Search Results (671)

Fumonisins, a class of mycotoxins predominantly produced by Fusarium species, represent a major threat to food safety and public health due to their widespread occurrence in staple crops including peanuts, wine, rice, sorghum, and mainly in maize and maize-based food and feed products. Although fumonisins occur in different groups, the fumonisin B series, particularly fumonisin B1 (FB1) and fumonisin B2 (FB2), are the most prevalent and toxic in this group of mycotoxins and are of public health significance due to the many debilitating human and animal diseases and mycotoxicosis they cause and their classification as by the International Agency for Research on Cancer (IARC) as a class 2B carcinogen (probable human carcinogen). This has made them one of the most regulated mycotoxins, with stringent regulatory limits on their levels in food and feeds destined for human and animal consumption, especially maize and maize-based products. Numerous countries have regulations on levels of fumonisins in foods and feeds that are intended to protect human and animal health. However, there are still gaps in knowledge, especially with regards to the molecular mechanisms underlying fumonisin-induced toxicity and their full impact on human health. Detection of fumonisins has been advanced through various methods, with immunological approaches such as Enzyme-Linked Immuno-Sorbent Assay (ELISA) and lateral flow immunoassays being widely used for their simplicity and adaptability. However, these methods face challenges such as cross-reactivity and matrix interference, necessitating the need for continued development of more sensitive and specific detection techniques. Chromatographic methods, including HPLC-FLD, are also employed in fumonisin analysis but require meticulous sample preparation and derivitization due to the low UV absorbance of fumonisins. This review provides a comprehensive overview of the fumonisin family, focusing on their biosynthesis, occurrence, toxicological effects, and levels of contamination found in foods and the factors affecting their presence. It also critically evaluates the current methods for fumonisin detection and quantification, including chromatographic techniques and immunological approaches such as ELISA and lateral flow immunoassays, highlighting the challenges associated with fumonisin detection in complex food matrices and emphasizing the need for more sensitive, rapid, and cost-effective detection methods. Full article

Background/Objectives: A pilot study was conducted to investigate the effect of four weeks of creatine monohydrate (CrM) on vascular endothelial function in older adults. Methods: In a double-blind, randomized crossover trial, twelve sedentary, healthy older adults were allocated to either the CrM or placebo (PL) group for four weeks, at a dose of 4 × 5 g/day for 5 days, followed by 1 × 5 g/day for 23 days. Macrovascular function (flow-mediated dilation [FMD%], normalized FMD%, brachial-ankle pulse wave velocity [baPWV], pulse wave analysis [PWA]), microvascular function (microvascular reperfusion rate [% StO₂/sec]), and biomarkers of vascular function (tetrahydrobiopterin [BH₄], malondialdehyde [MDA], oxidized low-density lipoprotein [oxLDL], glucose, lipids) were assessed pre- and post-supplementation with a four-week washout period. Results: CrM significantly increased FMD% (pre-CrM, 7.68 ± 2.25%; post-CrM, 8.9 ± 1.99%; p < 0.005), and normalized FMD% (pre-CrM, 2.57 × 10⁻⁴ ± 1.03 × 10⁻⁴%/AUC_SR; post-CrM, 3.42 × 10⁻⁴ ± 1.69 × 10⁻⁴%/AUC_SR; p < 0.05), compared to PL. Microvascular reperfusion rates increased following CrM (pre-CrM, 2.29 ± 1.42%/sec; post-CrM, 3.71 ± 1.44%/sec; p < 0.05), with no change following PL. A significant reduction in fasting glucose (pre-CrM, 103.64 ± 6.28; post-CrM, 99 ± 4.9 mg/dL; p < 0.05) and triglycerides (pre-CrM, 99.82 ± 35.35; post-CrM, 83.82 ± 37.65 mg/dL; p < 0.05) was observed following CrM. No significant differences were observed for any other outcome. Conclusions: These pilot data indicate that four weeks of CrM supplementation resulted in favorable effects on several indices of vascular function in older adults. Full article

Aircraft cabins have high occupant densities and may introduce the risk of COVID-19 contamination. In this study, a segment of a Boeing 767 aircraft cabin with a mixing type of air distribution system was investigated for COVID-19 deposition. A section of a Boeing 737-300 cabin, featuring four rows with 28 box-shaped mannequins, was used for simulation. Conditioned air entered through ceiling inlets and exited near the floor, simulating a mixed air distribution system. Cough droplets were modeled using the Discrete Phase Model from two locations: the centre seat in the second row and the window seat in the fourth row. These droplets had a mean diameter of 90 µm, an exhalation velocity of 11.5 m/s and a flow rate of 8.5 L/s. A high-quality polyhedral mesh of about 7.5 million elements was created, with a skewness of 0.65 and an orthogonality of 0.3. The SIMPLE algorithm and a second-order upwind finite volume method were used to model airflow and droplet dynamics. It was found that the ceiling accounted for the maximum concentration followed by the seats. The concentration of deposits was almost 50% more when the source was at window as compared to the centre seat. The Covid particles resided for longer duration when the source was at the centre of the cabin than when it was located near the widow. Full article

Human factors play a critical role in underground mine evacuations, significantly impacting performance during emergencies. Traditional mine evacuation strategies rely on predetermined routes and static signs, but these methods do not account for the dynamic and unpredictable nature of real emergencies or the diverse behaviors of individuals. This research addresses these limitations by using agent-based simulations to explore evacuation behavior and performance in underground mines. The study involved three key steps. First, agent-based simulations were deployed to model both individual and group behaviors during emergencies. Second, evacuation performance was compared across three scenarios: miners following traditional passive signage, those making decisions in chaotic conditions, and miners equipped with smart evacuation devices. Finally, evacuation times were quantified to assess the effectiveness of each approach. The results revealed that miners using smart devices improved evacuation efficiency by 35% compared with those relying on passive signage and by 37% compared with chaotic decision-making scenarios. The median evacuation time was reduced from 10.8 min with passive signage to 7 min when using smart devices, taking varying stamina levels into account. These findings underscore the importance of integrating intelligent systems that account for pre-evacuation and wayfinding behaviors, offering new insights and setting a higher standard for emergency protocols in underground mining. Full article

Objective: The poultry industry is significantly impacted by viral infections, particularly Newcastle Disease Virus (NDV), which leads to substantial economic losses. It is essential to comprehend how the sequence of development affects biological pathways and how early exposure to infections might affect immune responses. Methods: This study employed transcriptome analysis to investigate host–pathogen interactions by analyzing gene expression changes in NDV-infected chicken embryos’ lungs. Result: RNA-Seq reads were aligned with the chicken reference genome (Galgal7), revealing 594 differentially expressed genes: 264 upregulated and 330 downregulated. The most overexpressed genes, with logFC between 8.15 and 8.75, included C8A, FGG, PIT54, FETUB, APOC3, and FGA. Notably, downregulated genes included BPIFB3 (−4.46 logFC) and TRIM39.1 (−4.26 logFC). The analysis also identified 29 novel transcripts and 20 lncRNAs that were upregulated. Gene Ontology and KEGG pathways’ analyses revealed significant alterations in gene expression related to immune function, metabolism, cell cycle, nucleic acid processes, and mitochondrial activity due to NDV infection. Key metabolic genes, such as ALDOB (3.27 logFC), PRPS2 (2.66 logFC), and XDH (2.15 logFC), exhibited altered expression patterns, while DCK2 (−1.99 logFC) and TK1 (−2.11 logFC) were also affected. Several immune-related genes showed significant upregulation in infected lung samples, including ALB (6.15 logFC), TLR4 (1.86 logFC), TLR2 (2.79 logFC), and interleukin receptors, such as IL1R2 (3.15 logFC) and IL22RA2 (1.37 logFC). Conversely, genes such as CXCR4 (−1.49 logFC), CXCL14 (−2.57 logFC), GATA3 (−1.51 logFC), and IL17REL (−2.93 logFC) were downregulated. The higher expression of HSP genes underscores their vital role in immune responses. Conclusion: Comprehension of these genes’ interactions is essential for regulating viral replication and immune responses during infections, potentially aiding in the identification of candidate genes for poultry breed improvement amidst NDV challenges. Full article

The claustrum is a small but densely interconnected brain structure that is innervated by axons containing serotonin (5-HT), a neuromodulator that has been implicated in control of sleep and in the actions of psychedelic drugs. However, little is known about how 5-HT influences the claustrum. We have combined whole-cell patch-clamp measurements of ionic currents, flash photolysis, and receptor pharmacology to characterize the 5-HT responses of individual claustral projection neurons (PNs) in mouse brain slices. Serotonin application elicited a long-lasting outward current in claustral PNs. This current was due to an increase in membrane permeability to K⁺ ions and was mediated mainly by the type 1A 5-HT receptor (5-HTR-1A). The 5-HT-induced K⁺ current hyperpolarized, and thereby inhibited, the PNs by reducing action potential firing. Focal uncaging of 5-HT revealed that inhibitory 5-HTR-1As were located at both the soma and dendrites of PNs. We conclude that 5-HT creates a net inhibition in the claustrum, an action that should decrease claustrum sensitivity to excitatory input from other brain areas and thereby contribute to 5-HT action in the brain. Full article

How do infants praise the Lord? While we cannot say definitively how this is so, exploring this idea—particularly in the context of intergenerational worship and formation—offers rich theological insights. Scripture declares, “Out of the mouth of babes and nursing infants, you have perfected praise” (Matthew 21:16; Psalm 8:2, NKJV), suggesting that (1) infants indeed praise God, and (2) their praise is perfected by Him. Rather than dismissing this as purely metaphorical, this article draws on St. Augustine’s concept of the jubilus, a song with no intelligible words, to explore how infants’ babbling might be seen as a form of praise and worship, and what we might learn from it if it is thus seen. The article concludes by demonstrating how this reflection on infants’ praise might challenge us to reevaluate and enrich our approaches to Contemporary Praise & Worship and intergenerational formation. Full article

Background and Purpose: activities-based locomotor training (AB-LT) is a restorative therapeutic approach to the treatment of movement deficits in people with non-progressive neurological conditions, including cerebral palsy (CP). Transcutaneous spinal stimulation (TSS) is an emerging tool in the rehabilitation of individuals with sensorimotor deficits caused by neurological dysfunction. This non-invasive technique delivers electrical stimulation over the spinal cord, leading to the modulation of spinal sensorimotor networks. TSS has been used in combination with AB-LT and has been shown to improve muscle activation patterns and enhance motor recovery. However, there are no published studies comparing AB-LT + TSS to AB-LT alone in children with CP. The purpose of this case study was to compare the impact of AB-LT alone versus AB-LT combined with TSS on functional movement and quality of life in a child with CP. Methods: A 13-year-old male with quadriplegic CP participated in this pilot study. He was classified in the Gross Motor Function Classification System (GMFCS) at Level III. He completed 20 sessions of AB-LT (5x/week), then a 2-week washout period, followed by 20 sessions of body-AB-LT + TSS. Treatment sessions consisted of 1 h of locomotor training with body weight support and manual facilitation and 30 min of overground play-based activities. TSS was applied using the RTI Xcite^®, with stimulation at the T11 and L1 vertebral levels. Assessments including the Gross Motor Function Measure (GMFM), 10-m walk test (10 MWT), and Pediatric Balance Scale (PBS) were performed, while spatiotemporal gait parameters were assessed using the Zeno Walkway^®. All assessments were performed at three time points: before and after AB-LT, as well as after AB-LT + TSS. OUTCOMES: After 19/20 sessions of AB-LT alone, the participant showed modest improvements in the GMFM scores (from 86.32 to 88), 10 MWT speed (from 1.05 m/s to 1.1 m/s), and PBS scores (from 40 to 42). Following the AB-LT combined with TSS, scores improved to an even greater extent compared with AB-LT alone, with the GMFM increasing to 93.7, 10 MWT speed to 1.43 m/s, and PBS to 44. The most significant gains were observed in the GMFM and 10 MWT. Additionally, improvements were noted across all spatiotemporal gait parameters, particularly at faster walking speeds. Perhaps most notably, the child transitioned from the GMFCS level III to level II by the end of the study. Discussion: Higher frequency and intensity interventions aimed at promoting neuroplasticity to improve movement quality in children with CP are emerging as a promising alternative to traditional physical therapy approaches. This case study highlights the potential of TSS to augment neuroplasticity-driven treatment approaches, leading to improvements in neuromotor function in children with CP. These findings suggest that TSS could be a valuable addition to rehabilitation strategies, warranting further research to explore its efficacy in larger populations. Full article

Food waste is a pressing global issue, particularly in urban settings, where substantial amounts of surplus food go unused. In corporate environments, this challenge is compounded by the lack of dedicated platforms to facilitate food sharing and reduce waste effectively. This paper examines the current landscape of food waste, existing solutions, and the need for a specialised platform aimed at corporate employees. The proposed solution is the creation of a user-friendly application that enables the sharing of untouched homemade meals. Suppliers can post their meal boxes with details such as location, type of food, and availability status, while consumers can search for and select meal boxes based on their preferences. This paper addresses the gap in solutions for reducing food waste within corporate environments. The meal-box-sharing app provides a practical and sustainable method for minimising food waste and promoting productivity, health, and safety in the workplace. Full article

3D-printed microporous titanium scaffolds enjoy good biointegration with the residuum’s soft and bone tissues, and they promote excellent biomechanical properties in attached prostheses. Implant-associated infection, however, remains a major clinical challenge. Silver-based implant coatings can potentially reduce bacterial growth and inhibit biofilm formation, thereby reducing the risk of periprosthetic infections. In the current study, a 1-µm thick silver coating was prepared on the surface of a 3D-printed microporous titanium alloy with physical vapor deposition (PVD), with a final silver content of 1.00 ± 02 mg/cm². Cell viability was evaluated with an MTT assay of MC3T3-E1 osteoblasts and human dermal fibroblasts cultured on the surface of the implants, and showed low cytotoxicity for cells during the 14-day follow-up period. Quantitative real-time polymerase chain reaction (RT-PCR) analysis of the relative gene expression of the extracellular matrix components (fibronectin, vitronectin, type I collagen) and cell adhesion markers (α2, α5, αV, β1 integrins) in dermal fibroblasts showed that cell adhesion was not reduced by the silver coating of the microporous implants. An RT-PCR analysis of gene expression related to osteogenic differentiation, including TGF-β1, SMAD4, osteocalcin, osteopontin, and osteonectin in MC3T3-E1 osteoblasts, demonstrated that silver coating did not reduce the osteogenic activity of cells and, to the contrary, enhanced the activity of the TGF-β signaling pathway. For representative sample S5 on day 14, the gene expression levels were 7.15 ± 0.29 (osteonectin), 6.08 ± 0.12 (osteocalcin), and 11.19 ± 0.77 (osteopontin). In conclusion, the data indicate that the silver coating of the microporous titanium implants did not reduce the biointegrative or osteoinductive properties of the titanium scaffold, a finding that argues in favor of applying this coating in designing personalized osseointegrated implants. Full article

Yellowtail snapper Ocyurus chrysurus has great potential as a production fish in aquaculture, yet there is very limited information on its nutritional requirements. To establish baseline data, two trials were conducted to evaluate the effects of dietary protein and lipid levels in practical diets on growth and protein retention. The first trial, conducted over 14 weeks, used a series of diets with varying levels of protein (36%, 40%, and 44%) and lipids (6%, 10%, and 14%). The second trial, conducted for 10 weeks, used a series of diets with 36% protein and scaled lipid levels (7%, 10%, 13%, and 16%). Additionally, a commercial reference diet with 44% protein and 12% lipids was included. Growth performance and feed utilization parameters for Trial 1 indicated that the yellowtail snapper were able to effectively utilize practical diets containing 36% protein and 10% lipids, which produced the highest apparent net protein retention (ANPR; %) and survival. No significant differences were found in growth performance metrics, though there were numerical differences in final weight, weight gain, and survival. Similarly, in Trial 2, most growth metrics did not show significant differences. There were variations in weight gain, feed offered, and ANPR, with the highest performance observed in the fish given feed with 13% lipids. Based on the growth performance and ANPR values across these trials, we recommend 36% protein and dietary lipid levels of 7–13%, which are lower than the currently used commercial diets for marine finfish. The data gathered from the current study may be helpful for nutritionists in formulating feed to include more sustainable and cheaper feedstuffs and promote sustainable yellowtail snapper aquaculture production. Full article

Demand side management is a critical issue in the energy sector. Recent events such as the global energy crisis, costs, the necessity to reduce greenhouse emissions, and extreme weather conditions have increased the need for energy efficiency. Thus, accurately predicting energy consumption is one of the key steps in addressing inefficiency in energy consumption and its optimization. In this regard, accurate predictions on a daily, hourly, and minute-by-minute basis would not only minimize wastage but would also help to save costs. In this article, we propose intelligent models using ensembles of convolutional neural network (CNN), long-short-term memory (LSTM), bi-directional LSTM and gated recurrent units (GRUs) neural network models for daily, hourly, and minute-by-minute predictions of energy consumptions in smart buildings. The proposed models outperform state-of-the-art deep neural network models for predicting minute-by-minute energy consumption, with a mean square error of 0.109. The evaluated hybrid models also capture more latent trends in the data than traditional single models. The results highlight the potential of using hybrid deep learning models for improved energy efficiency management in smart buildings. Full article

Prior to the discovery of an APN permutation in six dimension it was conjectured that such functions do not exist in even dimension, as none had been found at that time. However, finding APN permutations in even dimension $\ge 8$ remains a significant challenge. Understanding and determining more properties of these functions is a crucial approach to discovering them. In this note, we study the properties of vectorial Boolean functions based on the weights of the first-order and second-order derivatives of their components. We show that a function is an APN permutation if and only if the sum of the squares of the weights of the first-order derivatives of its components is exactly $2^{2n-1}(2^{n-1}+1)(2^n-1)$. Additionally, we determined that the sum of the weights of the second-order derivatives of the components of any vectorial Boolean function is at most $2^{2n-1}(2^n-1)(2^n-2)$. This bound is achieved if and only if a function is APN. Full article

There is limited information regarding the grain sorghum production trends from early in the millennium towards the 2020s. The main objective of this study was to quantify the grain sorghum production area, economic value, productivity, annual production variation, relationship with changing weather patterns, and yield gap and to identify future areas of intervention and research. The results indicated that the grain sorghum production area in Kansas has increased in the most recent decade (2010–2022) at an average rate of 8 thousand ha year⁻¹. With the current 1.2 million ha harvest area, Kansas continues to allocate more land area for sorghum than any other state in the USA. The average current annual economic value of sorghum in Kansas is USD 0.5 billion. The average sorghum grain productivity for recent years (2000–2022) was 4.3 Mg ha⁻¹ in Kansas. The year-to-year yield variation in the grain sorghum average for Kansas in the years 1929–1956 was ±0.5 Mg ha⁻¹ but increased to ±2 Mg ha⁻¹ for the years 1957–2022. The results also showed a 66 to 96% yield gap between the actual yield (USDA data) and potential non-irrigated yield (Kansas State Grain Sorghum Hybrid Performance Trial data). There was a significant positive correlation between the July–August precipitation and a significant negative correlation between air temperatures and sorghum yield. We conclude that there was an increasing sorghum harvest area trend in Kansas for the years 2010 to 2022. Further research that identifies more unique and important agronomic and economic advantages of sorghum, increasing productivity per unit area across different environments, communicating existing benefits, and developing crop production management best practices are essential to sustain gains in the production area. Full article