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34 pages, 90974 KiB  
Article
Multi-Decadal Land Subsidence Risk Assessment at Major Italian Cities by Integrating PSInSAR with Urban Vulnerability
by Michelle Lenardón Sánchez, Celina Anael Farías and Francesca Cigna
Land 2024, 13(12), 2103; https://doi.org/10.3390/land13122103 - 5 Dec 2024
Viewed by 439
Abstract
This study assesses subsidence-induced risk to urban infrastructure in three major Italian cities—Rome, Bologna, and Florence—by integrating satellite-based persistent scatterer interferometric synthetic aperture radar (PSInSAR) ground displacement data with urban vulnerability metrics into a novel risk assessment workflow, incorporating land use and population [...] Read more.
This study assesses subsidence-induced risk to urban infrastructure in three major Italian cities—Rome, Bologna, and Florence—by integrating satellite-based persistent scatterer interferometric synthetic aperture radar (PSInSAR) ground displacement data with urban vulnerability metrics into a novel risk assessment workflow, incorporating land use and population data from the Copernicus Land Monitoring Service (CLMS)—Urban Atlas. This analysis exploits ERS-1/2, ENVISAT, and COSMO-SkyMed PSInSAR datasets from the Italian Extraordinary Plan of Environmental Remote Sensing, plus Sentinel-1 datasets from CLMS—European Ground Motion Service (EGMS), and spans a 30-year period, thus capturing both historical and recent subsidence trends. Angular distortion is introduced as a critical parameter for assessing potential structural damage due to differential settlement, which helps to quantify subsidence-induced hazards more precisely. The results reveal variable subsidence hazard patterns across the three cities, with specific areas exhibiting significant differential ground deformation that poses risks to key infrastructure. A total of 36.15, 11.44, and 0.43 km2 of land at high to very high risk are identified in Rome, Bologna, and Florence, respectively. By integrating geospatial and vulnerability data at the building-block level, this study offers a more comprehensive understanding of subsidence-induced risk, potentially contributing to improved management and mitigation strategies in urban areas. This study contributes to the limited literature on embedding PSInSAR data into urban risk assessment workflows and provides a replicable framework for future applications in other urban areas. Full article
(This article belongs to the Special Issue Assessing Land Subsidence Using Remote Sensing Data)
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Figure 1

Figure 1
<p>Location and land use/cover of the cities of (<b>a</b>) Rome; (<b>b</b>) Bologna, and (<b>c</b>) Florence in Italy, according to the Copernicus Urban Atlas (UA) 2018 dataset [<a href="#B24-land-13-02103" class="html-bibr">24</a>]. Land use/cover types associated with UA codes are provided in <a href="#land-13-02103-t001" class="html-table">Table 1</a>.</p>
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<p>Comparison of the vertical displacement velocity derived using the Sentinel-1 datasets, for (<b>a</b>) Rome, (<b>b</b>) Bologna, and (<b>c</b>) Florence. The linear regression between ascending and descending mode geometries is represented with a dashed yellow line. Notation: ‘Asc.’, ascending; ‘Desc.’, descending; ‘Sum’, combined ascending and descending.</p>
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<p>Vertical displacement velocity (<span class="html-italic">V<sub>U</sub></span>) maps of the 2018–2022 period for the city of Rome (<b>a</b>), Bologna (<b>b</b>), and Florence (<b>c</b>). PSInSAR data are overlapped onto Google Earth imagery.</p>
Full article ">Figure 3 Cont.
<p>Vertical displacement velocity (<span class="html-italic">V<sub>U</sub></span>) maps of the 2018–2022 period for the city of Rome (<b>a</b>), Bologna (<b>b</b>), and Florence (<b>c</b>). PSInSAR data are overlapped onto Google Earth imagery.</p>
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<p>Vertical displacement (<span class="html-italic">d<sub>U</sub></span>) and yearly vertical displacement velocities (<span class="html-italic">V<sub>U</sub></span>) time series corresponding to the sample locations selected for (<b>a</b>) Rome, (<b>b</b>) Bologna, and (<b>c</b>) Florence.</p>
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<p>Rome hazard maps, representing (<b>a</b>) the 30-year period angular distortion, <span class="html-italic">β</span><sub><span class="html-italic">T</span></sub>, and (<b>b</b>) the 2018–2022 period angular distortion, <span class="html-italic">β</span><sub>2018–2022</sub>. The 2018 Urban Atlas layer is superimposed and zooms (I) and (II) are overlapped onto the OpenStreetMap on grey scale.</p>
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<p>Bologna hazard maps, representing (<b>a</b>) the 30-year period angular distortion, <span class="html-italic">β</span><sub><span class="html-italic">T</span></sub>, and (<b>b</b>) the 2018–2022 period angular distortion, <span class="html-italic">β</span><sub>2018–2022</sub>. The 2018 Urban Atlas layer is superimposed and zooms (I) and (II) are overlapped onto the OpenStreetMap on grey scale.</p>
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<p>Florence hazard maps, representing (<b>a</b>) the 30-year period angular distortion, <span class="html-italic">β</span><sub><span class="html-italic">T</span></sub>, and (<b>b</b>) the 2018–2022 period angular distortion, <span class="html-italic">β</span><sub>2018–2022</sub>. The 2018 Urban Atlas layer is superimposed and zooms (I) and (II) are overlapped onto the OpenStreetMap on grey scale.</p>
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<p>Exposure–vulnerability of urban infrastructure in (<b>a</b>) Rome, (<b>b</b>) Bologna, and (<b>c</b>) Florence, based on scoring of UA 2018 classes.</p>
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<p>Subsidence-induced risk in Rome, assessed by exploiting the risk matrix combining angular distortion and exposure–vulnerability scores: risk mapping referred to (<b>a</b>) the 30-year period 1992–2022, and (<b>b</b>) the 2018–2022 period. Risk maps are overlapped onto Google Earth imagery.</p>
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<p>Subsidence-induced risk in Bologna, assessed by exploiting the risk matrix combining angular distortion and exposure–vulnerability scores: risk mapping referred to (<b>a</b>) the 30-year period 1992–2022, and (<b>b</b>) the 2018–2022 period. Risk maps are overlapped onto Google Earth imagery.</p>
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<p>Subsidence-induced risk in Florence, assessed by exploiting the risk matrix combining angular distortion and exposure–vulnerability scores: risk mapping referred to (<b>a</b>) the 30-year period 1992–2022, and (<b>b</b>) the 2018–2022 period. Risk maps are overlapped onto Google Earth imagery.</p>
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<p>Overview of the subsidence-induced risk mapping statistics for Rome, Bologna, and Florence in 1992–2022, highlighting the amount of square kilometers of land and population involved in each risk category. Note that each bar represents 100% of the area of each city.</p>
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<p>Example of correlation between lithology, subsidence, and resulting risk in Rome. (<b>a</b>) Lithology map representing (1) alluvial fan deposits, (2) pozzolana, (3) marginal sandy facies, (4) anthropogenic deposits, and (5) river, obtained from Lazio Region open data catalog; (<b>b</b>) total angular distortion values with Urban Atlas polygons superimposed; and (<b>c</b>) risk map for the 1992–2022 period. Notation: VH, very high; H, high; M, medium; L, low; ND, no data; NA, not applicable.</p>
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<p>Example of correlation between groundwater extraction, subsidence, and derived risk in Bologna: (<b>a</b>) 2018–2022 angular distortion values with Urban Atlas polygons superimposed. The orange dot represents the position of ARPAE’S groundwater monitoring well (modified from [<a href="#B38-land-13-02103" class="html-bibr">38</a>]). The recorded change in its piezometric level is −11.45 m. (<b>b</b>) Exposure–vulnerability of urban infrastructure. (<b>c</b>) Risk map for the 2018–2022 period.</p>
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<p>Example of correlation between landslide processes and ground displacement measured in Florence. (<b>a</b>) Shows the landslides in the study area, obtained from the Italian Landslide Inventory (IFFI project), (<b>b</b>) total angular distortion map, and (<b>c</b>) the risk matrix map.</p>
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<p>Vertical displacement velocities of Rome in: (<b>a</b>) 1992–2000 (ERS-1/2 datasets), (<b>b</b>) 2002–2010 (ENVISAT datasets), and (<b>c</b>) 2013–2014 (COSMO-SkyMed dataset). PSInSAR data are overlapped onto Google Earth imagery.</p>
Full article ">Figure A1 Cont.
<p>Vertical displacement velocities of Rome in: (<b>a</b>) 1992–2000 (ERS-1/2 datasets), (<b>b</b>) 2002–2010 (ENVISAT datasets), and (<b>c</b>) 2013–2014 (COSMO-SkyMed dataset). PSInSAR data are overlapped onto Google Earth imagery.</p>
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<p>Vertical displacement velocities of Bologna in: (<b>a</b>) 1992–2000 (ERS-1/2 datasets), and (<b>b</b>) 2002–2010 (ENVISAT datasets). PSInSAR data are overlapped onto Google Earth imagery.</p>
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<p>Vertical displacement velocities of Bologna in: (<b>a</b>) 1992–2000 (ERS-1/2 datasets), and (<b>b</b>) 2002–2010 (ENVISAT datasets). PSInSAR data are overlapped onto Google Earth imagery.</p>
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<p>Vertical displacement velocities of Florence in: (<b>a</b>) 1992–2000 (ERS-1/2 datasets), and (<b>b</b>) 2002–2010 (ENVISAT datasets). PSInSAR data are overlapped onto Google Earth imagery.</p>
Full article ">Figure A3 Cont.
<p>Vertical displacement velocities of Florence in: (<b>a</b>) 1992–2000 (ERS-1/2 datasets), and (<b>b</b>) 2002–2010 (ENVISAT datasets). PSInSAR data are overlapped onto Google Earth imagery.</p>
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<p>Rome hazard maps, representing (<b>a</b>) the 1992–2000 period angular distortion, <span class="html-italic">β</span><sub>1992–2000</sub>, (<b>b</b>) the 2003–2010 period angular distortion, <span class="html-italic">β</span><sub>2003–2010</sub>, and (<b>c</b>) the 2011–2014 period angular distortion, <span class="html-italic">β</span><sub>2011–2014</sub>. The 2018 Urban Atlas layer is superimposed and zooms (I) and (II) are overlapped onto the OpenStreetMap on grey scale.</p>
Full article ">Figure A4 Cont.
<p>Rome hazard maps, representing (<b>a</b>) the 1992–2000 period angular distortion, <span class="html-italic">β</span><sub>1992–2000</sub>, (<b>b</b>) the 2003–2010 period angular distortion, <span class="html-italic">β</span><sub>2003–2010</sub>, and (<b>c</b>) the 2011–2014 period angular distortion, <span class="html-italic">β</span><sub>2011–2014</sub>. The 2018 Urban Atlas layer is superimposed and zooms (I) and (II) are overlapped onto the OpenStreetMap on grey scale.</p>
Full article ">Figure A5
<p>Bologna hazard maps, representing (<b>a</b>) the 1992–2000 period angular distortion, <span class="html-italic">β</span><sub>1992–2000</sub> and (<b>b</b>) the 2003–2010 period angular distortion, <span class="html-italic">β</span><sub>2003–2010</sub>. The 2018 Urban Atlas layer is superimposed and zooms (I) and (II) are overlapped onto the OpenStreetMap on grey scale.</p>
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<p>Florence hazard maps, representing (<b>a</b>) the 1992–2000 period angular distortion, <span class="html-italic">β</span><sub>1992–2000</sub> and (<b>b</b>) the 2003–2010 period angular distortion, <span class="html-italic">β</span><sub>2003–2010</sub>. The 2018 Urban Atlas layer is superimposed and zooms (I) and (II) are overlapped onto the OpenStreetMap on grey scale.</p>
Full article ">Figure A6 Cont.
<p>Florence hazard maps, representing (<b>a</b>) the 1992–2000 period angular distortion, <span class="html-italic">β</span><sub>1992–2000</sub> and (<b>b</b>) the 2003–2010 period angular distortion, <span class="html-italic">β</span><sub>2003–2010</sub>. The 2018 Urban Atlas layer is superimposed and zooms (I) and (II) are overlapped onto the OpenStreetMap on grey scale.</p>
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20 pages, 10429 KiB  
Article
Dynamic Geo-Visualization of Urban Land Subsidence and Land Cover Data Using PS-InSAR and Google Earth Engine (GEE) for Spatial Planning Assessment
by Joko Widodo, Edy Trihatmoko, Muhammad Rokhis Khomarudin, Mohammad Ardha, Udhi Catur Nugroho, Nugraheni Setyaningrum, Galih Prasetya Dinanta, Rahmat Arief, Andie Setiyoko, Dandy Aditya Novresiandi, Rendi Handika, Muhammad Priyatna, Shinichi Sobue, Dwi Sarah and Wawan Hermawan
Urban Sci. 2024, 8(4), 234; https://doi.org/10.3390/urbansci8040234 - 1 Dec 2024
Viewed by 1010
Abstract
The North Java coastal area, known as the Pantura region, is experiencing significant land subsidence, with certain areas sinking up to 10 cm per year. Pekalongan is among the most affected, with subsidence rates between 10 and 19 cm annually, mainly due to [...] Read more.
The North Java coastal area, known as the Pantura region, is experiencing significant land subsidence, with certain areas sinking up to 10 cm per year. Pekalongan is among the most affected, with subsidence rates between 10 and 19 cm annually, mainly due to groundwater extraction, sediment compaction, and coastal erosion. Other coastal cities, like Semarang and Demak, show rates averaging 4 to 10 cm per year. This rapid subsidence is due to favorable geological conditions and ongoing urban development. This study investigates land subsidence in Pekalongan using the PS-InSAR method and dynamic visualization of time-series land cover data. PS-InSAR was applied to 45 scenes from ALOS-2 PALSAR-2 to monitor subsidence from 2014 to 2022. The results were validated with in situ subsidence benchmarks. Urban development dynamics were analyzed through land cover and land use change (LULC) and population density over the same period, using the GLC_FCS30D dataset in the GEE to detect non-natural LULC. The PS-InSAR results indicated that over 60.9% of investigation points experienced subsidence, up to 100 cm between 2014 and 2022. Ground validation showed an 83% agreement with PS-InSAR results. A statistical analysis of LULC from 2014 to 2022 did not show significant built-up area development, but the extension of salt marshes and water bodies indicated subsidence expansion. The population density reached 6873 people per square km by 2022, causing extensive groundwater use for domestic and industrial purposes, further aggravating the subsidence. Full article
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<p>An example of urban change in Pekalongan City is derived from LANDSAT data comparing 1993 (<b>a</b>) and 2019 (<b>b</b>).</p>
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<p>Research location (land area clipped from the ALOS-2 scene utilized in the study).</p>
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<p>PS-InSAR records distribution and its velocity (in cm) during 2014–2022.</p>
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<p>The velocity of the land subsidence within the research period using 17,121 records.</p>
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<p>A graph of the accuracy test results for ALOS-2 data and field observations shows an <span class="html-italic">NSE</span> value of 0.83.</p>
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<p>LULC within the research period (2014–2022) in the research location.</p>
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<p>The LULC comparison in 2014 and 2022.</p>
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<p>Pixel number changes for LULC in 2014 and 2022.</p>
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<p>Pekalongan city’s spatial plan.</p>
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25 pages, 41258 KiB  
Article
The Deformation Monitoring Capability of Fucheng-1 Time-Series InSAR
by Zhouhang Wu, Wenjun Zhang, Jialun Cai, Hongyao Xiang, Jing Fan and Xiaomeng Wang
Sensors 2024, 24(23), 7604; https://doi.org/10.3390/s24237604 - 28 Nov 2024
Viewed by 413
Abstract
The Fucheng-1 (FC-1) satellite has successfully transitioned from its initial operational phase and is now undergoing a detailed performance assessment for time-series deformation monitoring. This study evaluates the surface deformation monitoring capabilities of the newly launched FC-1 satellite using the interferometric synthetic aperture [...] Read more.
The Fucheng-1 (FC-1) satellite has successfully transitioned from its initial operational phase and is now undergoing a detailed performance assessment for time-series deformation monitoring. This study evaluates the surface deformation monitoring capabilities of the newly launched FC-1 satellite using the interferometric synthetic aperture radar (InSAR) technique, particularly in urban applications. By analyzing the observation data from 20 FC-1 scenes and 20 Sentinel-1 scenes, deformation velocity maps of a university in Mianyang city were obtained using persistent scatterer interferometry (PSI) and distributed scatterer interferometry (DSI) techniques. The results show that thanks to the high resolution of 3 × 3 m of the FC-1 satellite, significantly more PS points and DS points were detected than those detected by Sentinel-1, by 13.4 times and 17.9 times, respectively. The distribution of the major deformation areas detected by both satellites in the velocity maps is generally consistent. FC-1 performs better than Sentinel-1 in monitoring densely structured and vegetation-covered areas. Its deformation monitoring capability at the millimeter level was further validated through comparison with leveling measurements, with average errors and root mean square errors of 1.761 mm and 2.172 mm, respectively. Its high-resolution and high-precision interferometry capabilities make it particularly promising in the commercial remote sensing market. Full article
(This article belongs to the Special Issue Recent Advances in Synthetic Aperture Radar (SAR) Remote Sensing)
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<p>(<b>a</b>) Coverage areas of Sentinel-1 (purple) and FC-1 (brown), study area location marked by a five-pointed star, and COPDEM topographic map. (<b>b</b>) Google Maps image of the study area.</p>
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<p>Flow chart of DSI and PSI.</p>
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<p>(<b>a</b>) Spatio-temporal baseline map of FC-1 single master image. (<b>b</b>) Spatio-temporal baseline map of Sentinel-1 single master image.</p>
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<p>(<b>a</b>,<b>b</b>) Vertical deformation velocity maps from FC-1 using the DSI and PSI methods. (<b>c</b>,<b>d</b>) Vertical deformation velocity maps from Sentinel-1 using the DSI and PSI methods. (<b>e</b>) Drone orthophoto of the reference point.</p>
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<p>(<b>a</b>,<b>b</b>) Histograms of deformation velocity from FC-1 using the DSI and PSI methods. (<b>c</b>,<b>d</b>) Histograms of deformation velocity from Sentinel-1 using the DSI and PSI methods.</p>
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<p>(<b>a</b>) Schematic diagram of the research area on Google Earth. (<b>b</b>,<b>c</b>) Deformation rate maps of region R1 obtained by FC-1 and Sentinel-1 using the PSI method, with a drone image as the base map. (<b>d</b>–<b>g</b>) Deformation rate maps of regions R2 and R3 obtained by FC-1 and Sentinel-1 using the PSI method, with Google Earth as the base map. (<b>h</b>–<b>k</b>) Deformation rate maps of regions R4 and R5 obtained by FC-1 and Sentinel-1 using the DSI method, with Google Earth or a drone image as the base map.</p>
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<p>(<b>a</b>) Deformation velocity points obtained by FC-1 using the PSI method overlaid onto a drone image. (<b>b</b>) Deformation velocity points obtained by Sentinel-1 using the PSI method overlaid onto a drone image.</p>
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<p>(<b>a</b>,<b>d</b>) Deformation velocity maps from FC-1 and Sentinel-1 using the PSI method, with schematic maps of ZZ1 and ZZ2 locations. (<b>b</b>,<b>c</b>) PS deformation points from FC-1 overlaid onto drone oblique images of ZZ1 and ZZ2. (<b>e</b>,<b>f</b>) PS deformation points from Sentinel-1 overlaid onto drone oblique images of ZZ1 and ZZ2.</p>
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<p>(<b>a</b>,<b>b</b>) Deformation velocity points obtained by FC-1 using DSI and PSI methods overlaid onto Google imagery. (<b>c</b>,<b>d</b>) Deformation velocity points obtained by Sentinel-1 using DSI and PSI methods overlaid onto Google imagery.</p>
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<p>Diagram of road profile location.</p>
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<p>(<b>a</b>,<b>b</b>) Deformation velocity profile of FC-1 under the DSI and PSI methods. (<b>c</b>,<b>d</b>) Deformation velocity profile of Sentinel-1 under the DSI and PSI methods.</p>
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<p>(<b>a</b>,<b>b</b>) Deformation rate profiles of Sentinel-1 and FC-1 under the DSI method. (<b>c</b>) Diagram of position of vegetation section line. (<b>d</b>) UAV 3D model of vegetation area.</p>
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<p>(<b>a</b>,<b>b</b>) Coherence histograms and average coherence values for the PSI method with FC-1 and Sentinel-1. (<b>c</b>,<b>d</b>) Coherence histograms and average coherence values for the DSI method with FC-1 and Sentinel-1.</p>
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<p>(<b>a</b>,<b>b</b>) Standard deviation maps of deformation velocity for Sentinel-1 using PSI and DSI methods. (<b>c</b>,<b>d</b>) Standard deviation maps of deformation velocity for FC-1 using PSI and DSI methods.</p>
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<p>(<b>a</b>) Diagram of locations of four regions A, B, C and D. (<b>b</b>–<b>e</b>) Time-series settlement maps of FC-1 and Sentinel-1 using the DSI and PSI methods in four regions.</p>
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<p>(<b>a</b>–<b>d</b>) Spearman’s correlation matrix heatmaps of the time-series settlement amounts obtained by FC-1 and Sentinel-1 using the DSI and PSI methods in four regions.</p>
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<p>(<b>a</b>–<b>d</b>) Pearson’s correlation matrix plots of the time-series subsidence values between FC-1 and Sentinel-1 using the DSI and PSI methods in four regions.</p>
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<p>Illustrative Google Earth map showing the locations of level points.</p>
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<p>The subsidence measured by FC-1 using the DSI method compared to the subsidence measured by leveling.</p>
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<p>The subsidence measured by FC-1 and Sentinel-1 using the DSI method compared to the subsidence measured by leveling.</p>
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33 pages, 4423 KiB  
Article
Harnessing Deep Learning for Real-Time Water Quality Assessment: A Sustainable Solution
by Selma Toumi, Sabrina Lekmine, Nabil Touzout, Hamza Moussa, Noureddine Elboughdiri, Reguia Boudraa, Ouided Benslama, Mohammed Kebir, Subhan Danish, Jie Zhang, Abdeltif Amrane and Hichem Tahraoui
Water 2024, 16(23), 3380; https://doi.org/10.3390/w16233380 - 24 Nov 2024
Viewed by 753
Abstract
This study presents an innovative approach utilizing artificial intelligence (AI) for the prediction and classification of water quality parameters based on physico-chemical measurements. The primary objective was to enhance the accuracy, speed, and accessibility of water quality monitoring. Data collected from various water [...] Read more.
This study presents an innovative approach utilizing artificial intelligence (AI) for the prediction and classification of water quality parameters based on physico-chemical measurements. The primary objective was to enhance the accuracy, speed, and accessibility of water quality monitoring. Data collected from various water samples in Algeria were analyzed to determine key parameters such as conductivity, turbidity, pH, and total dissolved solids (TDS). These measurements were integrated into deep neural networks (DNNs) to predict indices such as the sodium adsorption ratio (SAR), magnesium hazard (MH), sodium percentage (SP), Kelley’s ratio (KR), potential salinity (PS), exchangeable sodium percentage (ESP), as well as Water Quality Index (WQI) and Irrigation Water Quality Index (IWQI). The DNNs model, optimized through the selection of various activation functions and hidden layers, demonstrated high precision, with a correlation coefficient (R) of 0.9994 and a low root mean square error (RMSE) of 0.0020. This AI-driven methodology significantly reduces the reliance on traditional laboratory analyses, offering real-time water quality assessments that are adaptable to local conditions and environmentally sustainable. This approach provides a practical solution for water resource managers, particularly in resource-limited regions, to efficiently monitor water quality and make informed decisions for public health and agricultural applications. Full article
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<p>Mapping of sampling points in Médéa, Algeria.</p>
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<p>Detailed diagram illustrating the development and optimization process of Deep Neural Networks (DNNs).</p>
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<p>The architecture of the optimal DNNs model.</p>
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<p>Comparison between experimental and predicted values: (<b>a</b>) Training phase, (<b>b</b>) Validation phase, and (<b>c</b>) All phases.</p>
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<p>Comparison between experimental and predicted values: (<b>a</b>) Training phase, (<b>b</b>) Validation phase, and (<b>c</b>) All phases.</p>
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<p>Comparison between experimental and predicted values for test data.</p>
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<p>Analysis of residuals using multiple techniques based on estimated values: (<b>a</b>) Comparison of experimental data with predicted values, and (<b>b</b>) Histogram of the frequency distribution of residuals.</p>
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<p>Analysis of residuals using multiple techniques based on estimated values: (<b>a</b>) Comparison of experimental data with predicted values, and (<b>b</b>) Histogram of the frequency distribution of residuals.</p>
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<p>Application for prediction and classification of water quality.</p>
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22 pages, 42906 KiB  
Article
Monitoring the Subsidence in Wan’an Town of Deyang Based on PS-InSAR Technology (Sichuan, China)
by Hongyi Guo, Antonio Miguel Martínez-Graña and José Angel González-Delgado
Sustainability 2024, 16(22), 10010; https://doi.org/10.3390/su162210010 - 16 Nov 2024
Viewed by 724
Abstract
In recent years, land subsidence has become a crucial factor affecting urban safety and sustainable development, especially in Wan’an Town. To accurately monitor and analyze the land subsidence in Wan’an Town, this study uses the PS-InSAR technique combined with an improved DEM for [...] Read more.
In recent years, land subsidence has become a crucial factor affecting urban safety and sustainable development, especially in Wan’an Town. To accurately monitor and analyze the land subsidence in Wan’an Town, this study uses the PS-InSAR technique combined with an improved DEM for detailed research on land subsidence in Wan’an Town. PS-InSAR, or Permanent Scatterer Interferometric SAR, is suitable for high-precision monitoring of surface deformation. The natural neighbor interpolation method optimizes DEM data, improving its spatial resolution and accuracy. In this study, multiple periods of SAR imagery data of Wan’an Town were collected and preprocessed through radiometric calibration, phase unwrapping, and other steps. Using the PS-InSAR technique, the phase information of permanent scatterers (PS points) on the surface was extracted to establish a deformation model and preliminarily analyze the land subsidence in Wan’an Town. Concurrently, the DEM data were optimized using the natural neighbor interpolation method to enhance its accuracy. Finally, the optimized DEM data were combined with the surface deformation information extracted through the PS-InSAR technique for a detailed analysis of the land subsidence in Wan’an Town. The research results indicate that the DEM data optimized by the natural neighbor interpolation method have higher accuracy and spatial resolution, providing a more accurate reflection of the topographical features of Wan’an Town. The research found that the optimized DEM provided a more accurate reflection of Wan’an Town’s topographical features. By combining PS-InSAR data, subsidence information from 2016 to 2024 was calculated. The study area showed varying degrees of subsidence, with rates ranging from 6 mm/year to 10 mm/year. Four characteristic deformation areas were analyzed for causes and influencing factors. The findings contribute to understanding urban land subsidence, guiding urban planning, and providing data support for geological disaster warning and prevention. Full article
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<p>Digital elevation model of the study area.</p>
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<p>Topography of the study area and radar image coverage area.</p>
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<p>Geology map of the study area.</p>
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<p>Elevation contrast chart.</p>
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<p>Workflow of PS processing.</p>
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<p>Spatial and temporal baseline distribution map.</p>
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<p>Differential interferogram.</p>
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<p>Spatial distribution of the average subsidence rate in the study area from 2016 to 2024.</p>
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<p>Settlement comparison diagram.</p>
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<p>Total subsidence in the study area from 2016 to 2024.</p>
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<p>Natural neighbor interpolation.</p>
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<p>Time-series deformation map of the study area from 2016 to 2024.</p>
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<p>GPS survey map. (<b>A</b>) Field survey map of target A, (<b>B</b>) field survey map of target B, (<b>C</b>) field survey map of target C, (<b>D</b>) field survey map of target D.</p>
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<p>(<b>A</b>) Deformation analysis of Deformation Target Area A. (<b>B</b>) Deformation analysis of Deformation Target Area B. (<b>C</b>) Deformation analysis of Deformation Target Area C. (<b>D</b>) Deformation analysis of Deformation Target Area D.</p>
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<p>(<b>A</b>) Deformation analysis of Deformation Target Area A. (<b>B</b>) Deformation analysis of Deformation Target Area B. (<b>C</b>) Deformation analysis of Deformation Target Area C. (<b>D</b>) Deformation analysis of Deformation Target Area D.</p>
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28 pages, 6037 KiB  
Article
Statistical and Independent Component Analysis of Sentinel-1 InSAR Time Series to Assess Land Subsidence Trends
by Celina Anael Farías, Michelle Lenardón Sánchez, Roberta Bonì and Francesca Cigna
Remote Sens. 2024, 16(21), 4066; https://doi.org/10.3390/rs16214066 - 31 Oct 2024
Viewed by 1055
Abstract
Advanced statistics can enable the detailed characterization of ground deformation time series, which is a fundamental step for thoroughly understanding the phenomena of land subsidence and their main drivers. This study presents a novel methodological approach based on pre-existing open-access statistical tools to [...] Read more.
Advanced statistics can enable the detailed characterization of ground deformation time series, which is a fundamental step for thoroughly understanding the phenomena of land subsidence and their main drivers. This study presents a novel methodological approach based on pre-existing open-access statistical tools to exploit satellite differential interferometric synthetic aperture radar (DInSAR) data to investigate land subsidence processes, using European Ground Motion Service (EGMS) Sentinel-1 DInSAR 2018−2022 datasets. The workflow involves the implementation of Persistent Scatterers (PS) time series classification through the PS-Time tool, deformation signal decomposition via independent component analysis (ICA), and drivers’ investigation through spatio-temporal correlation with geospatial and monitoring data. Subsidence time series at the three demonstration sites of Bologna, Ravenna and Carpi (Po Plain, Italy) were classified into linear and nonlinear (quadratic, discontinuous, uncorrelated) categories, and the mixed deformation signal of each PS was decomposed into independent components, allowing the identification of new spatial clusters with linear, accelerating/decelerating, and seasonal trends. The relationship between the different independent components and DInSAR-derived displacement velocity, acceleration, and seasonality was also analyzed via regression analysis. Correlation with geological and groundwater monitoring data supported the investigation of the relationship between the observed deformation and subsidence drivers, such as aquifer resource exploitation, local geological setting, and gas extraction/reinjection. Full article
(This article belongs to the Special Issue Monitoring Geohazard from Synthetic Aperture Radar Interferometry)
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<p>The study areas of (1) Ravenna, (2) Bologna, and (3) Carpi–Correggio–Soliera: (<b>a</b>) geographical location in Italy; (<b>b</b>) extent of the European Ground Motion Service (EGMS) Level-3 (L3) and Level-2b (L2b) dataset footprints used for the statistical analysis, overlapped onto the Copernicus Global Digital Elevation Model [<a href="#B44-remotesensing-16-04066" class="html-bibr">44</a>]; and (<b>c</b>) detail of the mean vertical deformation velocity from EGMS L3 datasets, overlapped onto a Google satellite imagery basemap.</p>
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<p>(<b>a</b>) Mean LOS deformation velocity; (<b>b</b>) acceleration; (<b>c</b>) annual seasonality amplitude; and (<b>d</b>) PS-Time classification maps for Ravenna, overlapped onto Google satellite imagery. The area selected for the following ICA analysis is highlighted on (<b>a</b>). DCV = discontinuous with constant velocity; DVV = discontinuous with variable velocity.</p>
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<p>(<b>a</b>) Mean LOS deformation velocity; (<b>b</b>) acceleration; (<b>c</b>) annual seasonality amplitude; and (<b>d</b>) PS-Time classification maps for Bologna, overlapped onto Google satellite imagery, with indication of the administrative boundary of the city of Bologna (black polygon). The rectangles (i.e., 1 in (<b>c</b>), and 2 in (<b>b</b>)) indicate the testing areas utilized in the following ICA analysis. DCV = discontinuous with constant velocity; DVV = discontinuous with variable velocity.</p>
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<p>(<b>a</b>) Mean LOS deformation velocity; (<b>b</b>) acceleration; (<b>c</b>) annual seasonality amplitude; and (<b>d</b>) PS-Time classification maps in the Carpi–Correggio–Soliera area, overlapped onto Google satellite imagery. The area selected for the following ICA analysis is highlighted on (<b>a</b>). DCV = discontinuous with constant velocity; DVV = discontinuous with variable velocity.</p>
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<p>Independent components identified in Ravenna (Ra) testing area, overlapped onto Google satellite imagery.</p>
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<p>Independent components identified in Bologna (Bo), covering (<b>a</b>) Area 1, and (<b>b</b>) Area 2, overlapped onto Google satellite imagery.</p>
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<p>Independent components identified in Soliera (So), overlapped onto Google satellite imagery.</p>
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<p>Correlation between mean deformation velocity, acceleration and amplitude of the APC, from EGMS products and PS-Time analysis, and the ICs retained for the area of Ravenna. Linear or bilinear fitting (red lines) and R<sup>2</sup> values are shown in the graphs that show the best correlation.</p>
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<p>Correlation between mean deformation velocity, acceleration, and amplitude of the APC, from EGMS products and PS-Time analysis, and the ICs retained for Area 1 in Bologna. Linear or quadratic fitting (red lines) and R2 values are shown in the graphs that show the best correlation.</p>
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<p>Correlation between mean deformation velocity, acceleration, and amplitude of the APC, from EGMS products and PS-Time analysis, and the ICs retained for Area 2 in Bologna. Linear or quadratic fitting (red lines) and R<sup>2</sup> values are shown in the graphs that show the best correlation.</p>
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<p>Correlation between mean deformation velocity, acceleration, and amplitude of the APC, from EGMS products and PS-Time analysis, and the ICs retained for the area of Soliera. Linear or quadratic fitting (red lines) and R<sup>2</sup> values are shown in the graphs that show the best correlation.</p>
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<p>(<b>a</b>) Geological map with the location of the principal gas fields operating near the coast of Ravenna and two ARPAE groundwater monitoring wells, overlapped onto Google satellite imagery; (<b>b</b>) Comparison between piezometric level variations in ARPAE’s monitoring wells RA49-00 and RA29-00 and the deformation time series of contiguous points; (<b>c</b>) Deformation velocities observed within each lithological unit, expressed in [mm/year]. Gas exploitation data in (<b>a</b>) is made available by the Italian Ministry of Environment and Energy Security [<a href="#B58-remotesensing-16-04066" class="html-bibr">58</a>], while the location of the monitoring wells and the geological layers were downloaded from the MinERva Portal, managed by the Emilia-Romagna Region service [<a href="#B46-remotesensing-16-04066" class="html-bibr">46</a>].</p>
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<p>(<b>a</b>) Position of ARPAE’S groundwater monitoring wells and the recorded change in piezometric levels (Δ<span class="html-italic">h<sub>i</sub></span>) for the area of Bologna during the studied time period (2018−2022), overlapped onto Google Satellite imagery; (<b>b</b>) Comparison between piezometric level variations in three of the wells and the deformation time series of contiguous PS–DS points; (<b>c</b>) Geological map of Bologna; (<b>d</b>) Deformation velocities observed within each lithological unit, ex-pressed in [mm/year]. Geological layers used in (<b>c</b>) were downloaded from the MinERva Portal, managed by the Emilia-Romagna Region service [<a href="#B46-remotesensing-16-04066" class="html-bibr">46</a>].</p>
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<p>(<b>a</b>) Geological map of Carpi–Correggio–Soliera subsidence hotspot, overlapped onto Google satellite imagery; (<b>b</b>) Comparison between piezometric level variations in MO10-01 ARPAE’s monitoring well and a deformation time series of a contiguous PS–DS point; (<b>c</b>) Deformation velocities observed within each lithology, expressed in [mm/year]. Geological layers used were downloaded from the MinERva Portal, managed by the Emilia-Romagna Region service [<a href="#B46-remotesensing-16-04066" class="html-bibr">46</a>].</p>
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<p>Example of a time series classified as “Bilinear” by PS-Time automatic classification algorithm, in the southern area of Soliera.</p>
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<p>Time series of one of the PS–DS points scored positively for Bo2–IC2 seasonal component.</p>
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<p>Acceleration variations vs. buffer distances from Angela Angelina reinjection well.</p>
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15 pages, 16699 KiB  
Article
Spatiotemporal Relationship Between Land Subsidence and Ecological Environmental Quality in Shenfu Mining Area, Loess Plateau, China
by Shuaizhi Kang, Xia Jia, Yonghua Zhao, Yong Ao and Chaoqun Ma
ISPRS Int. J. Geo-Inf. 2024, 13(11), 390; https://doi.org/10.3390/ijgi13110390 - 31 Oct 2024
Viewed by 737
Abstract
The exploitation of coal resources has caused problems such as ground deformation, affecting the ecological environment. Spatiotemporal varying characteristics between land subsidence and ecological environmental quality (EEQ) are an important research hotspot. Using the SBAS-InSAR method, 64 Sentinel-1 images were utilized to monitor [...] Read more.
The exploitation of coal resources has caused problems such as ground deformation, affecting the ecological environment. Spatiotemporal varying characteristics between land subsidence and ecological environmental quality (EEQ) are an important research hotspot. Using the SBAS-InSAR method, 64 Sentinel-1 images were utilized to monitor land subsidence in the Shenfu mining area, one of China’s largest coal source regions. And the remote sensing ecological index (RSEI) was used to monitor and evaluate EEQ of the Shenfu mining area. Global and local spatial autocorrelation methods were used to assess the spatial aggregation degree and change patterns over time. Spatial Econometric Models were employed to explore the impacts of land subsidence on EEQ. The results showed the following: (1) The average RSEI values in the Shenfu mining area were 0.531, 0.488, and 0.523 in 2016, 2018, and 2020, respectively; there was a slight downward trend in EEQ. The permanent scatter (PS) point deformation rate ranged from −353.40 mm/year to +246.24 mm/year, with average deformation rates of 0.1642, 0.2181, and 0.2490 mm/year, respectively. (2) There was a significant correlation and spatial agglomeration effect between land surface subsidence and EEQ. Low–high, high–low, and low–low clusters were the main types of relationships, indicating that land subsidence primarily has a negative spatial impact on the ecological environment. (3) The relationship between land subsidence and EEQ varied spatially in the Shenfu mining area at 500 × 500 grid units. This research can provide scientific guidance for disaster prevention and sustainable development in mining areas by considering long-term differences in ecological environmental quality and its correlation with land subsidence. Full article
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<p>Location and DEM of the study area.</p>
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<p>The framework of this study.</p>
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<p>The spatiotemporal baselines (<b>a</b>) 2016; (<b>b</b>) 2018; (<b>c</b>) 2020.</p>
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<p>Spatial distribution characteristics of land subsidence (2016–2020) in Shenfu mining area.</p>
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<p>Spatial distribution of RSEI (2016–2020) in Shenfu mining area.</p>
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<p>Local indicator of spatial autocorrelation for land subsidence (2016–2020) in Shenfu mining area.</p>
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<p>Local indicator of spatial autocorrelation for EEQ (2016–2020) in Shenfu mining area.</p>
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<p>Bivariate local indicators of spatial associations between land subsidence and EEQ (2016–2020) in Shenfu mining area.</p>
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<p>Land-use map (2016–2020) in Shenfu mining area.</p>
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<p>Average temperature and precipitation (2016–2020) in Shenfu mining area.</p>
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22 pages, 64606 KiB  
Article
Spatial Variations and Regulating Processes of Groundwater Geochemistry in an Urbanized Valley Basin on Tibetan Plateau
by Wanping Wang, Shilong Zhang, Shengbin Wang, Chumeng Zhang, Guoqiang Zhang, Jie Wang, Liwei Wang, Hongjie Yang, Wenxu Hu, Yuqing Zhang, Ning Wang and Yong Xiao
Appl. Sci. 2024, 14(21), 9804; https://doi.org/10.3390/app14219804 - 27 Oct 2024
Viewed by 677
Abstract
Groundwater resource is crucial for the development of agriculture and urban communities in valley basins of arid and semiarid regions. This research investigated the groundwater chemistry of a typical urbanized valley basin on the Tibetan Plateau to understand the hydrochemical status, quality, and [...] Read more.
Groundwater resource is crucial for the development of agriculture and urban communities in valley basins of arid and semiarid regions. This research investigated the groundwater chemistry of a typical urbanized valley basin on the Tibetan Plateau to understand the hydrochemical status, quality, and controlling mechanisms of groundwater in arid urbanized valley basins. The results show groundwater is predominantly fresh and slightly alkaline across the basin, with approximately 54.17% of HCO3-Ca type. About 12.5% and 33.33% of sampled groundwaters are with the hydrochemical facies of Cl-Mg·Ca type and Cl-Na type, respectively. Groundwater is found with the maximum TDS, NO3, NO2, and F content of 3066 mg/L, 69.33 mg/L, 0.04 mg/L, and 3.12 mg/L, respectively. Groundwater quality is suitable for domestic usage at all sampling sites based on EWQI assessment but should avoid direct drinking at some sporadic sites in the urban area. The exceeding nitrogen and fluoride contaminants would pose potential health hazards to local residents, but high risks only existed for infants. Both minors and adults are at medium risk of these exceedingly toxic contaminants. Groundwater quality of predominant sites in the basin is suitable for long-term irrigation according to the single indicator of EC, SAR, %Na, RSC, KR, PI, and PS and integrated irrigation quality assessment of USSL, Wilcox, and Doneen diagram assessment. But sodium hazard, alkalinity hazard, and permeability problem should be a concern in the middle-lower stream areas. Groundwater chemistry in the basin is predominantly governed by water-rock interaction (silicate dissolution) across the basin in natural and sporadically by evaporation. Human activities have posed disturbances to groundwater chemistry and inputted nitrogen, fluoride, and salinity into groundwater. The elevated nitrogen contaminants in groundwater are from both agricultural activities and municipal sewage. While the elevated fluoride and salinity in groundwater are only associated with municipal sewage. It is imperative to address the potential anthropogenic contaminants to safeguard groundwater resources from the adverse external impacts of human settlements within these urbanized valley basins. Full article
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<p>Location of the (<b>a</b>) Huangshui Basin, (<b>b</b>) the Beichuan River basin, and (<b>c</b>) groundwater sampling site within the basin.</p>
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<p>Spatial distribution of (<b>a</b>) TDS, (<b>b</b>) NO<sub>3</sub><sup>−</sup>, and (<b>c</b>) F<sup>−</sup> content in groundwater within the basin.</p>
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<p>Piper diagram of groundwater regarding different TDS in the Beichuan River watershed.</p>
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<p>(<b>a</b>) Scatterplot of EWQI versus TDS, and (<b>b</b>) the spatial interpolation graph of EWQI of groundwater within the basin.</p>
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<p>Boxplots of health risks among various population groups within the basin.</p>
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<p>Spatial potential health risks to (<b>a</b>) infants, (<b>b</b>) children, (<b>c</b>) adult females, and (<b>d</b>) adult males in the basin.</p>
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<p>The irrigation water quality demonstrated by (<b>a</b>) USSL diagram, (<b>b</b>) Wilcox diagram, and (<b>c</b>) Doneen diagram.</p>
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<p>The Gibbs diagram of (<b>a</b>) TDS versus Na<sup>+</sup>/(Na<sup>+</sup>+Ca<sup>2+</sup>) and (<b>b</b>) TDS versus Cl<sup>−</sup>/(Cl<sup>+</sup>+HCO<sub>3</sub><sup>−</sup>) of groundwater within the basin.</p>
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<p>The endmember diagrams of (<b>a</b>) (Mg<sup>2+</sup>/Na<sup>+</sup>) versus (Ca<sup>2+</sup>/Na<sup>+</sup>) and (<b>b</b>) (HCO<sub>3</sub><sup>−</sup>/Na<sup>+</sup>) versus (Ca<sup>2+</sup>/Na<sup>+</sup>) of groundwater within the basin.</p>
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<p>Spearman correlation analysis of groundwater chemical parameters.</p>
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<p>Relationship Diagram of (NO<sub>3</sub><sup>−</sup>/Na<sup>+</sup>) versus (Cl<sup>−</sup>/Na<sup>+</sup>) ratios of groundwater within the basin.</p>
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21 pages, 10071 KiB  
Article
Deformation Monitoring and Analysis of Baige Landslide (China) Based on the Fusion Monitoring of Multi-Orbit Time-Series InSAR Technology
by Kai Ye, Zhe Wang, Ting Wang, Ying Luo, Yiming Chen, Jiaqian Zhang and Jialun Cai
Sensors 2024, 24(20), 6760; https://doi.org/10.3390/s24206760 - 21 Oct 2024
Viewed by 1108
Abstract
Due to the limitations inherent in SAR satellite imaging modes, utilizing time-series InSAR technology to process single-orbit satellite image data typically only yields one-dimensional deformation information along the LOS direction. This constraint impedes a comprehensive representation of the true surface deformation of landslides. [...] Read more.
Due to the limitations inherent in SAR satellite imaging modes, utilizing time-series InSAR technology to process single-orbit satellite image data typically only yields one-dimensional deformation information along the LOS direction. This constraint impedes a comprehensive representation of the true surface deformation of landslides. Consequently, in this paper, after the SBAS-InSAR and PS-InSAR processing of the 30-view ascending and 30-view descending orbit images of the Sentinel-1A satellite, based on the imaging geometric relationship of the SAR satellite, we propose a novel computational method of fusing ascending and descending orbital LOS-direction time-series deformation to extract the landslide’s downslope direction deformation of landslides. By applying this method to Baige landslide monitoring and integrating it with an improved tangential angle warning criterion, we classified the landslide’s trailing edge into a high-speed, a uniform-speed, and a low-speed deformation region, with deformation magnitudes of 7~8 cm, 5~7 cm, and 3~4 cm, respectively. A comparative analysis with measured data for landslide deformation monitoring revealed that the average root mean square error between the fused landslide’s downslope direction deformation and the measured data was a mere 3.62 mm. This represents a reduction of 56.9% and 57.5% in the average root mean square error compared to the single ascending and descending orbit LOS-direction time-series deformations, respectively, indicating higher monitoring accuracy. Finally, based on the analysis of landslide deformation and its inducing factors derived from the calculated time-series deformation results, it was determined that the precipitation, lithology of the strata, and ongoing geological activity are significant contributors to the sliding of the Baige land-slide. This method offers more comprehensive and accurate surface deformation information for dynamic landslide monitoring, aiding relevant departments in landslide surveillance and management, and providing technical recommendations for the fusion of multi-orbital satellite LOS-direction deformations to accurately reconstruct the true surface deformation of landslides. Full article
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<p>InSAR technology imaging mode.</p>
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<p>Main body of the landslide and research area, where (<b>a</b>) is the main body of the landslide and the extent of the study area and (<b>b</b>) is the location of the Baige landslide.</p>
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<p>The specific imaging modes of satellites in ascending and descending orbits. Where (<b>a</b>) is the ascending orbit satellite imaging mode and (<b>b</b>) is the descending orbit satellite imaging mode.</p>
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<p>Fusion extraction flow chart.</p>
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<p>LOS direction deformation transformation model.</p>
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<p>Spatial and temporal baseline maps, where (<b>a</b>,<b>b</b>) are time–position plots of the descending and ascending orbit images; (<b>c</b>,<b>d</b>) are time–baseline plots of the descending and ascending orbit images. (These diagrams were drawn using ENVI-SARscape5.6.2 software).</p>
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<p>Time-series deformation results obtained from the SBAS-InSAR. Where (<b>a</b>) is for the descending orbit dataset and (<b>b</b>) is for the ascending orbit dataset.</p>
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<p>Location of feature points on the trailing edge of the Baige landslide: (<b>a</b>) is the location of the points in the satellite map and (<b>b</b>) is the location of the points in the descending orbit time-series deformation result.</p>
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<p>Time-series deformation results for each monitoring point. Where (<b>a</b>–<b>i</b>) are the time-series deformations of the corresponding monitoring points (A–I). The blue line is the descending orbit LOS direction time-series deformation, the orange line is the ascending orbit LOS direction time-series deformation, and the green line is the landslide’s downslope direction time-series deformation.</p>
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<p>Delineation of landslide areas.</p>
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<p>Distribution of errors between each time-series deformation result dataset and measured data, where (B), (F) and (H) represent the deformation information of points B, F and H respectively, M-D<sub>S</sub> in green is the error between the measured data and the extracted landslide’s downslope direction deformation, M-D<sub>A</sub> in red is the error between the measured data and the ascending time-series deformation, M-D<sub>D</sub> in blue is the error between the measured data and the descending time-series deformation, and the red dashed line is the time when the first landslide occurred.</p>
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<p>Relationship between tangential angle and monthly mean deformation and rainfall in different regions around the landslide trailing edge. Where (<b>a</b>–<b>c</b>) are the specific information of Area D1, D2, and D3, respectively, and (<b>d</b>) is the location map of the areas.</p>
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<p>Results from PS-InSAR processing of descending orbit images: (<b>a</b>,<b>b</b>) are the time-position plot and time-baseline plot of the descending orbit images; (<b>c</b>) is a permanent scatterer point position map of the descending orbit images.</p>
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<p>Results from PS-InSAR processing of ascending orbit images: (<b>a</b>,<b>b</b>) are the time-position plot and time-baseline plot of the ascending orbit images; (<b>c</b>) is a permanent scatterer point position map of the ascending orbit images.</p>
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<p>Stratigraphic lithology and topographic map of the Baige landslide.</p>
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21 pages, 23010 KiB  
Article
Three-Dimensional Reconstruction of Partially Coherent Scatterers Using Iterative Sub-Network Generation Method
by Xiantao Wang, Zhen Dong, Youjun Wang, Xing Chen and Anxi Yu
Remote Sens. 2024, 16(19), 3707; https://doi.org/10.3390/rs16193707 - 5 Oct 2024
Viewed by 613
Abstract
Synthetic aperture radar tomography (TomoSAR) has gained significant attention for three-dimensional (3D) imaging in urban environments. A notable limitation of traditional TomoSAR approaches is their primary focus on persistent scatterers (PSs), disregarding targets with temporal decorrelated characteristics. Temporal variations in coherence, especially in [...] Read more.
Synthetic aperture radar tomography (TomoSAR) has gained significant attention for three-dimensional (3D) imaging in urban environments. A notable limitation of traditional TomoSAR approaches is their primary focus on persistent scatterers (PSs), disregarding targets with temporal decorrelated characteristics. Temporal variations in coherence, especially in urban areas due to the dense population of buildings and artificial structures, can lead to a reduction in detectable PSs and suboptimal 3D reconstruction performance. The concept of partially coherent scatterers (PCSs) has been proven effective by capturing the partial temporal coherence of targets across the entire time baseline. In this study, an novel approach based on an iterative sub-network generation method is introduced to leverage PCSs for enhanced 3D reconstruction in dynamic environments. We propose a coherence constraint iterative variance analysis approach to determine the optimal temporal baseline range that accurately reflects the interferometric coherence of PCSs. Utilizing the selected PCSs, a 3D imaging technique that incorporates the iterative generation of sub-networks into the SAR tomography process is developed. By employing the PS reference network as a foundation, we accurately invert PCSs through the iterative generation of local star-shaped networks, ensuring a comprehensive coverage of PCSs in study areas. The effectiveness of this method for the height estimation of PCSs is validated using the TerraSAR-X dataset. Compared with traditional PS-based TomoSAR, the proposed approach demonstrates that PCS-based elevation results complement those from PSs, significantly improving 3D reconstruction in evolving urban settings. Full article
(This article belongs to the Special Issue Synthetic Aperture Radar Interferometry Symposium 2024)
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<p>Three types of PCSs based on the division of coherence intervals. (<b>a</b>) Appearing-type PCS (APCS). (<b>b</b>) Disappearing-type PCS (DPCS). (<b>c</b>) Visiting-type PCS (VPCS). The red dashed lines represent the image index when coherence of PCS changes.</p>
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<p>Flowchart of PCS detection based on iterative ANOVA and coherent interval confirmation.</p>
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<p>Flowchart of the proposed height inversion of the PCSs iterative network generation method. The red dashed box represents the flowchart of the PCS detection method in <a href="#sec3dot1-remotesensing-16-03707" class="html-sec">Section 3.1</a>.</p>
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<p>Spatial–Temporal baseline distribution of the SAR images. The highlighted red circle represents the master image.</p>
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<p>SAR and optical image of the study area. (<b>a</b>) SAR average amplitude map. (<b>b</b>) PS distribution map. (<b>c</b>) Google optical image acquired in December 2015. (<b>d</b>) Google optical image acquired in January 2017.</p>
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<p>PCS distribution map.</p>
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<p>Distribution of different types of PCSs. (<b>a</b>) APCS. (<b>b</b>) DPCS. The color bar represents the step change positions.</p>
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<p>The distribution of PCSs in Areas A–D of <a href="#remotesensing-16-03707-f007" class="html-fig">Figure 7</a>, along with the corresponding SAR average amplitude image. (<b>a</b>) Area A. (<b>b</b>) Area B. (<b>c</b>) Area C. (<b>d</b>) Area D. The color bar in this figure is consistent with that in <a href="#remotesensing-16-03707-f007" class="html-fig">Figure 7</a>.</p>
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<p>Distribution of PS RN and height map of PSs. (<b>a</b>) Distribution of the PS RN. (<b>b</b>) Height map of PSs. The selected reference point with zero height is located on the left side of the road, indicated by a yellow pentagon star.</p>
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<p>Distribution of the PCS sub-network. (<b>a</b>) APCS sub-network. (<b>b</b>) DPCS sub-network. The yellow edges represent newly generated connections to the PCSs. The blue color indicates the PS RN while yellow color represents the subnetwork.</p>
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<p>The iterative generation process of the APCS sub-network, where (<b>a</b>–<b>h</b>) represents iteration number 1–8. Red points are PSs and APCSs, blue edges denote edges in the PS RN, and yellow edges indicate those in the newly generated sub-network.</p>
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<p>Height point cloud map of different types of PCS. (<b>a</b>) APCS. (<b>b</b>) DPCS.</p>
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<p>(<b>a</b>) Height point cloud map of PS. (<b>b</b>) Unified height point cloud map of PS and APCS. (<b>c</b>) Unified height point cloud map of PS and DPCS. The selected reference point with zero height is the same as in <a href="#remotesensing-16-03707-f010" class="html-fig">Figure 10</a>b.</p>
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22 pages, 17408 KiB  
Article
InSAR Integrated Machine Learning Approach for Landslide Susceptibility Mapping in California
by Divya Sekhar Vaka, Vishnuvardhan Reddy Yaragunda, Skevi Perdikou and Alexandra Papanicolaou
Remote Sens. 2024, 16(19), 3574; https://doi.org/10.3390/rs16193574 - 25 Sep 2024
Viewed by 1595
Abstract
Landslides pose significant threats to life and property, particularly in mountainous regions. To address this, this study develops a landslide susceptibility model integrating Earth Observation (EO) data, historical data, and Multi-Temporal Interferometric Synthetic Aperture Radar (MT-InSAR) ground movement results. The model categorizes areas [...] Read more.
Landslides pose significant threats to life and property, particularly in mountainous regions. To address this, this study develops a landslide susceptibility model integrating Earth Observation (EO) data, historical data, and Multi-Temporal Interferometric Synthetic Aperture Radar (MT-InSAR) ground movement results. The model categorizes areas into four susceptibility classes (from Class 1 to Class 4) using a multi-class classification approach. Results indicate that the Xtreme Gradient Boosting (XGB) model effectively predicts landslide susceptibility with area under the curve (AUC) values ranging from 0.93 to 0.97, with high accuracy of 0.89 and a balanced performance across different susceptibility classes. The integration of MT-InSAR data enhances the model’s ability to capture dynamic ground movement and improves landslide mapping. The landslide susceptibility map generated by the XGB model indicates high susceptibility along the Pacific coast. The optimal model was validated against 272 historical landslide occurrences, with predictions distributed as follows: 68 occurrences (25%) in Class 1, 142 occurrences (52%) in Class 2, 58 occurrences (21.5%) in Class 3, and 4 occurrences (1.5%) in Class 4. This study highlights the importance of considering temporal changes in environmental conditions such as precipitation, distance to streams, and changes in vegetation for accurate landslide susceptibility assessment. Full article
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<p>Study area map of the San Francisco region, featuring a United States Geological Survey (USGS) 3D Elevation Program (3DEP) Digital Elevation Model as the background. Historical landslide locations are denoted with red triangles (with 272 landslides falling within the study area AOI), and the extent of the Sentinel–-1 Synthetic Aperture Radar (SAR) image is outlined with a black polygon.</p>
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<p>The geological profile of the study area was obtained from the USGS website [<a href="#B43-remotesensing-16-03574" class="html-bibr">43</a>], based on a 1:250,000 scale digitized map. Major faults overlaid on the map are retrieved from the California State Geoportal (<a href="https://gis.data.ca.gov/" target="_blank">https://gis.data.ca.gov/</a>, accessed on 11 August 2023).</p>
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<p>Different landslide causative factors used as input to the machine learning model. (<b>A</b>) slope, (<b>B</b>) aspect, (<b>C</b>) curvature, (<b>D</b>) flow direction, (<b>E</b>) distance from a stream, (<b>F</b>) rainfall, (<b>G</b>) vegetation, (<b>H</b>) soil type, and (<b>I</b>) geology.</p>
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<p>Flowchart of the proposed method.</p>
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<p>MT-InSAR line-of-sight mean velocity map indicating deformation rates in the San Francisco area, with Google Earth Imagery as the background. Displacement time series plots at selected locations, including Treasure Island (TI), San Francisco International Airport (SFO), Foster City (FC), and within Santa Clara Valley (SCV), are shown in <a href="#remotesensing-16-03574-f006" class="html-fig">Figure 6</a>. Additional displacement time series plots at historical landslide locations (from A to G) are shown in <a href="#remotesensing-16-03574-f007" class="html-fig">Figure 7</a>. The fault layer overlaid on the velocity map is retrieved from the California State Geoportal (<a href="https://gis.data.ca.gov/" target="_blank">https://gis.data.ca.gov/</a>, accessed on 11 August 2023).</p>
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<p>MT-InSAR displacement time series at (<b>a</b>) Treasure Island (TI), (<b>b</b>) San Francisco International Airport (SFO), (<b>c</b>) Foster City (FC), and (<b>d</b>,<b>e</b>) within Santa Clara Valley (SCV).</p>
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<p>(<b>a</b>–<b>g</b>) MT-InSAR displacement time series at historical landslide locations denoted with letters A to G in <a href="#remotesensing-16-03574-f005" class="html-fig">Figure 5</a>. The area corresponding to the high deformation rate in (<b>f</b>) is shown in <a href="#remotesensing-16-03574-f008" class="html-fig">Figure 8</a>.</p>
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<p>An area indicating a high deformation rate (subsidence) in the MT-InSAR analysis is observed near historical landslide locations (marked by red triangles) adjacent to the Green Valley Fault (GVF).</p>
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<p>ROC curve and AUC values for each class (from Class 1 to Class 4) using RF and XGB models with original and oversampled data.</p>
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<p>Comparative metrics for RF and XGB models with original and oversampled data.</p>
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<p>Tree map showing the importance of factors used in this study.</p>
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<p>Landslide susceptibility map of the San Francisco area derived using the XGB model. The map classifies landslides into four categories, ranging from no susceptibility to high susceptibility. The historical landslide locations are overlaid on the susceptibility map.</p>
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35 pages, 2230 KiB  
Review
Navigating the Landscape of B Cell Mediated Immunity and Antibody Monitoring in SARS-CoV-2 Vaccine Efficacy: Tools, Strategies and Clinical Trial Insights
by Sophie O’Reilly, Joanne Byrne, Eoin R. Feeney, Patrick W. G. Mallon and Virginie Gautier
Vaccines 2024, 12(10), 1089; https://doi.org/10.3390/vaccines12101089 - 24 Sep 2024
Viewed by 1525
Abstract
Correlates of Protection (CoP) are biomarkers above a defined threshold that can replace clinical outcomes as primary endpoints, predicting vaccine effectiveness to support the approval of new vaccines or follow up studies. In the context of COVID-19 vaccination, CoPs can help address challenges [...] Read more.
Correlates of Protection (CoP) are biomarkers above a defined threshold that can replace clinical outcomes as primary endpoints, predicting vaccine effectiveness to support the approval of new vaccines or follow up studies. In the context of COVID-19 vaccination, CoPs can help address challenges such as demonstrating vaccine effectiveness in special populations, against emerging SARS-CoV-2 variants or determining the durability of vaccine-elicited immunity. While anti-spike IgG titres and viral neutralising capacity have been characterised as CoPs for COVID-19 vaccination, the contribution of other components of the humoral immune response to immediate and long-term protective immunity is less well characterised. This review examines the evidence supporting the use of CoPs in COVID-19 clinical vaccine trials, and how they can be used to define a protective threshold of immunity. It also highlights alternative humoral immune biomarkers, including Fc effector function, mucosal immunity, and the generation of long-lived plasma and memory B cells and discuss how these can be applied to clinical studies and the tools available to study them. Full article
(This article belongs to the Special Issue Immune Effectiveness of COVID-19 Vaccines)
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<p>Viral Neutralisation Assays. (<b>A</b>) SARS-CoV-2 virion with the four structural proteins: spike, nucleoprotein, envelope and membrane and details of the spike S1 and S2 subunits and the Receptor Binding Domain (RBD). SARS-CoV-2 pseudovirus expressing spike proteins and harbouring a GFP reporter gene. (<b>A1</b>) Plasma or serum, containing a variety of Ig is serially diluted and mixed with live virus or pseudovirus facilitating the interactions between Ig and spike. Panel (<b>B</b>). (<b>B2</b>) Virus/Ig mixture is added to a monolayer of cells. Spike mediates viral entry through interaction between RBD and the ACE2 receptor on the host cell. Spike targeted by neutralising antibodies fail to interact with ACE2 and block viral entry. (<b>B3</b>) Within hours, viral replication takes place, leading to formation and release of new virions, ultimately resulting in cell death. Alternatively cells infected with SARS-CoV-2 pseudovirus produce GFP or luciferase encoded by a reporter gene. (<b>B4</b>) For live-virus assays, infection can be quantified through observed cytopathic effects (CPE) such as visual counting of plaques or scoring CPE in each well using a light microscope (TCID50). Immunostaining facilitates the detection of viral proteins. Micro-foci can be quantified using spot-readers, or infected cells can be quantified using flow cytometry. RT-qPCR quantifies cell-associated viral RNA or viral load. Spectrophotometry quantifies fluorescence or luminescence in cells infected with SARS-CoV-2 pseudovirus. Panel (<b>C</b>). (<b>C5</b>) The reciprocal of the plasma dilution that reduces infection rate by 50% (NT50) is determined by normalising the infection rate measured for each dilution to positive (no plasma) and negative (no virus) control wells. (<b>C6</b>) The NT50 values can then be compared between cohorts e.g., with different vaccine strategies or against SARS-CoV-2 variants to monitor immune escape. NT50 values can also be correlated with other humoral immune biomarkers such as specific IgG titres. Created with <a href="http://BioRender.com" target="_blank">BioRender.com</a> (accessed on 19 September 2024).</p>
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<p>Fc Effector Function Assays. (<b>A</b>) Assays require plasma or serum, target cells or beads expressing or coated with SARS-CoV-2 antigens (spike), and effectors including complement proteins, primary innate immune cells or genetically modified cell lines with luciferase (luc) reporter gene. (<b>B1</b>) Target cells or beads are incubated with Ig facilitating the binding of specific Ig Fab-domains to SARS-CoV-2 antigens on the cell surface. (<b>B2</b>) Antigen-bound Ig can bind FcRs on effector cells or bind soluble complement via their Fc domains. (<b>B3</b>) This results in (<b>a</b>) elimination of target cells through Antibody-Dependent Cellular Phagocytosis (ADCP) or Antibody-Dependent Cellular Cytotoxicity (ADCC) or Antibody-Dependent Complement Deposition (ADCD), (<b>b</b>) uptake of target beads by ADCP or (<b>c</b>) expression of luciferase by effector cells. (<b>B4</b>) Flow cytometry can be used to measure (<b>a</b>) reduction of fluorescent target cells, or (<b>b</b>) uptake of fluorescent beads by effector cells. (<b>c</b>) Luminometry or spectrophotometry can be used to measure expression of luciferase by effector cells. Created with <a href="http://Biorender.com" target="_blank">Biorender.com</a> (accessed on 19 September 2024).</p>
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<p>B cell Assays. (<b>A</b>) Peripheral Blood Mononuclear Cells (PBMCs) and fluorescently labelled SARS-CoV-2 antigens (spike). (<b>A1</b>) SARS-CoV-2 antigens are incubated with PBMCs and are captured by antigen-specific B cells. (<b>A2</b>) B cells are stained with fluorescent antibodies against B cell markers e.g., CD19 (<b>A3</b>,<b>A4</b>) B cells and B cell subsets can be identified via flow cytometry using B cell markers. (<b>A5</b>) B cells bound to the antigen of interest can be identified using the fluorescently-tagged antigens. (<b>A6</b>) B cell isotypes can be identified using fluorescently-tagged antibodies against specific Ig. Panel (<b>B</b>). (<b>B1</b>) Antibody Secreting Cells (ASC) can be isolated directly from PBMCs or memory B cells can be stimulated for 72 h to trigger differentiation into ASC. (<b>B2</b>) ASC are added to a plate coated with SARS-CoV-2 antigen (spike). (<b>B3</b>) Ig are continuously released by ASC. Spike-specific Ig bind surface-bound antigen local to the ASC. (<b>B4</b>) Cells and unbound Ig are removed by washing. Biotinylated anti-human IgG antibodies are added to detect specific-IgG bound to the surface antigen. (<b>B5</b>) Enzyme-labelled streptavidin is added to the well and is captured by the biotinylated detection antibodies. (<b>B6</b>) A substrate is added which forms a coloured insoluble precipitate when catalysed by the antibody-bound enzyme. Each antigen-specific ASC forms a single spot which can be read with an automated spot reader. Created with <a href="http://BioRender.com" target="_blank">BioRender.com</a> (accessed on 19 September 2024).</p>
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10 pages, 224 KiB  
Article
Methotrexate and Tumor Necrosis Factor Inhibitors Independently Decrease Neutralizing Antibodies after SARS-CoV-2 Vaccination: Updated Results from the SUCCEED Study
by Carol A Hitchon, Dawn M. E. Bowdish, Gilles Boire, Paul R. Fortin, Louis Flamand, Vinod Chandran, Roya M. Dayam, Anne-Claude Gingras, Catherine M. Card, Inés Colmegna, Maggie J. Larché, Gilaad G. Kaplan, Luck Lukusa, Jennifer L.F. Lee, Sasha Bernatsky and on behalf of the SUCCEED Investigative Team
Vaccines 2024, 12(9), 1061; https://doi.org/10.3390/vaccines12091061 - 17 Sep 2024
Viewed by 1204
Abstract
Objective: SARS-CoV-2 remains the third most common cause of death in North America. We studied the effects of methotrexate and tumor necrosis factor inhibitor (TNFi) on neutralization responses after COVID-19 vaccination in immune-mediated inflammatory disease (IMID). Methods: Prospective data and sera of adults [...] Read more.
Objective: SARS-CoV-2 remains the third most common cause of death in North America. We studied the effects of methotrexate and tumor necrosis factor inhibitor (TNFi) on neutralization responses after COVID-19 vaccination in immune-mediated inflammatory disease (IMID). Methods: Prospective data and sera of adults with inflammatory bowel disease (IBD), rheumatoid arthritis (RA), spondyloarthritis (SpA), psoriatic arthritis (PsA), and systemic lupus (SLE) were collected at six academic centers in Alberta, Manitoba, Ontario, and Quebec between 2022 and 2023. Sera from two time points were evaluated for each subject. Neutralization studies were divided between five laboratories, and each lab’s results were analyzed separately using multivariate generalized logit models (ordinal outcomes: absent, low, medium, and high neutralization). Odds ratios (ORs) for the effects of methotrexate and TNFi were adjusted for demographics, IMID, other biologics and immunosuppressives, prednisone, COVID-19 vaccinations (number/type), and infections in the 6 months prior to sampling. The adjusted ORs for methotrexate and TNFi were then pooled in random-effects meta-analyses (separately for the ancestral strains and the Omicron BA1 and BA5 strains). Results: Of 479 individuals (958 samples), 292 (61%) were IBD, 141 (29.4%) were RA, and the remainder were PsA, SpA, and SLE. The mean age was 57 (62.2% female). For both the individual labs and the meta-analyses, the adjusted ORs suggested independent negative effects of TNFi and methotrexate on neutralization. The meta-analysis adjusted ORs for TNFi were 0.56 (95% confidence interval (CI) 0.39, 0.81) for the ancestral strain and 0.56 (95% CI 0.39, 0.81) for BA5. The meta-analysis adjusted OR for methotrexate was 0.39 (95% CI 0.19, 0.76) for BA1. Conclusions: SARS-CoV-2 neutralization in vaccinated IMID was diminished independently by TNFi and methotrexate. As SARS-CoV-2 circulation continues, ongoing vigilance regarding optimized vaccination is required. Full article
(This article belongs to the Section Vaccine Efficacy and Safety)
11 pages, 246 KiB  
Article
How Safe Are COVID-19 Vaccines in Individuals with Immune-Mediated Inflammatory Diseases? The SUCCEED Study
by Olga Tsyruk, Gilaad G. Kaplan, Paul R. Fortin, Carol A Hitchon, Vinod Chandran, Maggie J. Larché, Antonio Avina-Zubieta, Gilles Boire, Ines Colmegna, Diane Lacaille, Nadine Lalonde, Laurie Proulx, Dawn P. Richards, Natalie Boivin, Christopher DeBow, Lucy Kovalova-Wood, Deborah Paleczny, Linda Wilhelm, Luck Lukusa, Daniel Pereira, Jennifer LF. Lee, Sasha Bernatsky and on behalf of the SUCCEED Investigative Teamadd Show full author list remove Hide full author list
Vaccines 2024, 12(9), 1027; https://doi.org/10.3390/vaccines12091027 - 8 Sep 2024
Viewed by 1323
Abstract
We were tasked by Canada’s COVID-19 Immunity Task Force to describe severe adverse events (SAEs) associated with emergency department (ED) visits and/or hospitalizations in individuals with immune-mediated inflammatory diseases (IMIDs). At eight Canadian centres, data were collected from adults with rheumatoid arthritis (RA), [...] Read more.
We were tasked by Canada’s COVID-19 Immunity Task Force to describe severe adverse events (SAEs) associated with emergency department (ED) visits and/or hospitalizations in individuals with immune-mediated inflammatory diseases (IMIDs). At eight Canadian centres, data were collected from adults with rheumatoid arthritis (RA), axial spondyloarthritis (AxS), systemic lupus (SLE), psoriatic arthritis (PsA), and inflammatory bowel disease (IBD). We administered questionnaires, analyzing SAEs experienced within 31 days following SARS-CoV-2 vaccination. About two-thirds (63%) of 1556 participants were female; the mean age was 52.5 years. The BNT162b2 (Pfizer) vaccine was the most common, with mRNA-1273 (Moderna) being second. A total of 49% of participants had IBD, 27.4% had RA, 14.3% had PsA, 5.3% had SpA, and 4% had SLE. Twelve (0.77% of 1556 participants) SAEs leading to an ED visit or hospitalization were self-reported, occurring in 11 participants. SAEs included six (0.39% of 1556 participants) ED visits (including one due to Bell’s Palsy 31 days after first vaccination) and six (0.39% of 1556 participants) hospitalizations (including one due to Guillain-Barré syndrome 15 days after the first vaccination). Two SAEs included pericarditis, one involved SLE (considered a serious disease flare), and one involved RA. Thus, in the 31 days after SARS-CoV-2 vaccination in our IMID sample, very few serious adverse events occurred. As SARS-CoV2 continues to be a common cause of death, our findings may help optimize vaccination acceptance. Full article
(This article belongs to the Section Vaccine Efficacy and Safety)
14 pages, 2389 KiB  
Article
MoMo30 Binds to SARS-CoV-2 Spike Variants and Blocks Infection by SARS-CoV-2 Pseudovirus
by Kenya DeBarros, Mahfuz Khan, Morgan Coleman, Vincent C. Bond, Virginia Floyd, Erick Gbodossou, Amad Diop, Lauren R. H. Krumpe, Barry R. O’Keefe and Michael D. Powell
Viruses 2024, 16(9), 1433; https://doi.org/10.3390/v16091433 - 7 Sep 2024
Viewed by 1344
Abstract
MoMo30 is an antiviral protein isolated from aqueous extracts of Momordica balsamina L. (Senegalese bitter melon). Previously, we demonstrated MoMo30’s antiviral activity against HIV-1. Here, we explore whether MoMo30 has antiviral activity against the COVID-19 virus, SARS-CoV-2. MLV particles pseudotyped with the SARS-CoV-2 [...] Read more.
MoMo30 is an antiviral protein isolated from aqueous extracts of Momordica balsamina L. (Senegalese bitter melon). Previously, we demonstrated MoMo30’s antiviral activity against HIV-1. Here, we explore whether MoMo30 has antiviral activity against the COVID-19 virus, SARS-CoV-2. MLV particles pseudotyped with the SARS-CoV-2 Spike glycoprotein and a Luciferase reporter gene (SARS2-PsV) were developed from a three-way co-transfection of HEK293-T17 cells. MoMo30’s inhibition of SARS2-PsV infection was measured using a luciferase assay and its cytotoxicity using an XTT assay. Additionally, MoMo30’s interactions with the variants and domains of Spike were determined by ELISA. We show that MoMo30 inhibits SARS2-PsV infection. We also report evidence of the direct interaction of MoMo30 and SARS-CoV-2 Spike from WH-1, Alpha, Delta, and Omicron variants. Furthermore, MoMo30 interacts with both the S1 and S2 domains of Spike but not the receptor binding domain (RBD), suggesting that MoMo30 inhibits SARS-CoV-2 infection by inhibiting fusion of the virus and the host cell via interactions with Spike. Full article
(This article belongs to the Section Coronaviruses)
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<p>(<b>A</b>) SARS2-PsV assay. This assay was conducted in triplicate, generating a dose–response curve measuring the viral inhibition of the crude (●) and ammonium sulfate precipitated extracts (<span style="color:#DE1077">■</span><span style="color:#00247D">)</span> of MoMo30 against SARS2-PsV. (<b>B</b>) XTT Cytotoxicity assay. The assay was conducted in tandem with the PsV assay with the percent cell viability compared with non-treated controls for each concentration shown. The (*) indicate statistically significant difference (<span class="html-italic">p</span> &lt; 0.05) in cytotoxicity between treated on non-treated controls. (<b>C</b>) SARS2-PsV assay of <span class="html-italic">M. balsamina</span> tannins. This is a dose–response curve measuring the viral inhibition of the isolated tannins against SARS2-PsV. Treatment concentrations range from 0 to 17.61 μg/mL. (<b>D</b>) XTT Cytotoxicity assay of <span class="html-italic">M. balsamina</span> tannins. Concentrations of tannins are in the same dose range. The (*) indicate statistically significant difference (<span class="html-italic">p</span> &lt; 0.05) in cytotoxicity between treated on non-treated controls. The assays were conducted in triplicate. The mean and SD are shown.</p>
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<p>Spike Variant ELISA. MoMo30 binds to the SARS-CoV-2 Spike glycoprotein Wu Han-1, Alpha, Delta, and Omicron BA.1 Spike variants. The assays were performed in triplicate. The mean and SD are shown.</p>
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<p>MoMo30 binds to full-length SARS-CoV-2 spike glycoprotein, the isolated S1 and S2 domains of Wu Han Spike, but not to the isolated receptor binding domain (RBD). The assays were done in triplicate. The mean and SD are shown.</p>
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<p>S1 Conformation Inhibition Hypothesis. The Spike protein consists of the S1 (light green) and S2 (dark green) domains. The RBD (yellow) adopts an “up” conformation when binding to the ACE2 receptor (pink) to allow viral attachment to the host cell. MoMo30 (blue) binds to the S1 domain such that the RBD is stuck in the “down” conformation and cannot attach to ACE2.</p>
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<p>S2 Conformation Inhibition Hypothesis. (<b>A</b>) Spike protein is cleaved by host cell proteases (represented with a pair of scissors) and releases the S1 domain. (<b>B</b>) MoMo30 binds the S2 domain and inhibits the necessary conformation changes in the S2 for fusion to occur.</p>
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<p>Protease Inhibition Hypothesis. (<b>A</b>) Spike protein is cleaved by host cell proteases along the boundary between the S1 and S2 domains. The release of the S1 domain exposes the fusion peptide within the S2. (<b>B</b>) MoMo30 blocks the cleavage by the proteases.</p>
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