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Search Results (317)

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10 pages, 776 KiB  
Article
Effects of a Short-Term Soccer Training Intervention on Skill Course Performance in Youth Players: A Randomized Study
by Arne Sørensen, Terje Dalen and Pål Lagestad
Sports 2024, 12(12), 345; https://doi.org/10.3390/sports12120345 - 13 Dec 2024
Viewed by 465
Abstract
The aim of this study was to evaluate the effect of 11 additional soccer training sessions among youth soccer players according to their performance in a skill course. A total of 90 participants, aged 9 to 12, were randomly assigned to either an [...] Read more.
The aim of this study was to evaluate the effect of 11 additional soccer training sessions among youth soccer players according to their performance in a skill course. A total of 90 participants, aged 9 to 12, were randomly assigned to either an intervention group (IG) (n = 54) or a control group (CG) (n = 36) and have validated data. The trainings focused upon enhancing ball mastery and decision-making and included a combination of one vs. one situations and small-sided games (SSGs). Pre- and post-tests measured passing and dribbling skills through a skill course. The best time with additional time penalties for each dribbling and passing error was used for further analysis. An independent t-test revealed no significant differences in improvement between the two groups. However, paired t-tests revealed significant improvements for both the IG and the CG from pre- to post-test (7.9 and 3.9 s, respectively). Furthermore, no significant differences in the development of track time, cone touches, or passing errors between the groups were detected. These findings suggest that soccer players aged 9 to 12 improve their performance in a skill course through increased familiarity with the course and natural development of technical skills based on participation in soccer training and recreational soccer play. We argue that the lack of significant differences between the groups’ performances can be attributed to the short duration and few sessions of the intervention, and a somehow low similarity between the skill course and the activities in the sessions. Full article
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<p>Skill test course, the red, blue and yellow triangles is cones. The small blue squares are the area the players should stop the ball inside.</p>
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25 pages, 6353 KiB  
Article
Fractional-Order Controller for the Course Tracking of Underactuated Surface Vessels Based on Dynamic Neural Fuzzy Model
by Guangyu Li, Yanxin Li, Xiang Li, Mutong Liu, Xuesong Zhang and Hua Jin
Fractal Fract. 2024, 8(12), 720; https://doi.org/10.3390/fractalfract8120720 - 5 Dec 2024
Viewed by 472
Abstract
Aiming at the uncertainty problem caused by the time-varying modeling parameters associated with ship speed in the course tracking control of underactuated surface vessels (USVs), this paper proposes a control algorithm based on the dynamic neural fuzzy model (DNFM). The DNFM simultaneously adjusts [...] Read more.
Aiming at the uncertainty problem caused by the time-varying modeling parameters associated with ship speed in the course tracking control of underactuated surface vessels (USVs), this paper proposes a control algorithm based on the dynamic neural fuzzy model (DNFM). The DNFM simultaneously adjusts the structure and parameters during learning and fully approximates the inverse dynamics of ships. Online identification and modeling lays the model foundation for ship motion control. The trained DNFM, serving as an inverse controller, is connected in parallel with the fractional-order PIλDμ controller to be used for the tracking control of the ship’s course. Moreover, the weights of the model can be further adjusted during the course tracking. Taking the actual ship data of a 5446 TEU large container ship, simulation experiments are conducted, respectively, for course tracking, course tracking under wind and wave interferences, and comparison with five different controllers. This proposed controller can overcome the influence of the uncertainty of modeling parameters, tracking the desired course quickly and effectively. Full article
(This article belongs to the Special Issue Applications of Fractional-Order Systems to Automatic Control)
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<p>Motion coordinate system.</p>
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<p>Corresponding nonlinear ship model.</p>
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<p>Dynamic neural fuzzy model structure.</p>
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<p>Identification process of inverse model for ship course control.</p>
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<p>Flow of inverse model identification for ship course control based on DNFM.</p>
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<p>Ship course control system.</p>
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<p>Change in ship speed V.</p>
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<p>Change in ship model parameters <math display="inline"><semantics> <mrow> <mi mathvariant="normal">K</mi> </mrow> </semantics></math>, <math display="inline"><semantics> <mrow> <mi mathvariant="normal">T</mi> </mrow> </semantics></math>, <math display="inline"><semantics> <mrow> <mi mathvariant="sans-serif">α</mi> </mrow> </semantics></math>, and <math display="inline"><semantics> <mrow> <mi mathvariant="sans-serif">β</mi> </mrow> </semantics></math>.</p>
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<p>DNFM generating fuzzy rules.</p>
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<p>DNFM identification results.</p>
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<p>Root mean squared error in learning.</p>
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<p>DNFM identification error.</p>
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<p>Ship course tracking.</p>
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<p>Rudder control for ship course.</p>
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<p>Equivalent rudder angle of wind.</p>
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<p>Equivalent rudder angle of waves.</p>
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<p>DNFM generating fuzzy rules under wind and wave disturbances.</p>
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<p>DNFM identification results under wind and wave disturbances.</p>
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<p>Root mean squared error under wind and wave disturbances.</p>
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<p>Identification error of DNFM.</p>
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<p>Course control and rudder angle curves under wind and wave disturbances.</p>
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<p>Comparison of five different controllers.</p>
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<p>Rudder angle using five different controllers.</p>
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19 pages, 2075 KiB  
Review
Advancing Cardiac Amyloidosis Care Through Insights from Cardiopulmonary Exercise Testing
by Pietro Pugliatti, Giancarlo Trimarchi, Federico Barocelli, Fausto Pizzino, Francesco Di Spigno, Andrea Tedeschi, Maurizio Cusmà Piccione, Pierangela Irrera, Daniela Aschieri, Giampaolo Niccoli, Umberto Paradossi and Gianluca Di Bella
J. Clin. Med. 2024, 13(23), 7285; https://doi.org/10.3390/jcm13237285 - 29 Nov 2024
Viewed by 821
Abstract
Cardiac amyloidosis, encompassing both transthyretin (ATTR) and light-chain (AL) types, poses considerable challenges in patient management due to its intricate pathophysiology and progressive course. This narrative review elucidates the pivotal role of cardiopulmonary exercise testing (CPET) in the assessment of these patients. CPET [...] Read more.
Cardiac amyloidosis, encompassing both transthyretin (ATTR) and light-chain (AL) types, poses considerable challenges in patient management due to its intricate pathophysiology and progressive course. This narrative review elucidates the pivotal role of cardiopulmonary exercise testing (CPET) in the assessment of these patients. CPET is essential for evaluating disease progression by measuring cardio-respiratory performance and providing prognostic insights. This functional test is crucial not only for tracking the disease trajectory, but also for assessing the effectiveness of disease-modifying therapies. Moreover, CPET facilitates the customization of therapeutic strategies based on individual patient performance, enhancing personalized care. By objectively measuring parameters such as peak oxygen uptake (VO2 peak), ventilatory efficiency, and exercise capacity, clinicians can gain a deeper understanding of the degree of functional impairment and make informed decisions regarding treatment initiation, adjustment, and anticipated outcomes. This review emphasizes the importance of CPET in advancing personalized medicine approaches, ultimately striving to improve the quality of life and clinical outcomes for patients with cardiac amyloidosis. Full article
(This article belongs to the Special Issue Advances in Diagnosis and Treatment of Amyloidosis)
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Graphical abstract
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<p>Graphical abstract summarizing the role of the cardiopulmonary exercise test (CPET) in cardiac amyloidosis (CA).</p>
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<p>Wasserman Graphs. A 79-year-old male with transthyretin wild-type cardiac amyloidosis. Reduced peak VO<sub>2</sub> of 13.1 mL/kg/min, corresponding to 67% of the predicted value (<b>a</b>). Heart rate plateau at peak exercise, suggestive of chronotropic incompetence (<b>b</b>). Increased slope of VE/VCO<sub>2</sub> curve (<b>c</b>). Abbreviations: VO<sub>2</sub>—oxygen uptake, VCO<sub>2</sub>—carbon dioxide production, VE—pulmonary ventilation, HR—heart rate.</p>
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<p>Wasserman Graphs illustrating the improvement in CPET parameters in a 64-year-old male patient with wild-type cardiac amyloidosis before and 12 months after starting tafamidis therapy. Notable improvements include an increase in VO<sub>2</sub> peak (from 22 to 26) and a decrease in the slope of the VE/VCO<sub>2</sub> curve. Abbreviations: VO<sub>2</sub>—oxygen uptake, VCO<sub>2</sub>—carbon dioxide production, VE—pulmonary ventilation, HR—heart rate.</p>
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27 pages, 9669 KiB  
Article
Using LSTM with Trajectory Point Correlation and Temporal Pattern Attention for Ship Trajectory Prediction
by Yi Zhou, Haitao Guo, Jun Lu, Zhihui Gong, Donghang Yu and Lei Ding
Electronics 2024, 13(23), 4705; https://doi.org/10.3390/electronics13234705 - 28 Nov 2024
Viewed by 482
Abstract
Accurate ship trajectory prediction is crucial for real-time vessel position tracking and maritime safety management. However, existing methods for ship trajectory prediction encounter significant challenges. They struggle to effectively extract long-term and complex spatial–temporal features hidden within the data. Moreover, they often overlook [...] Read more.
Accurate ship trajectory prediction is crucial for real-time vessel position tracking and maritime safety management. However, existing methods for ship trajectory prediction encounter significant challenges. They struggle to effectively extract long-term and complex spatial–temporal features hidden within the data. Moreover, they often overlook correlations among multivariate dynamic features such as longitude (LON), latitude (LAT), speed over ground (SOG), and course over ground (COG), which are essential for precise trajectory forecasting. To address these pressing issues and fulfill the need for more accurate and comprehensive ship trajectory prediction, we propose a novel and integrated approach. Firstly, a Trajectory Point Correlation Attention (TPCA) mechanism is devised to establish spatial connections between trajectory points, thereby uncovering the local trends of trajectory point changes. Subsequently, a Temporal Pattern Attention (TPA) mechanism is introduced to handle the associations between multiple variables across different time steps and capture the dynamic feature correlations among trajectory attributes. Finally, a Great Circle Route Loss Function (GCRLoss) is constructed, leveraging the perception of the Earth’s curvature to deepen the understanding of spatial relationships and geographic information. Experimental results demonstrate that our proposed method outperforms existing ship trajectory prediction techniques, showing enhanced reliability in multi-step predictions. Full article
(This article belongs to the Special Issue AI-Driven Digital Image Processing: Latest Advances and Prospects)
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<p>The historical trajectory of a ship at sea in the first n moments predicts the trajectory at n + m moments.</p>
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<p>Trajectory prediction model framework.</p>
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<p>Trajectory Point Correlation Attention.</p>
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<p>LSTM network.</p>
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<p>Relationship chart of different ship attributes.</p>
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<p>Visualization of the relationships between different ship attributes.</p>
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<p>Four forms of erroneous and noisy impacts. (<b>a</b>) Abnormalities in the MMSI column; (<b>b</b>) irregularities in the COG column; (<b>c</b>) anomalies in the SOG column; (<b>d</b>) duplicate data.</p>
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<p>A map showing the area where the dataset was obtained.</p>
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<p>A comparison of the model results based on various indicators during the training process. (<b>a</b>) MAE of LAT. (<b>b</b>) MAE of LON. (<b>c</b>) RMSE of LAT. (<b>d</b>) RMSE of LON.</p>
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<p>Box plots of prediction MAE and RMSE with a length of 20 steps. (<b>a</b>) LAT prediction MAE. (<b>b</b>) LON prediction MAE. (<b>c</b>) LAT prediction RMSE. (<b>d</b>) LON prediction RMSE. Note: The circles inside the bars represent abnormal values, and the squares represent the mean values.</p>
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<p>Prediction results of the proposed model when sailing in a straight line.</p>
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<p>Prediction results of the proposed model for curved navigation with different SOG changes. (<b>a</b>) Prediction results with slow SOG changes. (<b>b</b>) Prediction results with rapid SOG changes.</p>
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<p>Comparison results of curved navigation trajectory prediction.</p>
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<p>Comparison of curved navigation prediction results. (<b>a</b>) Longitude prediction results; (<b>b</b>) latitude prediction results.</p>
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19 pages, 3575 KiB  
Article
Contributions to Incorporation of Non-Recyclable Plastics in Bituminous Mixtures
by João Fonseca, Vítor Antunes and Ana Cristina Freire
Sustainability 2024, 16(22), 9945; https://doi.org/10.3390/su16229945 - 14 Nov 2024
Viewed by 575
Abstract
Over the past 50 years, global plastic production has surged exponentially. Around 40% of this plastic is used for packaging, most of which is single-use, while 20% is used in construction. Despite the vast quantities produced, only about 6% of discarded plastics are [...] Read more.
Over the past 50 years, global plastic production has surged exponentially. Around 40% of this plastic is used for packaging, most of which is single-use, while 20% is used in construction. Despite the vast quantities produced, only about 6% of discarded plastics are properly recycled, 10% are incinerated, and the majority are disposed of without proper management. With low recycling rates and some plastics being non-recyclable or with limited recycling cycles, it is important to explore new ways of reusing this waste as secondary raw materials. This study explores the potential of incorporating non-recyclable plastic waste into bituminous mixtures. The objective is to develop a sustainable solution for surface courses with similar or better performance than traditional bituminous mixtures by incorporating plastic waste using the dry method. A bituminous mixture containing 10% non-recyclable plastic was formulated and tested for water sensitivity, wheel tracking, and stiffness modulus. Additionally, environmental and economic comparisons were performed with a standard surface mixture. Results showed increased water resistance, high resistance to permanent deformation, reduced stiffness, lower susceptibility to frequency and temperature variations, and greater flexibility. These findings suggest that adding plastic not only enhances mechanical properties but also reduces costs, offering a sustainable alternative for non-recyclable plastics in road construction. Full article
(This article belongs to the Section Sustainable Engineering and Science)
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<p>Plastic sample.</p>
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<p>Representative constituents: presentation and percentages.</p>
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<p>Grading curve.</p>
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<p>Melting point at 120 °C.</p>
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<p>(<b>a</b>) Porosity; (<b>b</b>) Marshall stability; (<b>c</b>) VMA; (<b>d</b>) deformation.</p>
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<p>(<b>a</b>) Porosity; (<b>b</b>) Marshall stability; (<b>c</b>) VMA; (<b>d</b>) deformation.</p>
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<p>Evaluation of water sensitivity: (<b>a</b>) indirect tensile strength in dry and wet conditions; (<b>b</b>) indirect tensile strength ratio.</p>
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<p>Permanent deformation.</p>
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<p>(<b>a</b>) Stiffness modulus; (<b>b</b>) phase angle; (<b>c</b>) storage modulus (E<sub>1</sub>); (<b>d</b>) loss modulus (E<sub>2</sub>).</p>
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<p>Cost analysis of bituminous mixtures components.</p>
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19 pages, 4383 KiB  
Article
Classification of Ship Type from Combination of HMM–DNN–CNN Models Based on Ship Trajectory Features
by Dae-Woon Shin and Chan-Su Yang
Remote Sens. 2024, 16(22), 4245; https://doi.org/10.3390/rs16224245 - 14 Nov 2024
Viewed by 457
Abstract
This study proposes an enhanced ship-type classification model that employs a sequential processing methodology integrating hidden Markov model (HMM), deep neural network (DNN), and convolutional neural network (CNN) techniques. Four different ship types—fishing boat, passenger, container, and other ship—were classified using multiple ship [...] Read more.
This study proposes an enhanced ship-type classification model that employs a sequential processing methodology integrating hidden Markov model (HMM), deep neural network (DNN), and convolutional neural network (CNN) techniques. Four different ship types—fishing boat, passenger, container, and other ship—were classified using multiple ship trajectory features extracted from the automatic identification system (AIS) and small fishing vessel tracking system. For model optimization, both ship datasets were transformed into various formats corresponding to multiple models, incorporating data enhancement and augmentation approaches. Speed over ground, course over ground, rate of turn, rate of turn in speed, berth distance, latitude/longitude, and heading were used as input parameters. The HMM–DNN–CNN combination was obtained as the optimal model (average F-1 score: 97.54%), achieving individual classification performances of 99.03%, 97.46%, and 95.83% for fishing boats, passenger ships, and container ships, respectively. The proposed approach outperformed previous approaches in prediction accuracy, with further improvements anticipated when implemented on a large-scale real-time data collection system. Full article
(This article belongs to the Special Issue Artificial Intelligence and Big Data for Oceanography)
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<p>Study area (red box) and trajectories of ships from 6 to 10 February 2021. The red dot indicates the location of the Korea Institute of Ocean Science and Technology, operating a monitoring station for merchant and fishing vessels. Blue and green lines depict the ship trajectories obtained from the AIS and V-Pass, respectively. Here, AIS = automatic identification system, and V-Pass = small fishing vessel tracking system.</p>
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<p>Trajectories of different ship types from the training dataset shown in <a href="#remotesensing-16-04245-t001" class="html-table">Table 1</a>. (<b>a</b>) Fishing boat, (<b>b</b>) passenger ship, (<b>c</b>) container ship, and (<b>d</b>) other ship.</p>
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<p>Overall workflow for ship type classification through combining of multiple models. Here, SOG = speed over ground, ROT = rate of turn, ROTS = rate of turn in speed, and COG = course over ground.</p>
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<p>Structure of hierarchical HMM model for classifying fishing boat. (<b>a</b>) The position-based probability of fishing activity was derived from two observational parameters, SOG and ROT, at each time step. (<b>b</b>) Fishing/non-fishing state estimated by the stochastic method based on SOG (<b>top</b>) and ROT (<b>bottom</b>).</p>
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<p>Flowchart for estimating the probability of the DNN model input values through filtering for passenger ship classification.</p>
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<p>Flowchart for estimating the probability of the CNN model input values by thresholding and filtering for container ship classification. Here, CP = container pier, PM = pier masking, and NCP = non-container pier.</p>
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<p>Case application of the HMM model and fishing boat trajectory feature analysis from the training dataset. (<b>a</b>) Labeling of classified trajectory into fishing (red circle) and non-fishing (blue circle). (<b>b</b>) Comparison of SOG and ROT distributions between fishing and non-fishing states.</p>
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<p>Analysis of passenger ship trajectory features from the training dataset. (<b>a</b>) Example of a passenger ship trajectory on 10 February 2021. (<b>b</b>) Comparative analysis between passenger and other ship types based on the probability of parameters: berth distance, ROTS, and heading.</p>
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<p>Pier masking area to classify container ships from the training dataset. Red and blue polygons display CP and NCP, respectively (left figure). A sample container ship berthed at CP on 10 February 2021 (green circle), intersecting the CP polygon and container ship trajectory points (right figure). Here, CP = container pier, and NCP = non-container pier.</p>
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<p>Analysis of container ship trajectory features from the training dataset. (<b>a</b>) Container ship density map in log scale and main navigating direction (black arrows). (<b>b</b>) Comparative analysis between container ships and other ship types using the three RGB inputs, composed of ship trajectories (b-1,b-2), SOG (b-3,b-4), and COG (b-5,b-6), respectively.</p>
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<p>Comparison of ground truth and model classification results for fishing boat (blue circle), passenger ship (green circle), and container ship (red circle).</p>
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<p>Confusion matrices of HMM, DNN, and CNN models applied to the test dataset. (<b>a</b>) Fishing boats and other ships. (<b>b</b>) Passenger ships and other ships. (<b>c</b>) Container ships and other ships.</p>
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15 pages, 6687 KiB  
Article
A Case Study of an Antibiotic Discovery Laboratory Autonomous Learning Assignment—An Evaluation of Undergraduate Students’ Disciplinary Bias
by Nadav Badrian, Lilach Iasur-Kruh, Yael Ungar and Iris Sonia Weitz
Educ. Sci. 2024, 14(11), 1176; https://doi.org/10.3390/educsci14111176 - 28 Oct 2024
Viewed by 655
Abstract
Current higher education trends are moving towards interdisciplinary curricula to provide new tools for solving complex issues. However, course design and learning tracks still create divisions between scientific disciplines. This study aimed to evaluate the disciplinary bias of second-year undergraduate students of biotechnology [...] Read more.
Current higher education trends are moving towards interdisciplinary curricula to provide new tools for solving complex issues. However, course design and learning tracks still create divisions between scientific disciplines. This study aimed to evaluate the disciplinary bias of second-year undergraduate students of biotechnology engineering in the organic chemistry laboratory class through a laboratory setting involving blended disciplines. An experiment on antibiotic discovery that integrates parallel and combinatorial organic chemistry syntheses with microbiology techniques was chosen. As a part of an activity, students had free choice in designing the arrangement of the organic compounds and the two bacterial species by setting up the layout for a 96-well plate. The study visually analyzed students’ plate layouts (n = 74) according to discipline classification and the spatial arrangements of organic compounds (e.g., products and libraries). The results identified four themes that are suggested to reflect students’ vertical, lateral, and interdisciplinary thinking, as most were found to be in the procedural knowledge range and between Bloom’s application and analysis dimensions. Using this study’s thematic analysis methodology in chemistry and related educational fields can provide a pedagogical reflective tool and advance personalized teaching and interdisciplinarity. Full article
(This article belongs to the Special Issue Challenges and Trends for Modern Higher Education)
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<p>Thematic analysis of students’ 96 well-plate designs.</p>
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<p>Schematic illustrations of plate layouts. (<b>a</b>,<b>b</b>) The material-oriented arrangement shows a chemistry viewpoint focused on separating the materials between libraries and products. (<b>c</b>,<b>d</b>) The bacteria-oriented arrangement shows microbial considerations separating the plate between bacteria. The black and red font colors represent bacterial species <span class="html-italic">E. coli</span> and <span class="html-italic">B. cereus,</span> respectively. Note: the visual arrangement of products and libraries (<b>a</b>,<b>c</b>) in squares and columns and (<b>b</b>,<b>d</b>) in rows.</p>
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<p>Classifications of plate layouts (<span class="html-italic">n</span> = 74). (<b>a</b>) Number of students categorized following theme analysis. (<b>b</b>) Percentage of students taking disciplines and visual considerations.</p>
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<p>Schematic illustrations of distinctive plate layouts showing the correlation between the products and libraries. (<b>a</b>) A matrix composed of libraries and products, all correlating. (<b>b</b>) Libraries L1, L2, and L3 correlate with the products. The black and red font colors represent bacterial species: <span class="html-italic">E. coli</span> and <span class="html-italic">B. cereus</span>, respectively.</p>
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<p>Bottom view of the 96-well plate screening results after 24 h of incubation (the dashed lines represent the series of products and libraries; each color represents a different bacterial species). The 96-well plate designs correspond with (<b>a</b>) Theme 1—materials in squares and columns, (<b>b</b>) Theme 3—bacteria in squares and columns, which relate to (<b>c</b>) the product (P1–P9) square of 3 by 3 Eppendorf tubes that represents the 3 by 3 matrix preparation, (<b>d</b>) Theme 2—materials in rows, (<b>f</b>) Theme 4—bacteria in rows which relate to (<b>e</b>) the in-line ordinal hydrazone (P and L) preparation. Note: photograph (<b>e</b>) presents the color of the hydrazone products (P1–P9) and libraries (L1–L6). Each Eppendorf tube P1–P9 contains one product, and each Eppendorf L1–L6 contains one library of three products.</p>
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<p>Condensation reaction between aldehydes (A1, A2 and A3) and hydrazines (H1, H2 and H3) to yield hydrazone derivatives products (P1–P9). The chemical structures are shown in <a href="#app2-education-14-01176" class="html-app">Appendix A</a>.</p>
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22 pages, 2862 KiB  
Article
AI-Powered Eye Tracking for Bias Detection in Online Course Reviews: A Udemy Case Study
by Hedda Martina Šola, Fayyaz Hussain Qureshi and Sarwar Khawaja
Big Data Cogn. Comput. 2024, 8(11), 144; https://doi.org/10.3390/bdcc8110144 - 25 Oct 2024
Viewed by 1256
Abstract
The rapid growth of e-learning increased the use of digital reviews to influence consumer purchases. In a pioneering approach, we employed AI-powered eye tracking to evaluate the accuracy of predictions in forecasting purchasing patterns. This study examined customer perceptions of negative, positive, and [...] Read more.
The rapid growth of e-learning increased the use of digital reviews to influence consumer purchases. In a pioneering approach, we employed AI-powered eye tracking to evaluate the accuracy of predictions in forecasting purchasing patterns. This study examined customer perceptions of negative, positive, and neutral reviews by analysing emotional valence, review content, and perceived credibility. We measured ‘Attention’, ‘Engagement’, ‘Clarity’, ‘Cognitive Demand’, ‘Time Spent’, ‘Percentage Seen’, and ‘Focus’, focusing on differences across review categories to understand their effects on customers and the correlation between these metrics and navigation to other screen areas, indicating purchasing intent. Our goal was to assess the predictive power of online reviews on future buying behaviour. We selected Udemy courses, a platform with over 70 million learners. Predict (version 1.0.), developed by Stanford University, was used with the algorithm on the consumer neuroscience database (n = 180,000) from Tobii eye tracking (Tobii X2-30, Tobii Pro AB, Danderyd, Sweden). We utilised R programming, ANOVA, and t-tests for analysis. The study concludes that AI neuromarketing techniques in digital feedback analysis offer valuable insights for educators to tailor strategies based on review susceptibility, thereby sparking interest in the innovative possibilities of using AI technology in neuromarketing. Full article
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<p>(<b>a</b>): Research flow chart. (<b>b</b>): A detailed research roadmap is derived from project development, setup, and execution.</p>
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<p>(<b>a</b>): Research flow chart. (<b>b</b>): A detailed research roadmap is derived from project development, setup, and execution.</p>
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<p>(<b>a</b>–<b>c</b>): Focus score differences between negative and positive reviews based on the video data analysis with heatmaps selected on reviews. The heat map illustrates the areas that garnered the most significant attention, while the attention itself was evaluated on a frame-by-frame basis throughout the entire video. (<b>d</b>–<b>f</b>): Cognitive Demand score differences between negative and positive reviews are based on the video data analysis with fog map selected on reviews. The fog map unambiguously reveals the areas not discernible to the human eye when recording the cognitive demand frame by frame. Consequently, the figure appears illegible.</p>
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<p>(<b>a</b>–<b>c</b>): Focus score differences between negative and positive reviews based on the video data analysis with heatmaps selected on reviews. The heat map illustrates the areas that garnered the most significant attention, while the attention itself was evaluated on a frame-by-frame basis throughout the entire video. (<b>d</b>–<b>f</b>): Cognitive Demand score differences between negative and positive reviews are based on the video data analysis with fog map selected on reviews. The fog map unambiguously reveals the areas not discernible to the human eye when recording the cognitive demand frame by frame. Consequently, the figure appears illegible.</p>
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<p>(<b>a</b>–<b>c</b>): Focus score differences between negative and positive reviews based on the video data analysis with heatmaps selected on reviews. The heat map illustrates the areas that garnered the most significant attention, while the attention itself was evaluated on a frame-by-frame basis throughout the entire video. (<b>d</b>–<b>f</b>): Cognitive Demand score differences between negative and positive reviews are based on the video data analysis with fog map selected on reviews. The fog map unambiguously reveals the areas not discernible to the human eye when recording the cognitive demand frame by frame. Consequently, the figure appears illegible.</p>
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<p>(<b>a</b>): Total Attention-derived focus heat map of the negative (2-star) review category based on the image data analysis. (<b>b</b>): Total Attention-derived heat map of the positive (5-star) review category based on the image data analysis. The ‘both’ figure represents the AOI’s selected per each review which was needed to the obtain more insightful findings.</p>
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<p>(<b>a</b>): Total Attention-derived focus heat map of the negative (2-star) review category based on the image data analysis. (<b>b</b>): Total Attention-derived heat map of the positive (5-star) review category based on the image data analysis. The ‘both’ figure represents the AOI’s selected per each review which was needed to the obtain more insightful findings.</p>
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<p>Correlation matrix for the review view of the negative (2-star) review category from the image data analysis.</p>
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24 pages, 5194 KiB  
Article
Decentralized Multi-Agent Search for Moving Targets Using Road Network Gaussian Process Regressions
by Brady Moon, Christine Akagi and Cameron K. Peterson
Drones 2024, 8(11), 606; https://doi.org/10.3390/drones8110606 - 23 Oct 2024
Viewed by 2675
Abstract
Unmanned aerial vehicles (UAVs) can collaborate as teams to accomplish diverse mission objectives, such as target search and tracking. This paper introduces a method that leverages accumulated target-density information over the course of a UAV mission to adapt path-planning rewards, guiding UAVs toward [...] Read more.
Unmanned aerial vehicles (UAVs) can collaborate as teams to accomplish diverse mission objectives, such as target search and tracking. This paper introduces a method that leverages accumulated target-density information over the course of a UAV mission to adapt path-planning rewards, guiding UAVs toward areas with a higher likelihood of target presence. The target density is modeled using a Gaussian process, which is iteratively updated as the UAVs search the environment. Unlike conventional search algorithms that prioritize unexplored regions, this approach incentivizes revisiting target-rich areas. The target-density information is shared across UAVs using decentralized consensus filters, enabling cooperative path selection that balances the exploration of uncertain regions with the exploitation of known high-density areas. The framework presented in this paper provides an adaptive cooperative search method that can quickly develop an understanding of the region’s target-dense areas, helping UAVs refine their search. Through Monte Carlo simulations, we demonstrate this method in both a 2D grid region and road networks, showing up to a 26% improvement in target density estimates. Full article
(This article belongs to the Topic Civil and Public Domain Applications of Unmanned Aviation)
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<p>This figure depicts the research objective to efficiently search for targets using UAVs by predicting the target densities along the road networks based on their history of observations.</p>
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<p>This figure provides the functional diagram of our cooperative search algorithm. This shows that once vehicles sense information from their environment and share their local common operating picture then this information is incorporated in the road segment rewards.</p>
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<p>This figure illustrates the Rollout policy for path planning, which initially employs an exhaustive search before transitioning to a greedy heuristic policy once sufficient spatial diversity is achieved among the potential paths. The darker gray circles represent the states selected for expansion by the greedy heuristic policy.</p>
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<p>An illustration of how the length scale (<math display="inline"><semantics> <mi>λ</mi> </semantics></math>) and signal variance (<math display="inline"><semantics> <msubsup> <mi>σ</mi> <mi>f</mi> <mn>2</mn> </msubsup> </semantics></math>) influence the GP regression fit using test data generated from a noisy sine wave. (<b>a</b>) Under-fit GP regression; (<b>b</b>) Good-fit GP regression; (<b>c</b>) Over-fit GP Regression.</p>
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<p>This figure shows the Chatsworth simulation environment and true heat map at time <math display="inline"><semantics> <mrow> <mi>t</mi> <mo>=</mo> <mn>600</mn> </mrow> </semantics></math> s. (<b>a</b>) Target paths and road network; (<b>b</b>) True heat map.</p>
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<p>These figures display the heat maps and UAV paths for a single simulation run across all three search methods at time <math display="inline"><semantics> <mrow> <mi>t</mi> <mo>=</mo> <mn>600</mn> </mrow> </semantics></math> s, with two UAVs conducting searches in the Chatsworth region. (<b>a</b>) UMSR Method; (<b>b</b>) HMMSR Method; (<b>c</b>) GP Regression Method; (<b>d</b>) Heat map for UMSR; (<b>e</b>) Heat map for HMMSR; (<b>f</b>) Heat map for GP regression.</p>
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<p>RMS errors for the heat map averaged over 100 MC runs for 600 s using the grid cell method in the Chatsworth region.</p>
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<p>Chatsworth as a road network.</p>
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<p>RMS errors for the heat map averaged over 100 MC runs for 600 s using the Chatsworth road network.</p>
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15 pages, 650 KiB  
Article
Marketization of Shadow Education in Switzerland: How “Edupreneurs” Promote Preparation Programmes for a Selective School Transition
by Sara Landolt and Itta Bauer
Educ. Sci. 2024, 14(11), 1143; https://doi.org/10.3390/educsci14111143 - 23 Oct 2024
Viewed by 1179
Abstract
Private supplementary tutoring (PST) is a flourishing market for edupreneurs whose services relate closely to mainstream education. While international research elaborates on geographical variations in PST and edupreneurs’ marketing strategies, the PST market in Switzerland has been largely understudied. This paper contributes to [...] Read more.
Private supplementary tutoring (PST) is a flourishing market for edupreneurs whose services relate closely to mainstream education. While international research elaborates on geographical variations in PST and edupreneurs’ marketing strategies, the PST market in Switzerland has been largely understudied. This paper contributes to fill this research gap by presenting a thematic analysis of the websites of edupreneurs offering preparation programmes for the highly selective central entrance examination (CEE) to the academically focused public school track of Gymnasium in Zurich. Conceptually, we draw on “problematization” and “commodificiation” as key terms elaborated by “geographies of marketization”. With this performative conceptualisation of the education market, we examine the marketing strategies of the edupreneurs offering CEE preparation courses. We offer two contributions to the existing research. First, the analysis elaborates that private CEE preparation courses are advertised as a market solution that compensates for an omission caused by public education. Second, we show how edupreneurs use rational and emotional arguments to convince families of the need of booking CEE preparation courses for their children. In the discussion, we engage with the findings of our local case study and argue that selective educational transitions have much wider relevance for policy and society elsewhere. Full article
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<p>Theme mapping “CEE is advertised in relation to public education”.</p>
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<p>Theme mapping “Edupreneurs offering high-quality services”.</p>
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23 pages, 7594 KiB  
Article
Spatiotemporal Point–Trace Matching Based on Multi-Dimensional Feature Fuzzy Similarity Model
by Yi Liu, Ruijie Wu, Wei Guo, Liang Huang, Kairui Li, Man Zhu and Pieter van Gelder
J. Mar. Sci. Eng. 2024, 12(10), 1883; https://doi.org/10.3390/jmse12101883 - 20 Oct 2024
Viewed by 651
Abstract
Identifying ships is essential for maritime situational awareness. Automatic identification system (AIS) data and remote sensing (RS) images provide information on ship movement and properties from different perspectives. This study develops an efficient spatiotemporal association approach that combines AIS data and RS images [...] Read more.
Identifying ships is essential for maritime situational awareness. Automatic identification system (AIS) data and remote sensing (RS) images provide information on ship movement and properties from different perspectives. This study develops an efficient spatiotemporal association approach that combines AIS data and RS images for point–track association. Ship detection and feature extraction from the RS images are performed using deep learning. The detected image characteristics and neighboring AIS data are compared using a multi-dimensional feature similarity model that considers similarities in space, time, course, and attributes. An efficient spatial–temporal association analysis of ships in RS images and AIS data is achieved using the interval type-2 fuzzy system (IT2FS) method. Finally, optical images with different resolutions and AIS records near the waters of Yokosuka Port and Kure are collected to test the proposed model. The results show that compared with the multi-factor fuzzy comprehensive decision-making method, the proposed method can achieve the best performance (F1 scores of 0.7302 and 0.9189, respectively, on GF1 and GF2 images) while maintaining a specific efficiency. This work can realize ship positioning and monitoring based on multi-source data and enhance maritime situational awareness. Full article
(This article belongs to the Section Ocean Engineering)
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<p>Image thumbnails of the experimental area. (<b>a</b>,<b>b</b>) are Yokosuka Port, (<b>c</b>,<b>d</b>) are Kure Port. (<b>a</b>,<b>c</b>) are GF1 images, (<b>b</b>,<b>d</b>) are GF2 images.</p>
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<p>Workflow for point–track association based on multi-dimensional feature similarity.</p>
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<p>Drawbacks of directly calculating the spatial distance. The red dots indicate the target points and the green and blue polylines represent the two ship tracks. (<b>a</b>) is the initial state, (<b>b</b>,<b>c</b>) are two different ways of calculating the spatial distance.</p>
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<p>Mismatching of point–track based purely on spatial feature similarity.</p>
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<p>Direction consistency assessment based on vector cosine. (<b>a</b>) and (<b>b</b>) are two cases of the directional consistency of the sailing ship.</p>
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<p>The importance of course feature similarity.</p>
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<p>Effects of wake on bounding box in different operating states: (<b>a</b>,<b>d</b>) are low speed, (<b>b</b>,<b>e</b>) are medium speed, and (<b>c</b>,<b>f</b>) are high speed.</p>
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<p>Membership function of spatiotemporal fuzzy similarity.</p>
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<p>Membership function of course fuzzy similarity.</p>
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<p>Membership function of attribute fuzzy similarity.</p>
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<p>Membership function of feature correlation degree.</p>
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<p>Ship detection results using the oriented R-CNN algorithm. (<b>a</b>,<b>b</b>) are examples from GF1 images, and (<b>c</b>,<b>d</b>) are examples from GF2 images.</p>
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<p>Method for judging the results of ship matching.</p>
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<p>Membership functions and F1 scores computed by different FOU sizes. (<b>a</b>–<b>f</b>) are membership functions at different FOU sizes, and (<b>g</b>) is the corresponding model result.</p>
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<p>Example of matching result using the proposed model. (<b>a</b>,<b>b</b>) are matching examples in this study, each polyline in the figure is drawn according to the AIS record, and the number next to it is the corresponding MMSI number.</p>
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38 pages, 6505 KiB  
Review
A Survey of Computer Vision Detection, Visual SLAM Algorithms, and Their Applications in Energy-Efficient Autonomous Systems
by Lu Chen, Gun Li, Weisi Xie, Jie Tan, Yang Li, Junfeng Pu, Lizhu Chen, Decheng Gan and Weimin Shi
Energies 2024, 17(20), 5177; https://doi.org/10.3390/en17205177 - 17 Oct 2024
Cited by 1 | Viewed by 1057
Abstract
Within the area of environmental perception, automatic navigation, object detection, and computer vision are crucial and demanding fields with many applications in modern industries, such as multi-target long-term visual tracking in automated production, defect detection, and driverless robotic vehicles. The performance of computer [...] Read more.
Within the area of environmental perception, automatic navigation, object detection, and computer vision are crucial and demanding fields with many applications in modern industries, such as multi-target long-term visual tracking in automated production, defect detection, and driverless robotic vehicles. The performance of computer vision has greatly improved recently thanks to developments in deep learning algorithms and hardware computing capabilities, which have spawned the creation of a large number of related applications. At the same time, with the rapid increase in autonomous systems in the market, energy consumption has become an increasingly critical issue in computer vision and SLAM (Simultaneous Localization and Mapping) algorithms. This paper presents the results of a detailed review of over 100 papers published over the course of two decades (1999–2024), with a primary focus on the technical advancement in computer vision. To elucidate the foundational principles, an examination of typical visual algorithms based on traditional correlation filtering was initially conducted. Subsequently, a comprehensive overview of the state-of-the-art advancements in deep learning-based computer vision techniques was compiled. Furthermore, a comparative analysis of conventional and novel algorithms was undertaken to discuss the future trends and directions of computer vision. Lastly, the feasibility of employing visual SLAM algorithms in the context of autonomous vehicles was explored. Additionally, in the context of intelligent robots for low-carbon, unmanned factories, we discussed model optimization techniques such as pruning and quantization, highlighting their importance in enhancing energy efficiency. We conducted a comprehensive comparison of the performance and energy consumption of various computer vision algorithms, with a detailed exploration of how to balance these factors and a discussion of potential future development trends. Full article
(This article belongs to the Section K: State-of-the-Art Energy Related Technologies)
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<p>The Applying Scenarios of Computer Vision.</p>
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<p>The Increasing Number of Publications in Object Detection from 2010 to 2024. (Data from IEEE Xplore advanced search: all in title: “computer vision”).</p>
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<p>A Road Map of Computer Vision with Milestone Detectors and Trackers [<a href="#B1-energies-17-05177" class="html-bibr">1</a>,<a href="#B2-energies-17-05177" class="html-bibr">2</a>,<a href="#B3-energies-17-05177" class="html-bibr">3</a>,<a href="#B4-energies-17-05177" class="html-bibr">4</a>,<a href="#B5-energies-17-05177" class="html-bibr">5</a>,<a href="#B6-energies-17-05177" class="html-bibr">6</a>,<a href="#B7-energies-17-05177" class="html-bibr">7</a>,<a href="#B8-energies-17-05177" class="html-bibr">8</a>,<a href="#B9-energies-17-05177" class="html-bibr">9</a>,<a href="#B10-energies-17-05177" class="html-bibr">10</a>,<a href="#B11-energies-17-05177" class="html-bibr">11</a>,<a href="#B12-energies-17-05177" class="html-bibr">12</a>,<a href="#B13-energies-17-05177" class="html-bibr">13</a>,<a href="#B14-energies-17-05177" class="html-bibr">14</a>,<a href="#B15-energies-17-05177" class="html-bibr">15</a>].</p>
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<p>Cascade Classifier Structure. 1, 2, 3, and 4 represent different levels of classifiers, with T standing for true and F indicating failure.</p>
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<p>Adaptive Long-Term Tracking Framework Based on Visual Detection.</p>
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<p>Positioning Accuracy Map and Coverage of System Frame.</p>
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<p>The Datasets We Have Built Ourselves in Recent Years.</p>
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<p>Network Structure of One-Stage Detector.</p>
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<p>Network Structure of Two-Stage Detector.</p>
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<p>The Steady Improvement of Accuracy in Visual Detection Algorithms on VOC Dataset.</p>
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<p>ACDet Model System Architecture.</p>
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<p>Test Results of Acdet Model on EP Dataset.</p>
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<p>A Computer Vision Detection System Based on Deep Learning Applied in the Medical Industry.</p>
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<p>Principles of Visual Odometry.</p>
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<p>Unmanned Driving System Based on Visual SLAM and Tracking Algorithms.</p>
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<p>Visual Framework for Unmanned Factory Applications with Multi-Driverless Robotic Vehicles.</p>
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11 pages, 1016 KiB  
Article
Is the Endurance Standardized ACWRHMLD or the Underlying Acute and Chronic Components Related to Injuries?
by Robert Percy Marshall, Stephan Schulze, Jan-Niklas Droste, Helge Riepenhof, Karl-Stefan Delank, Eduard Kurz and René Schwesig
Appl. Sci. 2024, 14(20), 9427; https://doi.org/10.3390/app14209427 - 16 Oct 2024
Viewed by 654
Abstract
Acute (AW) and chronic (CW) workload imbalances, including their ratio (ACWR), are largely associated with increased injury risk. However, the inclusion of personal endurance performance (EP) in this calculation as a means of improving accuracy has been neglected in previous studies. The aim [...] Read more.
Acute (AW) and chronic (CW) workload imbalances, including their ratio (ACWR), are largely associated with increased injury risk. However, the inclusion of personal endurance performance (EP) in this calculation as a means of improving accuracy has been neglected in previous studies. The aim of this longitudinal observational study was to evaluate the relevance of the high metabolic load distance (ACWRHMLD) to EP in relation to non-contact injuries. Twenty-three German male first division soccer players (age: 24.5 ± 3.5 years; VO2max: 53.7 ± 4.9 mL/min/kg; v4: 15.2 ± 0.9 km/h) were analyzed. Eleven players with non-contact injuries were identified and matched with players without any injuries within the same time interval. Players were monitored using GPS and LPS tracking to calculate ACWRHMLD on a daily basis over the course of one competitive season. Relationships between different endurance performance parameters (v2, v4, vLT, VO2max) and the ACWRHMLD, AW, CW were established for statistical analysis. An area under the curve analysis (AUC) was performed. Based on the four weeks preceding the non-contact injuries, the CW, especially for the last two weeks before the injury, proved to be the most suitable parameter to estimate the risk of injury. The highest significant AUC value (0.81, 95% CI: 0.59–1.00) was calculated for the CW (last week before injury) in relation to the vLT (suitable cut-off: 0.04 km; sensitivity: 78%, specificity: 80%). With regard to the injury rate, the ACWRHMLD seems to be the most appropriate method of calculation, especially for CW related to EP (vLT). The sole use of ACWR, AW, and CW is not recommended. Full article
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<p>(<b>a</b>,<b>b</b>). Endurance performance characteristics of players depending on playing positions. Presented are different lactate threshold speeds (<b>a</b>) and the relative maximum oxygen uptake (<b>b</b>).</p>
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<p>(<b>a</b>–<b>d</b>). Receiver operating characteristic curve (ROC) depending on week before the injury and different EP parameters related to the chronic component of ACWR. AUC values and 95% CI are reported. (<b>a</b>) chronic related to v2. (<b>b</b>) chronic related to v4. (<b>c</b>) chronic related to VO<sub>2max.</sub> (<b>d</b>) chronic related to v<sub>LT</sub>.</p>
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20 pages, 3379 KiB  
Article
A Balanced Path-Following Approach to Course Change and Original Course Convergence for Autonomous Vessels
by Won-Jin Choi and Jeong-Seok Lee
J. Mar. Sci. Eng. 2024, 12(10), 1831; https://doi.org/10.3390/jmse12101831 - 14 Oct 2024
Viewed by 802
Abstract
This paper proposes a novel path-following method for autonomous ships that optimizes overall performance by balancing course changes and convergence to the original route. The proposed method extends the line-of-sight (LOS) guidance law by dynamically adjusting key parameters based on the ship’s cross-track [...] Read more.
This paper proposes a novel path-following method for autonomous ships that optimizes overall performance by balancing course changes and convergence to the original route. The proposed method extends the line-of-sight (LOS) guidance law by dynamically adjusting key parameters based on the ship’s cross-track error (XTE) and the distance of new course considering maneuvering characteristics. By incorporating these maneuvering characteristics, the method enables more precise adjustments during course changes, improving overall path-following performance. Simulation results showed that the proposed method outperformed three existing methods, including the traditional LOS guidance law, by minimizing overshoot and maintaining reasonable XTE during larger course changes. It achieved the lowest mean absolute cross-track error (MAE) while also significantly reducing the total time required to follow the path, highlighting its superior accuracy and efficiency in path following. These outcomes highlight the method’s potential to enhance significantly the path-following capabilities of autonomous vessels, contributing to greater efficiency and accuracy in pre-determined route navigation. Full article
(This article belongs to the Special Issue Maritime Artificial Intelligence Convergence Research)
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<p>Coordinate systems.</p>
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<p>Traditional LOS guidance law.</p>
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<p>Time-varying lookahead distance guidance law.</p>
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<p>ATMM for calculating distance of new course.</p>
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<p>Block diagram of heading controller.</p>
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<p>PD controller parameter tuning: (<b>a</b>) ultimate oscillation; (<b>b</b>) step response.</p>
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<p>Turning test for KVLCC2 (<math display="inline"><semantics> <mrow> <mo>±</mo> <mn>35</mn> <mo>°</mo> </mrow> </semantics></math>).</p>
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<p>ZigZag test for KVLCC2 (<math display="inline"><semantics> <mrow> <mo>±</mo> <mn>35</mn> <mo>°</mo> </mrow> </semantics></math>).</p>
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<p>Path following simulation test results: (<b>a</b>) ship’s trajectories following the desired path; (<b>b</b>) yaw angles; (<b>c</b>) cross-track errors; (<b>d</b>) rudder angles; (<b>e</b>) speeds.</p>
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19 pages, 7151 KiB  
Article
PET Imaging with [18F]ROStrace Detects Oxidative Stress and Predicts Parkinson’s Disease Progression in Mice
by Yi Zhu, Neha Kohli, Anthony Young, Malkah Sheldon, Jani Coni, Meera Rajasekaran, Lozen Robinson, Rea Chroneos, Shaipreeah Riley, Joseph W. Guarnieri, Joshua Jose, Nisha Patel, Douglas C. Wallace, Shihong Li, Hsiaoju Lee, Robert H. Mach and Meagan J. McManus
Antioxidants 2024, 13(10), 1226; https://doi.org/10.3390/antiox13101226 - 12 Oct 2024
Viewed by 1397
Abstract
Although the precise molecular mechanisms responsible for neuronal death and motor dysfunction in late-onset Parkinson’s disease (PD) are unknown, evidence suggests that mitochondrial dysfunction and neuroinflammation occur early, leading to a collective increase in reactive oxygen species (ROS) production and oxidative stress. However, [...] Read more.
Although the precise molecular mechanisms responsible for neuronal death and motor dysfunction in late-onset Parkinson’s disease (PD) are unknown, evidence suggests that mitochondrial dysfunction and neuroinflammation occur early, leading to a collective increase in reactive oxygen species (ROS) production and oxidative stress. However, the lack of methods for tracking oxidative stress in the living brain has precluded its use as a potential biomarker. The goal of the current study is to address this need through the evaluation of the first superoxide (O2•−)-sensitive radioactive tracer, [18F]ROStrace, in a model of late-onset PD. To achieve this goal, MitoPark mice with a dopaminergic (DA) neuron-specific deletion of transcription factor A mitochondrial (Tfam) were imaged with [18F]ROStrace from the prodromal phase to the end-stage of PD-like disease. Our data demonstrate [18F]ROStrace was sensitive to increased oxidative stress during the early stages of PD-like pathology in MitoPark mice, which persisted throughout the disease course. Similarly to PD patients, MitoPark males had the most severe parkinsonian symptoms and metabolic impairment. [18F]ROStrace retention was also highest in MitoPark males, suggesting oxidative stress as a potential mechanism underlying the male sex bias of PD. Furthermore, [18F]ROStrace may provide a method to identify patients at risk of Parkinson’s before irreparable neurodegeneration occurs and enhance clinical trial design by identifying patients most likely to benefit from antioxidant therapies. Full article
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<p>Increased [<sup>18</sup>F]ROStrace retention in MitoPark mice occurred early and persisted to end-stage of disease. (<b>A</b>, left panel) Brain subregions derived from Mirrione mouse brain atlas highlighted as follows: olfactory bulb (OB; white), cortex (Ctx; light blue), basal forebrain septum (BFS; orange), hippocampus (Hip; dark green), thalamus (Th; light green), hypothalamus (HyTh; teal), superior colliculi (SC; magenta), midbrain (Mb: pink), cerebellum (Cb, yellow), and brain stem (BS; brown). (<b>A</b>, middle panel) Sagittal view of [<sup>18</sup>F]ROStrace PET images showing increased [<sup>18</sup>F]ROStrace retention in the brain of MitoPark mice at 3 and 6 months of age compared to WT mice. (<b>A</b>, right panel) Ex vivo ARG validation of [<sup>18</sup>F]ROStrace PET in 6-month-old MitoPark mice. (<b>B</b>) Quantification of [<sup>18</sup>F]ROStrace standardized uptake value (SUV) in brain subregions highlighted in (<b>A</b>). Values represent mean ± SEM, n = 6–15 per group. <span class="html-italic">p</span> values of MitoPark vs. WT determined by unpaired <span class="html-italic">t</span>-test (* <span class="html-italic">p</span> &lt; 0.05; ** <span class="html-italic">p</span> &lt; 0.01; *** <span class="html-italic">p</span> &lt; 0.001). (<b>C</b>,<b>D</b>) Linear regression analysis of WT (black dots, n = 37) and MitoPark (red triangles, n = 39) age vs. Striatum (<b>C</b>) and Midbrain (<b>D</b>) [<sup>18</sup>F]ROStrace SUV.</p>
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<p>Progressive decline of motor function in MitoPark mice. Representative images (<b>A</b>) showing the progressive reduction in distance traveled by MitoPark (right panel) compared to WT (left panel) within 15 min at 2–6 months of age. Quantitative analysis revealed a progressive decrease in motor function determined by reduced velocity (<b>B</b>), total distance traveled (<b>C</b>), number of rears (<b>D</b>), and increased time immobile (<b>E</b>) during the 15 min test. Values represent mean ± SEM, n = 15–47 per group. <span class="html-italic">p</span> values of MitoPark vs. WT determined by two-way ANOVA with mixed effects model followed by Tukey’s post-test (* <span class="html-italic">p</span> &lt; 0.05; *** <span class="html-italic">p</span> &lt; 0.001; **** <span class="html-italic">p</span> &lt; 0.0001). (<b>F</b>,<b>G</b>) Linear regression analysis of WT (black dots, n = 13) and MitoPark (red triangles, n = 23) of cumulative time in activity vs. Striatum (<b>F</b>) and Midbrain (<b>G</b>) [<sup>18</sup>F]ROStrace SUV.</p>
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<p>Gradual deterioration of metabolic function in MitoPark mice. Progressive decrease in MitoPark activity calculated as mean number of infrared (IR) beam breaks per time point within the CLAMS home cage during the dark cycle (<b>A</b>, shaded regions). Quantification analysis revealed decreased average activity in the dark cycle (<b>B</b>), respiration (delta CO<sub>2</sub>; <b>C</b>), respiratory exchange ratio (RER, VCO<sub>2</sub>/VO<sub>2</sub>; <b>D</b>) and energy expenditure (Kcal/h; <b>E</b>) in MitoPark mice over time. Bar graphs represent the mean ± SEM, n = 7–26 per group, <span class="html-italic">p</span> values of MitoPark vs. WT determined by two-way ANOVA with mixed effects model followed by Tukey’s post-test (** <span class="html-italic">p</span> &lt; 0.01; **** <span class="html-italic">p</span> &lt; 0.0001).</p>
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<p>[<sup>18</sup>F]ROStrace detects male sex bias in MitoPark mice. (<b>A</b>) Voxel-wise analyses by statistical parametric mapping (SPM; <span class="html-italic">p</span> value and threshold) showing the greatest differences in [<sup>18</sup>F]ROStrace retention in male WT vs. MitoPark brains (<b>A</b>, middle panel), with MitoPark females more closely resembling WT (<b>A</b>, lower panel). (<b>B</b>) Progressive impairment of gut motility, measured by latency to bead expulsion, in MitoPark males. (<b>C</b>) Impaired motor coordination in MitoPark males from 2–4 months of age, measured by foot slips on a tapered beam. (<b>D</b>) Decreased O<sub>2</sub> consumption by male MitoPark mice vs. WT males and MitoPark females in the home cage environment. Values represent mean ± SEM, n = 5~14 per group, <span class="html-italic">p</span> values of MitoPark vs. WT determined by two-way ANOVA followed by Tukey’s post-test. * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001, **** <span class="html-italic">p</span> &lt; 0.0001.</p>
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<p>Increased DHE signal in the substantia nigra (SN) accompanied by progressive loss of DA neurons in MitoPark mice. (<b>A</b>(<b>i</b>–<b>vi</b>)) Representative coronal images of the SN from WT (<b>A</b>(<b>i</b>–<b>iii</b>)) and MitoPark (<b>A</b>(<b>iv</b>–<b>vi</b>)) mice at 2–6 months of age showing increased DHE signal and decreased tyrosine hydroxylase (TH)-positive neurons in the MitoPark SN. Scale bar = 100 µm. (<b>B</b>) Quantification of TH-positive neurons in the SN of age-matched WT and MitoPark mouse brain tissues. Values represent mean ± SEM, n = 5–11 per group, <span class="html-italic">p</span> values of MitoPark vs. WT determined by two-way ANOVA followed by Tukey’s post-test. ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001. Western blots confirmed decreased TH in the SN (<b>C</b>,<b>D</b>) of MitoPark mice at 4–6 months of age, n = 6–11 per group, values represent mean ± SEM. <span class="html-italic">p</span> values determined by two-way ANOVA with mixed effects model followed by Tukey’s post-test. * <span class="html-italic">p</span> &lt; 0.05; ** <span class="html-italic">p</span> &lt; 0.01.</p>
Full article ">Figure 6
<p>Neuroinflammation preceded loss of DA neurons in the SN region of MitoPark mice. (<b>A</b>(<b>i</b>–<b>vi</b>)) Representative images from the WT and MitoPark SN showing infiltration of astrocytes (green) and microglia (red) surrounding DA neurons (yellow) in the SN region of MitoPark (white arrow). (<b>A</b>(<b>vii</b>–<b>xii</b>)) Representative images showing microglia (white) co-stained with CD68 (green) are increased in the SN of MitoPark at 2–4 months of age. Scale bar = 25 µm. (<b>B</b>,<b>C</b>) Quantification of astrocytes and microglia in the SN region showing an increased trend in MitoPark mice from 2–6 months of age compared to age-matched control (n = 6–11 per group, values represent mean ± SEM, <span class="html-italic">p</span> values determined by two-way ANOVA with mixed effects model followed by Tukey’s post-test). (<b>D</b>) Quantification of the % of CD68<sup>+</sup> microglial soma demonstrating enlarged lysosomal vesicles in MitoPark mice from 2–4 months of age compared to age-matched control (n = 6–11 per group, values represent mean ± SEM, <span class="html-italic">p</span> values of MitoPark vs. WT determined by two-way ANOVA with mixed effects model followed by Tukey’s post-test, * <span class="html-italic">p</span> &lt; 0.05; **** <span class="html-italic">p</span> &lt; 0.0001). (<b>E</b>) Increased circulating cell-free mitochondrial DNA (ccf-mtDNA) expression level detected by real-time PCR from plasma of WT and MitoPark mice at 2–4 months of age. n = 14–24 per group, values represent mean ± SEM, <span class="html-italic">p</span> values of MitoPark vs. WT determined by one-way ANOVA followed by Tukey’s post-test. (*** <span class="html-italic">p</span> &lt; 0.001).</p>
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