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Search Results (2,282)

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20 pages, 4769 KiB  
Article
Assessment of MODIS and VIIRS Ice Surface Temperature Products over the Antarctic Ice Sheet
by Chenlie Shi, Ninglian Wang, Yuwei Wu, Quan Zhang, Carleen H. Reijmer and Paul C. J. P. Smeets
Remote Sens. 2025, 17(6), 955; https://doi.org/10.3390/rs17060955 - 7 Mar 2025
Viewed by 98
Abstract
The ice surface temperature (IST) derived from thermal infrared remote sensing is crucial for accurately monitoring ice or snow surface temperatures in the polar region. Generally, the remote sensing IST needs to be validated by the in situ IST to ensure its accuracy. [...] Read more.
The ice surface temperature (IST) derived from thermal infrared remote sensing is crucial for accurately monitoring ice or snow surface temperatures in the polar region. Generally, the remote sensing IST needs to be validated by the in situ IST to ensure its accuracy. However, due to the limited availability of in situ IST measurements, previous studies in the validation of remote sensing ISTs are scarce in the Antarctic ice sheet. This study utilizes ISTs from eight broadband radiation stations to assess the accuracy of the latest-released Moderate Resolution Imaging Spectroradiometer (MODIS) IST and Visible Infrared Imager Radiometer Suite (VIIRS) IST products, which were derived from two different algorithms, the Split-Window (SW-based) algorithm and the Temperature–Emissivity Separation (TES-based) algorithm, respectively. This study also explores the sources of uncertainty in the validation process. The results reveal prominent errors when directly validating remote sensing ISTs with the in situ ISTs, which can be attributed to incorrect cloud detection due to the similar spectral characteristics of cloud and snow. Hence, cloud pixels are misclassified as clear pixels in the satellite cloud mask during IST validation, which emphasizes the severe cloud contamination of remote sensing IST products. By using a cloud index (n) to remove the cloud contamination pixels in the remote sensing IST products, the overall uncertainties for the four products are about 2 to 3 K, with the maximum uncertainty (RMSE) reduced by 3.51 K and the bias decreased by 1.26 K. Furthermore, a progressive cold bias in the validation process was observed with decreasing temperature, likely due to atmospheric radiation between the radiometer and the snow surface being neglected in previous studies. Lastly, this study found that the cloud mask errors of satellites are more pronounced during the winter compared to that in summer, highlighting the need for caution when directly using remote sensing IST products, particularly during the polar night. Full article
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Figure 1
<p>The spatial distribution of the Antarctic ice sheet and eight broadband radiation stations. The stations with red color belong to the BSRN, green for the IMAU, and blue for Panda.</p>
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<p>Scatterplots of comparison results between MYD11 products and in situ IST for eight stations.</p>
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<p>Scatterplots of comparison results between MYD21 products and in situ IST for eight stations.</p>
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<p>Scatterplots of comparison results between VNP21 products and in situ IST for eight stations.</p>
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<p>Scatterplots of comparison results between VNP11 products and in situ IST for two stations.</p>
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<p>The color scatterplots of comparison results between the MYD11 and in situ IST. The color is closer to green, and the cloud index is closer to 1. The color is closer to yellow, and the cloud index is closer to 0.</p>
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<p>The validation accuracies between all sky and clear sky for the four products. (<b>a</b>–<b>d</b>) are for the MYD11, MYD21, VNP21, and VNP11, respectively.</p>
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<p>The assessment accuracies of the remote sensing IST with the in situ IST for different values of broadband emissivity. Green represents bias, and the blue color is the RMSE.</p>
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<p>The statistics of the numbers of each cloud index value for different stations.</p>
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<p>The number of different sky conditions (cloudy, mixed, and clear sky) in summer and winter for three IST products. Green color is the result in summer and blue color for winter; (<b>a</b>,<b>b</b>) are for MYD11; (<b>c</b>,<b>d</b>) are for MYD21; (<b>e</b>,<b>f</b>) are for VNP21.</p>
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<p>Comparison accuracies between TES-based IST and SW-based IST.</p>
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<p>The histogram of the temperature differences (ΔIST) between the in situ IST and remote sensing IST and the assessment accuracy: (<b>a</b>–<b>f</b>) are for the SW-based IST and (<b>g</b>–<b>l</b>) are for the TES-based IST.</p>
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16 pages, 5423 KiB  
Article
Optical Bacteria Recognition: Cross-Polarized Scattering
by Riccardo Pepino, Hamed Tari, Alessandro Bile, Arif Nabizada and Eugenio Fazio
Symmetry 2025, 17(3), 396; https://doi.org/10.3390/sym17030396 - 6 Mar 2025
Viewed by 173
Abstract
The rapid identification of bacteria is extremely important for controlling infections and enabling swift and effective action. Light scattering has proven to be a highly versatile technique for identifying bacteria, as it does not require long colony growth times. In this article, we [...] Read more.
The rapid identification of bacteria is extremely important for controlling infections and enabling swift and effective action. Light scattering has proven to be a highly versatile technique for identifying bacteria, as it does not require long colony growth times. In this article, we present a study on the use of cross-polarized optical scattering (CPS). Despite a relatively low scattering efficiency (10−5 to 10−6), working with cross-polarization enhances contrast by eliminating a highly intense background of scattered light. CPS has been applied to four bacteria, with three similar in shape. Moreover, two of them are Gram+ and two Gram-. The obtained images have been reduced in size down to a 16-bit images and camera noise has been added. Although bacteria are symmetrical in principle, in reality rotations of their orientation generate asymmetries in the CPS patterns that were exploited precisely to recognize and classify the different species. The classification of bacteria by a t-SNE algorithm in a reduced-dimension space shows that their features are grouped into specific clusters. However, such classification is not completely decisive due to partial cluster overlapping. Full article
(This article belongs to the Special Issue Symmetry/Asymmetry in Neuromorphic and Intelligent Photonics)
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<p>Schematic structure of Gram+ and Gram- bacteria.</p>
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<p>Three-dimensional models of individual bacteria. (<b>A</b>) <span class="html-italic">Salmonella enterica</span>. (<b>B</b>) <span class="html-italic">Vibrio cholerae</span>. (<b>C</b>) <span class="html-italic">Bacillus globigii</span>. (<b>D</b>) <span class="html-italic">Bacillus subtilis</span>.</p>
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<p>The simulations were performed by rotating the bacteria around the <span class="html-italic">x</span> and <span class="html-italic">z</span> axes to capture all possible orientations.</p>
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<p>CPS maps for <span class="html-italic">Salmonella</span> and <span class="html-italic">Vibrio cholerae</span> bacteria for rotations from 0° to 170° around the x-axis (blue color corresponds to zero light intensity).</p>
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<p>Cross-polarized optical scattering (CPS) images for <span class="html-italic">Salmonella</span> and <span class="html-italic">Bacilli</span> (<span class="html-italic">globigii</span> and <span class="html-italic">subtilis</span>) bacteria at normal incidence. Images display concentric elliptical structures (dashed lines), whose dimensions depend on size of bacteria (blue color corresponds to zero light intensity).</p>
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<p>Orientations (<b>a</b>,<b>c</b>) and corresponding CPS images (<b>b</b>,<b>d</b>) for <span class="html-italic">Vibrio cholerae</span> at angles around the <span class="html-italic">x</span>-axis of 0° and 45° (blue color corresponds to zero light intensity).</p>
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<p>Comparison of CPS images of the four bacteria for 90° rotations around the main axes <span class="html-italic">x</span> and <span class="html-italic">z</span> (blue color corresponds to zero light intensity).</p>
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<p><span class="html-italic">Salmonella</span> bacterium: x-angle of 45°, z-angle of 0°. Variation in CPS images for the refractive index values of the membranes as reported in <a href="#symmetry-17-00396-t003" class="html-table">Table 3</a>: (<b>A</b>) <math display="inline"><semantics> <mrow> <msub> <mrow> <mi>n</mi> </mrow> <mrow> <mi>c</mi> <mi>i</mi> <mi>t</mi> </mrow> </msub> <mo>=</mo> <mn>1.3883</mn> <mo>,</mo> <msub> <mrow> <mi>n</mi> </mrow> <mrow> <mi>m</mi> <mi>e</mi> <mi>m</mi> <mi>b</mi> </mrow> </msub> <mo>=</mo> <mn>1.43</mn> </mrow> </semantics></math>; (<b>B</b>) <math display="inline"><semantics> <mrow> <msub> <mrow> <mi>n</mi> </mrow> <mrow> <mi>c</mi> <mi>i</mi> <mi>t</mi> </mrow> </msub> <mo>=</mo> <mn>1.3883</mn> <mo>,</mo> <msub> <mrow> <mi>n</mi> </mrow> <mrow> <mi>m</mi> <mi>e</mi> <mi>m</mi> <mi>b</mi> </mrow> </msub> <mo>=</mo> <mn>1.45</mn> </mrow> </semantics></math>; (<b>C</b>) <math display="inline"><semantics> <mrow> <msub> <mrow> <mi>n</mi> </mrow> <mrow> <mi>c</mi> <mi>i</mi> <mi>t</mi> </mrow> </msub> <mo>=</mo> <mn>1.3935</mn> <mo>,</mo> <msub> <mrow> <mi>n</mi> </mrow> <mrow> <mi>m</mi> <mi>e</mi> <mi>m</mi> <mi>b</mi> </mrow> </msub> <mo>=</mo> <mn>1.43</mn> </mrow> </semantics></math>; and (<b>D</b>) <math display="inline"><semantics> <mrow> <msub> <mrow> <mi>n</mi> </mrow> <mrow> <mi>c</mi> <mi>i</mi> <mi>t</mi> </mrow> </msub> <mo>=</mo> <mn>1.3935</mn> <mo>,</mo> <msub> <mrow> <mi>n</mi> </mrow> <mrow> <mi>m</mi> <mi>e</mi> <mi>m</mi> <mi>b</mi> </mrow> </msub> <mo>=</mo> <mn>1.45</mn> </mrow> </semantics></math>. (blue color corresponds to zero light intensity).</p>
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<p>16-bit CPS images (all with same orientation at z = 0°). (blue color corresponds to zero light intensity).</p>
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<p>(<b>A</b>) TSNe normal images and (<b>B</b>) TSNe logarithmic scale images.</p>
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17 pages, 6231 KiB  
Article
Enhancing Image Reconstruction Method in High-Frequency Electric Field Visualization Systems Using a Polarized Light Image Sensor
by Kiyotaka Sasagawa, Ryoma Okada, Maya Mizuno, Hironari Takehara, Makito Haruta, Hiroyuki Tashiro and Jun Ohta
Sensors 2025, 25(5), 1596; https://doi.org/10.3390/s25051596 - 5 Mar 2025
Viewed by 165
Abstract
This paper introduces an image processing method, used to achieve uniform sensitivity across the imaging plane in a high-frequency electric field imaging system, that employs an electro-optical crystal and a polarization image sensor. The polarization pixels have two polarization directions, 0° and 90°, [...] Read more.
This paper introduces an image processing method, used to achieve uniform sensitivity across the imaging plane in a high-frequency electric field imaging system, that employs an electro-optical crystal and a polarization image sensor. The polarization pixels have two polarization directions, 0° and 90°, in pairs, and, conventionally, their difference is computed first. In contrast, this study proposes a method to separate each polarization image, perform pixel completion, and subsequently perform intensity correction. The proposed method was demonstrated to improve field distribution images acquired using 36 GHz and 30 GHz input signals for a microstrip line and patch antenna, respectively. From the measurement results of the microstrip line, the application of the proposed method reduced the electric field fluctuations on the line from 3.1 dB to 1.5 dB. This image-processing method can be applied sequentially during image acquisition, making it suitable for the real-time imaging of electric fields. Full article
(This article belongs to the Section Sensing and Imaging)
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<p>Schematic illustrating frequency conversion using optical heterodyne detection.</p>
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<p>Electric field imaging system setup.</p>
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<p>(<b>a</b>) Image and (<b>b</b>) block diagram of the polarization image sensor.</p>
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<p>Flowchart of image processing using the proposed method.</p>
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<p>Image of the microstrip line.</p>
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<p>Examples of processing results for each of the proposed image processing steps.</p>
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<p>Examples of field intensity and phase images on a microstrip line. (<b>a</b>) Result of simple processing used in the previous work. (<b>b</b>) Result of applying column interpolation processing. (<b>c</b>) Result of applying column interpolation and intensity correction processing. The number of images was 65,536.</p>
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<p>Intensity profile comparison on the solid blue lines shown in <a href="#sensors-25-01596-f007" class="html-fig">Figure 7</a>.</p>
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<p>Spectrum at a point on the microstrip line track in the center of the image, calculated using FFT.</p>
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<p>Comparison of images with different numbers of integrated images using signal extraction.</p>
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<p>Dependence of signal intensity and noise components calculated from 5 × 5 pixels on a microstrip line on the number of integrated copies. Signal intensity is plotted as extracted by software lock-in detection and FFT. The noise is calculated as the average of 20 points in the region slightly removed from the intermediate frequency. All values are normalized with respect to the value at 65,536 frames.</p>
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<p>(<b>a</b>) Image of the 30 GHz patch antenna. (<b>b</b>) Simulation result of the electric field distribution perpendicular to the patch anntena.</p>
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<p>Examples of field intensity and phase images on a 30 GHz patch antenna. (<b>a</b>) Result of simple processing method used in the previous work. (<b>b</b>) Result after applying column interpolation processing method. (<b>c</b>) Result after applying column interpolation and intensity correction processing method. The number of images is 10,000 frames.</p>
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<p>Electric field intensity profile on the line shown in <a href="#sensors-25-01596-f013" class="html-fig">Figure 13</a>.</p>
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<p>Results of continuous imaging and phase display of the electric field on a patch antenna rotating in phase with a 10 s period. The number of images is 360 frames.</p>
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23 pages, 10129 KiB  
Article
Smoothing Filter and Correction Factor for Two-Dimensional Electrical Resistivity Tomography and Time Domain-Induced Polarization Data Collected in Difficult Terrains to Improve Inversion Models
by Andrés Tejero-Andrade, Aide E. López-González, José M. Tejero-Andrade, René E. Chávez-Segura and Denisse L. Argote
Mathematics 2025, 13(5), 866; https://doi.org/10.3390/math13050866 - 5 Mar 2025
Viewed by 310
Abstract
When collecting data using ERT2D (2D electrical resistivity tomography) and TDIPT2D (2D time domain-induced polarization), different phenomena can occur, which can cause natural or anthropogenic noise, contaminating the data and making its processing, analysis, and interpretation difficult. Different techniques have been developed to [...] Read more.
When collecting data using ERT2D (2D electrical resistivity tomography) and TDIPT2D (2D time domain-induced polarization), different phenomena can occur, which can cause natural or anthropogenic noise, contaminating the data and making its processing, analysis, and interpretation difficult. Different techniques have been developed to eliminate or reduce these effects on the data, such as noise filtering or the development of new techniques to improve data collection in the field. In the present work, an iterative, weighted, least-squares filter was employed after voltage normalization using current and geometrical factor correction on data collected in rough topographic terrains. The selected filter basis function should be able to represent the natural behavior of the function to be filtered. Stationary or variable voltages in electrical prospecting decay with the inverse of the distance, which can be represented by an expansion in Legendre polynomials. On the other hand, uneven spacing of the electrodes leads to using the incorrect geometric factor, resulting in an error in the calculation of the electrical anomaly. The efficiency of the proposed technique was analyzed and tested with field examples using different filters and by comparing applying and not applying the proposed correction factor. The results indicated low RMS and L2-Norm errors, and better definition of the inverted resistivity image was obtained. For the TDIP data, a better correspondence between the inverted images of resistivity and chargeability was obtained. Full article
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<p>Data profile with the same spacing in the x-direction (<b>A</b>). A number of coefficients is assigned to each data point (<b>B</b>). The number of profile data points is indicated (<b>D</b>). For each number of coefficients, a data point <math display="inline"><semantics> <mrow> <msub> <mrow> <mi>P</mi> </mrow> <mrow> <mi>l</mi> </mrow> </msub> </mrow> </semantics></math> is obtained (<b>C</b>). The filter window moves from the first to the nth data point. The calculation of <math display="inline"><semantics> <mrow> <msub> <mrow> <mi mathvariant="bold-italic">s</mi> </mrow> <mrow> <mi mathvariant="bold-italic">j</mi> </mrow> </msub> </mrow> </semantics></math> will depend on the zone it is in (<b>E</b>).</p>
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<p>Flow chart of the proposed filtering method with three iterations.</p>
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<p><math display="inline"><semantics> <mrow> <msub> <mrow> <mi>r</mi> </mrow> <mrow> <mi>j</mi> </mrow> </msub> </mrow> </semantics></math> is the profile data or a function to be filtered; <math display="inline"><semantics> <mrow> <msub> <mrow> <mi>s</mi> </mrow> <mrow> <mi>j</mi> </mrow> </msub> </mrow> </semantics></math> is the filtered function; <math display="inline"><semantics> <mrow> <msubsup> <mrow> <mi>e</mi> </mrow> <mrow> <mi>j</mi> </mrow> <mrow> <mi mathvariant="normal">*</mi> </mrow> </msubsup> </mrow> </semantics></math> = (<math display="inline"><semantics> <mrow> <msub> <mrow> <mi>r</mi> </mrow> <mrow> <mi>j</mi> </mrow> </msub> <mo>−</mo> <msub> <mrow> <mi>s</mi> </mrow> <mrow> <mi>j</mi> </mrow> </msub> </mrow> </semantics></math>); <math display="inline"><semantics> <mrow> <msub> <mrow> <mi>e</mi> </mrow> <mrow> <mi>j</mi> </mrow> </msub> <mo>=</mo> <mstyle scriptlevel="0" displaystyle="true"> <mfrac> <mrow> <msubsup> <mrow> <mi>e</mi> </mrow> <mrow> <mi>j</mi> </mrow> <mrow> <mi mathvariant="normal">*</mi> </mrow> </msubsup> </mrow> <mrow> <msqrt> <mn>2</mn> </msqrt> </mrow> </mfrac> </mstyle> </mrow> </semantics></math>; and the relative error is <math display="inline"><semantics> <mrow> <msub> <mrow> <mi>R</mi> <mi>e</mi> </mrow> <mrow> <mi>j</mi> </mrow> </msub> </mrow> </semantics></math>= <math display="inline"><semantics> <mrow> <msqrt> <mn>2</mn> </msqrt> <mfenced separators="|"> <mrow> <mstyle scriptlevel="0" displaystyle="true"> <mfrac> <mrow> <msub> <mrow> <mi>r</mi> </mrow> <mrow> <mi>j</mi> </mrow> </msub> <mo>−</mo> <msub> <mrow> <mi>s</mi> </mrow> <mrow> <mi>j</mi> </mrow> </msub> </mrow> <mrow> <msub> <mrow> <mi>r</mi> </mrow> <mrow> <mi>j</mi> </mrow> </msub> <mo>+</mo> <msub> <mrow> <mi>s</mi> </mrow> <mrow> <mi>j</mi> </mrow> </msub> </mrow> </mfrac> </mstyle> </mrow> </mfenced> </mrow> </semantics></math>.</p>
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<p>Observed voltage and resistance when current and electrode spacing are not constant. A smoothed voltage is recovered after correction.</p>
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<p>Flow chart of the proposed method for filtering and correcting the resistivity and IP response.</p>
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<p>El Calvario (the Calvary) and its 2D Schlumberger–Wenner profile.</p>
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<p>ERT2D images of the Calvary. Seven resistivity sections were obtained from the following data: (<b>a</b>) raw resistivity data; (<b>b</b>) MA-filtered data; (<b>c</b>) MA-filtered and corrected data; (<b>d</b>) SG-filtered data; (<b>e</b>) SG-filtered and corrected data; (<b>f</b>) LSFLP-filtered data; and (<b>g</b>) LSFLP-filtered and corrected data.</p>
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<p>The hall response is centered at X = 22.5 m (rectangular box). Raw (original data), and moving average (MA)-, Savitzky–Golay (SG)-, and a least square weighted filter with Legendre polynomial (LSFLP)-filtered data.</p>
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<p>Hydrocarbon-contaminated sections. Seven resistivity sections were obtained: (<b>a</b>) raw resistivity section and (<b>b</b>) its IP section; (<b>c</b>) MA-filtered resistivity data section and (<b>d</b>) its IP section; (<b>e</b>) MA-filtered and corrected data and (<b>f</b>) its IP section; (<b>g</b>) SG-filtered data and (<b>h</b>) its IP section; (<b>i</b>) SG-filtered and corrected data and (<b>j</b>) its IP section; (<b>k</b>) LSFLP-filtered data and (<b>l</b>) its IP section; and (<b>m</b>) LSFLP-filtered and corrected data and (<b>n</b>) its IP section. Drill names 21, 25, MF21, and A11 are shown at the top of the figure in each column. Red represents high values; blue represents low values.</p>
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20 pages, 7366 KiB  
Article
Histogram of Polarization Gradient for Target Tracking in Infrared DoFP Polarization Thermal Imaging
by Jianguo Yang, Dian Sheng, Weiqi Jin and Li Li
Remote Sens. 2025, 17(5), 907; https://doi.org/10.3390/rs17050907 - 4 Mar 2025
Viewed by 147
Abstract
Division-of-focal-plane (DoFP) polarization imaging systems have demonstrated considerable promise in target detection and tracking in complex backgrounds. However, existing methods face challenges, including dependence on complex image preprocessing procedures and limited real-time performance. To address these issues, this study presents a novel histogram [...] Read more.
Division-of-focal-plane (DoFP) polarization imaging systems have demonstrated considerable promise in target detection and tracking in complex backgrounds. However, existing methods face challenges, including dependence on complex image preprocessing procedures and limited real-time performance. To address these issues, this study presents a novel histogram of polarization gradient (HPG) feature descriptor that enables efficient feature representation of polarization mosaic images. First, a polarization distance calculation model based on normalized cross-correlation (NCC) and local variance is constructed, which enhances the robustness of gradient feature extraction through dynamic weight adjustment. Second, a sparse Laplacian filter is introduced to achieve refined gradient feature representation. Subsequently, adaptive polarization channel correlation weights and the second-order gradient are utilized to reconstruct the degree of linear polarization (DoLP). Finally, the gradient and DoLP sign information are ingeniously integrated to enhance the capability of directional expression, thus providing a new theoretical perspective for polarization mosaic image structure analysis. The experimental results obtained using a self-developed long-wave infrared DoFP polarization thermal imaging system demonstrate that, within the same FBACF tracking framework, the proposed HPG feature descriptor significantly outperforms traditional grayscale {8.22%, 2.93%}, histogram of oriented gradient (HOG) {5.86%, 2.41%}, and mosaic gradient histogram (MGH) {27.19%, 18.11%} feature descriptors in terms of precision and success rate. The processing speed of approximately 20 fps meets the requirements for real-time tracking applications, providing a novel technical solution for polarization imaging applications. Full article
(This article belongs to the Special Issue Recent Advances in Infrared Target Detection)
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<p>Feature extraction methods for DoFP polarization images: (<b>a</b>) indirect processing method based on calibration and demosaicking; (<b>b</b>) direct processing method based on mosaic images.</p>
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<p>Schematic diagram of the HPG feature descriptor.</p>
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<p>Long-wave infrared DoFP polarization mosaic dataset.</p>
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<p>Vehicle target tracking results with occlusion under foggy night conditions.</p>
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<p>Vehicle target tracking results with scale variations under clear night conditions.</p>
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<p>Precision and success plots of different feature extraction methods.</p>
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<p>Precision and success plots of different tracking methods: TECF [<a href="#B31-remotesensing-17-00907" class="html-bibr">31</a>], EMCF [<a href="#B30-remotesensing-17-00907" class="html-bibr">30</a>], AutoTrack [<a href="#B29-remotesensing-17-00907" class="html-bibr">29</a>], ARCF [<a href="#B28-remotesensing-17-00907" class="html-bibr">28</a>], GFS-DCF [<a href="#B27-remotesensing-17-00907" class="html-bibr">27</a>], ECO [<a href="#B26-remotesensing-17-00907" class="html-bibr">26</a>], and SRDCF [<a href="#B25-remotesensing-17-00907" class="html-bibr">25</a>].</p>
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24 pages, 6487 KiB  
Article
Synchronous Atmospheric Correction of Wide-Swath and Wide-Field Remote Sensing Image from HJ-2A/B Satellite
by Honglian Huang, Yuxuan Wang, Xiao Liu, Rufang Ti, Xiaobing Sun, Zhenhai Liu, Xuefeng Lei, Jun Lin and Lanlan Fan
Remote Sens. 2025, 17(5), 884; https://doi.org/10.3390/rs17050884 - 1 Mar 2025
Viewed by 369
Abstract
The Chinese HuanjingJianzai-2 (HJ-2) A/B satellites are equipped with advanced sensors, including a Multispectral Camera (MSC) and a Polarized Scanning Atmospheric Corrector (PSAC). To address the challenges of atmospheric correction (AC) for the MSC’s wide-swath, wide-field images, this study proposes a pixel-by-pixel method [...] Read more.
The Chinese HuanjingJianzai-2 (HJ-2) A/B satellites are equipped with advanced sensors, including a Multispectral Camera (MSC) and a Polarized Scanning Atmospheric Corrector (PSAC). To address the challenges of atmospheric correction (AC) for the MSC’s wide-swath, wide-field images, this study proposes a pixel-by-pixel method incorporating Bidirectional Reflectance Distribution Function (BRDF) effects. The approach uses synchronous atmospheric parameters from the PSAC, an atmospheric correction lookup table, and a semi-empirical BRDF model to produce surface reflectance (SR) products through radiative, adjacency effect, and BRDF corrections. The corrected images showed significant improvements in clarity and contrast compared to pre-correction images, with minimum increases of 55.91% and 35.63%, respectively. Validation experiments in Dunhuang and Hefei, China, demonstrated high consistency between the corrected SR and ground-truth data, with maximum deviations below 0.03. For surface types not covered by ground measurements, comparisons with Sentinel-2 SR products yielded maximum deviations below 0.04. These results highlight the effectiveness of the proposed method in improving image quality and accuracy, providing reliable data support for applications such as disaster monitoring, water resource management, and crop monitoring. Full article
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<p>Schematic of synchronized detection between PSAC and MSC.</p>
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<p>Atmospheric correction flowchart for wide-swath and wide-field multispectral images.</p>
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<p>Matching results of AOD and CWV for MSC image. (<b>a</b>) Matched AOD distribution. (<b>b</b>) AOD distribution after linear interpolation. (<b>c</b>) Matched CWV distribution. (<b>d</b>) CWV distribution after linear interpolation.</p>
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<p>Comparison of pre- and post-atmospheric correction for HJ-2A satellite multispectral image of Beijing Daxing Airport, China. The red-marked and green-marked areas represent the selected regions for comparison and validation with Sentinel-2 data, as described in <a href="#sec4dot3-remotesensing-17-00884" class="html-sec">Section 4.3</a>. (CCD1, 14 November 2022; AOD = 0.446; CWV = 0.51 g/cm<sup>2</sup>). (<b>a</b>) Before atmospheric correction. (<b>b</b>) After atmospheric correction.</p>
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<p>Comparison of pre- and post-atmospheric correction for an HJ-2B satellite multispectral image of the Indian Plains region. The red-marked and green-marked areas represent the selected regions for comparison and validation with Sentinel-2 data, as described in <a href="#sec4dot3-remotesensing-17-00884" class="html-sec">Section 4.3</a>. (CCD3, 25 November 2022; AOD = 0.208; CWV = 0.96 g/cm<sup>2</sup>). (<b>a</b>) Before atmospheric correction. (<b>b</b>) After atmospheric correction.</p>
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<p>Comparison of pre- and post-atmospheric correction for HJ-2A satellite multispectral image of Xianning City, Hubei Province, China. The red-marked and green-marked areas represent the selected regions for comparison and validation with Sentinel-2 data, as described in <a href="#sec4dot3-remotesensing-17-00884" class="html-sec">Section 4.3</a>. (CCD3, 23 December 2022; AOD = 0.564; CWV = 0.45 g/cm<sup>2</sup>). (<b>a</b>) Before atmospheric correction. (<b>b</b>) After atmospheric correction.</p>
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<p>The contrast, clarity, and their improvements of the multispectral images of Daxing Airport, Beijing, China, before and after atmospheric correction from the HJ-2A satellite. (<b>a</b>) Contrast. (<b>b</b>) Clarity.</p>
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<p>The contrast, clarity, and their improvements of the multispectral images of the Indian Plains region, before and after atmospheric correction from the HJ-2B satellite. (<b>a</b>) Contrast. (<b>b</b>) Clarity.</p>
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<p>The contrast, clarity, and their improvements of the multispectral images of Xian Ning, Hubei Province, China, before and after atmospheric correction from the HJ-2A satellite. (<b>a</b>) Contrast. (<b>b</b>) Clarity.</p>
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<p>Comparison of pre- and post-atmospheric correction for an HJ-2B satellite multispectral image at the Dunhuang site in China. The red-marked area represents the ground measurement region at the Dunhuang site. (<b>a</b>) Before atmospheric correction. (<b>b</b>) After atmospheric correction.</p>
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<p>Comparison of pre- and post-atmospheric correction for HJ-2B satellite multispectral image at Northern high-reflectance site in Dunhuang, China. The red-marked area represents the ground measurement region at the high-reflectance site. (<b>a</b>) Before atmospheric correction. (<b>b</b>) After atmospheric correction.</p>
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<p>Comparison of pre- and post-atmospheric correction for an HJ-2A satellite multispectral image at the suburban area of Hefei, Anhui Province, China. The red-marked and blue-marked areas represent the ground measurement regions for the wheat field and river water, respectively. (<b>a</b>) Before atmospheric correction. (<b>b</b>) After atmospheric correction.</p>
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<p>The reflectance curve from the ground-based synchronized measurements. (<b>a</b>) Dunhuang, Gansu, China. (<b>b</b>) Hefei, Anhui, China.</p>
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<p>Comparison chart of ground-measured reflectance and atmospheric-corrected SR. (<b>a</b>) Dunhuang site (25 January 2021, HJ-2B). (<b>b</b>) High-reflectance site (25 January 2021, HJ-2B). (<b>c</b>) Wheat field (25 March 2021, HJ-2A). (<b>d</b>) River water (25 March 2021, HJ-2A).</p>
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20 pages, 9378 KiB  
Article
Ultra-Wideband Passive Polarization Conversion Metasurface for Radar Cross-Section Reduction Across C-, X-, Ku-, and K-Bands
by Xiaole Ren, Yunqing Liu, Zhonghang Ji, Qiong Zhang and Wei Cao
Micromachines 2025, 16(3), 292; https://doi.org/10.3390/mi16030292 - 28 Feb 2025
Viewed by 211
Abstract
In this study, we present a novel ultra-wideband passive polarization conversion metasurface (PCM) that integrates double V-shaped patterns with circular split-ring resonators. Operating without any external power supply or active components, this design effectively manipulates the polarization state of incident electromagnetic waves. Numerical [...] Read more.
In this study, we present a novel ultra-wideband passive polarization conversion metasurface (PCM) that integrates double V-shaped patterns with circular split-ring resonators. Operating without any external power supply or active components, this design effectively manipulates the polarization state of incident electromagnetic waves. Numerical and experimental results demonstrate that the proposed PCM can convert incident linear polarization into orthogonal states across a wide frequency range of 7.1–22.3 GHz, encompassing the C-, X-, Ku-, and K-bands. A fabricated prototype confirms that the polarization conversion ratio (PCR) exceeds 90% throughout the specified band. Furthermore, we explore an additional application of this passive metasurface for electromagnetic stealth, wherein it achieves over 10 dB of monostatic radar cross-section (RCS) reduction from 7.6 to 21.5 GHz. This broad effectiveness is attributed to strong electromagnetic resonances between the top and bottom layers, as well as the Fabry–Pérot cavity effect, as evidenced by detailed analyses of the underlying physical mechanisms and induced surface currents. These findings confirm the effectiveness of the proposed design and highlight its potential for future technological applications, including 6G communications, radar imaging, anti-interference measures, and electromagnetic stealth. Full article
(This article belongs to the Special Issue Microwave Passive Components, 2nd Edition)
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Figure 1
<p>Schematic of the polarization converter: (<b>a</b>) Three-dimensional view of the PCM unit cell and (<b>b</b>) 10 × 10 PCM unit cell.</p>
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<p>Proposed polarization converter unit cell: (<b>a</b>) front view and (<b>b</b>) side view.</p>
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<p>Reflection simulation results of this converter under normal x-polarization. (<b>a</b>) Amplitude and (<b>b</b>) phase of co- and cross-polarized reflection coefficients, (<b>c</b>) PCR, (<b>d</b>) polarization rotation angle ψ, and unwrapped phase difference δ for x-polarized normal incidence.</p>
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<p>Effects of geometric parameters on the co-polarized reflection coefficient: (<b>a</b>) V-shaped arm length <span class="html-italic">m</span>, (<b>b</b>) substrate thickness <span class="html-italic">d</span>, (<b>c</b>) distance from the V-shaped structure to the center <span class="html-italic">l</span>, and (<b>d</b>) opening angle α.</p>
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<p>(<b>a</b>) The proposed unit cell for polarization conversion incorporating electric field decomposition and (<b>b</b>) mirrored the proposed polarization converter unit cell.</p>
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<p>Unwrapped phase difference under normal incidence in the UV direction. The magnitude remains above 97% in the band of interest.</p>
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<p>Surface current (A/m) profiles of the metallic components and the ground plane in the proposed unit cell for a wave normally incident along the x-axis at frequencies of (<b>a</b>) 7.525 GHz, (<b>b</b>) 11.179 GHz, (<b>c</b>) 18.342 GHz, and (<b>d</b>) 21.648 GHz.</p>
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<p>PCM unit cell chessboard structure for RCS reduction: (<b>a</b>) Three-dimensional schematic and (<b>b</b>) front view.</p>
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<p>Electromagnetic wave vertical incidence simulated via CST Microwave Studio: (<b>a</b>) Comparison of RCS between the PCM chessboard structure and a metal plate of the same size and (<b>b</b>) RCS reduction curve.</p>
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<p>Three-dimensional RCS patterns at different frequencies: (<b>a</b>) 8 GHz, (<b>b</b>) 12 GHz, (<b>c</b>) 18 GHz, and (<b>d</b>) 20.5 GHz.</p>
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<p>Fabricated prototype and measurement environment. (<b>a</b>) Fabricated prototype of 20 × 20 array of proposed PCM and (<b>b</b>) photograph of the PCM measurement setup.</p>
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<p>Comparison of reflection simulation results and experimental measurements of the PCM.</p>
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<p>Simulated PCR via CST Microwave Studio under various incident conditions. The results for x- and y-polarized incidences are identical, indicating polarization insensitivity along the principal axes. (<b>a</b>) PCR at different polarization angles under normal incidence and (<b>b</b>) PCR under oblique incidence with varying angles.</p>
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30 pages, 8461 KiB  
Article
Layer-by-Layer Multifractal Scanning of Optically Anisotropic Architectonics of Blood Plasma Films: Fundamental and Applied Aspects
by Alexander Ushenko, Natalia Pavlyukovich, Oksana Khukhlina, Olexander Pavlyukovich, Mykhaylo Gorsky, Iryna Soltys, Alexander Dubolazov, Yurii Ushenko, Olexander Salega, Ivan Mikirin, Jun Zheng, Zhebo Chen and Lin Bin
Photonics 2025, 12(3), 215; https://doi.org/10.3390/photonics12030215 - 28 Feb 2025
Viewed by 169
Abstract
This study focuses on the topographic structure of optical anisotropy maps (theziograms) of dehydrated blood plasma films (facies) to identify and utilize markers for diagnosing self-similarity (multifractality) in the birefringence parameters of supramolecular protein networks. The research is based on the Jones-matrix analytical [...] Read more.
This study focuses on the topographic structure of optical anisotropy maps (theziograms) of dehydrated blood plasma films (facies) to identify and utilize markers for diagnosing self-similarity (multifractality) in the birefringence parameters of supramolecular protein networks. The research is based on the Jones-matrix analytical framework, which describes the formation of polarization-structural speckle fields in polycrystalline blood plasma facies. In the proposed model, algorithms were developed to relate the real and imaginary parts of the complex elements of the Jones matrix to the theziograms of linear and circular birefringence. To experimentally implement these algorithms, a novel optical technology was introduced for polarization-interference registration and phase scanning of the laser speckle field of blood plasma facies. The laser-based Jones-matrix layer-by-layer theziography relies on polarization filtration and the digital recording of interference patterns from microscopic images of blood plasma facies. This process includes digital 2D Fourier reconstruction and phase-by-phase scanning of the object field of complex amplitudes, enabling the acquisition of phase sections of laser polarization-structural speckle field components scattered with varying multiplicities. Jones-matrix images of supramolecular networks, along with their corresponding theziograms of linear and circular birefringence, were obtained for each phase plane. The experimental data derived from laser layer-by-layer Jones-matrix theziography were quantitatively analyzed using two complementary approaches: statistical analysis (central moments of the 1st to 4th orders) and multifractal analysis (spectra of fractal dimension distributions). As a result, the most sensitive markers—namely asymmetry and kurtosis—were identified, highlighting changes in the statistical and scale self-similar structures of the theziograms of linear and circular birefringence in blood plasma facies. The practical aspect of this work is to evaluate the diagnostic potential of the Jones-matrix theziography method for identifying and differentiating changes in the birefringence of supramolecular networks in blood plasma facies caused by the long-term effects of COVID-19. For this purpose, a control group (healthy donors) and three experimental groups of patients, confirmed to have had COVID-19 one-to-three years prior, were formed. Within the framework of evidence-based medicine, the operational characteristics of the method—sensitivity, specificity, and accuracy—were assessed. The method demonstrated excellent accuracy in the differential diagnosis of the long-term effects of COVID-19. This was achieved by statistically analyzing the spectra of fractal dimensions of Jones-matrix theziograms reconstructed in the phase plane of single scattering within the volume of blood plasma facies. Full article
(This article belongs to the Special Issue Emerging Trends in Polarization Optics for Biomedical Applications)
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<p>Polarization-interference optical scheme for Jones-matrix mapping. 1—He-Ne laser; 2—collimator—“O”; 3, 11—beam splitters—“BS”; 4, 5—mirrors—“M”; 7, 10, 13—polarizer’s “P”; 6, 9—quarter wave plates—“QP”; 8—object; 12—polarization objective—“O”; 14—digital camera—“CCD”; 15—personal computer—“PC”.</p>
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<p>Jones-matrix reconstructed integral (<b>a</b>,<b>b</b>), layer-by-layer (<b>c</b>–<b>f</b>) theziograms of phase anisotropy <math display="inline"><semantics> <mrow> <mi>J</mi> <mi>T</mi> <mo>=</mo> <mfenced separators="|"> <mrow> <mtable> <mtr> <mtd> <mrow> <mi>L</mi> <mi>B</mi> <mfenced separators="|"> <mrow> <mrow> <mrow> <mi mathvariant="italic">Re</mi> </mrow> <mo>⁡</mo> <mrow> <msub> <mrow> <mi>j</mi> </mrow> <mrow> <mi>p</mi> <mi>k</mi> </mrow> </msub> </mrow> </mrow> </mrow> </mfenced> </mrow> </mtd> </mtr> <mtr> <mtd> <mrow> <mi>C</mi> <mi>B</mi> <mfenced separators="|"> <mrow> <mrow> <mrow> <mi mathvariant="italic">Re</mi> </mrow> <mo>⁡</mo> <mrow> <msub> <mrow> <mi>j</mi> </mrow> <mrow> <mi>p</mi> <mi>k</mi> </mrow> </msub> </mrow> </mrow> </mrow> </mfenced> </mrow> </mtd> </mtr> </mtable> </mrow> </mfenced> </mrow> </semantics></math>․coordinate distributions of linear birefringence (<span class="html-italic">LB</span>) (<b>a</b>,<b>c</b>,<b>e</b>) and circular birefringence (<span class="html-italic">CB</span>) (<b>b</b>,<b>d</b>,<b>f</b>) of the supramolecular networks of the <b>BPF1</b> sample.</p>
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<p>Jones-matrix reconstructed integral (<b>a</b>,<b>b</b>), layer-by-layer (<b>c</b>–<b>f</b>) theziograms of phase anisotropy <math display="inline"><semantics> <mrow> <mi>T</mi> <mo>=</mo> <mfenced separators="|"> <mrow> <mtable> <mtr> <mtd> <mrow> <mi>L</mi> <mi>B</mi> <mfenced separators="|"> <mrow> <mrow> <mrow> <mi mathvariant="italic">Re</mi> </mrow> <mo>⁡</mo> <mrow> <msub> <mrow> <mi>j</mi> </mrow> <mrow> <mi>p</mi> <mi>k</mi> </mrow> </msub> </mrow> </mrow> </mrow> </mfenced> </mrow> </mtd> </mtr> <mtr> <mtd> <mrow> <mi>C</mi> <mi>B</mi> <mfenced separators="|"> <mrow> <mrow> <mrow> <mi mathvariant="italic">Re</mi> </mrow> <mo>⁡</mo> <mrow> <msub> <mrow> <mi>j</mi> </mrow> <mrow> <mi>p</mi> <mi>k</mi> </mrow> </msub> </mrow> </mrow> </mrow> </mfenced> </mrow> </mtd> </mtr> </mtable> </mrow> </mfenced> </mrow> </semantics></math>․coordinate distributions of linear birefringence (<span class="html-italic">LB</span>) (<b>a</b>,<b>c</b>,<b>e</b>) and circular birefringence (<span class="html-italic">CB</span>) (<b>b</b>,<b>d</b>,<b>f</b>) of the supramolecular networks of the <b>BPF2</b> sample.</p>
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<p>Power spectra logarithmic dependences (fragments (<b>a</b>,<b>d</b>)); wavelet transform module maxima skeletons (fragments (<b>b</b>,<b>e</b>)) and multifractal spectra of <b>BPF1</b> phase anisotropy theziograms (fragments (<b>c</b>,<b>f</b>)).</p>
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<p>Power spectra logarithmic dependences (fragments (<b>a</b>,<b>d</b>)); wavelet transform modules maxima skeletons (fragments (<b>b</b>,<b>e</b>)) and multifractal spectra of <b>BPF2</b> phase anisotropy theziograms (fragments (<b>c</b>,<b>f</b>)).</p>
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<p>Power spectra logarithmic dependences (fragments (<b>a</b>,<b>d</b>)); wavelet transform module maxima skeletons (fragments (<b>b</b>,<b>e</b>)) and multifractal spectra of <b>BPF1</b> phase anisotropy theziograms <math display="inline"><semantics> <mrow> <msup> <mrow> <mi>J</mi> <mi>T</mi> </mrow> <mrow> <mi>L</mi> <mi>B</mi> <mo>;</mo> <mi>C</mi> <mi>B</mi> </mrow> </msup> <mfenced separators="|"> <mrow> <msub> <mrow> <mi>φ</mi> </mrow> <mrow> <mi>k</mi> </mrow> </msub> <mo>=</mo> <mstyle scriptlevel="0" displaystyle="true"> <mfrac bevelled="true"> <mrow> <mi>π</mi> </mrow> <mrow> <mn>4</mn> </mrow> </mfrac> </mstyle> </mrow> </mfenced> </mrow> </semantics></math> (fragments (<b>c</b>,<b>f</b>)).</p>
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<p>Power spectra logarithmic dependences (fragments (<b>a</b>,<b>d</b>)); wavelet transform module maxima skeletons (fragments (<b>b</b>,<b>e</b>)) and multifractal spectra of <b>BPF1</b> phase anisotropy theziograms <math display="inline"><semantics> <mrow> <msup> <mrow> <mi>J</mi> <mi>T</mi> </mrow> <mrow> <mi>L</mi> <mi>B</mi> <mo>;</mo> <mi>C</mi> <mi>B</mi> </mrow> </msup> <mfenced separators="|"> <mrow> <msub> <mrow> <mi>φ</mi> </mrow> <mrow> <mi>k</mi> </mrow> </msub> <mo>=</mo> <mstyle scriptlevel="0" displaystyle="true"> <mfrac bevelled="true"> <mrow> <mi>π</mi> </mrow> <mrow> <mn>8</mn> </mrow> </mfrac> </mstyle> </mrow> </mfenced> </mrow> </semantics></math> (fragments (<b>c</b>,<b>f</b>)).</p>
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<p>Power spectra logarithmic dependences (fragments (<b>a</b>,<b>d</b>)); wavelet transform module maxima skeletons (fragments (<b>b</b>,<b>e</b>)) and multifractal spectra of <b>BPF2</b> phase anisotropy theziograms <math display="inline"><semantics> <mrow> <msup> <mrow> <mi>J</mi> <mi>T</mi> </mrow> <mrow> <mi>L</mi> <mi>B</mi> <mo>;</mo> <mi>C</mi> <mi>B</mi> </mrow> </msup> <mfenced separators="|"> <mrow> <msub> <mrow> <mi>φ</mi> </mrow> <mrow> <mi>k</mi> </mrow> </msub> <mo>=</mo> <mstyle scriptlevel="0" displaystyle="true"> <mfrac bevelled="true"> <mrow> <mi>π</mi> </mrow> <mrow> <mn>4</mn> </mrow> </mfrac> </mstyle> </mrow> </mfenced> </mrow> </semantics></math> (fragments (<b>c</b>,<b>f</b>)).</p>
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<p>Power spectra logarithmic dependences (fragments (<b>a</b>,<b>d</b>)); wavelet transform module maxima skeletons (fragments (<b>b</b>,<b>e</b>)) and multifractal spectra of <b>BPF2</b> phase anisotropy theziograms <math display="inline"><semantics> <mrow> <msup> <mrow> <mi>J</mi> <mi>T</mi> </mrow> <mrow> <mi>L</mi> <mi>B</mi> <mo>;</mo> <mi>C</mi> <mi>B</mi> </mrow> </msup> <mfenced separators="|"> <mrow> <msub> <mrow> <mi>φ</mi> </mrow> <mrow> <mi>k</mi> </mrow> </msub> <mo>=</mo> <mstyle scriptlevel="0" displaystyle="true"> <mfrac bevelled="true"> <mrow> <mi>π</mi> </mrow> <mrow> <mn>8</mn> </mrow> </mfrac> </mstyle> </mrow> </mfenced> </mrow> </semantics></math> (fragments (<b>c</b>,<b>f</b>)).</p>
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14 pages, 4067 KiB  
Article
Spherical Amides with C3 Symmetry: Improved Synthetic Approach and Structural/Optical Analysis
by Daiki Koike, Hyuma Masu, Haruka Uno, Shoko Kikkawa, Hidemasa Hikawa and Isao Azumaya
Molecules 2025, 30(5), 1074; https://doi.org/10.3390/molecules30051074 - 26 Feb 2025
Viewed by 137
Abstract
A spherical amide with C3 symmetry was synthesized by a one-step cyclization reaction using triphenylphosphine and hexachloroethane as coupling reagents. This method enabled synthesis of N-benzyl and N-allyl derivatives, which could not be obtained by the previously reported method. The [...] Read more.
A spherical amide with C3 symmetry was synthesized by a one-step cyclization reaction using triphenylphosphine and hexachloroethane as coupling reagents. This method enabled synthesis of N-benzyl and N-allyl derivatives, which could not be obtained by the previously reported method. The optical resolution of each compound was measured, and their electronic circular dichroism spectra revealed that they were mirror images. The high structural symmetry resulted in a higher Δε (molar absorption difference against right or left circular polarization: εLεR value compared to that of another structural isomer synthesized previously. The absolute structure of the enantiopure crystal of the N-benzyl derivative was determined using the Flack parameter obtained by X-ray crystallographic analysis. Full article
(This article belongs to the Section Organic Chemistry)
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Graphical abstract

Graphical abstract
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<p>(<b>a</b>) Equilibrium of racemization of <span class="html-italic">m</span>-calix[3]amide. When <span class="html-italic">m</span>-calix[3]amide and a benzene ring are connected through amide bonds, spherical molecule <b>1</b>, in which the racemization is suppressed, is obtained. (<b>b</b>) Four structural isomers (<b>1</b>–<b>4</b>) of the spherical amide. Each component is colored as follows. a: Red: trimesic acid; b: green: 3,5-bis(alkylamino)benzoic acid; c: black: 3-(alkylamino)isophthalic acid; d: blue: 1,3,5-tri(alkylamino)benzene. R: alkyl group.</p>
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<p>Synthetic routes to spherical amide <b>1</b> [<a href="#B37-molecules-30-01074" class="html-bibr">37</a>].</p>
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<p><sup>1</sup>H NMR spectrum of <b>1b</b>. The benzene or benzyl units in the structure are indicated as follows. Red: trimesic acid (core); green: 3,5-diamino benzoic acid (core); pink: <span class="html-italic">N</span>-benzyl groups at the side; blue: <span class="html-italic">N</span>-benzyl groups at the bottom.</p>
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<p>ECD spectra of <b>1a</b> (<span class="html-italic">N</span>-ethyl), <b>1b</b> (<span class="html-italic">N</span>-benzyl), and <b>1c</b> (<span class="html-italic">N</span>-allyl) in acetonitrile solution. The sign of (+) or (−) was determined by optical rotation at 589 nm in acetonitrile solution.</p>
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<p>Comparison of ECD spectra of (<b>a</b>) <b>1a</b> and <b>2a</b> (<span class="html-italic">N</span>-ethyl), (<b>b</b>) <b>1b</b> and <b>2b</b> (<span class="html-italic">N</span>-benzyl), and (<b>c</b>) <b>2a</b> and <b>2b</b> in acetonitrile solution. (<b>d</b>) UV absorption spectra of the same compounds in acetonitrile solution.</p>
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<p>Crystal structures of chiral (+)-1b. (<b>a</b>) Molecular structure. (<b>b</b>) Superimposed structures. Pink: molecule A with benzene rings numbered C1–C6; pale blue: molecule B with benzene rings numbered C101–C106.</p>
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<p>Molecular arrangements in the crystals of (+)-1a (R = Et, CCDC code: LOJMIH) and (+)-1b (R = Bn). (<b>a</b>) Crystal structures of (+)-1a. The view is along the <span class="html-italic">a</span> axis. Green: molecule C; pale green: molecule D. (<b>b</b>) Crystal structures of (+)-1b. The view is along the <span class="html-italic">c</span> axis. Pink: molecule A; pale blue: molecule B.</p>
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<p>Synthesis of isomer <b>1</b> with ethyl groups (<b>1a</b>).</p>
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<p>Synthesis of monomer (<b>a</b>) <b>8b</b>: <span class="html-italic">N</span>-benzyl and (<b>b</b>) <b>8c</b>: <span class="html-italic">N</span>-allyl.</p>
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<p>Synthesis of spherical amide <b>1b</b> and <b>1c</b>.</p>
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21 pages, 9107 KiB  
Article
Body Temperature Detection of Group-Housed Pigs Based on the Pairing of Left and Right Ear Roots in Thermal Images
by Rong Xiang, Yi Zhang, Hongjian Lin, Yingchun Fu, Xiuqin Rao, Jinming Pan and Chenghao Pan
Animals 2025, 15(5), 642; https://doi.org/10.3390/ani15050642 - 22 Feb 2025
Viewed by 222
Abstract
Body temperature is a critical indicator of pig health. This study proposes a non-contact method for detecting body temperature in group-housed pigs by extracting temperature data from thermal images of ear roots. Thermal images in the drinking trough area were captured using a [...] Read more.
Body temperature is a critical indicator of pig health. This study proposes a non-contact method for detecting body temperature in group-housed pigs by extracting temperature data from thermal images of ear roots. Thermal images in the drinking trough area were captured using a thermal camera, with real-time data transmitted to a monitoring room via optical fibers. The YOLO v11m-OBB model was utilized to detect the ear root areas with oriented bounding boxes, while a novel algorithm, the two-stage left and right ear root pairing algorithm (YOLO TEPA-OBB), paired the ear roots of individual pigs using center distance clustering and angular relationships in a polar coordinate system. The maximum temperature of the ear roots was extracted to represent the body temperature. Experimental results based on 749 ear roots show that the YOLO TEPA-OBB achieves 98.7% precision, 98.4% recall, and 98.7% mean average precision (mAP) in detecting ear roots, with an ear root pairing accuracy of 98.1%. The Pearson correlation coefficient (r) between predicted and reference temperatures is 0.989, with a mean bias of 0.014 °C and a standard deviation of 0.103 °C. This research facilitates real-time body temperature monitoring and precise health management for group-housed pigs. Full article
(This article belongs to the Section Pigs)
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<p>Thermal image collection system for pigs. <b>a</b>. Thermal camera; <b>b</b>. fiber-optic transceiver; <b>c</b>. temperature and humidity logger; <b>d</b>. switch; <b>e</b>. DVR; <b>f</b>. captured images.</p>
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<p>Flowchart for individual temperature detection of group-housed pigs. (‘1–4’ refers to the ear root bounding boxes, and “➀–➁” refers to the number of ear root pairs.).</p>
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<p>YOLO v11m-OBB network architecture.</p>
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<p>TEPA algorithm workflow. (<b>a</b>) Center distance calculation; (<b>b</b>) rough pairing; (<b>c</b>) calculation of base and outermost points; (<b>d</b>) extraction of outermost lines and intersection points; (<b>e</b>) left and right ear classification; (<b>f</b>) re-pairing. (‘1–4’ refers to the ear root bounding boxes, and “①–②” refers to the number of ear root pairs.).</p>
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<p>Visualization of pig body temperature distribution. (<b>a</b>) Original thermal image; (<b>b</b>) 3D body temperature distribution (top view); (<b>c</b>) 3D body temperature distribution (side view); (<b>d</b>) 3D body temperature distribution (3D view).</p>
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<p>Extraction of reference and predicted values. (<b>a</b>) Reference value extraction (ear root line in bold black); (<b>b</b>) predicted value extraction.</p>
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<p>Comparison of ear root detection model results. (<b>a</b>) Result of YOLOv8 nano-OBB; (<b>b</b>) result of YOLOv8 medium-OBB; (<b>c</b>) result of YOLOv11 nano-OBB; (<b>d</b>) result of YOLOv11 medium-OBB.</p>
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<p>Results of training and validation sets. (<b>a</b>) Training—bounding box loss; (<b>b</b>) training—classification loss; (<b>c</b>) validation—precision; (<b>d</b>) validation—recall; (<b>e</b>) validation—bounding box loss; (<b>f</b>) validation—classification loss; (<b>g</b>) validation—mean average precision at 50%; (<b>h</b>) validation—mean average precision at 50–95%.</p>
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<p>Center distance distribution between left and right ear root bounding boxes.</p>
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<p>Typical left and right ear root pairing errors.</p>
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<p>Statistical results of body temperature detection. (<b>a</b>) Bias histogram; (<b>b</b>) hypothesis test for bias.</p>
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<p>Statistical results of body temperature detection. (<b>a</b>) Bias histogram; (<b>b</b>) hypothesis test for bias.</p>
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39 pages, 21233 KiB  
Article
Sex-Specific Adaptations in Alzheimer’s Disease and Ischemic Stroke: A Longitudinal Study in Male and Female APPswe/PS1dE9 Mice
by Klara J. Lohkamp, Nienke Timmer, Gemma Solé Guardia, Justin Shenk, Vivienne Verweij, Bram Geenen, Pieter J. Dederen, Lieke Bakker, Cansu Egitimci, Rengin Yoldas, Minou Verhaeg, Josine Kothuis, Desirée Nieuwenhuis, Maximilian Wiesmann and Amanda J. Kiliaan
Life 2025, 15(3), 333; https://doi.org/10.3390/life15030333 - 21 Feb 2025
Viewed by 305
Abstract
The long-term impact of stroke on Alzheimer’s disease (AD) progression, particularly regarding sex-specific differences, remains unknown. Using a longitudinal study design, we investigated transient middle cerebral artery occlusion in 3.5-month-old APPswe/PS1dE9 (APP/PS1) and wild-type mice. In vivo, we assessed behavior, [...] Read more.
The long-term impact of stroke on Alzheimer’s disease (AD) progression, particularly regarding sex-specific differences, remains unknown. Using a longitudinal study design, we investigated transient middle cerebral artery occlusion in 3.5-month-old APPswe/PS1dE9 (APP/PS1) and wild-type mice. In vivo, we assessed behavior, cerebral blood flow (CBF), and structural integrity by neuroimaging, as well as post-mortem myelin integrity (polarized light imaging, PLI), neuroinflammation, and amyloid beta (Aβ) deposition. APP/PS1 mice exhibited cognitive decline, white matter degeneration (reduced fractional anisotropy (FA) via diffusion tensor imaging (DTI)), and decreased myelin density via PLI. Despite early hypertension, APP/PS1 mice showed only sporadic hypoperfusion. Cortical thickening and hippocampal hypertrophy likely resulted from Aβ accumulation and neuroinflammation. Stroke-operated mice retained cognition despite cortical thinning and hippocampal atrophy due to cerebrovascular adaptation, including increased CBF in the hippocampus and thalamus. Stroke did not worsen AD pathology, nor did AD exacerbate stroke outcomes. Sex differences were found: female APP/PS1 mice had more severe Aβ deposition, hyperactivity, lower body weight, and reduced CBF but less neuroinflammation, suggesting potential neuroprotection. These findings highlight white matter degeneration and Aβ pathology as key drivers of cognitive decline in AD, with stroke-related deficits mitigated by (cerebro)vascular adaptation. Sex-specific therapies are crucial for AD and stroke. Full article
(This article belongs to the Section Medical Research)
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Figure 1

Figure 1
<p>Overview of the study design. This longitudinal study investigated the impact of stroke and Alzheimer’s disease on cognitive impairment, emphasizing sex differences. Male and female WT and APP<sub>swe</sub>/PS1<sub>dE9</sub> (APP/PS1) mice (3-month-old) were included. Baseline physiological parameters (body weight (BW) and systolic blood pressure (SBP)) were monitored. At 3.5 months of age, mice underwent either right transient middle cerebral artery occlusion (tMCAO) or sham surgery, resulting in eight groups: (1) male WT sham, (2) male WT stroke, (3) male APP/PS1 sham, (4) male APP/PS1 stroke, (5) female WT sham, (6) female WT stroke, (7) female APP/PS1 sham, and (8) female APP/PS1 stroke. Body weight and systolic blood pressure were monitored monthly post-stroke. Walking patterns of each mouse were individually monitored 24/7 for eight months using digital ventilated cages (DVCs). Magnetic resonance imaging (MRI) was conducted at 0.5, 4, and 8 months post-stroke. At 12 months of age (8 months post-stroke), spatial learning and memory were assessed using the Morris water maze (MWM) test. After the final neuroimaging session, mice were sacrificed, and brains were collected for post-mortem analysis, including immunohistochemical stainings, polarized light imaging, and biochemistry.</p>
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<p>Monthly (<b>A</b>,<b>B</b>) body weight and (<b>C</b>) systolic blood pressure (SBP) measurements were performed from baseline over a period of 8 months, following either sham or stroke surgery in both male and female WT and APP/PS1 mice. (<b>A</b>) Prior to stroke (baseline), at 3.5 months of age, female mice had a lower body weight than male mice (a: male vs. female; male n = 42, female n = 49). Across the 8 months post-surgery, male WT and male APP/PS1 mice consistently gained weight. Female WT and APP/PS1 mice exhibited less weight gain than males, with female mice generally being lighter than males (b: female WT vs. male WT, c: male APP/PS1 vs. female APP/PS1). From month 4, male APP/PS1 mice outweighed male WT mice, while female APP/PS1 mice were lighter than female WT mice, especially in the last two months (d: male APP/PS1 vs. male WT, e: female APP/PS1 vs. female WT; male WT n = 27, male APP/PS1 n = 15, female WT n = 29–30, female APP/PS1 n = 19). (<b>B</b>) From 6 months post-stroke onward, WT stroke mice displayed lower body weight compared to the control group that underwent sham surgery (f: WT stroke vs. WT sham; WT sham n = 29, WT stroke n = 27–28, APP/PS1 sham n = 17, APP/PS1 stroke n = 17). (<b>C</b>) At baseline, APP/PS1 mice had higher systolic blood pressure (SBP) than WT mice (g: APP/PS1 vs. WT). Post-surgery, APP/PS1 mice continued to exhibit higher SBP compared to their WT counterparts (WT n = 42–51, APP/PS1 n = 25–34). Furthermore, we monitored the distance the animals walked in their home cages during both day and night. During daytime, we observed that (<b>D</b>) female mice walked more than males (male n = 27–32, female n = 32–42), (<b>E</b>) stroke-operated mice walked more than sham-operated mice (sham n = 29–33, stroke n = 30–41), and (<b>F</b>) APP/PS1 mice walked more than WT mice (WT n = 39–45, APP/PS1 n = 20–29). (<b>G</b>) During nighttime, female WT mice walked more than male WT mice (b: female WT vs. male WT), female APP/PS1 mice walked more than male APP/PS1 mice (c: male APP/PS1 vs. female APP/PS1), male APP/PS1 walked more than male WT mice (d: male APP/PS1 vs. male WT), and female APP/PS1 walked more than female WT (e: female APP/PS1 vs. female WT; male WT n = 23–24, male APP/PS1 n = 10–14, female WT n = 22–27, female APP/PS1 n = 13–18). (<b>H</b>) Moreover, stroke-operated mice consistently walked longer distances than sham-operated mice (sham n = 29–33, stroke n = 30–41). Data are presented as mean ± SEM. Significance is denoted as * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001.</p>
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<p>Assessment of spatial learning and memory in the Morris water maze (MWM). This figure illustrates the results of the Morris water maze test performed on male and female, sham- or stroke-induced APP/PS1 and WT mice, 8 months post-surgery at 12 months of age. Results from the 4-day acquisition phase, assessing spatial learning abilities, are depicted in the upper panel: (<b>A</b>) The escape latency, a measure of spatial learning ability, decreased in WT and APP/PS1 mice. However, APP/PS1 mice exhibited longer times to find the hidden platform compared to WT mice, a finding indicative of AD-related decline in spatial learning (WT n = 55, APP/PS1 n = 25). (<b>B</b>) Cognitive scores increased but were lower in APP/PS1 mice compared to WT mice (WT n = 55, APP/PS1 n = 25). (<b>C</b>) Moreover, APP/PS1 mice swam longer distances than WT mice (WT n = 55, APP/PS1 n = 25). (<b>D</b>) In terms of sex differences, female mice swam further distances than male mice (male n = 38, female n = 42). (<b>E</b>) Male WT mice exhibited a decrease in swim velocity over time, while female WT mice swam faster than their male counterparts on days 3 and 4. On day 4, male APP/PS1 mice swam faster than WT males (male WT n = 26, male APP/PS1 n = 12, female WT n = 29, female APP/PS1 n = 13) (b: female WT vs. male WT, d: male APP/PS1 vs. male WT). Results of the probe trial during its first 30 s, assessing spatial memory abilities, are shown in the lower panel. Female mice that underwent stroke surgery (<b>F</b>) swam shorter distances and (<b>G</b>) swam slower than their male counterparts. Conversely, male stroke mice exhibited an increased (<b>H</b>) swim distance and (<b>F</b>) swim velocity compared to male sham-operated mice (male sham n = 16, male stroke n = 16, female sham n = 20, female stroke n = 17). (<b>H</b>) Additionally, APP/PS1 mice maintained a greater distance from the former platform location than WT mice, suggesting a diminished spatial memory associated with AD (WT n = 46, APP/PS1 n = 23). Data are presented as mean ± SEM. Significance is denoted as * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001.</p>
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<p>Longitudinal analysis of cortical thickness and hippocampal volume in male and female WT and APP/PS1 mice assessed at 0.5, 4, and 8 months post-stroke or -sham surgery. (<b>A</b>) Cortical thickness in the right (surgery-affected) hemisphere was consistently smaller than in the left hemisphere across all timepoints (right n = 86–90, left n = 86–90). (<b>B</b>) Overall, stroke-operated animals displayed a thinner right cortex compared to sham-operated animals (sham n = 44–45, stroke n = 42–45). (<b>C</b>) Over the 8-month post-surgery period, the right hemisphere’s cortex was consistently thicker in female compared to male mice (male n = 39–42, female n = 47–49). (<b>D</b>) Female mice exhibited a thicker cortex than male mice at 0.5 and 4 months post-surgery (male n = 78–84, female n = 94–98). (<b>E</b>) APP/PS1 mice had a thicker right cortex compared to WT mice (WT n = 55–56, APP/PS1 n = 31–34). (<b>F</b>) This effect was particularly pronounced at 4 and 8 months post-stroke, where APP/PS1 mice displayed greater cortical thickness than WT mice (WT n = 110–112, APP/PS1 n = 62–68). (<b>G</b>) Regarding hippocampal volume, stroke-operated mice exhibited lower right hippocampal volume compared to sham-operated mice (sham n = 45–46, stroke n = 43–45). (<b>H</b>) This stroke-induced reduction in hippocampal volume was also observed when considering the combined hippocampal volume at 0.5, 4, and 8 months post-surgery. Furthermore, the right hippocampal volume remained consistently smaller than the left hippocampal volume in stroke-operated mice. At 4 months after surgery, a similar interhemispheric difference was also measured among sham mice. (left sham n = 45–46, left stroke n = 43–45, right sham n = 45–46, right stroke n = 43–45). Data are presented as mean ± SEM. Significance is denoted as * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001.</p>
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<p>Longitudinal analysis of cerebral blood flow in male and female WT and APP/PS1 mice assessed at 0.5, 4, and 8 months post-stroke or -sham surgery. (<b>A</b>) Cortical CBF was consistently lower in the right hemisphere compared to the left at all time points (left n = 65–91, right n = 65–91). (<b>B</b>) Female mice had lower CBF in the right cortex compared to male animals during the post-surgery period (male n = 30–42, female n = 35–49). (<b>C</b>) At 0.5 and 8 months after stroke, female mice exhibited lower cortical CBF than males (male n = 60–84, female n = 70–98). (<b>D</b>) Four months after surgery, APP/PS1 mice had lower cortical CBF compared to their WT littermates (WT n = 90–114, APP/PS1 n = 40–68). (<b>E</b>) CBF in the hippocampus was significantly lower in the right hemisphere compared to the left at all time points (left n = 65–91, right n = 57–82). (<b>F</b>) At 4 months after surgery, stroke mice had higher CBF in the hippocampus than sham-operated animals (sham n = 70–92, stroke n = 52–86). (<b>G</b>) At 0.5 months after stroke induction, female animals showed lower CBF compared to males (male n = 56–81, female n = 66–93). (<b>H</b>) Male APP/PS1 mice had higher hippocampal CBF than male WT mice, whereas female APP/PS1 mice displayed lower CBF compared to male APP/PS1 mice (male WT n = 40–51, male APP/PS1 n = 16–30, female WT n = 42–56, female APP/PS1 n = 24–37). (<b>I</b>) In the right thalamus, stroke-operated mice displayed higher CBF over time compared to sham mice (sham n = 35–46, stroke n = 30–45). (<b>J</b>) Particularly at 4 and 8 months post-surgery, stroke-operated mice had higher thalamic CBF compared to sham-operated animals (sham n = 70–92, stroke n = 60–90). (<b>K</b>) Right thalamic CBF was lower than in the left hemisphere 0.5 months post-operation (left n = 65–91, right n = 65–91). (<b>L</b>) At 8 months after surgery, female mice showed lower CBF than male mice (male n = 65–91, female n = 65–91). (<b>M</b>) At 0.5 months post-stroke, female APP/PS1 mice showed lower CBF in the right thalamus than male APP/PS1 mice. At 4 months post-surgery, male APP/PS1 mice displayed lower CBF than male WT mice, and among APP/PS1 animals, females exhibited lower CBF than males. Additionally, an increase in CBF was observed in the right thalamus of male WT and female APP/PS1 mice between 0.5 and 4 months after surgery (male WT n = 22–27, male APP/PS1 n = 8–15, female WT n = 23–30, female APP/PS1 n = 12–19) (b: female WT vs. male WT, c: female APP/PS1 vs. male APP/PS1, d: male APP/PS1 vs. male WT). Data are presented as mean ± SEM. Significance is denoted as * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001.</p>
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<p>High-resolution voxel-wise CBF images at bregma level −1.94 in male and female WT and APP/PS1 mice, assessed at 0.5, 4, and 8 months post-stroke or -sham surgery.</p>
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<p>Longitudinal diffusion tensor imaging analysis. A comprehensive representation of fractional anisotropy (FA) and mean diffusivity (MD) in male and female WT and APP/PS1 mice assessed at 0.5, 4, and 8 months post-stroke or -sham surgery. (<b>A</b>) At 4 months post-stroke, the right cortex exhibited higher FA than the left cortex (left n = 67–76, right n = 67–76). (<b>B</b>) Comparing cortical FA at 4 months post-surgery, APP/PS1 sham mice showed lower FA than WT sham mice. In contrast, APP/PS1 stroke mice had higher cortical FA than APP/PS1 sham mice (WT sham n = 46–54, WT stroke n = 38–42, APP/PS1 sham n = 24–32, APP/PS1 stroke n = 22–32). (<b>C</b>) At 8 months post-stroke, APP/PS1 mice demonstrated lower cortical FA compared to WT mice (WT n = 88–94, APP/PS1 n = 46–64). (<b>D</b>) Additionally, at 8 months after surgery, female mice exhibited lower cortical FA than male mice (male n = 58–62, female n = 76–92). (<b>E</b>) Consistently, the right hippocampus showed lower FA than the left at all timepoints (left n = 67–76, right n = 67–76). (<b>F</b>) At both 4 and 8 months after stroke, the hippocampus of APP/PS1 mice displayed lower FA compared to that of WT mice (WT n = 88–94, APP/PS1 n = 46–64). (<b>G</b>) Across all time points, the right cortex consistently exhibited lower MD than the left cortex (left n = 67–76, right n = 67–76). (<b>H</b>) At 0.5 months post-stroke, the right hippocampus showed higher MD compared to the left hippocampus (left n = 67–76, right n = 67–76). (<b>I</b>) At the same time point, stroke mice displayed lower hippocampal MD than sham mice (sham n = 37–40, stroke n = 30–37). (<b>J</b>) Also, at 0.5 months after stroke, female WT mice demonstrated lower MD compared to male WT mice. Moreover, female APP/PS1 mice exhibited higher MD than their female WT counterparts (male WT n = 17–20, male APP/PS1 n = 10–13, female WT n = 25–27, female APP/PS1 n = 13–19). Data are presented as mean ± SEM. Significance is denoted as * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001.</p>
Full article ">Figure 7 Cont.
<p>Longitudinal diffusion tensor imaging analysis. A comprehensive representation of fractional anisotropy (FA) and mean diffusivity (MD) in male and female WT and APP/PS1 mice assessed at 0.5, 4, and 8 months post-stroke or -sham surgery. (<b>A</b>) At 4 months post-stroke, the right cortex exhibited higher FA than the left cortex (left n = 67–76, right n = 67–76). (<b>B</b>) Comparing cortical FA at 4 months post-surgery, APP/PS1 sham mice showed lower FA than WT sham mice. In contrast, APP/PS1 stroke mice had higher cortical FA than APP/PS1 sham mice (WT sham n = 46–54, WT stroke n = 38–42, APP/PS1 sham n = 24–32, APP/PS1 stroke n = 22–32). (<b>C</b>) At 8 months post-stroke, APP/PS1 mice demonstrated lower cortical FA compared to WT mice (WT n = 88–94, APP/PS1 n = 46–64). (<b>D</b>) Additionally, at 8 months after surgery, female mice exhibited lower cortical FA than male mice (male n = 58–62, female n = 76–92). (<b>E</b>) Consistently, the right hippocampus showed lower FA than the left at all timepoints (left n = 67–76, right n = 67–76). (<b>F</b>) At both 4 and 8 months after stroke, the hippocampus of APP/PS1 mice displayed lower FA compared to that of WT mice (WT n = 88–94, APP/PS1 n = 46–64). (<b>G</b>) Across all time points, the right cortex consistently exhibited lower MD than the left cortex (left n = 67–76, right n = 67–76). (<b>H</b>) At 0.5 months post-stroke, the right hippocampus showed higher MD compared to the left hippocampus (left n = 67–76, right n = 67–76). (<b>I</b>) At the same time point, stroke mice displayed lower hippocampal MD than sham mice (sham n = 37–40, stroke n = 30–37). (<b>J</b>) Also, at 0.5 months after stroke, female WT mice demonstrated lower MD compared to male WT mice. Moreover, female APP/PS1 mice exhibited higher MD than their female WT counterparts (male WT n = 17–20, male APP/PS1 n = 10–13, female WT n = 25–27, female APP/PS1 n = 13–19). Data are presented as mean ± SEM. Significance is denoted as * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001.</p>
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<p>Polarized light imaging (PLI) was conducted on post-mortem brains to evaluate myelin density and fiber orientation across different regions of interest, including the cortex, corpus callosum, hippocampus, and thalamus. This analysis utilized retardance maps for myelin density, as shown in the upper panel, and dispersion maps for fiber orientation, as shown in the lower panel. PLI was performed on male and female WT and APP/PS1 mice 8 months after sham and stroke surgeries when the mice were 12 months old. Retardance serves as an indicator of myelination, with lower values suggesting potential myelin degradation. Similarly, dispersion levels are a quantitative assessment of fiber orientation, where lower dispersion indicates better myelin quality. (<b>A</b>) In the cortex, notable genotype differences were observed in both male and female animals. Myelin density was higher in male APP/PS1 mice compared to male WT mice, whereas female APP/PS1 mice had lower myelin density compared to female WT mice. Moreover, female APP/PS1 mice had lower myelin density than their male counterparts (male WT n = 40, male APP/PS1 n = 26, female WT n = 44, female APP/PS1 n = 28). (<b>B</b>) In the hippocampus, myelin density was lower in APP/PS1 mice compared to WT mice (WT n = 83, APP/PS1 n = 56). (<b>C</b>) The same genotype difference was also present in the thalamus, where APP/PS1 mice displayed lower myelin density compared to WT mice (WT n = 92, APP/PS1 n = 58). (<b>D</b>) Additionally, in the thalamus, female mice had higher myelin density than male mice (male n = 68, female n = 82). (<b>F</b>) In terms of dispersion values measured in the cortex, female APP/PS1 mice exhibited lower values compared to both female WT mice and male APP/PS1 mice (male WT n = 40, male APP/PS1 n = 26, female WT n = 44, female APP/PS1 n = 28). (<b>G</b>) In the corpus callosum, dispersion values were generally lower among female mice than male mice (male n = 70, female n = 80). (<b>H</b>) Conversely, in the thalamus, female mice showed higher dispersion levels compared to their male counterparts (male n = 68, female n = 82). (<b>I</b>) In the thalamus, dispersion values were higher among stroke-operated mice in comparison to their sham-operated littermates (sham n = 80, stroke n = 70). Representative images of (<b>E</b>) retardance and (<b>J</b>) dispersion maps. Data are presented as mean ± SEM. Significance is denoted as * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001.</p>
Full article ">Figure 8 Cont.
<p>Polarized light imaging (PLI) was conducted on post-mortem brains to evaluate myelin density and fiber orientation across different regions of interest, including the cortex, corpus callosum, hippocampus, and thalamus. This analysis utilized retardance maps for myelin density, as shown in the upper panel, and dispersion maps for fiber orientation, as shown in the lower panel. PLI was performed on male and female WT and APP/PS1 mice 8 months after sham and stroke surgeries when the mice were 12 months old. Retardance serves as an indicator of myelination, with lower values suggesting potential myelin degradation. Similarly, dispersion levels are a quantitative assessment of fiber orientation, where lower dispersion indicates better myelin quality. (<b>A</b>) In the cortex, notable genotype differences were observed in both male and female animals. Myelin density was higher in male APP/PS1 mice compared to male WT mice, whereas female APP/PS1 mice had lower myelin density compared to female WT mice. Moreover, female APP/PS1 mice had lower myelin density than their male counterparts (male WT n = 40, male APP/PS1 n = 26, female WT n = 44, female APP/PS1 n = 28). (<b>B</b>) In the hippocampus, myelin density was lower in APP/PS1 mice compared to WT mice (WT n = 83, APP/PS1 n = 56). (<b>C</b>) The same genotype difference was also present in the thalamus, where APP/PS1 mice displayed lower myelin density compared to WT mice (WT n = 92, APP/PS1 n = 58). (<b>D</b>) Additionally, in the thalamus, female mice had higher myelin density than male mice (male n = 68, female n = 82). (<b>F</b>) In terms of dispersion values measured in the cortex, female APP/PS1 mice exhibited lower values compared to both female WT mice and male APP/PS1 mice (male WT n = 40, male APP/PS1 n = 26, female WT n = 44, female APP/PS1 n = 28). (<b>G</b>) In the corpus callosum, dispersion values were generally lower among female mice than male mice (male n = 70, female n = 80). (<b>H</b>) Conversely, in the thalamus, female mice showed higher dispersion levels compared to their male counterparts (male n = 68, female n = 82). (<b>I</b>) In the thalamus, dispersion values were higher among stroke-operated mice in comparison to their sham-operated littermates (sham n = 80, stroke n = 70). Representative images of (<b>E</b>) retardance and (<b>J</b>) dispersion maps. Data are presented as mean ± SEM. Significance is denoted as * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001.</p>
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<p>Immunohistochemical analysis of ionized calcium-binding adapter molecule 1 (IBA-1) as a measure of neuroinflammation. IBA-1+ microglia/macrophages were quantified across the cortex, hippocampus, and thalamus in male and female, sham- or stroke-operated WT and APP/PS1 mice, 8 months post-surgery at the age of 12 months. (<b>A</b>) Female WT mice displayed a lower amount of activated microglia in the cortex compared to male WT mice. Moreover, both female and male APP/PS1 mice exhibited a higher count of activated microglia in the cortex than their respective WT littermates (male WT n = 50, male APP/PS1 n = 22, female WT n = 56, female APP/PS1 n = 24). (<b>B</b>) In both cortical hemispheres, APP/PS1 mice had an increased amount of activated microglia compared to WT animals (left WT n = 53, left APP/PS1 n = 23, right WT n = 53, right APP/PS1 n = 23). (<b>C</b>) In the hippocampus, female mice displayed fewer activated microglia than male mice (male n = 73, female n = 80). (<b>D</b>) Notably, in the left hippocampus, female stroke mice displayed a lower amount of activated microglia compared to male stroke mice (male sham n = 18, male stroke n = 19, female sham n = 22, female stroke n = 18). (<b>E</b>) APP/PS1 mice also had a higher count of activated microglia in the combined hippocampus than WT mice (WT n = 105, APP/PS1 n = 48), (<b>F</b>) with this genotype effect being particularly detected in the left hippocampus (WT n = 53, APP/PS1 n = 24). (<b>G</b>) In the thalamus, stroke-operated APP/PS1 mice displayed a significantly lower count of activated microglia compared to both stroke-operated WT mice and sham-operated APP/PS1 mice (WT sham n = 52, WT stroke n = 52, APP/PS1 sham n = 16, APP/PS1 stroke n = 24). Representative images of IBA-1 staining in the left (L) and right (R) (<b>H</b>) cortex, (<b>I</b>) hippocampus, and (<b>J</b>) thalamus The boxes indicate regions that are visualized at higher magnification in the upper corners. (black scale bar = 200 µm; red scale bar: 100 µm). Data are presented as mean ± SEM. Significance is denoted as * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001.</p>
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<p>(<b>A</b>) Immunohistochemical analysis of amyloid beta (Aβ) in the hippocampus of male and female, sham- or stroke-operated APP/PS1 mice, 8 months post-surgery at the age of 12 months. The relative area covered with Aβ deposition was larger in female mice compared to male mice (male n = 30, female n = 36). (<b>B</b>) Representative images of WO-2 staining in the hippocampus (black scale bar = 200 µm). Data are presented as mean ± SEM. Significance is denoted as ** <span class="html-italic">p</span> &lt; 0.001.</p>
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20 pages, 572 KiB  
Article
Channel Estimation for Massive MIMO Systems via Polarized Self-Attention-Aided Channel Estimation Neural Network
by Shuo Yang, Yong Li, Lizhe Liu, Jing Xia, Bin Wang and Xingjian Li
Entropy 2025, 27(3), 220; https://doi.org/10.3390/e27030220 - 21 Feb 2025
Viewed by 228
Abstract
Research on deep learning (DL)-based channel estimation for massive multiple-input multiple-output (MIMO) communication systems has attracted considerable interest in recent years. In this paper, we propose a DL-assisted channel estimation algorithm that transforms the original channel estimation problem into an image denoising problem, [...] Read more.
Research on deep learning (DL)-based channel estimation for massive multiple-input multiple-output (MIMO) communication systems has attracted considerable interest in recent years. In this paper, we propose a DL-assisted channel estimation algorithm that transforms the original channel estimation problem into an image denoising problem, contrasting it with traditional experience-based channel estimation methods. We establish a new polarized self-attention-aided channel estimation neural network (PACE-Net) to achieve efficient channel estimation. This approach addresses the limitations of the conventional methods, particularly their low accuracy and high computational complexity. In addition, we construct a channel dataset to facilitate the training and testing of PACE-Net. The simulation results show that the proposed DL-assisted channel estimation algorithm has better normalization mean square error (NMSE) performance compared with the traditional algorithms and other DL-assisted algorithms. Furthermore, the computational complexity of the proposed DL-assisted algorithm is significantly lower than that of the traditional minimum mean square error (MMSE) channel estimation algorithm. Full article
(This article belongs to the Special Issue Wireless Communications: Signal Processing Perspectives)
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<p>Block diagram of massive MIMO communication system structure.</p>
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<p>Flowchart of a channel estimation algorithm utilizing a polarized self-attention-assisted neural network.</p>
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<p>PACE-Net model structure diagram.</p>
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<p>ReLU and LeakyReLU activation function curves.</p>
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<p>The polarized self-attention (PSA) block under the parallel layout.</p>
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<p>The polarized self-attention (PSA) block under the sequential layout.</p>
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<p>Loss function change curve of training set/validation set during model training process.</p>
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<p>Comparison of NMSE performance of different algorithms under independent channel.</p>
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<p>Effect of antenna correlation on the performance of each algorithm, SNR = 0 dB.</p>
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<p>Effect of antenna correlation on the performance of each algorithm, SNR = 5 dB.</p>
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<p>Effect of the number of antennas on the performance of the algorithm, <math display="inline"><semantics> <mrow> <mi mathvariant="italic">a</mi> <mo>=</mo> <mn>0.2</mn> </mrow> </semantics></math>.</p>
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<p>PSA module performance validation, <math display="inline"><semantics> <mrow> <mi mathvariant="italic">a</mi> <mo>=</mo> <mn>0.2</mn> </mrow> </semantics></math>.</p>
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<p>Performance validation of LeakyReLU activation function, <math display="inline"><semantics> <mrow> <mi mathvariant="italic">a</mi> <mo>=</mo> <mn>0.2</mn> </mrow> </semantics></math>.</p>
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<p>Verification of dropout performance, <math display="inline"><semantics> <mrow> <mi>a</mi> <mo>=</mo> <mn>0.2</mn> </mrow> </semantics></math>.</p>
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11 pages, 4311 KiB  
Article
Electrically Tunable Metasurface for Multi-Polarized Reflection
by Bing Li, Yunhan Wang, Chunan Wang and Shiqi Liu
Remote Sens. 2025, 17(4), 700; https://doi.org/10.3390/rs17040700 - 18 Feb 2025
Viewed by 241
Abstract
Most electromagnetic metasurfaces only control a single property of electromagnetic waves, such as the phase, amplitude, polarization or frequency, leading to a shortage in capacity and security in communication and a decrease in radar imaging efficiency. By switching the states of four PIN [...] Read more.
Most electromagnetic metasurfaces only control a single property of electromagnetic waves, such as the phase, amplitude, polarization or frequency, leading to a shortage in capacity and security in communication and a decrease in radar imaging efficiency. By switching the states of four PIN diodes soldered between adjacent resonant arms, cross-polarization and co-polarization reflected waves both with a 1-bit phase can be implemented. The simulation results demonstrate that the proposed metasurface operates within a frequency band of 5.7 GHz to 5.88 GHz, covering ISM 5.8 GHz. Within its operational frequency range, in the cross-polarization reflection case, the losses of the 1-bit phase reflected wave are from 1 dB to 1.5 dB, with a high polarization conversion rate exceeding 91% and even reaching 99%. For the co-polarization reflection case, the losses of the 1-bit reflected wave are from 0.3 dB to 2 dB, and the polarization conversion is almost 100%. The phase difference of the reflected wave in both cases can be realized as about 180°, which satisfies the 1-bit phase requirement for building a good property of beam steering. Upon constructing a 10 × 10 small array, the cross-polarized reflection beam can be steered within the range of elevation angle from 0° to 45° and the elevation angle from 0° to 30° in the co-polarized reflection case. Full article
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<p>Structure of the metasurface’s unit cell. p = 25 mm, h = 1.6 mm, l = 10.4 mm, w = 0.7 mm, d = 2 mm, R = 0.5 mm, and r = 0.3 mm.</p>
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<p>Reflection coefficient and phase difference in (<b>a</b>) CP1, (<b>b</b>) CP2, (<b>c</b>) CoP1 and (<b>d</b>) CoP2. Rectangle shadow is working band (5.7~5.88 GHz).</p>
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<p>Reflection loss in (<b>a</b>) CP1 and CP2 and (<b>b</b>) CoP1 and CoP2. (<b>c</b>) PCR in CP1, CP2, CoP1 and CoP2. Rectangle shadow is working band (5.7~5.88 GHz).</p>
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<p>Phase difference in CP case and CoP case. Rectangle shadow is working band (5.7~5.88 GHz).</p>
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<p>Surface current at 5.8 GHz. (<b>a</b>) CP1. (<b>b</b>) CP2. (<b>c</b>) CoP1. The dashed circular outlines indicate the current loop. (<b>d</b>) CoP2. The closed dashed lines indicate the self-resonant current path.</p>
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<p>The total reflected far-field at 5.8 GHz shaped in different angles. (<b>a</b>) CP case. (<b>b</b>) CoP case. Near the far-field is the metasurface’s configuration. “0” represents CP1 or CoP1, and “1” illustrates CP2 or CoP2.</p>
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<p>The total reflected far-field at 5.8 GHz shaped in different angles. (<b>a</b>) CP case. (<b>b</b>) CoP case. Near the far-field is the metasurface’s configuration. “0” represents CP1 or CoP1, and “1” illustrates CP2 or CoP2.</p>
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35 pages, 27811 KiB  
Article
Machine Learning to Retrieve Gap-Free Land Surface Temperature from Infrared Atmospheric Sounding Interferometer Observations
by Fabio Della Rocca, Pamela Pasquariello, Guido Masiello, Carmine Serio and Italia De Feis
Remote Sens. 2025, 17(4), 694; https://doi.org/10.3390/rs17040694 - 18 Feb 2025
Viewed by 288
Abstract
Retrieving LST from infrared spectral observations is challenging because it needs separation from emissivity in surface radiation emission, which is feasible only when the state of the surface–atmosphere system is known. Thanks to its high spectral resolution, the Infrared Atmospheric Sounding Interferometer (IASI) [...] Read more.
Retrieving LST from infrared spectral observations is challenging because it needs separation from emissivity in surface radiation emission, which is feasible only when the state of the surface–atmosphere system is known. Thanks to its high spectral resolution, the Infrared Atmospheric Sounding Interferometer (IASI) instrument onboard Metop polar-orbiting satellites is the only sensor that can simultaneously retrieve LST, the emissivity spectrum, and atmospheric composition. Still, it cannot penetrate thick cloud layers, making observations blind to surface emissions under cloudy conditions, with surface and atmospheric parameters being flagged as voids. The present paper aims to discuss a downscaling–fusion methodology to retrieve LST missing values on a spatial field retrieved from spatially scattered IASI observations to yield level 3, regularly gridded data, using as proxy data LST from the Spinning Enhanced Visible and Infrared Imager (SEVIRI) flying on Meteosat Second Generation (MSG) platform, a geostationary instrument, and from the Advanced Very High-Resolution Radiometer (AVHRR) onboard Metop polar-orbiting satellites. We address this problem by using machine learning techniques, i.e., Gradient Boosting, Random Forest, Gaussian Process Regression, Neural Network, and Stacked Regression. We applied the methodology over the Po Valley region, a very heterogeneous area that allows addressing the trained models’ robustness. Overall, the methods significantly enhanced spatial sampling, keeping errors in terms of Root Mean Square Error (RMSE) and bias (Mean Absolute Error, MAE) very low. Although we demonstrate and assess the results primarily using IASI data, the paper is also intended for applications to the IASI follow-on, that is, IASI Next Generation (IASI-NG), and much more to the Infrared Sounder (IRS), which is planned to fly this year, 2025, on the Meteosat Third Generation platform (MTG). Full article
(This article belongs to the Special Issue Remote Sensing in Geomatics (Second Edition))
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<p>The red box indicates the Po Valley target region with the CLC 2018 as shapefile.</p>
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<p>Flowchart of the proposed framework. (<b>a</b>) Retrieval of LST; (<b>b</b>) Training; (<b>c</b>) L3 LST.</p>
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<p>Comparison of L2 IASI observations and the derived prediction mask for August 2022. The left panel shows the spatial distribution of L2 observations across the 9 years of data, while the right panel shows the spatial domain used for prediction.</p>
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<p>Comparison of MAE cross validated errors of the tested ML algorithms: Random Forest (blue), Boosting (orange), Neural Network (yellow), Gaussian Process Regression (purple), and Stacked Regression (green). The numbers in the legend represent the average MAE for all methods calculated across all months.</p>
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<p>Comparison of RMSE cross validated errors of the tested ML algorithms: Random Forest (blue), Boosting (orange), Neural Network (yellow), Gaussian Process Regression (purple), and Stacked Regression (green). The numbers in the legend represent the average RMSE for all methods calculated across all months.</p>
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<p>Example of the L3 LST for the months January–June. The first column represents the IASI LST L2 observations, while the second column shows the LST L3 predicted with Stacked Regression; each row represents a month.</p>
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<p>Example of the L3 LST for the months July–December. The first column represents the IASI LST L2 observations, while the second column shows the LST L3 predicted with Stacked Regression; each row represents a month.</p>
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<p>Comparison between the predicted LST for August 2022 and the mean of nine years of AVHRR and SEVIRI data for the same month. The top-left panel shows the IASI L2 observations, while the right panel displays the difference maps with SEVIRI (<b>top</b>) and AVHRR (<b>bottom</b>) including also the L2 observations represented by the small black dots. The bottom-left panel presents the KDE plot of these differences, including the mean and standard deviation of the errors.</p>
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<p>Comparison between the predicted LST for March 2022 and the mean of nine years of AVHRR and SEVIRI data for the same month. The top-left panel shows the IASI L2 observations, while the right panel displays the difference maps with SEVIRI (<b>top</b>) and AVHRR (<b>bottom</b>) including also the L2 observations represented by the small black dots. The bottom-left panel presents the KDE plot of these differences, including the mean and standard deviation of the errors.</p>
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<p>L2/L3 Differences IASI - MODIS for the months January–June. The first column displays the L2/L3 differences using KDE plots with the mean and standard deviation: the red curves display the L2 errors, and the blue curves display the L3 errors. The second column shows the scatterplots between the predicted IASI L3 LST values and the MODIS L3 LST values, the linear fits, and the <math display="inline"><semantics> <mrow> <msup> <mi mathvariant="normal">R</mi> <mn>2</mn> </msup> </mrow> </semantics></math> indexes.</p>
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<p>L2/L3 Differences IASI - MODIS for the months July–December. The first column displays the L2/L3 differences using KDE plots with the mean and standard deviation: the red curves display the L2 errors, and the blue curves display the L3 errors. The second column shows the scatterplots between the predicted IASI L3 LST values and the MODIS L3 LST values, the linear fits, and the <math display="inline"><semantics> <mrow> <msup> <mi mathvariant="normal">R</mi> <mn>2</mn> </msup> </mrow> </semantics></math> indexes.</p>
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<p>Comparison LSTs from IASI, AVHRR, SEVIRI and MODIS for the months January–June. The first column displays the L2 differences using KDE plots with the mean and standard deviation: the blue curves represent the L2 differences between IASI and AVHRR, the red curves represent the L2 differences between IASI and SEVIRI, and the yellow curves represent the L2 differences between IASI and MODIS. The second column shows the same differences using boxplots, with the <math display="inline"><semantics> <mrow> <msup> <mi mathvariant="normal">R</mi> <mn>2</mn> </msup> </mrow> </semantics></math> indexes included on the <span class="html-italic">x</span>-axis.</p>
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<p>Comparison LSTs from IASI, AVHRR, SEVIRI and MODIS for the months July–December. The first column displays the L2 differences using KDE plots with the mean and standard deviation: the blue curves represent the L2 differences between IASI and AVHRR, the red curves represent the L2 differences between IASI and SEVIRI, and the yellow curves represent the L2 differences between IASI and MODIS. The second column shows the same differences using boxplots, with the <math display="inline"><semantics> <mrow> <msup> <mi mathvariant="normal">R</mi> <mn>2</mn> </msup> </mrow> </semantics></math> indexes included on the <span class="html-italic">x</span>-axis.</p>
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17 pages, 24593 KiB  
Article
Enhanced PolSAR Image Segmentation with Polarization Channel Fusion and Diffusion-Based Probability Modeling
by Hao Chen, Yuzhuo Hou, Xiaoxiao Fang and Chu He
Electronics 2025, 14(4), 791; https://doi.org/10.3390/electronics14040791 - 18 Feb 2025
Viewed by 213
Abstract
With the advancement of polarimetric synthetic aperture radar (PolSAR) imaging technology and the growing demand for image interpretation, extracting meaningful land cover information from PolSAR images has become a key research focus. To address the segmentation challenge, we propose an innovative method. First, [...] Read more.
With the advancement of polarimetric synthetic aperture radar (PolSAR) imaging technology and the growing demand for image interpretation, extracting meaningful land cover information from PolSAR images has become a key research focus. To address the segmentation challenge, we propose an innovative method. First, features from co-polarization and cross-polarization channels are separately used as dual inputs, and a cross-attention mechanism effectively fuses these features to capture correlations between different polarization information. Second, a diffusion framework is employed to jointly model target features and class probabilities, aiming to improve segmentation accuracy by learning and fitting the probabilistic distribution of target labels. Finally, experimental results demonstrate that the proposed method achieves superior performance in PolSAR image segmentation, effectively managing complex polarization relationships while offering robustness and broad application potential. Full article
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<p>PolSAR image segmentation framework.</p>
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<p>Conditional diffusion for image segmentation.</p>
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<p>Hybrid modeling framework for PolSAR segmentation.</p>
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<p>Dual-path polarization channel feature fusion module by cross attention (DCFM).</p>
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<p>The used PolSAR datasets.</p>
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<p>Segmentation rResults of the SanALOS2 dataset. (<b>a</b>) Ground truth. (<b>b</b>) FCNs. (<b>c</b>) PSPNet. (<b>d</b>) EmaNet. (<b>e</b>) DANet. (<b>f</b>) SETR. (<b>g</b>) Segformer. (<b>h</b>) Proposal.</p>
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<p>Segmentation results of the HainanC dataset. (<b>a</b>) Ground truth. (<b>b</b>) FCNs. (<b>c</b>) PSPNet. (<b>d</b>) EmaNet. (<b>e</b>) DANet. (<b>f</b>) SETR. (<b>g</b>) Segformer. (<b>h</b>) Proposal.</p>
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<p>OA curves of different methods in the training process on the Hainan dataset.</p>
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