>Dietla street canyon study area (own study based on Google Earth map).</p>
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<p>Comparison of simulation results of PM<sub>10</sub> dispersion in the Krasińskiego Avenue street canyon for the horizontal section: (<b>a</b>) variant K1 (without vegetation); (<b>b</b>) variant K2 (with vegetation). Modelling results at height: 1.2–1.8 m.</p>
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<p>Comparison of simulation results of NOx dispersion in the Krasińskiego Avenue street canyon for the horizontal section: (<b>a</b>) variant K1 (without vegetation); (<b>b</b>) variant K2 (with vegetation). Modelling results at height: 1.2–1.8 m.</p>
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<p>Comparison of simulation results of PM<sub>10</sub> dispersion in the Dietla street canyon for the horizontal section: (<b>a</b>) variant D1 (without vegetation); (<b>b</b>) variant D2 (with vegetation). Modelling results at height: 1.2–1.8 m.</p>
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<p>Comparison of simulation results of NOx dispersion in the Dietla street canyon for the horizontal section: (<b>a</b>) variant D1 (without vegetation); (<b>b</b>) variant D2 (with vegetation). Modelling results at height: 1.2–1.8 m.</p>
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<p>Comparison of simulation results of PM<sub>10</sub> dispersion in the Krasińskiego Avenue street canyon for a vertical section: (<b>a</b>) variant K1 (without vegetation); (<b>b</b>) variant K2 (with vegetation). Modelling results for the cross-section passing through the MpKrakAlKras AQMS site.</p>
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<p>Comparison of simulation results of NOx dispersion in the Krasińskiego Avenue street canyon for a vertical section: (<b>a</b>) variant K1 (without vegetation); (<b>b</b>) variant K2 (with vegetation). Modelling results for the cross-section passing through the MpKrakAlKras AQMS site.</p>
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<p>Comparison of simulation results of NOx dispersion in the Krasińskiego Avenue street canyon for a vertical section: (<b>a</b>) variant K1 (without vegetation); (<b>b</b>) variant K2 (with vegetation). Modelling results for the cross-section passing through the MpKrakAlKras AQMS site.</p>
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<p>Comparison of simulation results of PM<sub>10</sub> dispersion in the Dietla street canyon for a vertical section: (<b>a</b>) variant D1 (without vegetation); (<b>b</b>) variant D2 (with vegetation). Modelling results for the cross-section passing through the MpKrakDietla AQMS site.</p>
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<p>Comparison of simulation results of NOx dispersion in the Dietla street canyon for a vertical section: (<b>a</b>) variant D1 (without vegetation); (<b>b</b>) variant D2 (with vegetation). Modelling results for the cross-section passing through the MpKrakDietla AQMS site.</p>
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<p>Comparison of modelling results of average annual air pollutant concentrations in the cross-section of the Krasińskiego Avenue street canyon passing through the MpKrakAlKras AQMS site for variants K1 (without vegetation) and K2 (with vegetation): (<b>a</b>) PM<sub>10</sub>; (<b>b</b>) NOx. Data for simulation at height: 1.2–1.8 m.</p>
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<p>Comparison of modelling results of average annual air pollutant concentrations in the cross-section of the Krasińskiego Avenue street canyon passing through the MpKrakAlKras AQMS site for variants K1 (without vegetation) and K2 (with vegetation): (<b>a</b>) PM<sub>10</sub>; (<b>b</b>) NOx. Data for simulation at height: 1.2–1.8 m.</p>
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<p>Comparison of modelling results of average annual air pollutant concentrations in the cross-section of the Dietla street canyon passing through the MpKrakDietla AQMS site for variants D1 (without vegetation) and D2 (with vegetation): (<b>a</b>) PM<sub>10</sub>; (<b>b</b>) NOx. Data for simulation at height: 1.2–1.8 m.</p>
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<p>Box-plot graph illustrating the variability of percentage differences between the average annual concentrations of the analyzed pollutants in individual variants. Simulation results at a height of 1.2–1.8 m for the cross-section of a given street canyon passing through the AQMS with a step of 2 m: (<b>a</b>) Krasińskiego Avenue street canyon; (<b>b</b>) Dietla street canyon.</p>
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<p>Comparison of simulation results of NOx dispersion in the Krasińskiego Avenue street canyon for the axial longitudinal vertical section: (<b>a</b>) variant K1 (without vegetation); (<b>b</b>) variant K2 (with vegetation).</p>
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<p>Comparison of simulation results of NOx dispersion in the Krasińskiego Avenue street canyon for the axial longitudinal vertical section: (<b>a</b>) variant K1 (without vegetation); (<b>b</b>) variant K2 (with vegetation).</p>
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19 pages, 5731 KiB  
Article
New-Generation Antibacterial Agent—Cellulose-Binding Thermostable TP84_Endolysin
by Małgorzata Ponikowska, Joanna Żebrowska and Piotr M. Skowron
Int. J. Mol. Sci. 2024, 25(23), 13111; https://doi.org/10.3390/ijms252313111 - 6 Dec 2024
Viewed by 945
Abstract
The increasing antibiotic resistance among bacteria challenges the biotech industry to search for new antibacterial molecules. Endolysin TP84_28 is a thermostable, lytic enzyme, encoded by the bacteriophage (phage) TP-84, and it effectively digests host bacteria cell wall. Biofilms, together with antibiotic resistance, are [...] Read more.
The increasing antibiotic resistance among bacteria challenges the biotech industry to search for new antibacterial molecules. Endolysin TP84_28 is a thermostable, lytic enzyme, encoded by the bacteriophage (phage) TP-84, and it effectively digests host bacteria cell wall. Biofilms, together with antibiotic resistance, are major problems in clinical medicine and industry. The challenge is to keep antibacterial molecules at the site of desired action, as their diffusion leads to a loss of efficacy. The TP84_28 endolysin gene was cloned into an expression-fusion vector, forming a fusion gene cbd_tp84_28_his with a cellulose-binding domain from the cellulase enzyme. The Cellulose-Binding Thermostable TP84_Endolysin (CBD_TP84_28_His) fusion protein was biosynthesized in Escherichia coli and purified. Thermostability and enzymatic activities against various bacterial species were measured by a turbidity reduction assay, a spot assay, and biofilm removal. Cellulose-binding properties were confirmed via interactions with microcellulose and cellulose paper-based immunoblotting. The high affinity of the CBD allows for a high concentration of the fusion enzyme at desired target sites such as cellulose-based wound dressings, artificial heart valves and food packaging. CBD_TP84_28_His exhibits a lytic effect against thermophilic bacteria Geobacillus stearothemophilus, Thermus aquaticus, Bacillus stearothermophilus, and Geobacillus ICI and minor effects against mesophilic Bacillus cereus and Bacillus subtilis. CBD_TP84_28_His retains full activity after preincubation in the temperatures of 30–65 °C and exhibits significant activity up to its melting point at 73 °C. CBD_TP84_28_His effectively reduces biofilms. These findings suggest that integrating CBDs into thermostable endolysins could enable the development of targeted antibacterial recombinant proteins with diverse clinical and industrial applications. Full article
(This article belongs to the Section Molecular Microbiology)
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<p>SDS-PAGE analysis of the purified CBD_TP84_28_His. M, PageRuler Plus Stained Protein Ladder; E, CBD_TP84_28_His. Arrow points at a band corresponding in size to the endolysin CBD_TP84_28_His (64.2 kDa).</p>
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<p>Recombinant fusion endolysin CBD_TP84_28_His activity evaluation—spot assay. Top row (<b>a</b>) shows activity of CBD_TP84_28_His against mesophilic bacteria: <span class="html-italic">B. cereus</span>, <span class="html-italic">B. subtilis</span>, <span class="html-italic">E. coli.</span> Bottom row (<b>b</b>) shows activity of CBD_TP_84_28_His against thermophilic bacteria: <span class="html-italic">G. stearothermophilus</span> strain 10, <span class="html-italic">T. aquaticus</span>, <span class="html-italic">Geobacillus</span> ICI, <span class="html-italic">B. stearothermophilus.</span> Arrows indicate where CBD_TP84_28_His solution was spotted and transparent circles on the bacterial lawn indicate lytic effect of the enzyme.</p>
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<p>CBD_TP84_28_His activity evaluation—TRA on various bacterial strains. The graph shows the reduction in relative OD<sub>600</sub> (ratio of OD<sub>600</sub> of the sample treated with CBD_TP84_28_His to the OD<sub>600</sub> of the control) in bacterial substrate suspensions upon addition of purified CBD_TP84_28_His to the final concentration of 1.43 µg/mL at 55 °C. Top row (<b>a</b>) shows activity of CBD_TP84_28_His against mesophilic bacteria: <span class="html-italic">B. cereus</span>, <span class="html-italic">B. subtilis</span>, <span class="html-italic">E. coli</span>. Bottom rows (<b>b</b>) show activity of CBD_TP_84_28_His against thermophilic bacteria: <span class="html-italic">G. stearothermophilus</span> strain 10, <span class="html-italic">T. aquaticus</span>, <span class="html-italic">Geobacillus</span> ICI, <span class="html-italic">B. stearothermophilus</span>. Results are presented as means ± SD of three independent experiments. Statistically significant differences were determined by one-way ANOVA with Dunnett’s test and marked as (***)—<span class="html-italic">p</span>-value  &lt; 0.001, (****)—<span class="html-italic">p</span>-value  &lt; 0.0001.</p>
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<p>Comparison of activity of the CBD_TP84_28_His and recombinant TP84_28_His. The graph shows reduction in OD<sub>600</sub> in <span class="html-italic">G. stearothermophilus</span> strain 10, resuspended in buffer R, after addition of equimolar amounts of CBD_TP84_28_His, TP84_28_His or the reaction buffer alone [control (-)]. Results are presented as means ± SD of three independent experiments. Statistical significance was assessed using two-way ANOVA with Tukey’s post hoc test, with symbols indicating significant differences (<span class="html-italic">p</span>-value &lt; 0.05) between groups: (<span>$</span>)—CBD_TP84_28_His vs. TP84_28_His, (^)—CBD_TP84_28_His vs. control (-), (#)—TP84_28_His vs. control (-).</p>
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<p>Inhibition of biofilm formation by <span class="html-italic">G. stearothermophilus</span> strain 10. The graph (<b>a</b>) shows OD<sub>600</sub> measurements of <span class="html-italic">G. stearothermophilus</span> strain 10, cultivated on microtiter plate with addition of 0.05–50 µg of CBD_TP84_28_His for 24 h at 55 °C. The picture below the chart (<b>b</b>) shows biofilm stained with crystal violet: control on the left and samples treated with increasing amounts of the recombinant fusion endolysin CBD_TP84_28_His. Results are presented as means ± SD of three independent experiments. Statistically significant differences were determined by one-way ANOVA with Dunnett’s test and marked as (****)—<span class="html-italic">p</span>-value &lt; 0.0001.</p>
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<p>CBD_TP84_28_His thermal stability evaluation. The lytic activity of CBD_TP84_28_His was assayed in optimal conditions after preincubation at various temperatures in a gradient thermocycler. The graph shows a reduction in OD<sub>600</sub> in <span class="html-italic">G. stearothermophilus</span> strain 10 suspension in buffer R after the addition of CBD_TP84_28_His, preincubated for 30 min at temperatures of 37.7–95.8 °C and autoclaving conditions (121 °C, 20 min). As a negative control, buffer R was added instead of CBD_TP84_28_His. Measurements were taken using the Tecan microplate reader. This graph shows the final single-repeat experiment, including all the temperature points. Prior to the combined experiment, each temperature was evaluated with 3 repetitions separately. This arrangement was necessary to eliminate uncontrolled reaction progression due to the high number of samples (14) needing to be processed simultaneously.</p>
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<p>Interactions comparison of recombinant enzymes: endolysin TP84_28_His and CBD_TP84_28_His with µC. (<b>a</b>) The binding of TP84_28_His and CBD_TP84_28_His to µC in a 96-well plate using the detection of His-tag for Western blots (Method 2.2.3.1). Both proteins have a His-tag that is located at the C-terminus, which allows for the detection of the protein attached to the µC using anti-His antibodies. (<b>b</b>) A dot blot assay of endolysin activity using the host <span class="html-italic">G. stearothermophilus</span> strain 10. A control—µC and 1 × PBS dot—was applied, and µC complexes formed with each of the enzymes TP84_28_His and CBD_TP84_28_His, which were applied to the agar plate with spread <span class="html-italic">G. stearothermophilus</span> strain 10. (<b>c</b>) An SDS-PAGE comparative analysis of the formation of insoluble µC complexes with CBD_TP84_28 and with TP84_28_His. Lane M, PageRuler Plus Stained Protein Ladder; lane K, untreated TP84_28_His (<b>left</b>)/CBD_TP84_28 (<b>right</b>); lane 1, insoluble µC complex formed with TP84_28_His (<b>left</b>)/CBD_TP84_28_His (<b>right</b>); lane 2, supernatants of complex formation—unbound protein; lane 3, washing the supernatant of the complexes with 1 × PBS. (<b>d</b>) µC complex formed with each of the enzymes, TP84_28_His and CBD_TP84_28_His, which were applied to the agar plate with spread <span class="html-italic">G. stearothermophilus</span> strain 10 to measure µC complex activity; dot K, TP84_28_His/CBD_TP84_28_His; dot 1, formed and washed µC complexes with TP84_28_His/CBD_TP84_28_His; dot 2, supernatants of insoluble µC complex formation; dot 3, washes of the complexes with 1 × PBS; dots 4, first washes of the complex with water; K1, control of buffer R; K2, control of µC; K3, control of PBS; K4, control of buffer A.</p>
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<p>CBD_TP84_28_His interaction with cellulose paper: (<b>a</b>) scheme showing CBD binding to cellulose filter paper and His-Tag detection with anti-His-HRP antibody, which enables visualization of the reaction after colour development using 3,3′-diaminobenzidine (DAB). (<b>b</b>) Cellulose filter paper with spots: PBS buffer (control), TP84_28_His and CBD_TP84_28_His.</p>
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20 pages, 7041 KiB  
Article
Study on Calibration Tests for Interface-Type Earth Pressure Cell Based on Matching Error Analysis
by Mingyu Li, Longwei Zhu, Jicheng Shu, Zhenzhen Lu and Yunlong Liu
Sensors 2024, 24(23), 7778; https://doi.org/10.3390/s24237778 - 5 Dec 2024
Viewed by 633
Abstract
The stress status of a soil pressure cell placed in soil is very different from its stress state in a uniform fluid medium. The use of the calibration coefficient provided by the soil pressure cell manufacturer will produce a large error. In order [...] Read more.
The stress status of a soil pressure cell placed in soil is very different from its stress state in a uniform fluid medium. The use of the calibration coefficient provided by the soil pressure cell manufacturer will produce a large error. In order to improve the measurement accuracy of the interface-type earth pressure cell placed in soil, this paper focuses on a single-membrane resistive earth pressure cell installed on the surface of a structure, analyzing the influence of loading and unloading cycles, the thickness and particle size of the sand filling, and the depth of the earth pressure cell inserted in the structure on the calibration curve and matching error, which were analyzed through calibration tests. The results show that when the sand filling thickness is less than D (D is the diameter of the earth pressure cell), the calibration curve is unstable in relation to the increase in the number of loading and unloading cycles, which will cause the sand calibration coefficient used for stress conversion to not be used normally. When the sand filling thickness in the calibration bucket increases from 0.285D to 5D, the absolute value of the matching error first decreases and then increases, such that the optimal sand filling thickness is 3D. The output of the earth pressure cell increases with the decrease in sand particle size under the same load, and there is a significant difference between the theoretical calculation value and the experimental value of the matching error; aiming at this difference, an empirical formula is derived to reflect the ratio of the diameter of the induction diaphragm of the earth pressure cell to the maximum particle size of the sand filling. When the depth of the earth pressure cell inserted in the structure is “0”, the sensing surface is flush with the structure and the absolute value of the matching error is the smallest. Changes in the horizontal placement of the soil pressure cell in the calibration bucket result in significant differences in both the output and hysteresis of the calibration curve. To improve the measurement accuracy of soil pressure cells in scaled tests for applications such as in the retaining walls of excavation pits, tunnel outer surfaces, pile tops, pile ends, and soil pressure measurements in soil, calibration of the soil pressure cells is required before testing. Due to the considerable difference in the stress states of the soil pressure cell between granular media and uniform fluid media, calibration in soil is essential. During in-soil calibration, factors such as cyclic loading and unloading, soil compression, sand thickness and particle size, and the placement of the soil pressure cell all affect the calibration results. This paper primarily investigates the influence of these factors on the calibration curve and matching error. This study found that, as the sand thickness increases, the matching error decreases initially and then increases. Full article
(This article belongs to the Section Physical Sensors)
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<p>The earth pressure cell.</p>
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<p>Particle gradation curve of standard sand.</p>
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<p>Earth pressure cells in 4 groups of sand with different particle sizes.</p>
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<p>Soil calibration equipment for miniature earth pressure cells.</p>
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<p>Calibration Curve of the Soil Pressure Cell.</p>
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<p>Calibration curves of the earth pressure cell with different sand filling thickness.</p>
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<p>Relationship between matching error and sand filling thickness.</p>
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<p>Calibration curves of the earth pressure cell with different sand filling thickness.</p>
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<p>Curves of the matching error of the thickness of the upper and lower sand fills.</p>
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<p>Calibration curves of the earth pressure cell with different sand particle size.</p>
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<p>Relationship between matching error and sand particle size.</p>
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<p>Relationship curves of <math display="inline"><semantics> <mrow> <mi>f</mi> <mo stretchy="false">(</mo> <mrow> <mi>T</mi> <mo>/</mo> <mi>D</mi> </mrow> <mo stretchy="false">)</mo> </mrow> </semantics></math> and <math display="inline"><semantics> <mrow> <mi>T</mi> <mo>/</mo> <mi>D</mi> </mrow> </semantics></math>.</p>
Full article ">Figure 13
<p>Relationship curves of <math display="inline"><semantics> <mrow> <mi>f</mi> <mo stretchy="false">(</mo> <mrow> <mi>d</mi> <mo>/</mo> <mrow> <msub> <mi>d</mi> <mrow> <mi mathvariant="normal">s</mi> <mrow> <mi mathvariant="normal">m</mi> <mi mathvariant="normal">a</mi> <mi mathvariant="normal">x</mi> </mrow> </mrow> </msub> </mrow> </mrow> <mo stretchy="false">)</mo> </mrow> </semantics></math> and <math display="inline"><semantics> <mrow> <mi>d</mi> <mo>/</mo> <mrow> <msub> <mi>d</mi> <mrow> <mi mathvariant="normal">s</mi> <mrow> <mi mathvariant="normal">m</mi> <mi mathvariant="normal">a</mi> <mi mathvariant="normal">x</mi> </mrow> </mrow> </msub> </mrow> </mrow> </semantics></math>.</p>
Full article ">Figure 14
<p>Schematic diagram of changing the depth of the earth pressure cell inserted in the plexiglass plate.</p>
Full article ">Figure 15
<p>Calibration curves of the earth pressure cell with different inserted depths.</p>
Full article ">Figure 16
<p>Fitting relationship between matching error and inserted depth.</p>
Full article ">Figure 17
<p>Horizontal distribution of earth pressure cells in a free field of sand.</p>
Full article ">Figure 18
<p>Calibration curves of earth pressure boxes at different locations.</p>
Full article ">
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