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Search Results (3)

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Keywords = Neverland

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20 pages, 3102 KiB  
Article
Identification and Evolution Analysis of the Genes Involved in the 20-Hydroxyecdysone Metabolism in the Mud Crab, Scylla paramamosain: A Preliminary Study
by Xin Jin, Lingbo Ma, Fengying Zhang, Linzi Zhang, Jinju Yin, Wei Wang and Ming Zhao
Genes 2024, 15(12), 1586; https://doi.org/10.3390/genes15121586 - 10 Dec 2024
Viewed by 696
Abstract
Background: 20-Hydroxyecdysone (20E) is the most ubiquitous ecdysteroid (Ecd) and plays critical roles during the life cycle of arthropods. To elucidate the metabolism pathway of 20E in the economically important species, Scylla paramamosain, we conducted a comprehensive exploration of the genes involved [...] Read more.
Background: 20-Hydroxyecdysone (20E) is the most ubiquitous ecdysteroid (Ecd) and plays critical roles during the life cycle of arthropods. To elucidate the metabolism pathway of 20E in the economically important species, Scylla paramamosain, we conducted a comprehensive exploration of the genes involved in the 20E metabolism pathway. Methods: A comprehensive exploration of genes involved in the 20E metabolism pathway was conducted, including gene annotation, local blast using the Drosophila ortholog as query, and TreeFam ortholog genes identification. Bioinformatics and expression profiling of the identified genes were performed to assess their roles in the 20E metabolism of green mud crabs. Results: This experiment indicated that, except for CYP306a1 and CYP314a1, all other ortholog genes involved in the Drosophila 20E metabolism can be found in the mud crab, suggesting that the function of these two genes might be replaced by other CYP genes or the “active” Ecd in mud crabs was not the 20E. All genes had the typical features of each gene family, clustered with the specific clade in the phylogenetic trees. In addition, all the identified genes had the highest expression level in the Y-organ, and sex-biased gene expression was observed in these genes. Conclusions: This study provided some valuable insights into the metabolism and diversity of ecdysteroids in crustaceans. Full article
(This article belongs to the Section Bioinformatics)
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Figure 1

Figure 1
<p>Tertiary structural modelling of 20E metabolism proteins.</p> Full article ">Figure 2
<p>Multiple sequence alignment analysis. Sequences with 100% similarity are highlighted with a black background, sequences with over 75% similarity are highlighted with a dark gray background, and sequences with over 50% similarity are highlighted with a light gray background. (<b>A</b>) Multiplex protein sequences alignment of Nvds. (<b>B</b>) Multiplex protein sequences alignment of CYP315a1. (<b>C</b>) Multiplex protein sequences alignment of CYP307a1. (<b>D</b>) Multiplex protein sequences alignment of CYP302a1. (<b>E</b>) Multiplex protein sequences alignment of CYP18a1.</p> Full article ">Figure 2 Cont.
<p>Multiple sequence alignment analysis. Sequences with 100% similarity are highlighted with a black background, sequences with over 75% similarity are highlighted with a dark gray background, and sequences with over 50% similarity are highlighted with a light gray background. (<b>A</b>) Multiplex protein sequences alignment of Nvds. (<b>B</b>) Multiplex protein sequences alignment of CYP315a1. (<b>C</b>) Multiplex protein sequences alignment of CYP307a1. (<b>D</b>) Multiplex protein sequences alignment of CYP302a1. (<b>E</b>) Multiplex protein sequences alignment of CYP18a1.</p> Full article ">Figure 2 Cont.
<p>Multiple sequence alignment analysis. Sequences with 100% similarity are highlighted with a black background, sequences with over 75% similarity are highlighted with a dark gray background, and sequences with over 50% similarity are highlighted with a light gray background. (<b>A</b>) Multiplex protein sequences alignment of Nvds. (<b>B</b>) Multiplex protein sequences alignment of CYP315a1. (<b>C</b>) Multiplex protein sequences alignment of CYP307a1. (<b>D</b>) Multiplex protein sequences alignment of CYP302a1. (<b>E</b>) Multiplex protein sequences alignment of CYP18a1.</p> Full article ">Figure 2 Cont.
<p>Multiple sequence alignment analysis. Sequences with 100% similarity are highlighted with a black background, sequences with over 75% similarity are highlighted with a dark gray background, and sequences with over 50% similarity are highlighted with a light gray background. (<b>A</b>) Multiplex protein sequences alignment of Nvds. (<b>B</b>) Multiplex protein sequences alignment of CYP315a1. (<b>C</b>) Multiplex protein sequences alignment of CYP307a1. (<b>D</b>) Multiplex protein sequences alignment of CYP302a1. (<b>E</b>) Multiplex protein sequences alignment of CYP18a1.</p> Full article ">Figure 2 Cont.
<p>Multiple sequence alignment analysis. Sequences with 100% similarity are highlighted with a black background, sequences with over 75% similarity are highlighted with a dark gray background, and sequences with over 50% similarity are highlighted with a light gray background. (<b>A</b>) Multiplex protein sequences alignment of Nvds. (<b>B</b>) Multiplex protein sequences alignment of CYP315a1. (<b>C</b>) Multiplex protein sequences alignment of CYP307a1. (<b>D</b>) Multiplex protein sequences alignment of CYP302a1. (<b>E</b>) Multiplex protein sequences alignment of CYP18a1.</p> Full article ">Figure 3
<p>Evolutionary tree. (<b>A</b>) The phylogenetic tree of Neverland in different species. The tree is constructed using the Neighbor-Joining (NJ) method. The size of the circle on the branch represents the bootstrap support rate. (<b>B</b>) The phylogenetic tree of P450 family genes involved in 20E metabolism. The trees is constructed using the Neighbor-Joining (NJ) method. The size of the circle on the branch represents the bootstrap support rate.</p> Full article ">Figure 3 Cont.
<p>Evolutionary tree. (<b>A</b>) The phylogenetic tree of Neverland in different species. The tree is constructed using the Neighbor-Joining (NJ) method. The size of the circle on the branch represents the bootstrap support rate. (<b>B</b>) The phylogenetic tree of P450 family genes involved in 20E metabolism. The trees is constructed using the Neighbor-Joining (NJ) method. The size of the circle on the branch represents the bootstrap support rate.</p> Full article ">Figure 4
<p>The results of the relative expression of genes in different tissues. Note: Hep: hepatopancreas; YO: Y-organ; Mu: muscle; Gill: gill; Cu: cuticle; TG: thoracic ganglion; He: hemocytes; OV: ovary; Te: testes. “*” indicates significant difference between the same or related tissues in different gender (<span class="html-italic">p</span> &lt; 0.05); “**” indicates extremely significant difference between the same or related tissues in different gender (<span class="html-italic">p</span> &lt; 0.01); “ns” indicate no significant difference. (<b>A</b>–<b>E</b>) Different lowercase or uppercase letters indicate significant differences among the different tissues in the same gender.</p> Full article ">
5 pages, 4031 KiB  
Proceeding Paper
In Silico Modeling of the Interaction of NBD Steroids with Insect Steroid-Binding Protein SPC-2
by Hleb Pozniak, Anna Stoliarchuk, Yaroslav Faletrov and Vladimir Shkumatov
Chem. Proc. 2022, 12(1), 86; https://doi.org/10.3390/ecsoc-26-13712 - 18 Nov 2022
Viewed by 1479
Abstract
Two fluorescent sterol-like molecules have been synthesized, namely, a derivative of 7-nitrobenzofurazan (NBD) with 17-(methylamino)-androst-5-en-3beta-ol (DAM-NBD) and NBD-piperazine conjugate with cholesteryl chloroformate (NBD-pip-CCF). In silico docking and plasma membrane simulations have shown ability of the compounds to penetrate the lipid bilayer, as well [...] Read more.
Two fluorescent sterol-like molecules have been synthesized, namely, a derivative of 7-nitrobenzofurazan (NBD) with 17-(methylamino)-androst-5-en-3beta-ol (DAM-NBD) and NBD-piperazine conjugate with cholesteryl chloroformate (NBD-pip-CCF). In silico docking and plasma membrane simulations have shown ability of the compounds to penetrate the lipid bilayer, as well as to bind affinely of the yellow fever mosquito SCP-2 protein structure (code PDB: 3BDQ; binding energy about −11.9 kcal/mol). Also docking with AlphaFOLD-predicted insect Neverland protein structure demonstrated DAM-NBD binding with a similar affinity. The theoretical results indicate perspectives of the fluorescent steroid usage to study insect protein-sterol interactions. Full article
(This article belongs to the Proceedings of The 26th International Electronic Conference on Synthetic Organic Chemistry)
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Figure 1

Figure 1
<p>Structural formulas of fluorescent sterol-like molecules <b>1</b> and <b>2</b>.</p> Full article ">Figure 2
<p>Molecular docking poses of NBD-pip-CCF (<b>A</b>,<b>B</b>) and DAM-NBD (<b>C</b>,<b>D</b>) (<b>A</b>,<b>C</b>—in 2D view; <b>B</b>,<b>D</b>—in 3D view) of structure of SCP-2 of the yellow fever mosquito (PDB 3BDQ). The sub-pictures were build using BioVia software using default settings.</p> Full article ">
18 pages, 405 KiB  
Article
Neverland or Tomorrowland? Addressing (In)compatibility among the SDG Pillars in Europe
by Joana Costa, Diana Cancela and João Reis
Int. J. Environ. Res. Public Health 2021, 18(22), 11858; https://doi.org/10.3390/ijerph182211858 - 12 Nov 2021
Cited by 14 | Viewed by 3230
Abstract
The 2015–2030 agenda framed Sustainable Development as a Universal venture. This decision has a great historic significance as it encompasses building a better future for the whole of humanity, enrolling the millions who have been denied the chance to live a decent, dignified [...] Read more.
The 2015–2030 agenda framed Sustainable Development as a Universal venture. This decision has a great historic significance as it encompasses building a better future for the whole of humanity, enrolling the millions who have been denied the chance to live a decent, dignified and fulfilling life and to achieve their potential. For the first time, an entire generation will have the chance to succeed in ending poverty while being the last to have a chance of saving the planet. The world will be a better place in 2030 if humanity succeeds in this journey. However, there is hovering skepticism around the feasibility of this accomplishment. The article aims to empirically test the (in)compatibilities among these objectives even for developed economies such as the European Union countries, demonstrating that unless solid bridges are built promoting innovative networks at a transnational level, welfare and prosperity among those ecosystems will be compromised. The results show that the Sustainable Development Goals (SDG) pillars have heterogeneous determinants, which are to some extent incompatible. Moreover, policy makers need to further reinforce multi-country compensations if the environment is to be preserved. Full article
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