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Search Results (28,994)

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21 pages, 5270 KiB  
Article
Three-Dimensional Object Recognition Using Orthogonal Polynomials: An Embedded Kernel Approach
by Aqeel Abdulazeez Mohammed, Ahlam Hanoon Al-sudani, Alaa M. Abdul-Hadi, Almuntadher Alwhelat, Basheera M. Mahmmod, Sadiq H. Abdulhussain, Muntadher Alsabah and Abir Hussain
Algorithms 2025, 18(2), 78; https://doi.org/10.3390/a18020078 (registering DOI) - 1 Feb 2025
Abstract
Computer vision seeks to mimic the human visual system and plays an essential role in artificial intelligence. It is based on different signal reprocessing techniques; therefore, developing efficient techniques becomes essential to achieving fast and reliable processing. Various signal preprocessing operations have been [...] Read more.
Computer vision seeks to mimic the human visual system and plays an essential role in artificial intelligence. It is based on different signal reprocessing techniques; therefore, developing efficient techniques becomes essential to achieving fast and reliable processing. Various signal preprocessing operations have been used for computer vision, including smoothing techniques, signal analyzing, resizing, sharpening, and enhancement, to reduce reluctant falsifications, segmentation, and image feature improvement. For example, to reduce the noise in a disturbed signal, smoothing kernels can be effectively used. This is achievedby convolving the distributed signal with smoothing kernels. In addition, orthogonal moments (OMs) are a crucial technique in signal preprocessing, serving as key descriptors for signal analysis and recognition. OMs are obtained by the projection of orthogonal polynomials (OPs) onto the signal domain. However, when dealing with 3D signals, the traditional approach of convolving kernels with the signal and computing OMs beforehand significantly increases the computational cost of computer vision algorithms. To address this issue, this paper develops a novel mathematical model to embed the kernel directly into the OPs functions, seamlessly integrating these two processes into a more efficient and accurate approach. The proposed model allows the computation of OMs for smoothed versions of 3D signals directly, thereby reducing computational overhead. Extensive experiments conducted on 3D objects demonstrate that the proposed method outperforms traditional approaches across various metrics. The average recognition accuracy improves to 83.85% when the polynomial order is increased to 10. Experimental results show that the proposed method exhibits higher accuracy and lower computational costs compared to the benchmark methods in various conditions for a wide range of parameter values. Full article
(This article belongs to the Section Algorithms for Multidisciplinary Applications)
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<p>Samples showing the effect of kernels on ant 3D objects using conventional convolution and OP-based convolution.</p>
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<p>Samples showing the effect of kernels on plane 3D objects using conventional convolution and OP-based convolution.</p>
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<p>Flow diagram of the proposed embedded kernel.</p>
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<p>Flow diagram of object recognition using the proposed technique.</p>
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<p>Samples of 3D objects extracted from McGill dataset.</p>
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15 pages, 6893 KiB  
Article
Effects of Closed Mouth vs. Exposed Teeth on Facial Expression Processing: An ERP Study
by Nicolas M. Brunet and Alexandra R. Ackerman
Behav. Sci. 2025, 15(2), 163; https://doi.org/10.3390/bs15020163 (registering DOI) - 1 Feb 2025
Abstract
The current study examines the neural mechanisms underlying facial recognition, focusing on how emotional expression and mouth display modulate event-related potential (ERP) waveforms. 42 participants categorized faces by gender in one of two experimental setups: one featuring full-face images and another with cropped [...] Read more.
The current study examines the neural mechanisms underlying facial recognition, focusing on how emotional expression and mouth display modulate event-related potential (ERP) waveforms. 42 participants categorized faces by gender in one of two experimental setups: one featuring full-face images and another with cropped faces presented against neutral gray backgrounds. The stimuli included 288 images balanced across gender, race/ethnicity, emotional expression (“Fearful”, “Happy”, “Neutral”), and mouth display (“closed mouth” vs. “open mouth with exposed teeth”). Results revealed that N170 amplitude was significantly greater for open-mouth (exposed teeth) conditions (p < 0.01), independent of emotional expression, and no interaction between emotional expression and mouth display was found. However, the P100 amplitude exhibited a significant interaction between these variables (p < 0.05). Monte Carlo simulations analyzing N170 latency differences showed that fearful faces elicited a faster response than happy and neutral faces, with a 2 ms delay unlikely to occur by chance (p < 0.01). While these findings challenge prior research suggesting that N170 is directly influenced by emotional expression, they also highlight the potential role of emotional intensity as an alternative explanation. This underscores the importance of further studies to disentangle these effects. This study highlights the critical need to control for mouth display when investigating emotional face processing. The results not only refine our understanding of the neural dynamics of face perception but also confirm that the brain processes fearful expressions more rapidly than happy or neutral ones. These insights offer valuable methodological considerations for future neuroimaging research on emotion perception. Full article
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<p>Stimulus Selection and Experimental Setup. (<b>A left</b>): Example stimuli used in Version 1, illustrating the experimental conditions based on the independent variables: emotional expression (“Fearful”, “Happy”, and “Neutral”) and mouth display condition (“closed mouth” and “open mouth with exposed teeth”). (<b>A right</b>): Example stimuli used in Version 2, which are the same as those in Version 1 but cropped and presented with a different background (see Methods for details). (<b>B</b>): Stimulus presentation and response task for both versions. A total of 288 stimuli are presented in random order. Participants use a button box to indicate whether the face is male or female. Faces are displayed for a minimum of 1 s and remain on screen until the participant responds or for a maximum of 4 s if no response is recorded. The intertrial interval is 1 s, during which a fixation cross appears at the center of the monitor. (<b>C</b>): Topographic map of sensor locations. This panel shows the topographic map of sensor locations used in the study. Results (<a href="#behavsci-15-00163-f002" class="html-fig">Figure 2</a> and <a href="#behavsci-15-00163-f003" class="html-fig">Figure 3</a>, and <a href="#behavsci-15-00163-t001" class="html-table">Table 1</a> and <a href="#behavsci-15-00163-t002" class="html-table">Table 2</a>) were derived from data averaged across the sensors located above the occipitotemporal scalp regions (highlighted in purple).</p>
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<p>ERP Waveforms Across Experimental Conditions. (<b>A</b>): Grand-mean ERP waveforms averaged across 19 participants (Version 1) and a cluster of occipitotemporal channels (see Methods and <a href="#behavsci-15-00163-f001" class="html-fig">Figure 1</a>C for electrode selection). Waveforms are split by emotional expression condition (“Neutral” in purple, “Happy” in red, and “Fearful” in cyan), with 96 trials per condition per participant. (<b>B</b>): Same data as (<b>A</b>), but split by mouth display condition (“closed mouth” in purple, “open mouth with exposed teeth” in cyan), with 144 trials per condition. (<b>C</b>,<b>D</b>): Equivalent data to (<b>A</b>,<b>B</b>), respectively, but from Version 2 of the experiment, with 23 participants. (<b>E</b>,<b>F</b>): Combined results across both experimental versions, pooling data from all 42 participants. (<b>E</b>) Aggregate results from (<b>A</b>,<b>C</b>), while (<b>F</b>) combines (<b>B</b>,<b>D</b>). Statistical testing of amplitude differences between experimental conditions was conducted using sliding paired <span class="html-italic">t</span>-tests (see Methods). Time intervals with statistically significant differences are indicated by vertical lines beneath the waveforms, color-coded as follows: yellow (<span class="html-italic">p</span> &lt; 0.05), orange (<span class="html-italic">p</span> &lt; 0.01), and red (<span class="html-italic">p</span> &lt; 0.001). For the independent variable “emotion” with three conditions (see <b>A</b>,<b>C</b>,<b>E</b>), pairwise comparisons were performed between conditions, resulting in three comparisons. The dotted vertical line in each panel indicates the timing of stimulus onset.</p>
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<p>Delay between ERP waveforms across emotional expression conditions. To explore the delay observed between the “Fearful” condition and the other two conditions (“Happy” and “Neutral”) within the time window highlighted by the dotted rectangle (copied from <a href="#behavsci-15-00163-f002" class="html-fig">Figure 2</a>E), Monte Carlo simulations with 10,000 iterations were performed (see Methods for details). The inset displays the results of these simulations as a histogram, with the observed delay of 2 ms indicated by a red vertical line.</p>
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24 pages, 10651 KiB  
Article
CLEAR: Multimodal Human Activity Recognition via Contrastive Learning Based Feature Extraction Refinement
by Mingming Cao, Jie Wan and Xiang Gu
Sensors 2025, 25(3), 896; https://doi.org/10.3390/s25030896 (registering DOI) - 1 Feb 2025
Abstract
Human activity recognition (HAR) has become a crucial research area for many applications, such as Healthcare, surveillance, etc. With the development of artificial intelligence (AI) and Internet of Things (IoT), sensor-based HAR has gained increasing attention and presents great advantages to existing work. [...] Read more.
Human activity recognition (HAR) has become a crucial research area for many applications, such as Healthcare, surveillance, etc. With the development of artificial intelligence (AI) and Internet of Things (IoT), sensor-based HAR has gained increasing attention and presents great advantages to existing work. Relying solely on existing labeled data may not adequately address the challenge of ensuring the model’s generalization ability to new data. The ’CLEAR’ method is designed to improve the accuracy of multimodal human activity recognition. This approach employs data augmentation, multimodal feature fusion, and contrastive learning techniques. These strategies are utilized to refine and extract highly discriminative features from various data sources, thereby significantly enhancing the model’s capacity to identify and classify diverse human activities accurately. CLEAR achieves high generalization performance on unknown datasets using only training data. Furthermore, CLEAR can be directly applied to various target domains without retraining or fine-tuning. Specifically, CLEAR consists of two parts. First, it employs data augmentation techniques in both the time and frequency domains to enrich the training data. Second, it optimizes feature extraction using attention-based multimodal fusion techniques and employs supervised contrastive learning to improve feature discriminability. We achieved accuracy rates of 81.09%, 90.45%, and 82.75% on three public datasets USC-HAD, DSADS, and PAMAP2, respectively. Additionally, when the training data are reduced from 100% to 20%, the model’s accuracy on the three datasets decreases by only about 5%, demonstrating that our model possesses strong generalization capabilities. Additionally, when the training data are reduced from 100% to 20%, the model’s accuracy on the three datasets decreases by only about 5%, demonstrating that our model possesses strong generalization capabilities. Full article
(This article belongs to the Section Physical Sensors)
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<p>(<b>a</b>) A person’s activity patterns vary throughout different time periods. (<b>b</b>) Different individuals exhibit varying ways of engaging in the same activity.</p>
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<p>Different individuals exhibit varying ways of engaging in the same activity.</p>
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<p>The Overall Framework of CLEAR. The upper half of the figure represents the training section, while the lower half represents the testing section.</p>
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<p>(<b>a</b>) Confusion matrix of dataset USC-HAD. (<b>b</b>) Confusion matrix of dataset DSADS. (<b>c</b>) Confusion matrix of dataset PAMAP2.</p>
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<p>(<b>a</b>) Confusion matrix of dataset USC-HAD. (<b>b</b>) Confusion matrix of dataset DSADS. (<b>c</b>) Confusion matrix of dataset PAMAP2.</p>
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<p>(<b>a</b>) A line graph showing the change in classification accuracy of DSADS dataset when the training data volume changes. (<b>b</b>) A line graph showing the change in classification accuracy of USC-HAD dataset when the training data volume changes. (<b>c</b>) A line graph showing the change in classification accuracy of PAMAP2 dataset when the training data volume changes.</p>
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<p>(<b>a</b>) A line graph showing the change in classification accuracy of DSADS dataset when the training data volume changes. (<b>b</b>) A line graph showing the change in classification accuracy of USC-HAD dataset when the training data volume changes. (<b>c</b>) A line graph showing the change in classification accuracy of PAMAP2 dataset when the training data volume changes.</p>
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<p>(<b>a</b>) This plot depicts the t-SNE visualization of DDLearn on the USC-HAD dataset. (<b>b</b>) This plot depicts the t-SNE visualization of CLEAR on the USC-HAD dataset. (<b>c</b>) This plot depicts the t-SNE visualization of DDLearn on the DSADS dataset. (<b>d</b>) This plot depicts the t-SNE visualization of CLEAR on the DSADS dataset. (<b>e</b>) This plot depicts the t-SNE visualization of DDLearn on the PAMAP2 dataset. (<b>f</b>) This plot depicts the t-SNE visualization of CLEAR on the PAMAP2 dataset.</p>
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<p>(<b>a</b>) It represents the effectiveness of CLEAR components on the DSADS dataset. (<b>b</b>) It represents the effectiveness of CLEAR components on the USC-HAD dataset. (<b>c</b>) It represents the effectiveness of CLEAR components on the PAMAP2 dataset. The components are as follows: frequency domain enhancement, feature fusion, and contrastive learning.</p>
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<p>Bar chart of classification accuracy on three public Datasets in %.</p>
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19 pages, 300 KiB  
Article
Determinants of Women Empowerment: Case of Refugee Women Living in Nairobi Kenya
by Judy Kaaria and Immaculate Kathomi Murithi
Economies 2025, 13(2), 35; https://doi.org/10.3390/economies13020035 (registering DOI) - 1 Feb 2025
Abstract
This study investigates the determinants of women empowerment among refugee women living in Nairobi, Kenya. First, the study constructs an index to examine empowerment drivers using data from the Refugee and Host Household Survey (RHHS) 2021. A fractional logit regression model was employed [...] Read more.
This study investigates the determinants of women empowerment among refugee women living in Nairobi, Kenya. First, the study constructs an index to examine empowerment drivers using data from the Refugee and Host Household Survey (RHHS) 2021. A fractional logit regression model was employed in the study. The results obtained show that the incidence of refugee women empowerment among refugees living in Nairobi was six percent. In addition, the study finds evidence that age; the gender of the household head; the education level of the refugee woman; employment status; and the education of the household head play substantial roles in enabling women empowerment. Conversely, marital statuses (divorced/separated/widowed and single/never married) and religious affiliations (Muslim) hinder women empowerment. Efforts geared towards improving wage employment and education are likely to empower refugee women. The study emphasizes the recognition of the role played by women in household income through care work. Full article
(This article belongs to the Special Issue Human Capital Development in Africa)
34 pages, 2238 KiB  
Review
Epigallocatechin Gallate (EGCG): Pharmacological Properties, Biological Activities and Therapeutic Potential
by Lucia Capasso, Luigi De Masi, Carmina Sirignano, Viviana Maresca, Adriana Basile, Angela Nebbioso, Daniela Rigano and Paola Bontempo
Molecules 2025, 30(3), 654; https://doi.org/10.3390/molecules30030654 (registering DOI) - 1 Feb 2025
Abstract
Epigallocatechin gallate (EGCG), the predominant catechin in green tea, comprises approximately 50% of its total polyphenol content and has garnered widespread recognition for its significant therapeutic potential. As the principal bioactive component of Camellia sinensis, EGCG is celebrated for its potent antioxidant, [...] Read more.
Epigallocatechin gallate (EGCG), the predominant catechin in green tea, comprises approximately 50% of its total polyphenol content and has garnered widespread recognition for its significant therapeutic potential. As the principal bioactive component of Camellia sinensis, EGCG is celebrated for its potent antioxidant, anti-inflammatory, cardioprotective, and antitumor properties. The bioavailability and metabolism of EGCG within the gut microbiota underscore its systemic effects, as it is absorbed in the intestine, metabolized into bioactive compounds, and transported to target organs. This compound has been shown to influence key physiological pathways, particularly those related to lipid metabolism and inflammation, offering protective effects against a variety of diseases. EGCG’s ability to modulate cell signaling pathways associated with oxidative stress, apoptosis, and immune regulation highlights its multifaceted role in health promotion. Emerging evidence underscores EGCG’s therapeutic potential in preventing and managing a range of chronic conditions, including cancer, cardiovascular diseases, neurodegenerative disorders, and metabolic syndromes. Given the growing prevalence of lifestyle-related diseases and the increasing interest in natural compounds, EGCG presents a promising avenue for novel therapeutic strategies. This review aims to summarize current knowledge on EGCG, emphasizing its critical role as a versatile natural bioactive agent with diverse clinical applications. Further exploration in both experimental and clinical settings is essential to fully unlock its therapeutic potential. Full article
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<p>The chemical structures of the four main catechins found in tea and their precursor.</p>
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<p>Absorption and metabolism of EGCG.</p>
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<p>Biological and therapeutic potential of EGCG.</p>
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<p>EGCG in cancer: potential for chemoprevention and therapy.</p>
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<p>Role of EGCG in signaling pathways involved in cancer.</p>
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15 pages, 740 KiB  
Article
The Application of the Vesikari and Modified Vesikari Severity Scores in Complicated Pediatric Gastroenteritis of Viral Origin: An Observational Study
by Maria Oana Săsăran, Cristina Oana Mărginean, Carmen Viorica Muntean, Ana Maria Pitea, Lidia Man, Alina Grama and Ana Maria Koller
J. Clin. Med. 2025, 14(3), 943; https://doi.org/10.3390/jcm14030943 (registering DOI) - 1 Feb 2025
Viewed by 39
Abstract
Background/Objectives: Viral gastroenteritis can have a potentially fatal outcome at young ages and the recognition of severe cases could be aided by clinically derived severity scores. Methods: This observational study intended to conduct a comparative assessment of the utility of the [...] Read more.
Background/Objectives: Viral gastroenteritis can have a potentially fatal outcome at young ages and the recognition of severe cases could be aided by clinically derived severity scores. Methods: This observational study intended to conduct a comparative assessment of the utility of the Vesikari and modified Vesikari score in the evaluation of viral gastroenteritis severity and for the possible prediction of the dehydration degree. A total number of 113 children diagnosed with gastroenteritis were retrospectively enrolled and divided based on viral etiology into group 1 (34 children with unknown viral etiology), group 2 (60 children with rotavirus) and group 3 (19 children with adenovirus). Results: The highest mean Vesikari and modified Vesikari scores were found in group 2 (p<0.01; p=0.01). A significant increase in liver enzymes was also identified in patients infected with rotavirus. The highest mean diarrhea, vomiting duration and body temperature were found in group 3 (p<0.01; p<0.01; p=0.02), as well as the highest mean inflammatory markers, such as C-reactive protein (CRP; p=0.01) and the erythrocyte sedimentation rate (p<0.01). Significant linear associations were found between pH, bicarbonate level, base excess and the Vesikari scores, whereas urea, CRP and aspartate aminotransferase levels were associated with both severity scores. ROC curve analysis revealed a significant correlation between the Vesikari scores and dehydration degree (p<0.01), with numeric cut-off values of 11.5 being proposed for the differentiation between mild and moderate gastroenteritis and 13.5 for the distinction between moderate and severe gastroenteritis. Conclusions: Both severity scores are useful in clinical settings, but more studies enrolling populations with various enteral infections could provide more insight into their etiology-based performance and reflection of paraclinical changes. Full article
23 pages, 2368 KiB  
Article
“No One Is Safe”: Agricultural Burnings, Wildfires and Risk Perception in Two Agropastoral Communities in the Puna of Cusco, Peru
by Rossi Taboada-Hermoza and Alejandra G. Martínez
Fire 2025, 8(2), 60; https://doi.org/10.3390/fire8020060 (registering DOI) - 1 Feb 2025
Viewed by 70
Abstract
By developing a conceptual framework that integrates the use of fire in agricultural activities, the occurrence of wildfires, and the perception of wildfire risk, this article examines the interplay among these three elements within both wet and dry Puna grasslands. The analysis focuses [...] Read more.
By developing a conceptual framework that integrates the use of fire in agricultural activities, the occurrence of wildfires, and the perception of wildfire risk, this article examines the interplay among these three elements within both wet and dry Puna grasslands. The analysis focuses on two peasant and agropastoral communities, Vilcabamba and Apachaco, both located in the Cusco region—an area with the highest incidence of wildfires in Peru. This study highlights the sociocultural significance and persistence of agricultural burnings within Puna agropastoral communities and the necessity of considering changes in agricultural activity, mutual aid systems, and communal institutions—particularly regarding land ownership—to understand the factors contributing to wildfire occurrence. Furthermore, it reveals the widespread recognition of wildfire risk among community members, who are acutely aware of both the likelihood and potential severity of wildfire events, while governmental policies aimed at addressing this hazard predominantly focus on raising awareness and enforcing bans on agricultural burning, with limited consideration of these complex sociocultural dynamics. Full article
(This article belongs to the Special Issue Biomass-Burning)
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<p>Conceptual model of fire use in agricultural activities and perception of wildfire risk in pastures. Based on Wisner B. et al. [<a href="#B54-fire-08-00060" class="html-bibr">54</a>], Paton D. et al. [<a href="#B55-fire-08-00060" class="html-bibr">55</a>], Oliveira S. et al. [<a href="#B56-fire-08-00060" class="html-bibr">56</a>], and Taboada Hermoza [<a href="#B19-fire-08-00060" class="html-bibr">19</a>].</p>
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<p>Distribution of wildfires in dry and wet Puna grasslands in Cusco.</p>
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<p>Map of the location of Vilcabamba and Apachaco communities in Cusco.</p>
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<p>Relationship between agricultural cycle activities and uses of fire in the dry Puna (DP) and wet Puna (WP).</p>
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15 pages, 639 KiB  
Article
Altered Monocyte Populations and Activation Marker Expression in Children with Autism and Co-Occurring Gastrointestinal Symptoms
by Rachel J. Moreno, Yasmin W. Azzam, Serena Eng, Destanie Rose and Paul Ashwood
Biomolecules 2025, 15(2), 207; https://doi.org/10.3390/biom15020207 (registering DOI) - 1 Feb 2025
Viewed by 79
Abstract
Autism spectrum disorder (ASD) is an early-onset neurodevelopmental condition that now impacts 1 in 36 children in the United States and is characterized by deficits in social communication, repetitive behaviors, and restricted interests. Children with ASD also frequently experience co-morbidities including anxiety and [...] Read more.
Autism spectrum disorder (ASD) is an early-onset neurodevelopmental condition that now impacts 1 in 36 children in the United States and is characterized by deficits in social communication, repetitive behaviors, and restricted interests. Children with ASD also frequently experience co-morbidities including anxiety and ADHD, and up to 80% experience gastrointestinal (GI) symptoms such as constipation, diarrhea, and/or abdominal pain. Systemic immune activation and dysregulation, including increased pro-inflammatory cytokines, are frequently observed in ASD. Evidence has shown that the innate immune system may be impacted in ASD, as altered monocyte gene expression profiles and cytokine responses to pattern recognition ligands have been observed compared to typically developing (TD) children. In humans, circulating monocytes are often categorized into three subpopulations—classical, transitional (or “intermediate”), and nonclassical monocytes, which can vary in functions, including archetypal inflammatory and/or reparative functions, as well as their effector locations. The potential for monocytes to contribute to immune dysregulation in ASD and its comorbidities has so far not been extensively studied. This study aims to determine whether these monocyte subsets differ in frequency in children with ASD and if the presence of GI symptoms alters subset distribution, as has been seen for T cell subsets. Whole blood from ASD children with (ASD+GI+) and without gastrointestinal symptoms (ASD+GI) and their TD counterparts was collected from children enrolled in the Childhood Autism Risk from Genetics and Environment (CHARGE) study. Peripheral blood mononuclear cells were isolated and stained for commonly used subset identifiers CD14 and CD16 as well as activation state markers CCR2, HLA-DR, PD-1, and PD-L1 for flow cytometry analysis. We identified changes in monocyte subpopulations and their expression of surface markers in children with ASD compared to TD children. These differences in ASD appear to be dependent on the presence or absence of GI symptoms. We found that the ASD+GI+ group have a different monocyte composition, evident in their classical, transitional, and nonclassical populations, compared to the ASD+GI and TD groups. Both the ASD+GI+ and ASD+GI groups exhibited greater frequencies of classical monocytes compared to the TD group. However, the ASD+GI+ group demonstrated lower frequencies of transitional and nonclassical monocytes than their ASD+GI and TD counterparts. CCR2+ classical monocyte frequencies were highest in the ASD+GI group. HLA-DR+ classical, transitional, and nonclassical monocytes were statistically comparable between groups, however, HLA-DR nonclassical monocyte frequencies were lower in both ASD groups compared to TD. The frequency of classical monocytes displaying exhaustion markers PD-1 and PD-L1 were increased in the ASD+GI+ group compared to ASD+GI and TD, suggesting potentially impaired ability for clearance of foreign pathogens or debris, typically associated with worsened inflammation. Taken together, the findings of differential proportions of the monocyte subpopulations and altered surface markers may explain some of the characteristics of immune dysregulation, such as in the gastrointestinal tract, observed in ASD. Full article
(This article belongs to the Special Issue Neuroimmune Interactions in Neuropsychiatric Diseases)
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<p>Monocyte subpopulation frequencies based on diagnosis and GI status. The percentage of live cells from each monocyte subpopulation—(<b>A</b>) classical (CD14<sup>+</sup>CD16<sup>−</sup>), (<b>B</b>) transitional (CD14<sup>+</sup>CD16<sup>+</sup>), and (<b>C</b>) nonclassical (CD14<sup>lo</sup>CD16<sup>+</sup>)—was identified using flow cytometry based on CD14 and CD16 expression in TD, ASD<sup>+</sup>GI<sup>−</sup>, and ASD<sup>+</sup>GI<sup>+</sup> groups. ROUT outlier removal (Q = 1%) was applied, and statistical significance between groups (<span class="html-italic">p</span> &lt; 0.05) was determined using ordinary one-way ANOVA and Tukey’s multiple comparisons test. Error bars represent SEM.</p>
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<p>CCR2 expression on monocyte subpopulations based on diagnosis and GI status. The percentage of CCR2-expressing cells from (<b>A</b>) classical (CD14<sup>+</sup>CD16<sup>−</sup>) and (<b>B</b>) transitional (CD14<sup>+</sup>CD16<sup>+</sup>) monocyte subpopulations was identified using flow cytometry in TD, ASD<sup>+</sup>GI<sup>−</sup>, and ASD<sup>+</sup>GI<sup>+</sup> groups. ROUT outlier removal (Q = 1%) was applied, and statistical significance between groups (<span class="html-italic">p</span> &lt; 0.05) was determined using ordinary one-way ANOVA and Tukey’s multiple comparisons test. Nonclassical (CD14<sup>lo</sup>CD16<sup>+</sup>) monocytes were excluded from the figure due to an insufficient number of captured events in the ASD<sup>+</sup>GI<sup>+</sup> group required for statistical analysis. Error bars represent SEM.</p>
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<p>HLA-DR expression on nonclassical monocyte populations based on ASD diagnosis and GI status. The percentage of HLA-DR non-expressing nonclassical (CD14<sup>lo</sup>CD16<sup>+</sup>) monocytes was identified using flow cytometry in the TD, ASD<sup>+</sup>GI<sup>−</sup>, and ASD<sup>+</sup>GI<sup>+</sup> groups. HLA-DR<sup>+</sup> classical (CD14<sup>+</sup>CD16<sup>−</sup>), transitional (CD14<sup>+</sup>CD16<sup>+</sup>), and nonclassical cells, as well as HLA-DR<sup>−</sup> classical and transitional cells, did not significantly differ across the three groups. ROUT outlier removal (Q = 1%) was applied, and statistical significance between groups (<span class="html-italic">p</span> &lt; 0.05) was determined using ordinary one-way ANOVA and Tukey’s multiple comparisons test. Error bars represent SEM.</p>
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<p>PD-1, PD-L1, and PD-1 PD-L1 co-expression on classical monocyte populations based on ASD diagnosis and GI status. The percentage of (<b>A</b>) PD-1, (<b>B</b>) PD-L1, and (<b>C</b>) PD-1 PD-L1 co-expressing cells from classical (CD14<sup>+</sup>CD16<sup>−</sup>) monocyte populations were identified using flow cytometry in TD, ASD<sup>+</sup>GI<sup>−</sup>, and ASD<sup>+</sup>GI<sup>+</sup> groups. ROUT outlier removal (Q = 1%) was applied, and statistical significance between groups (<span class="html-italic">p</span> &lt; 0.05) was determined using ordinary one-way ANOVA and Tukey’s multiple comparisons test. PD-1, PD-L1, and PD-1 PD-L1 co-expressing transitional (CD14<sup>+</sup>CD16<sup>+</sup>) monocytes did not significantly differ across the three groups. Nonclassical (CD14<sup>lo</sup>CD16<sup>+</sup>) monocytes were excluded from the figure due to an insufficient number of captured events in the ASD<sup>+</sup>GI<sup>+</sup> group required for statistical analysis. Error bars represent SEM.</p>
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19 pages, 2516 KiB  
Article
Application of Electronic Tongue for Detection and Classification of Lead Concentrations in Coal Mining Wastewater
by Jeniffer Katerine Carrillo Gómez, Laura Daniela Patiño Barrera and Cristhian Manuel Durán Acevedo
Environments 2025, 12(2), 41; https://doi.org/10.3390/environments12020041 (registering DOI) - 1 Feb 2025
Viewed by 106
Abstract
This study evaluates the potential of an electronic tongue (E-tongue) as an innovative and alternative method for detecting and classifying lead concentrations in wastewater generated by coal mining activities in North Santander, Colombia. The E-tongue aims to complement traditional environmental monitoring techniques with [...] Read more.
This study evaluates the potential of an electronic tongue (E-tongue) as an innovative and alternative method for detecting and classifying lead concentrations in wastewater generated by coal mining activities in North Santander, Colombia. The E-tongue aims to complement traditional environmental monitoring techniques with a more efficient and accurate solution. A total of 110 wastewater samples were collected from two locations at a coal mine in the municipality of Toledo: one inside the mine (Point 2) and another outside the mine (Point 1). This research involved the physicochemical analysis of parameters such as pH, biochemical oxygen demand (BOD), chemical oxygen demand (COD), total suspended solids (TSS), hardness, and alkalinity, conducted at the University of Pamplona’s laboratories. The integration of PCA with machine learning algorithms highlighted the E-tongue’s capability for the real-time, on-site detection and discrimination of lead concentrations in coal mining wastewater. Achieving a precision and accuracy above 90%, the SVM classifier outperformed alternative models such as the k-NN, Random Forest, Naïve Bayes, and Quadratic Discriminant Analysis. This demonstrates the system’s robustness and reliability in environmental monitoring, enabling the accurate classification of lead concentrations within the critical range of 0.05 to 1 ppm, essential for assessing contamination levels and ensuring water safety. These findings highlight the E-tongue system’s capability as a rapid, cost-effective tool for monitoring lead contamination in mining wastewater, presenting a viable alternative to conventional methods such as atomic absorption spectroscopy. Full article
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<p>Methodology established for lead quantification using electronic tongue.</p>
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<p>The response of the C110 electrode of the E-tongue to lead concentrations of 0.05 ppm and 1 ppm in wastewater.</p>
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<p>PCA plot of lead concentration (0.5 ppm to 100 ppm) categories in wastewater using E-tongue.</p>
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<p>Confusion matrix obtained from PCA-SVM classification model of lead concentrations in wastewater using E-tongue (C110 electrode).</p>
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<p>PCA analysis: Lead concentration (0.05 ppm to 1.0 ppm) categories in wastewater samples from Point 1 and Point 2, analyzed using E-tongue system.</p>
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<p>Confusion matrix obtained from PCA-RF classification model of lead concentrations in wastewater from Points 1 and 2 using E-tongue (C110 electrode).</p>
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14 pages, 2482 KiB  
Article
Selectively Blocking Small Conductance Ca2+-Activated K+ Channels Improves Cognition in Aged Mice
by Jessie Ong, H. Craig Heller and Elsa Pittaras
Biology 2025, 14(2), 149; https://doi.org/10.3390/biology14020149 (registering DOI) - 1 Feb 2025
Viewed by 124
Abstract
Aging is associated with decreased neuronal sensitivity and activity that creates deficits in cognitive processes, including learning, memory, motivation, general activity, and other behaviors. These effects are due in part to decreased intracellular Ca2+ homeostasis, increasing hyperpolarization of the resting potential in [...] Read more.
Aging is associated with decreased neuronal sensitivity and activity that creates deficits in cognitive processes, including learning, memory, motivation, general activity, and other behaviors. These effects are due in part to decreased intracellular Ca2+ homeostasis, increasing hyperpolarization of the resting potential in aged neurons and therefore decreasing their excitability. To reduce hyperpolarization in aged mice, we used apamin, a selective small conductance Ca2+-activated K+ (sKCa) channel blocker. By blocking the sKCa channels, apamin decreases the egress of the K+ out of the cell, reducing its hyperpolarization and causing it to be closer to threshold potential. As a result, neurons should be more sensitive to excitatory stimuli and more active. We evaluated the performance of aged mice in a selection of cognitive and behavioral tests prior to and after systemic applications of apamin or the vehicle saline. Apamin improved performance in short-term memory, increased attention to tasks, and decreased anhedonia. Apamin had no significant effect on long-term spatial and recognition memory, risk-taking behavior, sociability, and anxiety. Our results are compatible with the known effects of sKCa channel blockade on neuronal sensitivity and activity; however, these short-term effects were not reflected in longer-term alterations of neural plasticity responsible for long-term spatial and recognition memory or other more complex cognitive processes we evaluated. Full article
(This article belongs to the Section Behavioural Biology)
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<p>Experimental timeline overview. Injections were performed 30 min before each test. The same timeline was followed for the baseline before these two weeks of behavioral experiments with injections of saline or apamin.</p>
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<p>Schematic representation of the behavioral test: The Novel Object Recognition (NOR, (<b>A</b>)), T-maze (<b>B</b>), Social test (<b>C</b>), Nesting test (<b>D</b>), Novel Object Location (NOL, (<b>E</b>)) Elevated Plus Maze (EPM, (<b>F</b>)), Sucrose Preference test (<b>G</b>), and Openfield (<b>H</b>). TM = tested mouse, SM = stimulus mouse.</p>
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<p><b>Effect of apamin treatment on memory in mice.</b> Apamin improves spatial short-term memory in aged mice (<b>A</b>). Long-term memory evaluated by percentage duration with the novel object in NOR (<b>B</b>) and the object at the new location in the NOL test. Red square represents novel object, blue square are representative of habituated objects (<b>C</b>). T-test, * <span class="html-italic">p</span> &lt; 0.05, and *** <span class="html-italic">p</span> &lt; 0.001, significant difference between young, baseline, saline and/or apamin. Blue squares represent habituated objects.</p>
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<p><b>Effect of apamin on depressive-like behavior.</b> (<b>A</b>) Mean sucrose preference at baseline and after saline or apamin injections. Blue representative of water and pink representative of sucrose solution (<b>B</b>) Mean nesting scores at baseline and after saline or apamin injections. T-test, * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01 significant difference between baseline, saline, and/or apamin.</p>
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<p><b>Effects of apamin on social behavior in aged mice.</b> Sociability was quantified by (<b>A</b>) seconds spent in the social zone and (<b>B</b>) number of entries into the social zone. T-test or Wilcoxon test, * <span class="html-italic">p</span> &lt; 0.05 significant difference between baseline (<span class="html-italic">n</span> = 20), saline (<span class="html-italic">n</span> = 17), and/or apamin (<span class="html-italic">n</span> = 20).</p>
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<p>Effects of apamin on anxiety and risk taking. Risky decision making was quantified by the percentage of time spent in the central area of the Openfield (<b>A</b>), the duration and number of entries in the open arm of EPM (<b>B</b>,<b>C</b>) and in the risky zone of EPM (<b>D</b>,<b>E</b>) as well as the number of head dips in EPM (<b>F</b>). <b>T-test or Wilcoxon test, * <span class="html-italic">p</span> &lt; 0.05 significant difference between baseline (<span class="html-italic">n</span> = 19), saline (<span class="html-italic">n</span> = 17), and apamin (<span class="html-italic">n</span> = 19)</b>.</p>
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10 pages, 721 KiB  
Article
Food Intolerance and Allergy: Do They Have an Etiological Role in Idiopathic Granulomatous Mastitis?
by Muge Yurdacan, Berrin Papila, Basar Can Turgut, Hafize Uzun and Mehmet Velidedeoglu
J. Clin. Med. 2025, 14(3), 940; https://doi.org/10.3390/jcm14030940 (registering DOI) - 1 Feb 2025
Viewed by 116
Abstract
Background/Objectives: Despite its long-standing recognition, the etiopathogenesis of idiopathic granulomatous mastitis (IGM) remains poorly understood. This study aims to investigate the relationship between IGM and food intolerance, allergies, and immunological factors to shed light on its etiology. Materials and Methods: This case–control study [...] Read more.
Background/Objectives: Despite its long-standing recognition, the etiopathogenesis of idiopathic granulomatous mastitis (IGM) remains poorly understood. This study aims to investigate the relationship between IGM and food intolerance, allergies, and immunological factors to shed light on its etiology. Materials and Methods: This case–control study included 32 patients with IGM and 32 healthy women. In order to examine their potential relevance to allergy and immunology, serum interleukin (IL)-4, IL-4 receptor, histamine, and histamine-releasing factor (HRF) were measured by ELISA. Furthermore, serum IgG antibodies against specific food allergens were measured to evaluate food intolerance. Results: The patient group exhibited significantly higher intolerance values for lentils and curry compared to the control group (p = 0.023 and p = 0.012, respectively). Histamine (p < 0.001) and IL-4 (p = 0.003) levels were elevated in IGM patients compared to the control group, while HRF and IL-4R outcomes did not show any significant differences (p > 0.05). Conclusions: Elevated histamine and IL-4 levels may suggest the involvement of allergy and immunological factors in IGM’s etiopathogenesis. The integration of anti-histamine medications for IGM patients with elevated histamine levels could provide an alternative therapeutic strategy. Full article
(This article belongs to the Section Immunology)
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<p>Histamine, IL-4, IL-4R, and HRF values in IGM and control groups. Mann–Whitney U Test.</p>
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<p>Significant food intolerance differences between patient and control groups.</p>
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25 pages, 3685 KiB  
Article
Blind Source Separation Using Time-Delayed Dynamic Mode Decomposition
by Gyurhan Nedzhibov
Computation 2025, 13(2), 31; https://doi.org/10.3390/computation13020031 (registering DOI) - 1 Feb 2025
Viewed by 118
Abstract
Blind Source Separation (BSS) is a significant field of study in signal processing, with many applications in various fields such as audio processing, speech recognition, biomedical signal analysis, image processing and communication systems. Traditional methods, such as Independent Component Analysis (ICA), often rely [...] Read more.
Blind Source Separation (BSS) is a significant field of study in signal processing, with many applications in various fields such as audio processing, speech recognition, biomedical signal analysis, image processing and communication systems. Traditional methods, such as Independent Component Analysis (ICA), often rely on statistical independence assumptions, which may limit their performance in systems with significant temporal dynamics. This paper introduces an extension of the dynamic mode decomposition (DMD) approach by using time-delayed coordinates to implement BSS. Time-delay embedding enhances the capability of the method to handle complex, nonstationary signals by incorporating their temporal dependencies. We validate the approach through numerical experiments and applications, including audio signal separation, image separation and EEG artifact removal. The results demonstrate that modification achieves superior performance compared to conventional techniques, particularly in scenarios where sources exhibit dynamic coupling or non-stationary behavior. Full article
(This article belongs to the Special Issue Mathematical Modeling and Study of Nonlinear Dynamic Processes)
28 pages, 6569 KiB  
Article
A New Efficient Hybrid Technique for Human Action Recognition Using 2D Conv-RBM and LSTM with Optimized Frame Selection
by Majid Joudaki, Mehdi Imani and Hamid R. Arabnia
Technologies 2025, 13(2), 53; https://doi.org/10.3390/technologies13020053 (registering DOI) - 1 Feb 2025
Viewed by 207
Abstract
Recognizing human actions through video analysis has gained significant attention in applications like surveillance, sports analytics, and human–computer interaction. While deep learning models such as 3D convolutional neural networks (CNNs) and recurrent neural networks (RNNs) deliver promising results, they often struggle with computational [...] Read more.
Recognizing human actions through video analysis has gained significant attention in applications like surveillance, sports analytics, and human–computer interaction. While deep learning models such as 3D convolutional neural networks (CNNs) and recurrent neural networks (RNNs) deliver promising results, they often struggle with computational inefficiencies and inadequate spatial–temporal feature extraction, hindering scalability to larger datasets or high-resolution videos. To address these limitations, we propose a novel model combining a two-dimensional convolutional restricted Boltzmann machine (2D Conv-RBM) with a long short-term memory (LSTM) network. The 2D Conv-RBM efficiently extracts spatial features such as edges, textures, and motion patterns while preserving spatial relationships and reducing parameters via weight sharing. These features are subsequently processed by the LSTM to capture temporal dependencies across frames, enabling effective recognition of both short- and long-term action patterns. Additionally, a smart frame selection mechanism minimizes frame redundancy, significantly lowering computational costs without compromising accuracy. Evaluation on the KTH, UCF Sports, and HMDB51 datasets demonstrated superior performance, achieving accuracies of 97.3%, 94.8%, and 81.5%, respectively. Compared to traditional approaches like 2D RBM and 3D CNN, our method offers notable improvements in both accuracy and computational efficiency, presenting a scalable solution for real-time applications in surveillance, video security, and sports analytics. Full article
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<p>Overview of the proposed action recognition with respect to data dimension changes throughout the network. The pipeline of the proposed method, including preprocessed video frames, smart frame selection, 2D Conv-RBM for spatial feature extraction, LSTM for temporal modeling, and a fully connected layer for action classification.</p>
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<p>Overview of the smart frame selection mechanism adapted from [<a href="#B38-technologies-13-00053" class="html-bibr">38</a>]. This figure illustrates the process of evaluating individual and relational frame importance using both single-frame and global selectors. It showcases the calculation of <span class="html-italic">δ<sub>i</sub></span> and <span class="html-italic">γ<sub>i</sub></span>, combining them to score frame importance and selecting the top <span class="html-italic">n</span> frames based on these scores. The method reduces the number of frames passed to the network while retaining those most critical for action recognition.</p>
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<p>2D Conv-RBM reconstruction error for all datasets.</p>
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<p>Extended confusion matrix for KTH (<b>a</b>) and UCF Sports (<b>b</b>) datasets. This visualization provides a comprehensive assessment of classifier performance, including class-level recall (right column), precision, and F1 score (bottom rows). Darker diagonal elements indicate strong correct predictions, while off-diagonal color contrast reveals false positives and false negatives.</p>
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<p>Extended confusion matrix for the HMDB51 dataset. Logarithmic scaling enhances visibility across all value ranges, offering insights into both overall accuracy and class-specific performance.</p>
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<p>Scaled mean of LSTM training losses and accuracies across all datasets.</p>
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<p>Failure case analysis for the KTH dataset. Examples of misclassifications where the model predicted walking as jogging and jogging as walking, illustrating the challenges in distinguishing between actions with similar motion dynamics and overlapping spatial–temporal features.</p>
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<p>Failure case analysis for the UCF Sports dataset. Examples of misclassifications where the model predicted skateboarding as walking and walking as skateboarding, emphasizing the challenges posed by visually complex actions with overlapping spatial features and varied contextual backgrounds.</p>
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17 pages, 2289 KiB  
Article
Building Footprint Identification Using Remotely Sensed Images: A Compressed Sensing-Based Approach to Support Map Updating
by Rizwan Ahmed Ansari, Rakesh Malhotra and Mohammed Zakariya Ansari
Geomatics 2025, 5(1), 7; https://doi.org/10.3390/geomatics5010007 (registering DOI) - 31 Jan 2025
Viewed by 282
Abstract
Semantic segmentation of remotely sensed images for building footprint recognition has been extensively researched, and several supervised and unsupervised approaches have been presented and adopted. The capacity to do real-time mapping and precise segmentation on a significant scale while considering the intrinsic diversity [...] Read more.
Semantic segmentation of remotely sensed images for building footprint recognition has been extensively researched, and several supervised and unsupervised approaches have been presented and adopted. The capacity to do real-time mapping and precise segmentation on a significant scale while considering the intrinsic diversity of the urban landscape in remotely sensed data has significant consequences. This study presents a novel approach for delineating building footprints by utilizing the compressed sensing and radial basis function technique. At the feature extraction stage, a small set of random features of the built-up areas is extracted from local image windows. The random features are used to train a radial basis neural network to perform building classification; thus, learning and classification are carried out in the compressed sensing domain. By virtue of its ability to represent characteristics in a reduced dimensional space, the scheme shows promise in being robust in the face of variability inherent in urban remotely sensed images. Through a comparison of the proposed method with numerous state-of-the-art approaches utilizing remotely sensed data of different spatial resolutions and building clutter, we establish its robustness and prove its viability. Accuracy assessment is performed for segmented footprints, and comparative analysis is carried out in terms of intersection over union, overall accuracy, precision, recall, and F1 score. The proposed method achieved scores of 93% in overall accuracy, 90.4% in intersection over union, and 91.1% in F1 score, even when dealing with drastically different image features. The results demonstrate that the proposed methodology yields substantial enhancements in classification accuracy and decreases in feature dimensionality. Full article
21 pages, 2621 KiB  
Article
Sunflower Oil Fortified with Vitamins D and A and Sunflower Lecithin Ameliorated Scopolamine-Induced Cognitive Dysfunction in Mice and Exploration of the Underlying Protective Pathways
by Xue Tang, Chengkai Zhu, Tristan C. Liu, Rongxiang Zhu, Guoliang Deng, Peng Zhou and Dasong Liu
Nutrients 2025, 17(3), 553; https://doi.org/10.3390/nu17030553 (registering DOI) - 31 Jan 2025
Viewed by 232
Abstract
The incidence of cognitive disorders is increasing globally, with a reported prevalence of over 50 million individuals affected, and current interventions offer limited efficacy. This study investigates the effects of sunflower oil fortified with sunflower lecithin, vitamin D, and vitamin A on scopolamine-induced [...] Read more.
The incidence of cognitive disorders is increasing globally, with a reported prevalence of over 50 million individuals affected, and current interventions offer limited efficacy. This study investigates the effects of sunflower oil fortified with sunflower lecithin, vitamin D, and vitamin A on scopolamine-induced cognitive dysfunction in mice and explores the underlying mechanisms. The incidence of cognitive disorders, such as Alzheimer’s disease, is increasing yearly, and current interventions offer limited efficacy. Therefore, this research aims to evaluate the cognitive improvement effects of the three added functional factors on mice with learning and memory impairments, along with the associated molecular mechanisms. Behavioral tests, biochemical assays, and real-time quantitative polymerase chain reaction (RT-qPCR) were utilized to examine the intervention effects of these functional factors on scopolamine-induced cognitive impairment in mice. The results revealed that the groups treated with sunflower lecithin and vitamin D significantly enhanced the mice’s exploratory behavior, working memory, and spatial memory, with increases of 1.6 times and 4.5 times, respectively, in the open field and novel object recognition tests (VD group). Additionally, these treatments reduced levels of inflammatory markers and IL-6, increased antioxidant GSH levels, and decreased oxidative stress marker MDA levels, with all effects showing significant differences (p < 0.01). The effects were further enhanced when vitamin A was combined with these treatments. Transcriptomic analysis demonstrated that the intervention groups had markedly improved learning and memory abilities through upregulation of key gene expression levels in the PI3K-AKT signaling pathway, cholinergic pathway, and folate biosynthesis pathway. These findings provide a theoretical basis for the development of nutritionally fortified edible oils with added sunflower lecithin, vitamin D, and vitamin A, which may help prevent and ameliorate cognitive disorders. Full article
(This article belongs to the Section Nutrition and Public Health)
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