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17 pages, 3314 KiB  
Article
Vitellogenins Level as a Biomarker of the Honeybee Colony Strength in Urban and Rural Conditions
by Łukasz Nicewicz, Agata Wanda Nicewicz and Mirosław Nakonieczny
Insects 2025, 16(1), 25; https://doi.org/10.3390/insects16010025 (registering DOI) - 29 Dec 2024
Abstract
The study aimed to verify whether urban beekeeping affects the strength of the honeybee (Apis mellifera) colonies from urban apiaries and the variability of the crucial for their health and long-life protein—vitellogenins. For this purpose, honeybees were kept in two locations—in [...] Read more.
The study aimed to verify whether urban beekeeping affects the strength of the honeybee (Apis mellifera) colonies from urban apiaries and the variability of the crucial for their health and long-life protein—vitellogenins. For this purpose, honeybees were kept in two locations—in a city apiary on a roof in the city center and in agricultural areas. Each of the apiaries consisted of six colonies, with the sister queens artificially inseminated with semen from the same pool of drones. The bee colony strength and the variability of the vitellogenins in various tissues in foragers from both apiaries were analyzed from May to August. Here, we revealed that colonies from the urban apiary were more abundant than those from the rural apiary. We observed the compensation mechanism during periods of worker deficiency in the bee colony, which was expressed as a change in the Vgs level in the forager tissues. Using the vitellogenin level as a biomarker of the honeybee colony strength can predict the fate of colonies, especially those with low numbers. The high level of Vgs can be a candidate for bee colony depopulation biomarker. Full article
(This article belongs to the Section Insect Physiology, Reproduction and Development)
20 pages, 3102 KiB  
Article
Effect of Flowering Shading on Grain Yield and Quality of Durum Wheat in a Mediterranean Environment
by Giancarlo Pagnani, Alfredo Lorenzo, Nausicaa Occhipinti, Lisa Antonucci, Sara D’Egidio, Fabio Stagnari and Michele Pisante
Plants 2025, 14(1), 76; https://doi.org/10.3390/plants14010076 (registering DOI) - 29 Dec 2024
Abstract
The phenomenon known as “dimming” or shading, caused by the increase in aerosols, air pollutants, and population density, is reducing global radiation, including both direct solar radiation and radiation scattered by the atmosphere. This phenomenon poses a significant challenge for agricultural production in [...] Read more.
The phenomenon known as “dimming” or shading, caused by the increase in aerosols, air pollutants, and population density, is reducing global radiation, including both direct solar radiation and radiation scattered by the atmosphere. This phenomenon poses a significant challenge for agricultural production in many regions worldwide, with a global radiation decrease estimated between 1.4% and 2.7% per decade in areas between 25° N and 45° N. In particular, in Mediterranean regions, the production of durum wheat (Triticum turgidum L. subsp. Durum) is increasingly constrained by abiotic factors, such as spring/summer heat stress and drought, as well as reductions in solar radiation. Field experiments were conducted in Mosciano Sant’Angelo, Italy, over two cropping seasons (2016–2017 and 2017–2018) to evaluate the effects of photosynthetically active radiation (PAR) availability and nitrogen (N) fertilization on durum wheat. A split-plot design was used with two PAR levels (100% and 20% PAR) and three N rates (0, 100, and 250 kg ha−1). Results highlighted that full sunlight (NoSh) significantly increased grain yield (+25%), thousand kernel weight (+46%), and total gluten fractions (+16%) compared to shaded conditions (Sh). Chlorophyll content and NDVI values were highest under Sh combined with 250 kg N ha−1. Rainfall patterns strongly influenced productivity, with better vegetative growth in 2016–2017 and improved grain filling in 2017–2018. Nitrogen application significantly enhanced grain protein content, particularly under arid conditions. These findings emphasize the interaction between light availability and nitrogen management, suggesting that optimizing these factors can improve yield and quality in durum wheat under Mediterranean conditions. Full article
(This article belongs to the Section Plant–Soil Interactions)
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<p>Monthly rainfall (represented by bars) and average monthly temperatures (indicated by dots) were recorded throughout the entire durum wheat crop cycle during the 2016–2017 and 2017–2018 growing seasons in the experimental area.</p>
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<p>Thousand kernel weight (TKW, g) as recorded for durum wheat at harvest in the 2016–2017 and 2017–2018 cropping seasons. NoSh: 100% PAR; Sh: 20% PAR; 0_N: 0 Kg N ha<sup>−1</sup>; 100_N: 100 Kg N ha<sup>−1</sup>; 250_N: 250 Kg N ha<sup>−1</sup>. Data are averages ± standard errors of n = 3 independent replicates. Different lower case letters indicate significant differences at <span class="html-italic">p</span> &lt; 0.05 (Fisher’s LSD test).</p>
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<p>Grain yield (t ha<sup>−1</sup>) as recorded for durum wheat at harvest in the 2016–2017 and 2017–2018 cropping seasons. NoSh: 100% PAR; Sh: 20% PAR; 0_N: 0 Kg N ha<sup>−1</sup>; 100_N: 100 Kg N ha<sup>−1</sup>; 250_N: 250 Kg N ha<sup>−1</sup>. Data are averages ± standard errors of n = 3 independent replicates. Different lower case letters indicate significant differences at <span class="html-italic">p</span> &lt; 0.05 (Fisher’s LSD test).</p>
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<p>Grain protein content (GPC, %) as recorded for durum wheat at harvest in the 2016–2017 and 2017–2018 cropping seasons. NoSh: 100% PAR; Sh: 20% PAR; 0_N: 0 Kg N ha<sup>−1</sup>; 100_N: 100 Kg N ha<sup>−1</sup>; 250_N: 250 Kg N ha<sup>−1</sup>. Data are averages ± standard errors of n = 3 independent replicates. Different lower case letters indicate significant differences at <span class="html-italic">p</span> &lt; 0.05 (Fisher’s LSD test).</p>
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<p>Search content (%) as recorded for durum wheat at harvest in the 2016–2017 and 2017–2018 cropping seasons. NoSh: 100% PAR; Sh: 20% PAR; 0_N: 0 Kg N ha<sup>−1</sup>; 100_N: 100 Kg N ha<sup>−1</sup>; 250_N: 250 Kg N ha<sup>−1</sup>. Data are averages ± standard errors of n = 3 independent replicates. Different lower case letters indicate significant differences at <span class="html-italic">p</span> &lt; 0.05 (Fisher’s LSD test).</p>
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11 pages, 234 KiB  
Article
Response of Non-Irrigated Peanut to Multiple Rate Delayed Flumioxazin Applications
by Nicholas L. Hurdle, Timothy L. Grey, Juliana de Souza Rodrigues and W. Scott Monfort
Agronomy 2025, 15(1), 64; https://doi.org/10.3390/agronomy15010064 (registering DOI) - 29 Dec 2024
Abstract
Flumioxazin is crucial for peanut weed management across the United States with over 75% of growers applying it to control troublesome weed species. For maximum peanut yield, it is essential that weed control is maintained during weeks three through eight after planting. Peanut [...] Read more.
Flumioxazin is crucial for peanut weed management across the United States with over 75% of growers applying it to control troublesome weed species. For maximum peanut yield, it is essential that weed control is maintained during weeks three through eight after planting. Peanut injury due to flumioxazin PRE applied has been noted under unfavorable moisture or weather conditions, but also due to delays in application as growers plant hundreds of hectares on their farms. Research in Georgia (GA) investigated the response of non-irrigated peanut to flumioxazin PRE applied from 0 to 107 g ai/ha at 0 to 14 d after planting for cultivar Georgia-16HO. Trends during the 2020 through 2022 growing seasons indicated that as rate and time after planting of application increased, injury increased. Over 50% injury was noted in Tift County and 24% in Sumter County during the 2021 growing season. Peanut pod yield decreased while flumioxazin rate increased and time of application after planting was delayed in Tift County, but no differences were noted in Sumter County, potentially due to soil adsorption of the herbicide. Yield differences of up to 800 kg/ha were noted when comparing no herbicide being applied to the full application rate. The recorded injury coincided with large amounts of rainfall at both locations. It was also noted that peanut may be most sensitive to flumioxazin application injury between days seven and ten after planting. Full article
(This article belongs to the Special Issue Pest Control Technologies Applied in Peanut Production Systems)
24 pages, 6206 KiB  
Article
Fuel Load Models for Different Tree Vegetation Types in Sichuan Province Based on Machine Learning
by Hongrong Wang, Haoquan Chen, Hanmin Sheng, Kai Chen, Chen Dong and Zhiqiang Min
Forests 2025, 16(1), 42; https://doi.org/10.3390/f16010042 (registering DOI) - 29 Dec 2024
Abstract
(1) Objective: To improve forest fire prevention, this study provides a reference for forest fire risk assessment in Sichuan Province. (2) Methods: This research focuses on various forest vegetation types in Sichuan Province. Given data from 6848 sample plots, five machine learning models—random [...] Read more.
(1) Objective: To improve forest fire prevention, this study provides a reference for forest fire risk assessment in Sichuan Province. (2) Methods: This research focuses on various forest vegetation types in Sichuan Province. Given data from 6848 sample plots, five machine learning models—random forest, extreme gradient boosting (XGBoost), k-nearest neighbors, support vector machine, and stacking ensemble (Stacking)—were employed. Bayesian optimization was utilized for hyperparameter tuning, resulting in machine learning models for predicting forest fuel loads (FLs) across five different vegetation types. (3) Results: The FL model incorporates not only vegetation characteristics but also site conditions and climate data. Feature importance analysis indicated that structural factors (e.g., canopy closure, diameter at breast height, and tree height) dominated in cold broadleaf, subtropical broadleaf, and subtropical mixed forests, while climate factors (e.g., mean annual temperature and temperature seasonality) were more influential in cold coniferous and subtropical coniferous forests. Machine learning-based FL models outperform the multiple stepwise regression model in both fitting ability and prediction accuracy. The XGBoost model performed best for cold coniferous, cold broadleaf, subtropical broadleaf, and subtropical mixed forests, with coefficient of determination (R2) values of 0.79, 0.85, 0.81, and 0.83, respectively. The Stacking model excelled in subtropical coniferous forests, achieving an R2 value of 0.82. (4) Conclusions: This study establishes a theoretical foundation for predicting forest fuel capacity in Sichuan Province. It is recommended that the XGBoost model be applied to predict fuel loads (FLs) in cold coniferous forests, cold broadleaf forests, subtropical broadleaf forests, and subtropical mixed forests, while the Stacking model is suggested for predicting FLs in subtropical coniferous forests. Furthermore, this research offers theoretical support for forest fuel management, forest fire risk assessment, and forest fire prevention and control in Sichuan Province. Full article
(This article belongs to the Section Forest Inventory, Modeling and Remote Sensing)
14 pages, 4006 KiB  
Article
Characterization of a Natural Accession of Elymus sibiricus with In Situ Hybridization and Agronomic Evaluation
by Yizhuo Liu, Jiarui Ding, Chunfei Wu, Weiwei Song, Xinyu Zhao, Haibin Zhao, Yunfeng Qu, Hui Jin, Rui Zhang, Mingyao Li, Xinyu Yan, Liangyu Zhu, Yaqi Bao, Dianhao Liu, Xinling Li, Lei Cui, Hongjie Li and Yanming Zhang
Plants 2025, 14(1), 75; https://doi.org/10.3390/plants14010075 (registering DOI) - 29 Dec 2024
Abstract
Elymus sibiricus, valued for its perennial nature, broad adaptability, strong cold tolerance, and high economic value in forage production, plays a crucial role in combating grassland degradation, desertification, and salinization. Using morphological and cytogenetic methods, this study evaluated the cold tolerance, post-harvest [...] Read more.
Elymus sibiricus, valued for its perennial nature, broad adaptability, strong cold tolerance, and high economic value in forage production, plays a crucial role in combating grassland degradation, desertification, and salinization. Using morphological and cytogenetic methods, this study evaluated the cold tolerance, post-harvest regeneration capacity, and perennial characteristics of the E. sibiricus accession 20HSC-Z9 in the Harbin region of China from 2020 to 2023. This accession exhibited a germination rate of over 90% and a 100% green-up rate, with purple coleoptiles indicating its strong cold tolerance. Over the three growing seasons, 20HSC-Z9 maintained stable green-up and regeneration rates, confirming its perennial nature. Morphologically, 20HSC-Z9 had an average tiller count ranging from 56 to 74, similar to that of the control accession 20HSC-ES, and its plant height was significantly lower than that of 20HSC-IWG. Furthermore, 20HSC-Z9 produced over 100 grains per spike, with a seed setting rate exceeding 90%, and a thousand-grain weight comparable to that of 20HSC-IWG. The grain protein content of 20HSC-Z9 reached a maximum of 21.19%, greater than that of the control accessions (15.6% and 18.5%). Chromosome composition analysis, using sequential multicolor genomic in situ hybridization and multicolor fluorescence in situ hybridization, confirmed the StStHH genomic constitution of 20HSC-Z9 and revealed translocations between the St and H subgenome chromosomes. These results suggest that 20HSC-Z9 has significant potential as a new perennial forage grass germplasm for cold regions, suitable for further domestication and breeding efforts. Full article
(This article belongs to the Section Plant Genetics, Genomics and Biotechnology)
15 pages, 3201 KiB  
Article
Fish Larval Assemblage Associated with an Eastern Tropical Pacific Coral Reef: Seasonal and Interannual Variability
by Juan José Gallego-Zerrato, Diego Fernando Córdoba-Rojas and Alan Giraldo
Diversity 2025, 17(1), 23; https://doi.org/10.3390/d17010023 (registering DOI) - 29 Dec 2024
Abstract
The seasonal and interannual temporal variation in the composition, richness, diversity, and similarity of fish larval assemblages associated with an Eastern Tropical Pacific (ETP) coral reef system was studied in March (cold water) and September (warm water) during the years 2017, 2018, and [...] Read more.
The seasonal and interannual temporal variation in the composition, richness, diversity, and similarity of fish larval assemblages associated with an Eastern Tropical Pacific (ETP) coral reef system was studied in March (cold water) and September (warm water) during the years 2017, 2018, and 2019. Throughout the study period, we collected 4779 fish larvae and identified 88 taxa, encompassing 46 families. This increased the total number of recorded fish taxa for the region to 146. Fish larvae were collected by daytime and nighttime surface trawls, using a bongo net 30 cm in diameter and 180 cm in length, equipped with mesh sizes of 300 and 500 μm. The species diversity and abundance of ichthyoplankton over this ETP coral reef changed by intra-annual variation of the hydrological conditions of the upper layer of the sea. Six significant assemblages were identified (SIMPROF, p < 0.05), each one associated with each sampling period (ANOSIM, R = 0.764); Cetengraulis mysticetus, Diaphus pacificus, Anchoa sp., Anisotremus sp., Bremaceros bathymaster, Oligoplites saurus, Caranx sp., Seriola sp., Gobiidae sp., Microgobius sp., and Synodus evermanni were the species that contributed to dissimilitude between groups. Canonical correspondence analysis revealed significant associations between specific larval fish taxa abundance and temperature, salinity, dissolved oxygen, and zooplankton biomass. Overall, the assemblage of ichthyoplankton in this ETP coral reef system is sensitive to seasonal changes in water column hydrographic conditions. Full article
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<p>Geographical location of Gorgona Island and sampling stations on the La Azufrada reef. The area of the La Azufrada reef plain and slope, the position of La Azufrada on Gorgona Island, and the position of Gorgona Island in the Eastern Tropical Pacific are highlighted.</p>
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<p>Zooplankton biomass and fish larval density present in the water column associated with the La Azufrada coral reef during March and September 2017 to 2019. The y-axis is in logarithmic scale.</p>
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<p>Spearman correlation showing the relationship between zooplankton biomass and larval density in the study area. The two axes are in logarithmic scale.</p>
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<p>Similarity dendrogram of the fish larval assemblage present in the water column associated with La Azufrada reef in the March-September periods between 2017 and 2019. The first term is the station number (1 to 9) and sampling month (M: March, S: September). The last term is the year (2017, 2018, or 2019).</p>
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<p>Canonical correspondence analysis using the abundance of species found with frequency &gt;5%. The first two canonical axes explain 67% of the total variance. DO: dissolved oxygen, S: salinity, T: temperature, Chl-a: chlorophyll-a.</p>
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20 pages, 5685 KiB  
Article
Identification of Aerosols’ Optical Properties in the Caribbean Area Using Ascending Hierarchical Clustering Analysis
by Lovely Euphrasie-Clotilde, Thomas Plocoste, France-Nor Brute, Cristian Velasco-Merino, Davis Mateos and Carlos Toledano
Sustainability 2025, 17(1), 177; https://doi.org/10.3390/su17010177 (registering DOI) - 29 Dec 2024
Viewed by 2
Abstract
Atmospheric aerosols significantly impact air quality, human health, and regional climate, with regions like the Caribbean Basin affected by various aerosol types, including marine, anthropogenic, and desert dust particles. This study utilizes Agglomerative Hierarchical Clustering (AHC) to analyze more than a decade of [...] Read more.
Atmospheric aerosols significantly impact air quality, human health, and regional climate, with regions like the Caribbean Basin affected by various aerosol types, including marine, anthropogenic, and desert dust particles. This study utilizes Agglomerative Hierarchical Clustering (AHC) to analyze more than a decade of Aerosol Robotic Network (AERONET) data (2007–2023) from four Caribbean islands: Barbados, Guadeloupe, Puerto Rico, and Cuba. We examined sixteen physical parameters, including Aerosol Optical Depth (AOD), Angstrom Exponent (AE), and Volume Particle Size Distribution (VPSD), to identify distinct aerosol regimes and groups of daily measurements displaying similar aerosol optical properties. The originality of this work lies in the significant number of parameters considered to achieve a classification free of arbitrary orientation. The clustering method identified specific periods and aerosol characteristics, revealing seasonal patterns of background marine aerosols and Saharan dust events. By referring to existing research and using analysis tools such as VPSD and AE versus AOD representation, we aimed to define value ranges of physical parameters attributable to marine, dust, and mixed aerosols in the Caribbean region. The results underscore the diversity of aerosol sources and their seasonal variations across the Caribbean, providing critical insights for improving regional air quality management. This classification approach integrates comprehensive aerosol properties and is reinforced by the analysis of atmospheric circulation using the HYSPLIT model. These findings not only advance the characterization of aerosol regimes but also contribute to sustainable air quality management practices by providing actionable data to mitigate the adverse health and environmental impacts of aerosols. Full article
(This article belongs to the Section Air, Climate Change and Sustainability)
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<p>Map of the Caribbean area with, respectively, from south to north, Barbados (BAR, purple triangle), Guadeloupe (GPE, red dot), Puerto Rico (PR, orange square), and Cuba (CU, yellow diamond).</p>
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<p>Dendrogram of AERONET data for BAR, GPE, PR, and CU from 2007 to 2023. Colors simply distinguish groups of classification. BAR and GPE both present two classes while PR and CU cases display three classes. For Barbados, N (Class 1) = 864, and N (Class 2) = 1097; for Guadeloupe, N (class 1) = 314, N (class 2) = 692, and N (class 3) = 133; for Puerto Rico, N (class 1) = 1055, and N (class 2) = 370; for Cuba, N (class 1) = 1053, and N (class 2) = 1080.</p>
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<p>Dendrogram of AERONET data for BAR, GPE, PR, and CU from 2007 to 2023. Colors simply distinguish groups of classification. BAR and GPE both present two classes while PR and CU cases display three classes. For Barbados, N (Class 1) = 864, and N (Class 2) = 1097; for Guadeloupe, N (class 1) = 314, N (class 2) = 692, and N (class 3) = 133; for Puerto Rico, N (class 1) = 1055, and N (class 2) = 370; for Cuba, N (class 1) = 1053, and N (class 2) = 1080.</p>
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<p>Dendrogram of AERONET data for BAR, GPE, PR, and CU from 2007 to 2023. Colors simply distinguish groups of classification. BAR and GPE both present two classes while PR and CU cases display three classes. For Barbados, N (Class 1) = 864, and N (Class 2) = 1097; for Guadeloupe, N (class 1) = 314, N (class 2) = 692, and N (class 3) = 133; for Puerto Rico, N (class 1) = 1055, and N (class 2) = 370; for Cuba, N (class 1) = 1053, and N (class 2) = 1080.</p>
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<p>Seasonal distribution of monthly frequency related to the AHC for (<b>a</b>) the background atmosphere and (<b>b</b>) the dusty atmosphere.</p>
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<p>Distribution of AE depends on AOD data (daily mean from 2007 to 2023).</p>
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<p>VPSD average related to classes for GPE, PR, BAR, and CU; (<b>a</b>) groups of the classes identified as dust cases; (<b>b</b>) groups of the VPSD of the background atmosphere classes.</p>
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<p>VPSD average related to classes for GPE, PR, BAR, and CU; (<b>a</b>) groups of the classes identified as dust cases; (<b>b</b>) groups of the VPSD of the background atmosphere classes.</p>
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<p>Representation of the different types of atmospheric circulation, along with the percentages of cases that meet the criteria for marine aerosols/the background, as mentioned above. The back-trajectories illustrate the case for Guadeloupe (GPE). It is noteworthy that no significant differences were observed among the various islands studied.</p>
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<p>Representation of the different types of atmospheric circulation, along with the percentages of cases that meet the criteria for pure dust aerosols as mentioned above. The back-trajectories illustrate the case for Guadeloupe (GPE). It is noteworthy that no significant differences were observed among the various islands studied.</p>
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17 pages, 4208 KiB  
Article
Assessing Thermal Comfort and Indoor Air Quality: In an Educational Facility of a Semi-Arid Climate Zone
by Kemalettin Parmaksiz, Mehmet Irfan Yesilnacar and Abdullah Izzeddin Karabulut
Atmosphere 2025, 16(1), 29; https://doi.org/10.3390/atmos16010029 (registering DOI) - 29 Dec 2024
Viewed by 84
Abstract
There are three main approaches to human thermal comfort; a psychological approach, a thermo-physiological approach, and an approach based on human energy balance. According to the ISO 7730 and ASHRAE Standard 55-2023 standards, the psychological approach defines thermal comfort as a mental state [...] Read more.
There are three main approaches to human thermal comfort; a psychological approach, a thermo-physiological approach, and an approach based on human energy balance. According to the ISO 7730 and ASHRAE Standard 55-2023 standards, the psychological approach defines thermal comfort as a mental state in which individuals feel satisfied with their surrounding environment. According to this definition, thermal comfort is very subjective and may vary between individuals, as well as according to the environment and climate. This study aimed to evaluate the thermal comfort levels of students in primary and high school classrooms situated within the semi-arid climatic conditions of Şanlıurfa. For this purpose, 15 Temmuz Şehitleri Secondary School, Kadir Evliyaoğlu College, and TOBB Science High School in Şanlıurfa were chosen as fieldwork locations. Within the scope of the study, the climatic conditions (classroom temperature, air velocity, humidity, radiant temperature, Tw, Tg carbon dioxide) were measured, and how the students felt under the thermal conditions of these classrooms was evaluated. The study encompasses both the heating season (winter) and the non-heating season (summer). Based on the findings obtained from the study, PMV (Predicted Mean Vote) and PPD (Predicted Percentage Dissatisfied) values and whether they are suitable thermal comfort for the people in these places tried to be determined by mathematical modeling and standards such as ASHRAE Standard 55-2023. While PMV values ranged between −0.58 (North) and 2.53 (East+South+West), PPD values were observed between 5% (South and some North facades) and 94% (East+South+West). While the South facade offers values close to the comfort range of 0.01–0.02 in terms of PMV, the East+South+West facade shows serious thermal discomfort with a PMV value of 2.53 and a PPD value of 94%. Full article
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<p>15 Temmuz Şehitleri School Floor plan.</p>
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<p>TOBB Science High School Floor Plan.</p>
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<p>Kadir Evliyaoğlu College floor plan.</p>
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<p>Placement of measurement equipment.</p>
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<p>CBE Thermal Comfort Tool home page.</p>
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<p>PMV and PPD values of the classes during the summer period.</p>
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<p>PMV and PPD values of classes during winter.</p>
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<p>CO<sub>2</sub> ppm values measured in classrooms during summer.</p>
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<p>CO<sub>2</sub> ppm values measured in classrooms during winter.</p>
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<p>Grade point average data for the classes in which the students are located according to fronts.</p>
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18 pages, 2186 KiB  
Article
Zooplankton as Indicator of Ecological Status in the Streževo Reservoir (North Macedonia)
by Tea Tomljanović, Orhideja Tasevska, Maria Špoljar, Goce Kostoski, Ines Radanović, Elizabeta Veljanoska Sarafiloska, Suzana Patčeva, Jovica Lešoski, Spase Shumka and Tvrtko Dražina
Sustainability 2025, 17(1), 171; https://doi.org/10.3390/su17010171 (registering DOI) - 29 Dec 2024
Viewed by 106
Abstract
This study examined the ecological status of the Streževo Reservoir in North Macedonia, focusing on zooplankton as an indicator of water quality. Built in 1982, the Streževo Reservoir serves several purposes, including irrigation, water supply, and hydropower generation. The research project investigated the [...] Read more.
This study examined the ecological status of the Streževo Reservoir in North Macedonia, focusing on zooplankton as an indicator of water quality. Built in 1982, the Streževo Reservoir serves several purposes, including irrigation, water supply, and hydropower generation. The research project investigated the seasonal and vertical variation in zooplankton abundance and biomass as well as the influence of environmental factors. Sampling was conducted seasonally (spring, summer, and autumn) in 2010 and 2011 across the longitudinal profile (epilimnion, metalimnion, and hypolimnion) of the reservoir at three sampling stations: the inflow of the Šemnica River, a central station in open water, and a site near the dam. The Streževo Reservoir is characterized by significantly pronounced seasonal and vertical temperature stratifications. The species diversity of the zooplankton was low, with only 21 taxa identified. Seasonal oscillations in abundance were statistically significant, with maximum values in the summer period and minimum values in spring. The Shannon diversity index displayed the lowest diversity values in the autumn, in the hypolimnion, and the highest values in the summer, in the metalimnion. The RDA analysis showed that temperature was the most important predictor of zooplankton abundance distribution, followed by Chl a concentration and TN. According to the Zooplankton Index of Quality Assessment (Zoo-IQ), during the investigated period the reservoir had good water quality in all three studied seasons, as well as through the whole profile. Overall, the study highlights the importance of zooplankton as an indicator of water quality and provides valuable insights into the ecological status of the Streževo Reservoir. The novelty of this study lies in its comprehensive examination of the interconnected dynamics affecting reservoir ecology, particularly as the present study is the first to perform such an analysis for the Streževo Reservoir. It highlights the impacts of thermal stratification on biochemical processes, the seasonal variations in dissolved oxygen and phosphorus levels due to phytoplankton activity, and the influences of temperature on zooplankton diversity and abundance. Furthermore, it introduces the Zoo-IQ index as an innovative tool for assessing water quality through zooplankton analysis, emphasizing its relevance as an early indicator of ecological changes in freshwater systems. Moreover, this multi-faceted approach underscores the complexity of reservoir ecosystems and the importance of proactive management strategies to the mitigation of water quality fluctuations. This study underlines the need for continuous monitoring and proactive management strategies to address the aging of reservoirs. Full article
(This article belongs to the Section Sustainable Water Management)
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<p>Map of Streževo Reservoir (the investigated reservoir) and three sampling stations (S1, S2, and S3).</p>
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<p>Spatial and temporal values (±SE) for a zooplankton: (<b>a</b>) abundance and (<b>b</b>) biomass in the Streževo Reservoir. Abbreviations: Sp—spring; Su—summer; Au—autumn; I—epilimnion; VIII—metalimnion; XX—hypolimnion.</p>
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<p>Shannon index of diversity H′, and taxa number (S) of zooplankton in the Streževo Reservoir. Abbreviations: Sp—spring; Su—summer; Au—autumn; I—epilimnion; VIII—metalimnion; XX—hypolimnion.</p>
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<p>Nonmetric multidimensional scaling (nMDS) based on zooplankton assemblages at each sampling point in the Streževo Reservoir. Abbreviations: Sp—spring; Su—summer; Au—autumn; I—epilimnion; VIII—metalimnion; XX—hypolimnion; S1—sampling station at the inflow of the Šemnica River; S2—sampling station located at the central station in open water; and S3—sampling station near the dam. 2D stress = 0.1.</p>
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<p>Redundancy analysis (RDA) showing the zooplankton abundance and the influence of environmental factors. RDA1 = 22%; RDA2 = 17%. Abbreviations for zooplankton: AcanRobs—<span class="html-italic">Acanthocyclops robustus</span> (G. O. Sars, 1863); Asplanch—<span class="html-italic">Asplancha</span> sp.; Bosmina—; <span class="html-italic">Bosmina</span> sp.; BracFalc—<span class="html-italic">Brachionus falcatus</span> Zacharias, 1898; BracUrce—<span class="html-italic">Brachionus urceolaris</span> Müller, 1773; ChydSpha—<span class="html-italic">Chydorus sphaericus</span> (O. F. Müller, 1776); CyclOchr—<span class="html-italic">Cyclops ochridanus</span> Kiefer, 1932; CyclVicn—<span class="html-italic">Cyclops vicinus</span> Uljanin, 1875; CylpSp—<span class="html-italic">Cyclops</span> sp.; DaphCucl—<span class="html-italic">Daphnia cuculata</span> Sars, 1862; Diaphans—<span class="html-italic">Diaphanosoma</span> sp.; EudiGrac—<span class="html-italic">Eudiaptomus gracilis</span> (Sars, 1863); FinLong—<span class="html-italic">Filinia longiseta</span> (Ehrenberg, 1834); KellLong—<span class="html-italic">Kellicottia longispina</span> (Kellicott, 1879); KertCoch—<span class="html-italic">Keratella cochlearis</span> (Gosse, 1851); KertQuad—<span class="html-italic">Keratella quadrata</span> (Müller, 1786); Leptodor—<span class="html-italic">Leptodora kindtii</span> (Focke, 1844); Polyarth—<span class="html-italic">Polyarthra</span> sp.; MesoSp—<span class="html-italic">Mesocyclops</span> sp.; Trichocr—<span class="html-italic">Trichocerca</span> sp. Abbreviations for environmental factors: Chl—Chlorophyll <span class="html-italic">a</span> (μg L<sup>−1</sup>); OM—Dissolved organic matter (mg O<sub>2(Mn)</sub> L<sup>–1</sup>); Oxygen—Dissolved oxygen (mg L<sup>–1</sup>); T—Temperature (°C) TP—Total phosphorus (μg L<sup>–1</sup>); TN—Total nitrogen (μg L<sup>–1</sup>).</p>
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<p>Zoo-IQ index values in the Streževo Reservoir. Abbreviations: Sp—spring; Su—summer; Au—autumn; I—epilimnion; VIII—metalimnion; XX—hypolimnion. For A<sub>Zoo</sub>, B<sub>Zoo</sub>, MW<sub>Zoo</sub>, and R<sub>Clad</sub> and the range for water quality, please see the detailed description in Materials and Methods.</p>
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26 pages, 2749 KiB  
Article
Environmental Assessment Using Phytoplankton Diversity, Nutrients, Chlorophyll-a, and Trophic Status Along Southern Coast of Jeddah, Red Sea
by Bandar A. Al-Mur
J. Mar. Sci. Eng. 2025, 13(1), 29; https://doi.org/10.3390/jmse13010029 (registering DOI) - 29 Dec 2024
Viewed by 89
Abstract
The objective of this study is to better identify the state of eutrophication of coastal waters along the southern coast of the city of Jeddah in the Red Sea. Thirty-six samples from surface seawater were collected during the spring and autumn of 2021. [...] Read more.
The objective of this study is to better identify the state of eutrophication of coastal waters along the southern coast of the city of Jeddah in the Red Sea. Thirty-six samples from surface seawater were collected during the spring and autumn of 2021. Water temperature, pH, salinity, dissolved oxygen (DO), nutrients, and chlorophyll-a (Chl-a) content were examined as a guide of water quality indicators. The present data revealed low levels of Chl-a content (in the range of 0.11–0.24 µg L−1). The average concentrations of DIN (dissolved inorganic nitrogen) forms follow the order NO3-N > NH4-N ~ NO2-N (representing about 11.4–29.4% of the total nitrogen). To investigate the trophic status and water quality, numerical indicators were applied to the results of the analysis of chemical variables (NH4-N, NO3-N, and PO4-P) and the biological analysis (Chl-a) in the aqueous environment within the study area. These indicators are simplified based on the specialist, the non-specialist, the decision-maker, and the one responsible for managing the coastal areas. We also obtain through this method a single numerical value that expresses the state of the coastal waters. According to the analysis of phosphorus and nitrogen data and a trophic index (TRIX), the study area’s trophic status was determined as oligotrophic, due to low nutrient concentrations in the seawater. The current study identified a total of 58 species of phytoplankton comprised four classes in the investigated areas; Bacillariophyceae was the dominant algal class (Diatoms 30 species), followed by Chlorophyceae (9 species), Dinophyceae (11 species), and Cyanophyceae (8 species). Seasonally, spring recorded the highest value of total phytoplankton, recording a value of 251 × 103 cells/L with a percentage of 61%, while autumn recorded the lowest value of 186 × 103 cells/L with a percentage of 39%. Phytoplankton classes can be arranged in order of prevalence as follows: Bacillariophyceae >> Dinophyceae > Chlorophyceae > Cyanophyceae. Full article
(This article belongs to the Section Marine Environmental Science)
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<p>Sampling stations (1–18) of the study area.</p>
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<p>Spatial and temporal variations in physical and chemical parameters in the coastal region south of Jeddah in the Red Sea.</p>
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<p>Spatial and temporal changes in concentrations of NH<sub>4</sub>, NO<sub>2</sub>, NO<sub>3</sub>, TN, DIN, and DIN/TN in the coastal area south of Jeddah, Red Sea.</p>
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<p>Spatial and temporal changes in concentrations of chlorophyll-a (Chl-a), SiO<sub>4</sub>, reactive phosphate (PO<sub>4</sub>-P) and total phosphorus (TP).in the coastal area south of Jeddah, Red Sea.</p>
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<p>The abundance of phytoplankton composition in the coastal water of Jeddah during the period of 2022.</p>
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<p>The percentage abundance of phytoplankton composition in the coastal water of Jeddah during the period of 2022.</p>
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<p>Spatial assessments of the trophic status using the EUI based on NO<sub>3</sub>, NH<sub>4</sub>, and PO<sub>4</sub> concentration, eutrophication level (Ei), and TRIX values.</p>
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<p>The spatiotemporal trends of the diversity index and the number of planktonic species.</p>
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<p>The stoichiometric N/P ratios during the spring and autumn period in the coastal area south of Jeddah, Red Sea.</p>
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21 pages, 6383 KiB  
Article
Mapping the Incidence of Infestation by Neoechinorhynchus buttnerae (Acanthocephala) Parasitizing Colossoma macropomum Raised in Fish Farms and the Relationship with Zooplankton Ostracods and Copepods
by Vinicius Perez Pedroti, Jerônimo Vieira Dantas Filho, Átila Bezerra de Mira, Maria Mirtes de Lima Pinheiro, Bruna Lucieny Temponi Santos, Raniere Garcez Costa Sousa, Jucilene Braitenbach Cavali, Ed Johnny da Rosa Prado and Sandro de Vargas Schons
Vet. Sci. 2025, 12(1), 6; https://doi.org/10.3390/vetsci12010006 (registering DOI) - 29 Dec 2024
Viewed by 96
Abstract
This study investigated the abundance of zooplankton copepods and ostracods taxonomic groups in fish farms in Rondônia’s Vale do Jamari and Centro-Leste microregions during the rainy and dry seasons. It also analyzed the correlation between zooplanktons abundance and the presence of acanthocephalan parasites. [...] Read more.
This study investigated the abundance of zooplankton copepods and ostracods taxonomic groups in fish farms in Rondônia’s Vale do Jamari and Centro-Leste microregions during the rainy and dry seasons. It also analyzed the correlation between zooplanktons abundance and the presence of acanthocephalan parasites. Conducted in 41 fish farms between November 2021 and September 2023, the study included water, zooplankton, and fish samples from 196 Colossoma macropomum. The results showed that 95% of the farms had fish infected with Neoechinorhynchus buttnerae, with varying parasitism levels. Georeferencing revealed higher parasite densities in the municipalities of Ariquemes, Monte Negro, Machadinho do Oeste, and Buritis (Vale do Jamari), as well as Urupá, Ji-Paraná, Ouro Preto do Oeste, and Teixeirópolis (Centro-Leste), with clusters of heat islands in the latter group. Water quality parameters were suitable for raising C. macropomum. The presence of ostracods and copepods could serve as indicators of parasitic infestations, highlighting the importance of monitoring zooplankton and parasite communities. This approach is valuable for detecting changes in artificial ecosystems, such as fish farms, which could lead to significant long-term effects. Full article
(This article belongs to the Section Veterinary Microbiology, Parasitology and Immunology)
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<p>Geographic location of the fish farms visited in Rondônia; yellow municipalities located in the Central-East microregion, and green municipalities located in the Vale do Jamari microregion.</p>
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<p>Photomicrographs of copepod species—Thermocyclops decipiens (<b>A</b>), <span class="html-italic">Acanthocyclops</span> sp. (<b>B</b>), <span class="html-italic">Argyrodiaptomus</span> sp.(<b>C</b>), and <span class="html-italic">Argyrodiaptomus furcatus</span> (<b>D</b>)— and ostracod species—<span class="html-italic">Heterocypris</span> sp. (<b>E</b>), and <span class="html-italic">Heterocypris punctata</span> (<b>F</b>).</p>
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<p>Maps of the Kernel Diversity Index by microregions and by season of records of <span class="html-italic">Neoechinorhynchus buttnerae</span> (Acanthocephala) parasitizing <span class="html-italic">Colossoma macropomum</span> (<b>A</b>), abundance of Copepoda (<b>B</b>), and Ostracoda (<b>C</b>) in the water of fish farms.</p>
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<p>Maps of the Kernel Diversity Index by microregions and by season of records of <span class="html-italic">Neoechinorhynchus buttnerae</span> (Acanthocephala) parasitizing <span class="html-italic">Colossoma macropomum</span> (<b>A</b>), abundance of Copepoda (<b>B</b>), and Ostracoda (<b>C</b>) in the water of fish farms.</p>
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<p>Correlations between the parasitic load of acanthocephalans, the morphometric variations in the host fish, the seasonality and abundances of ostracods and copepods (<b>A</b>), and specific correlations for the microregions of Vale do Jamari (<b>B</b>) and Centro-Leste of Rondônia state (<b>C</b>).</p>
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<p>Correlations between weight (<b>A</b>), length (<b>B</b>), relative condition factor Kn (<b>C</b>), and parasite load (number of parasites/fish) of acanthocephalans parasitizing <span class="html-italic">Colossoma macropomum</span> in the Vale do Jamari and Centro-Leste microregions of Rondônia state.</p>
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<p>Correlations between health management practices and records of acanthocephalans parasitizing <span class="html-italic">Colossoma macropomum</span>.</p>
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<p>Correlations between dewormers/antiparasitics and the parasite intensity (no parasites, 1 to 300 and &gt;300) of acanthocephalans in <span class="html-italic">Colossoma macropomum</span> and water quality variables.</p>
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18 pages, 9391 KiB  
Article
Increased Contribution of Extended Vegetation Growing Season to Boreal Terrestrial Ecosystem GPP Enhancement
by Meng Yu, Yunfeng Cao, Jiaxin Tian and Boyu Ren
Remote Sens. 2025, 17(1), 83; https://doi.org/10.3390/rs17010083 (registering DOI) - 28 Dec 2024
Viewed by 234
Abstract
Rapid Arctic warming is driving significant changes in boreal vegetation phenology and productivity. The potentially asynchronous response of these processes could substantially alter the relative impacts of phenological shifts on variations in gross primary productivity (GPP), but this remains poorly understood. The objective [...] Read more.
Rapid Arctic warming is driving significant changes in boreal vegetation phenology and productivity. The potentially asynchronous response of these processes could substantially alter the relative impacts of phenological shifts on variations in gross primary productivity (GPP), but this remains poorly understood. The objective of this study is to quantify the impact of phenology extension on boreal ecosystem GPP changes across different periods from 1982 to 2018. To achieve this, we developed a statistical model that integrates vegetation phenology and physiology, and introduced a new metric, the Relative Increment Effect (RIE), to assess the contribution of phenology extension to GPP increase. Our analysis revealed that phenology extension became the dominant driver of GPP increment over time. Specifically, the overall RIE for boreal vegetation increased by 22% from the earlier period (P1: 1982–2000, 3.2) to the more recent period (P2: 2001–2018, 3.93). This increase was more pronounced for grass and shrub ecosystems. Spatial patterns showed that RIE increases were particularly concentrated at high latitudes, especially in northern Siberia. These findings suggested that phenology extension was playing an increasing role in regulating boreal ecosystem productivity, with significant implications for the boreal carbon budget under future warming scenarios. Full article
15 pages, 2495 KiB  
Technical Note
Asymmetric Distribution of Plasma Blobs During High Solar Activity in the Low- to Middle-Latitude Ionosphere
by Zhuo Huang, Jia Zhu, Weihua Luo, Zhengping Zhu, Guodong Jia and Shanshan Chang
Remote Sens. 2025, 17(1), 82; https://doi.org/10.3390/rs17010082 (registering DOI) - 28 Dec 2024
Viewed by 234
Abstract
Using the data from the first satellite of the Republic of China (ROCSAT-1) obtained during high-solar-activity periods (2000–2003), the distributions of plasma density enhancement (plasma blobs) with local time, season and longitude were investigated. Some new features of plasma blobs can be concluded: [...] Read more.
Using the data from the first satellite of the Republic of China (ROCSAT-1) obtained during high-solar-activity periods (2000–2003), the distributions of plasma density enhancement (plasma blobs) with local time, season and longitude were investigated. Some new features of plasma blobs can be concluded: (a) The distribution of plasma blobs shows remarkable seasonal and interhemispheric asymmetries, with the higher occurrence in June solstice months and in the winter hemisphere. (b) The occurrence of plasma blobs displays longitude dependence, more in the −180~−90°E, −60~0°E and 90~180°E longitude regions. (c) The seasonal and interhemispheric asymmetries of plasma blobs also depend on the longitude. Meridional wind plays an important role in the formation and evolution of low-latitude plasma blobs. Inclination and declination may control the longitudinal distribution of plasma blobs. Full article
22 pages, 11058 KiB  
Article
Predicting the Global Distribution of Nitraria L. Under Climate Change Based on Optimized MaxEnt Modeling
by Ke Lu, Mili Liu, Qi Feng, Wei Liu, Meng Zhu and Yizhong Duan
Plants 2025, 14(1), 67; https://doi.org/10.3390/plants14010067 (registering DOI) - 28 Dec 2024
Viewed by 246
Abstract
The genus of Nitraria L. are Tertiary-relict desert sand-fixing plants, which are an important forage and agricultural product, as well as an important source of medicinal and woody vegetable oil. In order to provide a theoretical basis for better protection and utilization of [...] Read more.
The genus of Nitraria L. are Tertiary-relict desert sand-fixing plants, which are an important forage and agricultural product, as well as an important source of medicinal and woody vegetable oil. In order to provide a theoretical basis for better protection and utilization of species in the Nitraria L., this study collected global distribution information within the Nitraria L., along with data on 29 environmental and climatic factors. The Maximum Entropy (MaxEnt) model was used to simulate the globally suitable distribution areas for Nitraria L. The results showed that the mean AUC value was 0.897, the TSS average value was 0.913, and the model prediction results were excellent. UV-B seasonality (UVB-2), UV-B of the lowest month (UVB-4), precipitation of the warmest quarter (bio18), the DEM (Digital Elevation Model), and annual precipitation (bio12) were the key variables affecting the distribution area of Nitraria L, with contributions of 54.4%, 11.1%, 8.3%, 7.4%, and 4.1%, respectively. The Nitraria L. plants are currently found mainly in Central Asia, North Africa, the neighboring Middle East, and parts of southern Australia and Siberia. In future scenarios, except for a small expansion of the 2030s scenario model Nitraria L., the potential suitable distribution areas showed a decreasing trend. The contraction area is mainly concentrated in South Asia, such as Afghanistan and Pakistan, North Africa, Libya, as well as in areas of low suitability in northern Australia, where there was also significant shrinkage. The areas of expansion are mainly concentrated in the Qinghai–Tibet Plateau to the Iranian plateau, and the Sahara Desert is also partly expanded. With rising Greenhouse gas concentrations, habitat fragmentation is becoming more severe. Center-of-mass migration results also suggest that the potential suitable area of Nitraria L. will shift northwestward in the future. This study can provide a theoretical basis for determining the scope of Nitraria L. habitat protection, population restoration, resource management and industrial development in local areas. Full article
(This article belongs to the Section Plant Response to Abiotic Stress and Climate Change)
18 pages, 1784 KiB  
Article
Trends in Escherichia coli and Klebsiella pneumoniae Urinary Tract Infections and Antibiotic Resistance over a 5-Year Period in Southeastern Gabon
by Yann Mouanga-Ndzime, Cyrille Bisseye, Neil-Michel Longo-Pendy, Michelle Bignoumba, Anicet-Clotaire Dikoumba and Richard Onanga
Antibiotics 2025, 14(1), 14; https://doi.org/10.3390/antibiotics14010014 (registering DOI) - 28 Dec 2024
Viewed by 338
Abstract
Background: Urinary tract infections (UTIs) are a substantial global health concern, exacerbated by the widespread use of antibiotics and leading to the development of multidrug-resistant strains. The aim of this study was to analyze the temporal patterns of Escherichia coli and Klebsiella pneumoniae UTIs [...] Read more.
Background: Urinary tract infections (UTIs) are a substantial global health concern, exacerbated by the widespread use of antibiotics and leading to the development of multidrug-resistant strains. The aim of this study was to analyze the temporal patterns of Escherichia coli and Klebsiella pneumoniae UTIs and antibiotic resistance, taking into account various sociodemographic, clinical, and climatic factors within the study population. Methods: A total of 3026 urine samples from patients of all ages were analyzed over a period of five years by standard microbiological methods. Climatic data for the study area were also collected. Univariate and multivariate logistic regression analyses were performed to measure the impact of sociodemographic, clinical and climatic parameters on the occurrence of UTIs. Results: The study showed a 31.4% prevalence of UTIs among the population. Notably, there was a significant increase in pyelonephritis between 2019 and 2023 (p < 0.01). Furthermore, a significant association was found between cystitis and the long dry season, as well as the short rainy season. Furthermore, Escherichia coli and Klebsiella pneumoniae exhibited resistance to beta-lactams, quinolones, and co-trimoxazole. The resistance of Escherichia coli isolated from cystitis to nitrofurantoin showed a significant increase over the years (p < 0.04). Principal component analysis (PCA) suggested that humidity may play a role in the emergence of multidrug-resistant strains of Escherichia coli and Klebsiella pneumoniae. Conclusions: UTIs show variability according to various sociodemographic, clinical, and climatic factors, with a higher risk of complications seen in individuals aged ≤ 17 years. It is important to note that cases of pyelonephritis have been increasing over time, with a noticeable seasonal variation. This study suggests that humidity may play a role in promoting antibiotic multidrug resistance in Escherichia coli and Klebsiella pneumoniae. Full article
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<p>Trend in urinary tract infections over 5 years. This plot displays the prevalence rates of cystitis and pyelonephritis across the annual data over five years.</p>
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<p>Seasonal prevalence of cystitis and pyelonephritis. The bar chart displays the prevalence rates (%) of cystitis and pyelonephritis across different seasons. The seasons are categorized as LD (long dry season), SD (short dry season), LR (long rainy season), and SR (short rainy season).</p>
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<p>Prevalence trends of <span class="html-italic">E. coli</span> and <span class="html-italic">K. pneumoniae</span> isolates across sociodemographic, seasonal and temporal parameters. This plot illustrates the prevalence rates of <span class="html-italic">E. coli</span> and <span class="html-italic">K. pneumoniae</span> isolates across various sociodemographic parameters (such as gender and age groups), seasonal factors, and annual data over five years.</p>
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<p>Antibiotic resistance profiles of <span class="html-italic">E. coli</span> and <span class="html-italic">K. pneumoniae</span> isolated from cystitis and pyelonephritis. This heatmap shows antibiotic resistance rates (%) for <span class="html-italic">E. coli</span> and <span class="html-italic">K. pneumoniae</span> isolates from cystitis and pyelonephritis. Resistance is presented for various antibiotic classes, with red indicating high resistance (up to 100%) and blue representing low resistance (close to 0%). Multidrug resistance (MDR) is displayed at the top.</p>
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<p>Principal component analysis of multidrug resistance and climatic factors. This figure illustrates the association between multidrug resistance and various climatic factors, including temperature, humidity, precipitation rate, number of rainy days, and number of stormy days.</p>
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<p>Trends in antibiotic resistance of interest in <span class="html-italic">E. coli</span> and <span class="html-italic">K. pneumoniae</span> isolated from UTIs over five years. This figure displays the trends in antibiotic resistance of <span class="html-italic">E. coli</span> and <span class="html-italic">K. pneumoniae</span> isolates from cystitis cases (<b>A</b>,<b>C</b>) and pyelonephritis cases (<b>B</b>,<b>D</b>) over the past five years, respectively. The data include resistance percentages for several antibiotics, showing how resistance levels have changed annually.</p>
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