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23 pages, 7572 KiB  
Article
Arabic Temporal Common Sense Understanding
by Reem Alqifari, Hend Al-Khalifa and Simon O’Keefe
Computation 2025, 13(1), 5; https://doi.org/10.3390/computation13010005 (registering DOI) - 28 Dec 2024
Viewed by 37
Abstract
Natural language understanding (NLU) includes temporal text understanding, which can be complex and encompasses temporal common sense understanding. There are many challenges in comprehending common sense within a text. Currently, there is a limited number of datasets containing temporal common sense in English [...] Read more.
Natural language understanding (NLU) includes temporal text understanding, which can be complex and encompasses temporal common sense understanding. There are many challenges in comprehending common sense within a text. Currently, there is a limited number of datasets containing temporal common sense in English and there is an absence of such datasets specifically for the Arabic language. In this study, an Arabic dataset was constructed based on an available English dataset. This dataset is considered a valuable resource for the Arabic community. Consequently, different multilingual pre-trained language models (PLMs) were applied to both the English and new Arabic datasets. Based on this, the effectiveness of these models in Arabic and English is compared and discussed. After analyzing the errors, a new categorization of errors was proposed. Finally, the ability of the PLMs to understand the input text and predict temporal features was evaluated. Through this detailed categorization of errors and classification of temporal elements, this study establishes a comprehensive framework aimed at clarifying the specific challenges encountered by PLMs in temporal common sense understanding (TCU). This methodology underscores the urgent need for further research on PLMs’ capabilities for TCU tasks. Full article
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<p>Example of a TCU challenge showing a scenario where the model fails to validate the correct answer. The table highlights the scenario description, posed question, provided candidate answers, the correct label (marked with a ✔), and the model’s incorrect prediction (marked with a ✕). This example illustrates the limitations of the model’s temporal commonsense reasoning, emphasizing the need for better training or enhanced datasets tailored for temporal understanding.</p>
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<p>Percentage of the unique question–answer pairs in each temporal category.</p>
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<p>Sample of the dataset. Each row targets one temporal aspect from the five aspects covered by the original dataset. An example context for each aspect is provided from both the English and Arabic datasets. The English column is from the MC-TACO dataset and includes five different contexts, each representing one aspect. For each context, the question is provided along with all candidate answers, with the correct answers in bold. Note that there may be more than one correct answer for a question, and the number of answers for each question varies. The Arabic column is from the translated dataset.</p>
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<p>Model results: F1 score for each temporal aspect.</p>
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<p>Predictions of AraBERT vs. CAMeLBERT.</p>
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<p>The results of TCU and temporal classification for Arabic and English.</p>
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<p>Results of Arabic temporal classification in comparison with TCU.</p>
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35 pages, 659 KiB  
Article
Technological Convergence of Blockchain and Artificial Intelligence: A Review and Challenges
by Nakhoon Choi and Heeyoul Kim
Electronics 2025, 14(1), 84; https://doi.org/10.3390/electronics14010084 (registering DOI) - 27 Dec 2024
Viewed by 237
Abstract
Blockchain and artificial intelligence are two of the most prominent technologies in computer science today and have attracted considerable attention from various research communities. Recently, several initiatives have been launched to explore the combination of these two pioneering technologies. The main goal is [...] Read more.
Blockchain and artificial intelligence are two of the most prominent technologies in computer science today and have attracted considerable attention from various research communities. Recently, several initiatives have been launched to explore the combination of these two pioneering technologies. The main goal is to combine the data integrity, privacy, and decentralization properties of blockchain with the ability of artificial intelligence to process, analyze, predict, and refine massive data sets. The combination of blockchain and AI technologies is expected to address key challenges in the digital realm, such as data security, transparency, and streamlined decision-making. However, there is a problem that many studies have focused on the advancement of a single technology as the main perspective. To overcome these recent research limitations, we provide a broad view of the combination of blockchain and artificial intelligence and analyze the limitations of existing research and their causes. Furthermore, we identify challenges and attempts to be addressed through this analysis. The analysis in this paper is organized into a comprehensive section dedicated to the application of artificial intelligence in blockchain and vice versa. Based on our analysis, we identify existing challenges and propose a novel framework for researchers to overcome these limitations, thus expanding new research opportunities. Full article
(This article belongs to the Special Issue Feature Papers in "Computer Science & Engineering", 2nd Edition)
15 pages, 578 KiB  
Article
Exploiting Extrinsic Information for Serial MAP Detection by Utilizing Estimator in Holographic Data Storage Systems
by Thien An Nguyen and Jaejin Lee
Appl. Sci. 2025, 15(1), 139; https://doi.org/10.3390/app15010139 (registering DOI) - 27 Dec 2024
Viewed by 215
Abstract
In the big data era, data are created in huge volume. This leads to the development of storage devices. Many technologies are proposed for the next generation of storage fields. However, among them, holographic data storage (HDS) has attracted much attention and has [...] Read more.
In the big data era, data are created in huge volume. This leads to the development of storage devices. Many technologies are proposed for the next generation of storage fields. However, among them, holographic data storage (HDS) has attracted much attention and has been introduced as the promising candidate to meet the increasing demand for capacity and speed. For signal processing, HDS faces two major challenges: inter-page interference (IPI) and two-dimensional (2D) interference. To access the IPI problem, we can use balanced coding, which converts user data into an intensity level with uniformly distributed values for each page. For 2D interference, we can use the equalizer and detection to mitigate the 2D interference. However, the often-used equalizer and detection are methods in wireless communication and only handle the one-dimensional (1D) signal. Thus, we can combine the equalizer, detection, and estimator to reduce 2D interference into 1D interference. In this paper, we proposed a combined model using serial maximum a posteriori (MAP) detection and estimator to improve the detection of HDS systems. In our proposed model, instead of using an estimator with the Viterbi algorithm to predict the upper–lower interference (UPI) or left–right interference (LRI) and converting the received signal into 1D ISI, we used the estimator to predict the extrinsic information for serial MAP detection. This preserves the 2D information in the received signal in serial MAP detection and improves the detection of serial MAP detection by extrinsic information. The simulation results demonstrate that our proposed model significantly improves the bit-error rate (BER) performance compared to previous studies. Full article
29 pages, 2253 KiB  
Review
Impact of Abiotic Stressors on Soil Microbial Communities: A Focus on Antibiotics and Their Interactions with Emerging Pollutants
by Abdul Rashid P. Rasheela, Muhammad Fasih Khalid, Dana A. Abumaali, Juha M. Alatalo and Talaat Ahmed
Soil Syst. 2025, 9(1), 2; https://doi.org/10.3390/soilsystems9010002 - 26 Dec 2024
Viewed by 571
Abstract
Soil is a complex and dynamic ecosystem containing a diverse array of microorganisms, and plays a crucial and multifaceted role in various functions of the ecosystem. Substantial fluctuations in the environmental conditions arise from diverse global changes. The microbial shifts in the soil [...] Read more.
Soil is a complex and dynamic ecosystem containing a diverse array of microorganisms, and plays a crucial and multifaceted role in various functions of the ecosystem. Substantial fluctuations in the environmental conditions arise from diverse global changes. The microbial shifts in the soil in concordance with the changing environmental factors, or a combination of these factors, are of high significance. Exploring the contribution of global change drivers to the microbial community to improve the predictions of the response of the microbial community to the functioning of the ecosystem is of prime importance. Promoting the health of soil microorganisms maintains the overall health and fertility of the soil, which in turn supports the health of terrestrial ecosystems and agricultural systems. The current review aims to assemble different abiotic factors or stressors that exist in the environment that affect the microbial community. More focus will be given to one of the stressors—antibiotics, a recent emerging pollutant. The effects on the soil microbial community and the future of soil health due to the presence of antibiotics will be addressed. The scope of the interaction of antibiotics with other pollutants like plastics and heavy metals (HMs) will be examined. Full article
(This article belongs to the Special Issue Microbial Community Structure and Function in Soils)
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<p>Factors contributing to soil health.</p>
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<p>Results generated on Scopus on analyzing the documents from the year 2000 to 2023 on the topic “environmental factors affecting soil microorganisms”.</p>
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<p>The abundance and diversity of common living organisms found in the soil.</p>
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<p>Major sources and pathways of antibiotic contamination release residues into soil and water, increasing pollution risk. The potential movement between these ecosystems highlights the interconnectedness of these ecosystems and the risk of antibiotic pollution in both environments.</p>
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<p>Fate of antibiotics in the soil, depicting the cycle of antibiotics in different environmental compartments through the application of manure.</p>
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<p>Antibiotic use leads to ineffective treatments. The evolution of bacteria into “superbugs” takes place through repeated antibiotic exposure (indicated by the red arrow). The lightning symbols represent the emergence of resistance mechanisms through their interaction with other pollutants, ultimately leading to antibiotic resistance and ineffective treatments.</p>
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24 pages, 5160 KiB  
Article
Payload State Prediction Based on Real-Time IoT Network Traffic Using Hierarchical Clustering with Iterative Optimization
by Hao Zhang, Jing Wang, Xuanyuan Wang, Kai Lu, Hao Zhang, Tong Xu and Yan Zhou
Sensors 2025, 25(1), 73; https://doi.org/10.3390/s25010073 - 26 Dec 2024
Viewed by 221
Abstract
IoT (Internet of Things) networks are vulnerable to network viruses and botnets, while facing serious network security issues. The prediction of payload states in IoT networks can detect network attacks and achieve early warning and rapid response to prevent potential threats. Due to [...] Read more.
IoT (Internet of Things) networks are vulnerable to network viruses and botnets, while facing serious network security issues. The prediction of payload states in IoT networks can detect network attacks and achieve early warning and rapid response to prevent potential threats. Due to the instability and packet loss of communications between victim network nodes, the constructed protocol state machines of existing state prediction schemes are inaccurate. In this paper, we propose a network payload predictor called IoTGuard, which can predict the payload states in IoT networks based on real-time IoT network traffic. The steps of IoTGuard are briefly as follows: Firstly, the application-layer payloads between different nodes are extracted through a module of network payload separation. Secondly, the classification of payload state within network flows is obtained via a payload extraction module. Finally, the predictor of payload state in a network is trained on a payload set, and these payloads have state labels. Experimental results on the Mozi botnet dataset show that IoTGuard can predict the state of payloads in IoT networks more accurately while ensuring execution efficiency. IoTGuard achieves an accuracy of 86% in network payload prediction, which is 8% higher than the state-of-the-art method NetZob, and the training time is reduced by 52.8%. Full article
(This article belongs to the Special Issue IoT Network Security (Second Edition))
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<p>TCP message state machine.</p>
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<p>The two clusters formed after payload state clustering.</p>
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<p>Timing sequence of UDP datagrams from a pair of Mozi zombie nodes. The load type marked in red are out-of-order fields affected by network jitter.</p>
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<p>Wireshark parses the Mozi communication payload. The red box is the datagram type UDP packet.</p>
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<p>Communication process between Mozi nodes when the network is poor.</p>
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<p>State machine generated by bad network conditions. The serial numbers in the diagram label the state transitions in chronological order.</p>
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<p>Mozi communication payload clustering results.</p>
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<p>P2P network payload status prediction scheme framework. The serial numbers in the figure mark the steps in chronological order.</p>
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<p>Application layer network payload extraction process.</p>
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<p>Payload status extraction process. The serial numbers in the figure mark the steps in chronological order.</p>
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<p>Payload clustering process.</p>
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<p>Process of generating payload status predictor. The serial numbers in the figure mark the steps in chronological order.</p>
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<p>Clustering effect of adjusting the relationship between hierarchical clustering parameters and ARI coefficients.</p>
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<p>Corresponding training time using autoencoders with different layers.</p>
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<p>The effect of feature vector extraction using autoencoders with different layers.</p>
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<p>Relationship between initial parameters of hierarchical clustering and ARI coefficient.</p>
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29 pages, 2972 KiB  
Review
Enhancing the Resilience of Agroecosystems Through Improved Rhizosphere Processes: A Strategic Review
by Waleed Asghar, Kelly D. Craven, Jacob R. Swenson, Ryota Kataoka, Ahmad Mahmood and Júlia Gomes Farias
Int. J. Mol. Sci. 2025, 26(1), 109; https://doi.org/10.3390/ijms26010109 - 26 Dec 2024
Viewed by 296
Abstract
As farming practices evolve and climate conditions shift, achieving sustainable food production for a growing global population requires innovative strategies to optimize environmentally friendly practices and minimize ecological impacts. Agroecosystems, which integrate agricultural practices with the surrounding environment, play a vital role in [...] Read more.
As farming practices evolve and climate conditions shift, achieving sustainable food production for a growing global population requires innovative strategies to optimize environmentally friendly practices and minimize ecological impacts. Agroecosystems, which integrate agricultural practices with the surrounding environment, play a vital role in maintaining ecological balance and ensuring food security. Rhizosphere management has emerged as a pivotal approach to enhancing crop yields, reducing reliance on synthetic fertilizers, and supporting sustainable agriculture. The rhizosphere, a dynamic zone surrounding plant roots, hosts intense microbial activity fueled by root exudates. These exudates, along with practices such as green manure application and intercropping, significantly influence the soil’s microbial community structure. Beneficial plant-associated microbes, including Trichoderma spp., Penicillium spp., Aspergillus spp., and Bacillus spp., play a crucial role in improving nutrient cycling and promoting plant health, yet their interactions within the rhizosphere remain inadequately understood. This review explores how integrating beneficial microbes, green manures, and intercropping enhances rhizosphere processes to rebuild microbial communities, sequester carbon, and reduce greenhouse gas emissions. These practices not only contribute to maintaining soil health but also foster positive plant–microbe–rhizosphere interactions that benefit entire ecosystems. By implementing such strategies alongside sound policy measures, sustainable cropping systems can be developed to address predicted climate challenges. Strengthening agroecosystem resilience through improved rhizosphere processes is essential for ensuring food security and environmental sustainability in the future. In conclusion, using these rhizosphere-driven processes, we could develop more sustainable and resilient agricultural systems that ensure food security and environmental preservation amidst changing climate situations. Full article
(This article belongs to the Special Issue Plant Pathogen Interactions: 2nd Edition)
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<p>The rhizosphere is an active region where plant roots engage with soil microorganisms. Root exudates affect microbial activity, augmenting nutrient cycling, promoting plant growth, increasing enzyme production, and benefiting soil health. Beneficial microbes enhance nutrient absorption, improve the structure of the soil, and safeguard plants against diseases; hence, they play a vital role in the stability of agroecosystems.</p>
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<p>Soils in agroecosystems serve as a primary nutrient reservoir for crops, supplying necessary nutrients and water while also functioning as the most significant microbial reproductive bank for the rhizosphere microbiome. The plant offers many microhabitats, including both the endosphere and rhizosphere, for the development of the rhizosphere and plant microbiome. Beneficial plant–microbe interactions facilitate the rhizosphere microbiome’s services. Microbiome services improve plant growth, stress tolerance, and disease resistance, as well as host resilience to abiotic challenges such as cold, salinity, and drought, resulting in biomass production increasing up to fourfold and enhancing rhizosphere processes through their direct and indirect mechanisms. We created the conceptual diagram to provide an overview of rhizosphere microbiome services.</p>
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<p>Various green manures serve to improve soil health. The diagram illustrates the roles of leguminous and non-leguminous green manures in agroecosystems. Leguminous green manures, including clover, Hairy Vetch, and peas, improve soil health chiefly by facilitating biological nitrogen fixation; hence, they diminish the reliance on synthetic fertilizers and augment nitrogen availability for succeeding crops. Non-leguminous green manures, including mustard, rye, and radish, enhance organic matter content, improve soil structure, and increase carbon sequestration. Both varieties of green manures enhance soil biodiversity by promoting microbial activity and nurturing beneficial microbial populations, such as nitrogen-fixing bacteria and mycorrhizal fungi. Furthermore, they recycle nutrients, inhibit soil-borne diseases, and enhance nutrient availability, promoting a nutrient-dense and biologically active soil ecosystem. The cumulative impacts lead to increased soil fertility, greater resilience against erosion and climate stress, and extended productivity of agroecosystems.</p>
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<p>An overview of intercropping systems highlights their beneficial impacts on cash crops and long-term agroecosystem sustainability. Intercropping improves nutrient use efficiency, enhances soil health, promotes biodiversity, reduces pest and disease incidence, and increases crop resilience, contributing to stable yields and sustainable agricultural systems.</p>
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<p>We present a conceptual depiction of the integration of crop rotation systems, tracing their journey from challenges to outcomes. Crop rotation systems that incorporate cash and legume crops can sustain agricultural yields, increase farmers’ income, and minimize greenhouse gas emissions through legumes’ biological nitrogen fixation, which partially replaces synthetic nitrogen inputs. Incorporating legumes into crop rotations can improve soil health by promoting microbial and enzyme activity, optimizing nutrient cycling, and enhancing carbon sequestration.</p>
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19 pages, 663 KiB  
Review
Optimal Pathways to Lung Cancer Screening in Primary Care Settings: A Scoping Review
by Emmanouil K. Symvoulakis, Izolde Bouloukaki, Antonios Christodoulakis, Antonia Aravantinou-Karlatou and Ioanna Tsiligianni
Curr. Oncol. 2025, 32(1), 8; https://doi.org/10.3390/curroncol32010008 - 26 Dec 2024
Viewed by 377
Abstract
Lung cancer is the leading cause of cancer-related deaths worldwide, and delayed detection contributes to poor outcomes. Primary care plays a crucial role in early diagnosis, but detecting lung cancer early remains challenging for general practitioners (GPs). Therefore, the aim of this scoping [...] Read more.
Lung cancer is the leading cause of cancer-related deaths worldwide, and delayed detection contributes to poor outcomes. Primary care plays a crucial role in early diagnosis, but detecting lung cancer early remains challenging for general practitioners (GPs). Therefore, the aim of this scoping review was to identify optimal strategies and pathways for lung cancer screening (LCS) in primary care settings globally. We conducted a scoping review by searching PubMed, Scopus, and the Cochrane Library for relevant studies published in the past 10 years. Our keywords included “lung cancer”, “primary care”, “early detection”, “screening”, “best practices”, and “pathways”. We included randomized controlled trials, cross-sectional studies, and cohort studies focused on lung cancer screening in primary care. We extracted data on study characteristics, screening pathways, and key findings. We identified 18 studies that met our inclusion criteria. Important strategies for LCS included the use of shared decision-making tools, electronic health record (HER) prompts, risk prediction models, community outreach, and integration with smoking cessation programs. Barriers to implementation included the lack of provider familiarity with guidelines, time constraints, and patient factors. Healthcare professionals and policy makers in primary care settings can leverage this information to integrate the most effective screening strategies into their care, thus enhancing early detection rates and subsequently reducing global lung cancer morbidity and mortality. Full article
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<p>PRISMA flow diagram for this scoping review.</p>
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21 pages, 5177 KiB  
Article
The Representational Challenge of Integration and Interoperability in Transformed Health Ecosystems
by Bernd Blobel, Frank Oemig, Pekka Ruotsalainen, Mathias Brochhausen, Kevin W. Sexton and Mauro Giacomini
J. Pers. Med. 2025, 15(1), 4; https://doi.org/10.3390/jpm15010004 - 25 Dec 2024
Viewed by 186
Abstract
Background/Objectives: Health and social care systems around the globe are currently undergoing a transformation towards personalized, preventive, predictive, participative precision medicine (5PM), considering the individual health status, conditions, genetic and genomic dispositions, etc., in personal, social, occupational, environmental, and behavioral contexts. This [...] Read more.
Background/Objectives: Health and social care systems around the globe are currently undergoing a transformation towards personalized, preventive, predictive, participative precision medicine (5PM), considering the individual health status, conditions, genetic and genomic dispositions, etc., in personal, social, occupational, environmental, and behavioral contexts. This transformation is strongly supported by technologies such as micro- and nanotechnologies, advanced computing, artificial intelligence, edge computing, etc. Methods: To enable communication and cooperation between actors from different domains using different methodologies, languages, and ontologies based on different education, experiences, etc., we have to understand the transformed health ecosystem and all its components in terms of structure, function and relationships in the necessary detail, ranging from elementary particles up to the universe. In this way, we advance design and management of the complex and highly dynamic ecosystem from data to knowledge level. The challenge is the consistent, correct, and formalized representation of the transformed health ecosystem from the perspectives of all domains involved, representing and managing them based on related ontologies. The resulting business viewpoint of the real-world ecosystem must be interrelated using the ISO/IEC 21838 Top Level Ontologies standard. Thereafter, the outcome can be transformed into implementable solutions using the ISO/IEC 10746 Open Distributed Processing Reference Model. Results: The model and framework for this system-oriented, architecture-centric, ontology-based, policy-driven approach have been developed by the first author and meanwhile standardized as ISO 23903 Interoperability and Integration Reference Architecture. The formal representation of any ecosystem and its development process including examples of practical deployment of the approach, are presented in detail. This includes correct systems and standards integration and interoperability solutions. A special issue newly addressed in the paper is the correct and consistent formal representation Conclusions: of all components in the development process, enabling interoperability between and integration of any existing representational artifacts such as models, work products, as well as used terminologies and ontologies. The provided solution is meanwhile mandatory at ISOTC215, CEN/TC251 and many other standards developing organization in health informatics for all projects covering more than just one domain. Full article
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<p>Generic Component Model reference architecture.</p>
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<p>Generic reference architecture.</p>
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<p>Chomsky Hierarchy [<a href="#B16-jpm-15-00004" class="html-bibr">16</a>].</p>
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<p>Types of ontologies (from [<a href="#B15-jpm-15-00004" class="html-bibr">15</a>], changed).</p>
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<p>BFO 2020 as a Hierarchy (from [<a href="#B17-jpm-15-00004" class="html-bibr">17</a>]).</p>
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<p>ISO 23903 Framework in the light of good modeling best practices.</p>
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<p>OBO Foundry Ontologies (from [<a href="#B18-jpm-15-00004" class="html-bibr">18</a>]).</p>
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<p>Mapping of HL7 v.2 and HL7 v.3 [<a href="#B7-jpm-15-00004" class="html-bibr">7</a>].</p>
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<p>Mapping of ISO 12967 HISA and ISO 13940 ContSys [<a href="#B7-jpm-15-00004" class="html-bibr">7</a>].</p>
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<p>Integration of existing components and representational artifacts.</p>
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<p>Examples of reusable models.</p>
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<p>Example model with updates (simplified excerpt from MI-I [<a href="#B24-jpm-15-00004" class="html-bibr">24</a>]). * stands for “many” in the sense maxi-cardinality.</p>
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<p>Representation of business view integrating multiple domains. Black squares represent individuals; boxes represent ontology classes; dotted lines link individuals to their classes; and arrows represent relationships between individuals.</p>
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<p>HTS V1 system architecture.</p>
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<p>HTS V2 architecture.</p>
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19 pages, 17708 KiB  
Article
A Comparative Analysis of Explainable Artificial Intelligence Models for Electric Field Strength Prediction over Eight European Cities
by Yiannis Kiouvrekis, Ioannis Givisis, Theodor Panagiotakopoulos, Ioannis Tsilikas, Agapi Ploussi, Ellas Spyratou and Efstathios P. Efstathopoulos
Sensors 2025, 25(1), 53; https://doi.org/10.3390/s25010053 - 25 Dec 2024
Viewed by 229
Abstract
The widespread propagation of wireless communication devices, from smartphones and tablets to Internet of Things (IoT) systems, has become an integral part of modern life. However, the expansion of wireless technology has also raised public concern about the potential health risks associated with [...] Read more.
The widespread propagation of wireless communication devices, from smartphones and tablets to Internet of Things (IoT) systems, has become an integral part of modern life. However, the expansion of wireless technology has also raised public concern about the potential health risks associated with prolonged exposure to electromagnetic fields. Our objective is to determine the optimal machine learning model for constructing electric field strength maps across urban areas, enhancing the field of environmental monitoring with the aid of sensor-based data collection. Our machine learning models consist of a novel and comprehensive dataset collected from a network of strategically placed sensors, capturing not only electromagnetic field readings but also additional urban features, including population density, levels of urbanization, and specific building characteristics. This sensor-driven approach, coupled with explainable AI, enables us to identify key factors influencing electromagnetic exposure more accurately. The integration of IoT sensor data with machine learning opens the potential for creating highly detailed and dynamic electromagnetic pollution maps. These maps are not merely static snapshots; they offer researchers the ability to track trends over time, assess the effectiveness of mitigation efforts, and gain a deeper understanding of electromagnetic field distribution in urban environments. Through the extensive dataset, our models can yield highly accurate and dynamic electric field strength maps. For this study, we performed a comprehensive analysis involving 566 machine learning models across eight French cities: Lyon, Saint-Étienne, Clermont-Ferrand, Dijon, Nantes, Rouen, Lille, and Paris. The analysis incorporated six core approaches: k-Nearest Neighbors, XGBoost, Random Forest, Neural Networks, Decision Trees, and Linear Regression. The findings underscore the superior predictive capabilities of ensemble methods such as Random Forests and XGBoost, which outperform individual models. Simpler approaches like Decision Trees and k-NN offer effective yet slightly less precise alternatives. Neural Networks, despite their complexity, highlight the potential for further refinement in this application. In addition, our results show that the machine learning models significantly outperform the linear regression baseline, demonstrating the added value of more complex techniques in this domain. Our SHAP analysis reveals that the feature importance rankings in tree-based machine learning models differ significantly from those in k-NN, neural network, and linear regression models. Full article
(This article belongs to the Special Issue Artificial Intelligence and Sensors Technology in Smart Cities)
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<p>The distribution of the measurement points over France.</p>
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<p>The distribution of the measurement points over the cities.</p>
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<p>The methodology’s flowchart.</p>
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<p>SHAP Summary plot for each machine learning method. (<b>a</b>) SHAP summary plot for random forest models. (<b>b</b>) SHAP summary plot for XGBoost models. (<b>c</b>) SHAP summary plot for k-NN models. (<b>d</b>) SHAP summary plot for decision tree models.(<b>e</b>) SHAP summary plot for neural network models. (<b>f</b>) SHAP summary plot for linear regression models.</p>
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17 pages, 2979 KiB  
Article
Effects of Lactiplantibacillus plantarum and Fermentation Time on the Quality, Bacterial Community, and Functional Prediction of Silage from Lotus corniculatus L. in Karst Regions
by Yang Wang, Yang Yang, Xiaoyu Yang, Lei Huang, Puchang Wang and Lili Zhao
Agriculture 2025, 15(1), 16; https://doi.org/10.3390/agriculture15010016 - 25 Dec 2024
Viewed by 165
Abstract
Abstract: To improve the silage quality of Lotus corniculatus L. and expedite the promotion of cultivated varieties, this study investigates the impact of Lactiplantibacillus plantarum on the fermentation characteristics, bacterial community, and functional aspects of silage. The experiment included a control (CK) and [...] Read more.
Abstract: To improve the silage quality of Lotus corniculatus L. and expedite the promotion of cultivated varieties, this study investigates the impact of Lactiplantibacillus plantarum on the fermentation characteristics, bacterial community, and functional aspects of silage. The experiment included a control (CK) and a Lactiplantibacillus plantarum (LP) treatment, with sampling conducted at 3, 7, 15, and 45 days of fermentation to monitor nutritional value and fermentation quality, as well as changes in the bacterial community at 3 and 45 days. The results indicated that compared to the CK, the addition of LP significantly increased the lactic acid, dry matter, and crude protein content (p < 0.05) while substantially decreasing the water-soluble carbohydrates, pH, NH3-N, and acetic acid levels (p < 0.05). And the effect of adding LP was the most significant after 45 days of fermentation. LP promoted the growth of beneficial bacteria and inhibited harmful bacteria, with LP becoming the predominant genus and species after 45 days of fermentation. The metabolic pathway analysis revealed that the addition of LP enhanced carbohydrate metabolism and improved the replication and repair, translation, transcription, and membrane transport functions of the bacterial community. In summary, the addition of LP significantly enhances the silage quality of L. corniculatus and may serve as an effective method for promoting the application of L. corniculatus in karst regions. Full article
(This article belongs to the Special Issue Current Challenges in Microbiology and Chemistry of Animal Feed)
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<p>Principal coordinate analysis (PCOA) of bacterial communities in fresh and silaged <span class="html-italic">Lotus corniculatus</span>. FM represents the fresh <span class="html-italic">Lotus corniculatus</span>; CK3 represents the control silage on day 3; LP3 represents the silage on day 3 with added LP; CK45 represents the control silage on day 45; and LP45 signifies the silage on day 45 with added LP.</p>
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<p>Relative abundance of bacterial genera (<b>A</b>) and species (<b>B</b>) in fresh and silaged <span class="html-italic">Lotus corniculatus</span> after 3 and 45 days of fermentation. FM represents the fresh <span class="html-italic">Lotus corniculatus</span>; CK3 represents the control silage on day 3; LP3 represents the silage on day 3 with added LP; CK45 represents the control silage on day 45; and LP45 signifies the silage on day 45 with added LP.</p>
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<p>Comparison of microbial variation in fresh <span class="html-italic">Lotus corniculatus</span> samples (<b>A</b>) and silage after 3 days and 45 days (<b>B</b>) using the LEfSe online tool. This analysis identifies indicator microorganisms in the silage community with linear discriminant analysis (LDA) scores of 4 or higher under different treatments. FM represents the fresh <span class="html-italic">Lotus corniculatus</span>; CK3 represents the control silage on day 3; LP3 represents the silage on day 3 with added LP; CK45 represents the control silage on day 45; and LP45 signifies the silage on day 45 with added LP.</p>
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<p>Heatmap showing the correlation between silage fermentation quality and the relative abundance of bacterial species. DM represents dry matter, NDF represents neutral detergent fiber, ADF represents acid detergent fiber, WSC represents water-soluble carbohydrate, NT represents ammonia nitrogen, LA represents lactic acid, AA represents acetic acid, and CP represents crude protein. * <span class="html-italic">p</span> &lt; 0.05; ** <span class="html-italic">p</span> &lt; 0.01.</p>
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<p>Prediction of potential functions of bacterial communities in fresh <span class="html-italic">Lotus corniculatus</span> samples and silage after 3 and 45 days fermentation using PICRUSt. (<b>A</b>) The predicted first-level KEGG pathways; (<b>B</b>–<b>D</b>) the predicted second-level KEGG pathways. (<b>E</b>) Tshe heatmap of relative abundances for the predicted third-level KEGG pathways. ** Indicates a significant difference. (<span class="html-italic">p</span> &lt; 0.05).</p>
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17 pages, 1782 KiB  
Article
The Effects of Mixed Inoculum Storage Time on In Vitro Rumen Fermentation Characteristics, Microbial Diversity, and Community Composition
by Chang Liu, Jing Ge, Jiaqi Dai, Mingren Qu, Kehui Ouyang and Qinghua Qiu
Animals 2025, 15(1), 5; https://doi.org/10.3390/ani15010005 - 24 Dec 2024
Viewed by 179
Abstract
This study aimed to investigate the effects of different storage times of the mixed inoculum on in vitro rumen fermentation characteristics, microbial diversity, and community composition. The experiment was divided into five groups, with mixed inoculum composed of fresh rumen fluid and culture [...] Read more.
This study aimed to investigate the effects of different storage times of the mixed inoculum on in vitro rumen fermentation characteristics, microbial diversity, and community composition. The experiment was divided into five groups, with mixed inoculum composed of fresh rumen fluid and culture medium being stored at 39 °C for 0 h (H0), 12 h (H12), 24 h (H24), 36 h (H36), and 48 h (H48). After 48 h of in vitro fermentation, the fermentation fluid was collected to assess rumen fermentation characteristics and microbial community composition. The H24 group showed higher total gas production, ammoniacal nitrogen levels, and total volatile fatty acids, as well as higher concentrations of individual volatile fatty acids except propionate, compared to the H0 and H48 groups (p < 0.05). The Shannon and Simpson evenness indices were significantly higher in the H0, H12, and H24 groups than in the H48 group (p < 0.05). A total of nine phyla and sixteen genera involved in starch and fiber degradation were found to be more abundant in the H24 or H48 groups (p < 0.05). Moreover, nine predicted metabolic pathways were observed to be significantly enriched in either the H24 or H48 group (p < 0.05). Both principal coordinates analysis (PCoA) and non-metric multidimensional scaling (NMDS) analysis revealed distinct clustering patterns among the H0, H12, H24, H36, and H48 groups, and analysis of similarities (ANOSIM) confirmed these significant differences (R = 1.00, p < 0.05). This study demonstrates that the storage time of mixed inoculum influences rumen fermentation characteristics and microbial community composition in a time-dependent manner. It is recommended to use a mixed inoculum that has been stored within 24 h in an anaerobic environment at 39 °C for in vitro rumen fermentation tests. This study offers valuable microbial insights into the storage strategies for mixed inoculum, thereby improving the methodologies for variable control in in vitro rumen fermentation techniques. Full article
(This article belongs to the Section Animal Nutrition)
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<p>Gas production dynamics in response to incubation time across various mixed inoculum storage times. H0, H12, H24, H36, and H48 indicate the mixed inoculum used in the in vitro fermentation tests was stored for 0 h, 12 h, 24 h, 36 h, and 48 h, respectively.</p>
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<p>Rumen bacterial beta diversity across different storage times of mixed inoculum. (<b>a</b>) Principal coordinates analysis (PCoA); (<b>b</b>) non-metric multidimensional scaling (NMDS). H0, H12, H24, H36, and H48 indicate the mixed inoculum used in the in vitro fermentation tests was stored for 0 h, 12 h, 24 h, 36 h, and 48 h, respectively.</p>
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<p>Illustration of the effects of mixed inoculum storage times on discriminative bacterial communities at multiple taxonomic levels: (<b>a</b>) linear discriminant analysis; and (<b>b</b>) cladogram. H0, H12, H24, H36, and H48 indicate the mixed inoculum used in the in vitro fermentation tests was stored for 0 h, 12 h, 24 h, 36 h, and 48 h, respectively.</p>
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18 pages, 1057 KiB  
Article
The Predictability of Stress Coping Strategies and Psychological Capital on the Psychological Well-Being of Autistic Spectrum Children’s Mothers in the Kingdom of Saudi Arabia
by Nawal A. Al Eid, Sami M. Alshehri and Boshra A. Arnout
Behav. Sci. 2024, 14(12), 1235; https://doi.org/10.3390/bs14121235 - 23 Dec 2024
Viewed by 469
Abstract
There is an increasing number of studies concerned with the study of children with autism spectrum disorder (ASD). At the same time, there is a lack of interest in studies on their families, especially on mothers who represent the first breadwinner for a [...] Read more.
There is an increasing number of studies concerned with the study of children with autism spectrum disorder (ASD). At the same time, there is a lack of interest in studies on their families, especially on mothers who represent the first breadwinner for a child who suffers from a deficit in social communication with others, reflected in their well-being (PWB). This study aimed to reveal the possibility of predicting the PWB of autistic spectrum children’s mothers through the variables of coping strategies and psychological capital (PsyCap). The study used a predictive, descriptive research method to reveal the ability of the variables—coping strategies (problem-solving, avoidance, support, re-evaluation, remorse) and PsyCap (self-efficacy, optimism, hope, and resilience)—in predicting the PWB of autistic spectrum children’s mothers. The study sample consisted of (248) mothers, to whom coping strategies, PsyCap, and PWB measures were applied. The results showed that there were statistically significant differences between working and housewife mothers of autistic spectrum children in solving problems (t = 3.162, p < 0.002), avoidance (t = 1.973, p < 0.05), positive coping (t = 2.307, p < 0.022), self-efficacy (t = 3.667, p < 0.000), resilience (t = 3.338, p < 0.001), PsyCap (t = 2.866, p < 0.005), and PWB (t = 2.549, p < 0.011). Meanwhile, there were no statistically significant differences in social support, problem reassessment, remorse, negative coping, optimism, and hope. Also, there were no statistically significant differences due to the number of children in coping strategies, PsyCap, and PWB. The results also showed that there were statistically significant differences at the level of significance (0.05) between mothers whose age was less than 40 years and those 40 years and older in solving problems (t = 2.093, p < 0.037) in favor of mothers of the age group 40 years and older (M = 22.00, SD = 1.22), and avoidance (t = 1.987, p < 0.048) in favor of mothers under 40 years of age (M = 6.228, SD = 0.464). However, there were no statistically significant differences in social support, problem reassessment, remorse, positive coping, negative coping, self-efficacy, optimism, hope, resilience, the total degree of PsyCap, and well-being due to the variable of the mother’s age. The regression analysis results showed that optimism and problem-solving contributed to (39.90%) of the total change in PWB for mothers of children with autism spectrum. The study’s findings indicate the need to develop the ability of autistic spectrum children’s mothers to solve problems and their PsyCap, which is represented in self-efficacy, optimism, hope, and resilience, to enhance their PWB, which may have a positive impact on their autistic spectrum child. Full article
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<p>Differences between employed and not employed autistic spectrum children in coping strategies, PsyCap, and PWB.</p>
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<p>Differences between mothers of autistic spectrum children in coping strategies, PsyCap, and PWB due to the number of children.</p>
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<p>Differences between mothers of autistic spectrum children in coping strategies, PsyCap, and PWB due to the mother’s age.</p>
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31 pages, 1953 KiB  
Article
UAV Trajectory Control and Power Optimization for Low-Latency C-V2X Communications in a Federated Learning Environment
by Xavier Fernando and Abhishek Gupta
Sensors 2024, 24(24), 8186; https://doi.org/10.3390/s24248186 - 22 Dec 2024
Viewed by 785
Abstract
Unmanned aerial vehicle (UAV)-enabled vehicular communications in the sixth generation (6G) are characterized by line-of-sight (LoS) and dynamically varying channel conditions. However, the presence of obstacles in the LoS path leads to shadowed fading environments. In UAV-assisted cellular vehicle-to-everything (C-V2X) communication, vehicle and [...] Read more.
Unmanned aerial vehicle (UAV)-enabled vehicular communications in the sixth generation (6G) are characterized by line-of-sight (LoS) and dynamically varying channel conditions. However, the presence of obstacles in the LoS path leads to shadowed fading environments. In UAV-assisted cellular vehicle-to-everything (C-V2X) communication, vehicle and UAV mobility and shadowing adversely impact latency and throughput. Moreover, 6G vehicular communications comprise data-intensive applications such as augmented reality, mixed reality, virtual reality, intelligent transportation, and autonomous vehicles. Since vehicles’ sensors generate immense amount of data, the latency in processing these applications also increases, particularly when the data are not independently identically distributed (non-i.i.d.). Furthermore, when the sensors’ data are heterogeneous in size and distribution, the incoming packets demand substantial computing resources, energy efficiency at the UAV servers and intelligent mechanisms to queue the incoming packets. Due to the limited battery power and coverage range of UAV, the quality of service (QoS) requirements such as coverage rate, UAV flying time, and fairness of vehicle selection are adversely impacted. Controlling the UAV trajectory so that it serves a maximum number of vehicles while maximizing battery power usage is a potential solution to enhance QoS. This paper investigates the system performance and communication disruption between vehicles and UAV due to Doppler effect in the orthogonal time–frequency space (OTFS) modulated channel. Moreover, a low-complexity UAV trajectory prediction and vehicle selection method is proposed using federated learning, which exploits related information from past trajectories. The weighted total energy consumption of a UAV is minimized by jointly optimizing the transmission window (Lw), transmit power and UAV trajectory considering Doppler spread. The simulation results reveal that the weighted total energy consumption of the OTFS-based system decreases up to 10% when combined with federated learning to locally process the sensor data at the vehicles and communicate the processed local models to the UAV. The weighted total energy consumption of the proposed federated learning algorithm decreases by 10–15% compared with convex optimization, heuristic, and meta-heuristic algorithms. Full article
(This article belongs to the Section Communications)
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<p>A brief timeline depicting the amalgamation of wireless communication technologies with transportation systems. Also illustrated is the gradual integration of UAVs in vehicular networks in 5G and 6G wireless communication paradigms. A detailed timeline and comprehensive overview of the recent and evolving applications of machine learning techniques in UAV communication frameworks can be found in [<a href="#B14-sensors-24-08186" class="html-bibr">14</a>,<a href="#B15-sensors-24-08186" class="html-bibr">15</a>].</p>
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<p>System Model: Delay is accumulated as vehicles in different clusters generate and transmit local models to the UAV. The UAV transmits the global model to the vehicles. Note, each vehicle captures a different kind of data packet, leading to non-i.i.d. and heterogeneous data.</p>
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<p>An illustration of the proposed federated reinforcement learning-based solution approach for UAV trajectory control and power optimization for low-latency C-V2X communications.</p>
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<p>UAV trajectory varies in a random manner, and the vehicles capture varying sensor data at different TTIs. By processing the sensor data, local models are generated at the vehicles and a global model is generated at the UAV.</p>
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<p>UAV trajectory and vehicle coverage depending on UAV transmit power (<math display="inline"><semantics> <mrow> <msub> <mi>P</mi> <mi>i</mi> </msub> <mrow> <mo>(</mo> <mi>t</mi> <mo>)</mo> </mrow> </mrow> </semantics></math>) and altitude (<math display="inline"><semantics> <mi mathvariant="script">H</mi> </semantics></math>). The shaded triangular region (<math display="inline"><semantics> <mrow> <msub> <mi>P</mi> <mi>i</mi> </msub> <mrow> <mo>(</mo> <mi>t</mi> <mo>)</mo> </mrow> </mrow> </semantics></math>) indicates the coverage range of the UAV when the UAV is at a specific altitude (<math display="inline"><semantics> <mi mathvariant="script">H</mi> </semantics></math>).</p>
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<p>Variation in average cost function (UAV energy and latency) with number of vehicles (<span class="html-italic">V</span>).</p>
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<p>Variation in queuing delay (<math display="inline"><semantics> <msub> <mi>D</mi> <mrow> <mi>q</mi> <mi>u</mi> <mi>e</mi> </mrow> </msub> </semantics></math>) in FL scenario with time slots.</p>
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<p>Total delay (<math display="inline"><semantics> <mi mathvariant="bold-script">D</mi> </semantics></math>) vs. number of vehicles (<span class="html-italic">V</span>) for different machine learning models.</p>
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<p>Variation in average packet drop rate with control parameter (<math display="inline"><semantics> <mi>ϱ</mi> </semantics></math>) using fed-DDPG.</p>
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<p>Variation in average UAV energy with number of vehicles (<span class="html-italic">V</span>) for different machine learning models.</p>
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<p>Variation in FL computation rate (Mbits/s) with control parameter (<math display="inline"><semantics> <mi>ϱ</mi> </semantics></math>) for different machine learning models.</p>
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<p>Probability of optimal trajectory prediction for fed-DDPG (using LSTM) vs. UAV altitude (<math display="inline"><semantics> <mi mathvariant="script">H</mi> </semantics></math>) for varying number of vehicles (<span class="html-italic">V</span>) over trials of 250 episodes.</p>
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<p>Probability of optimal trajectory prediction for actor–critic (using LSTM) vs. UAV altitude (<math display="inline"><semantics> <mi mathvariant="script">H</mi> </semantics></math>) for varying number of vehicles (<span class="html-italic">V</span>) over trials of 500 episodes.</p>
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<p>Probability of optimal trajectory prediction for CNN-LSTM vs. UAV altitude (<math display="inline"><semantics> <mi mathvariant="script">H</mi> </semantics></math>) for varying number of vehicles (<span class="html-italic">V</span>) over trials of 1000 episodes.</p>
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<p>Probability of optimal trajectory prediction for RNN vs. UAV altitude (<math display="inline"><semantics> <mi mathvariant="script">H</mi> </semantics></math>) for varying number of vehicles (<span class="html-italic">V</span>) over trials of 1000 episodes.</p>
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<p>Probability of optimal trajectory prediction for GRU vs. UAV altitude (<math display="inline"><semantics> <mi mathvariant="script">H</mi> </semantics></math>) for varying number of vehicles (<span class="html-italic">V</span>) over trials of 1000 episodes.</p>
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<p>UAV transmit power (<math display="inline"><semantics> <mrow> <msub> <mi>P</mi> <mi>i</mi> </msub> <mrow> <mo>(</mo> <mi>t</mi> <mo>)</mo> </mrow> </mrow> </semantics></math>) vs. SNR in OTFS modulation scheme for varying number of vehicles (<span class="html-italic">V</span>).</p>
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15 pages, 3582 KiB  
Article
Enhancing IoT-Based Environmental Monitoring and Power Forecasting: A Comparative Analysis of AI Models for Real-Time Applications
by Md Minhazur Rahman, Md Ibne Joha, Md Shahriar Nazim and Yeong Min Jang
Appl. Sci. 2024, 14(24), 11970; https://doi.org/10.3390/app142411970 - 20 Dec 2024
Viewed by 424
Abstract
The Internet of Things (IoT) is transforming industries by integrating sensors and connectivity into everyday objects, enabling enhanced monitoring, management, and automation through Machine-to-Machine (M2M) communication. Despite these advancements, the IoT faces limitations in accurately predicting environmental conditions and power consumption. This study [...] Read more.
The Internet of Things (IoT) is transforming industries by integrating sensors and connectivity into everyday objects, enabling enhanced monitoring, management, and automation through Machine-to-Machine (M2M) communication. Despite these advancements, the IoT faces limitations in accurately predicting environmental conditions and power consumption. This study proposes an advanced IoT platform that combines real-time data collection with secure transmission and forecasting using a hybrid Long Short-Term Memory (LSTM)–Gated Recurrent Unit (GRU) model. The hybrid architecture addresses the computational inefficiencies of LSTM and the short-term dependency challenges of GRU, achieving improved accuracy and efficiency in time-series forecasting. For all prediction use cases, the model achieves a Maximum Mean Absolute Error (MAE) of 3.78%, Root Mean Square Error (RMSE) of 8.15%, and a minimum R2 score of 82.04%, the showing proposed model’s superiority for real-life use cases. Furthermore, a comparative analysis also shows the performance of the proposed model outperforms standalone LSTM and GRU models, enhancing the IoT’s reliability in real-time environmental and power forecasting. Full article
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<p>Data collection system for power forecasting.</p>
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<p>Block diagram of data processing and AI model training.</p>
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<p>Architecture of LSTM model.</p>
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<p>Structure of GRU model.</p>
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<p>Proposed architecture of LSTM-GRU hybrid model.</p>
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<p>Comparison of actual and predicted value for (<b>a</b>) air conditioner and (<b>b</b>) water dispenser.</p>
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<p>Comparison of actual and predicted value for refrigerator.</p>
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<p>Comparison of actual and predicted value for (<b>a</b>) temperature and (<b>b</b>) humidity.</p>
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<p>Comparison of actual and predicted values for (<b>a</b>) AC, (<b>b</b>) refrigerator, (<b>c</b>) water dispenser, (<b>d</b>) temperature, and (<b>e</b>) humidity.</p>
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18 pages, 5346 KiB  
Article
Metagenome Analysis Identified Novel Microbial Diversity of Sandy Soils Surrounded by Natural Lakes and Artificial Water Points in King Salman Bin Abdulaziz Royal Natural Reserve, Saudi Arabia
by Yahya S. Al-Awthan, Rashid Mir, Fuad A. Alatawi, Abdulaziz S. Alatawi, Fahad M. Almutairi, Tamer Khafaga, Wael M. Shohdi, Amal M. Fakhry and Basmah M. Alharbi
Life 2024, 14(12), 1692; https://doi.org/10.3390/life14121692 - 20 Dec 2024
Viewed by 468
Abstract
Background: Soil microbes play a vital role in the ecosystem as they are able to carry out a number of vital tasks. Additionally, metagenomic studies offer valuable insights into the composition and functional potential of soil microbial communities. Furthermore, analyzing the obtained data [...] Read more.
Background: Soil microbes play a vital role in the ecosystem as they are able to carry out a number of vital tasks. Additionally, metagenomic studies offer valuable insights into the composition and functional potential of soil microbial communities. Furthermore, analyzing the obtained data can improve agricultural restoration practices and aid in developing more effective environmental management strategies. Methodology: In November 2023, sandy soil samples were collected from ten sites of different geographical areas surrounding natural lakes and artificial water points in the Tubaiq conservation area of King Salman Bin Abdulaziz Royal Natural Reserve (KSRNR), Saudi Arabia. In addition, genomic DNA was extracted from the collected soil samples, and 16S rRNA sequencing was conducted using high-throughput Illumina technology. Several computational analysis tools were used for gene prediction and taxonomic classification of the microbial groups. Results: In this study, sandy soil samples from the surroundings of natural and artificial water resources of two distinct natures were used. Based on 16S rRNA sequencing, a total of 24,563 OTUs were detected. The metagenomic information was then categorized into 446 orders, 1036 families, 4102 genera, 213 classes, and 181 phyla. Moreover, the phylum Pseudomonadota was the most dominant microbial community across all samples, representing an average relative abundance of 34%. In addition, Actinomycetes was the most abundant class (26%). The analysis of clustered proteins assigned to COG categories provides a detailed understanding of the functional capabilities and adaptation of microbial communities in soil samples. Amino acid metabolism and transport were the most abundant categories in the soil environment. Conclusions: Metagenome analysis of sandy soils surrounding natural lakes and artificial water points in the Tubaiq conservation area of KSRNR (Saudi Arabia) has unveils rich microbial activity, highlighting the complex interactions and ecological roles of microbial communities in these environments. Full article
(This article belongs to the Special Issue Trends in Microbiology 2025)
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<p>(<b>A</b>) A map showing research area inside within Al-Tubaiq region of the KSRNR. (<b>B</b>) Locations of the sampling sites within the Tubaiq area of KSRNR.</p>
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<p>(<b>A</b>) A map showing research area inside within Al-Tubaiq region of the KSRNR. (<b>B</b>) Locations of the sampling sites within the Tubaiq area of KSRNR.</p>
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<p>Proportional abundance of key microbial orders and phyla in samples of soil. (<b>A</b>) Relative abundance of bacteria based on phylum. (<b>B</b>) Relative abundance of bacteria based on order.</p>
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<p>Proportional abundance of key microbial families and classes in samples of soil. (<b>A</b>) Relative abundance of bacteria based on class. (<b>B</b>) Relative abundance of bacteria based on family.</p>
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<p>Proportional abundance of key microbial species and genera in samples of soil. (<b>A</b>) Relative abundance of bacteria based on genus. (<b>B</b>) Relative abundance of bacteria based on species.</p>
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<p>(<b>A</b>) Correlation matrix illustrating the relationships between soil samples based on bacterial species prevalence. * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001) (<b>B</b>) Heatmap and hierarchical clustering of soil samples and dominant species distribution.</p>
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<p>(<b>A</b>) Enriched OTUs by COG functional groups. (<b>B</b>) Average OTU counts per COG category.</p>
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