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23 pages, 3900 KiB  
Article
A Hybrid Improved Dual-Channel and Dual-Attention Mechanism Model for Water Quality Prediction in Nearshore Aquaculture
by Wenjing Liu, Ji Wang, Zhenhua Li and Qingjie Lu
Electronics 2025, 14(2), 331; https://doi.org/10.3390/electronics14020331 - 15 Jan 2025
Abstract
The aquatic environment in aquaculture serves as the foundation for the survival and growth of aquatic animals, while a high-quality water environment is a necessary condition for promoting efficient and healthy aquaculture development. To effectively guide early warnings and the regulation of water [...] Read more.
The aquatic environment in aquaculture serves as the foundation for the survival and growth of aquatic animals, while a high-quality water environment is a necessary condition for promoting efficient and healthy aquaculture development. To effectively guide early warnings and the regulation of water quality in aquaculture, this study proposes a predictive model based on a dual-channel and dual-attention mechanism, namely, the DAM-ResNet-LSTM model. This model encompasses two parallel feature extraction channels: a residual network (ResNet) and long short-term memory (LSTM), with dual-attention mechanisms integrated into each channel to enhance the model’s feature representation capabilities. Then, the proposed model is trained, validated, and tested using water quality and meteorological parameter data collected by an offshore farm environmental monitoring system. The results demonstrate that the proposed dual-channel structure and dual-attention mechanism can significantly improve the predictive performance of the model. The prediction accuracy for pH, dissolved oxygen (DO), and salinity (SAL) (with Nash coefficients of 0.9361, 0.9396, and 0.9342, respectively) is higher than that for chemical oxygen demand (COD), ammonia nitrogen (NH3-N), nitrite (NO2), and active phosphate (AP) (with Nash coefficients of 0.8578, 0.8542, 0.8372, and 0.8294, respectively). Compared to the single-channel model DA-ResNet (ResNet integrated with the proposed dual-attention mechanism), the Nash coefficients for predicting pH, DO, SAL, COD, NH3-N, NO2, and AP increase by 12.76%, 12.58%, 11.68%, 18.350%, 19.32%, 16%, and 14.99%, respectively. Compared to the single-channel DA-LSTM model (LSTM integrated with the proposed dual-attention mechanism), the corresponding increases in Nash coefficients are 9.15%, 9.93%, 9.11%, 10.91%, 10.11%, 10.39%, and 10.2%, respectively. Compared to the ResNet-LSTM (ResNet and LSTM in parallel) model without the attention mechanism, the improvements in Nash coefficients are 1.91%, 2.4%, 0.74%, 3.41%, 2.71%, 3.55%, and 4.13%, respectively. The predictive performance of the model fulfills the practical requirements for accurate forecasting of water quality in nearshore aquaculture. Full article
15 pages, 1904 KiB  
Article
Preventive Effects of Botulinum Neurotoxin Long-Term Therapy: Comparison of the ‘Experienced’ Benefits and ‘Suspected’ Worsening Across Disease Entities
by Harald Hefter and Sara Samadzadeh
J. Clin. Med. 2025, 14(2), 480; https://doi.org/10.3390/jcm14020480 - 14 Jan 2025
Viewed by 321
Abstract
Background: Repetitive intramuscular injections of botulinum neurotoxin (BoNT) have become the treatment of choice for a variety of disease entities. But with the onset of BoNT therapy, the natural course of a disease is obscured. Nevertheless, the present study tries to analyze patients’ [...] Read more.
Background: Repetitive intramuscular injections of botulinum neurotoxin (BoNT) have become the treatment of choice for a variety of disease entities. But with the onset of BoNT therapy, the natural course of a disease is obscured. Nevertheless, the present study tries to analyze patients’ “suspected” course of disease severity under the assumption that no BoNT therapy had been performed and compares that with the “experienced” improvement during BoNT treatment. Methods: For this cross-sectional study, all 112 BoNT long-term treated patients in a botulinum toxin out-patient department were recruited who did not interrupt their BoNT/A therapy for more than two injection cycles during the last ten years. Patients had to assess the remaining severity of their disease as a percentage of the severity at onset of BoNT therapy and to draw three different graphs: (i) the CoDB-graph showing the course of severity of patient’s disease from onset of symptoms to onset of BoNT/A therapy, (ii) the CoDA-graph illustrating the course of severity from onset of BoNT/A therapy until recruitment, and (iii) the CoDS-graph visualizing the suspected development of disease severity from onset of BoNT/A therapy until recruitment under the assumption that no BoNT/A therapy had been performed. Three different types of graphs were distinguished: the R-type indicated a rapid manifestation or improvement, the C-type a continuous worsening or improvement, and the D-type a delayed manifestation or response to BoNT therapy. Four patient subgroups (cervical dystonia, other cranial dystonia, hemifacial spasm, and the migraine subgroup) comprised 91 patients who produced a complete set of graphs which were further analyzed. The “experienced” improvement and “suspected” worsening of disease severity since the onset of BoNT/A therapy were compared and correlated with demographical and treatment related data. Results: Improvement was significant (p < 0.05) and varied between 45 and 70% in all four patient subgroups, the “suspected” worsening was also significantly (p < 0.05) larger than 0, except in the migraine patients and varied between 10 and 70%. The “total benefit” (sum of improvement and prevented “suspected” worsening) was the highest in the other cranial dystonia group and the lowest in the migraine subgroup. The distributions of R-,C-,D-type graphs across CoDB-, CoDS-, and CoDB-graphs and across the four patient subgroups were significantly different. Conclusions: (i) Most BoNT long-term treated patients have the opinion that their disease would have further progressed and worsened if no BoNT/A therapy had been performed, (ii) The type of response to BoNT/A is different across different subgroups of BoNT/A long-term treated patients. Full article
(This article belongs to the Section Clinical Neurology)
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<p>(<b>A</b>): Three different types (R-, C-, D-type) of graphs are presented indicating the course of disease severity before BoNT-therapy (CoDB-graphs) during the time span (DURS) between onset of symptoms (AOS) to onset of BoNT therapy (AOT). Severity of the disease at onset of BoNT therapy was used as reference (=100%). (<b>B</b>): Three different types (R-, C-, D-type) of graphs indicate the “suspected” course of disease severity under the assumption that no BoNT therapy had been performed (CoDS-graphs) during the time span (DURT) between onset of BoNT therapy (AOT) to the day of recruitment (AGE). (<b>C</b>): Three different types (R-, C-, D-type) of graphs indicate the “experienced” course of disease severity (CoDA-graphs) during the time span (DURT) between onset of BoNT therapy (AOT) to the day of recruitment (AGE).</p>
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<p>Comparative analysis of treatment impact across disease entities. This bar chart represents the mean values of benefit (EBEN-D; left light gray bar), “suspected” worsening (SWORS-D; dark gray bar in the middle), and total benefit (TBEN-D = (EBEN-D) + (SWORS-D; right black bar) for patients with cervical dystonia (CD), other cranial dystonia (CRD), hemifacial spasm (HFS), and migraine (MIG) at the day of recruitment. Standard deviations, indicating the variability of the responses within each disease entity, are presented as error bars.</p>
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<p>Highly significant (chi<sup>2</sup>-testing: <span class="html-italic">p</span> &lt; 0.001) difference in the proportional distributions of three response types (R-, C-, D-type) across the three graph categories (CoDB-, CoDS-, CoDA-graphs). The three pie-charts highlight the relative percentages of R-, C-, and D-response types within the overall CoDB, CoDS, and CoDA graph categories.</p>
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<p>Highly significant (chi<sup>2</sup>-testing: <span class="html-italic">p</span> &lt; 0.001) difference in the proportional distributions of the three response types (R-, C-, D-type) across the four disease entities. These four pie-charts illustrate the proportional distribution of R-,C-, and D-types among the four patient groups (cervical dystonia (CD), other cranial dystonia (CRD), hemifacial spasm (HFS), and migraine (MIG).</p>
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<p>Proportional distributions of the three R-, C-, D-response types across the four disease entities (CD = column 1; CRD = column 2; HFS = column 3; MIG = column 4) for all CoDB-graphs (row 1), all CoDS-graphs (row 2) and all CoDA-graphs (row 3).</p>
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14 pages, 3602 KiB  
Article
Environmental Factors Affecting the Phytoplankton Composition in the Lake of Tibetan Plateau
by Qinghuan Zhang, Zijian Xie, Chunhua Li, Chun Ye, Yang Wang, Zishu Ye, Weiwei Wei and Hao Wang
Diversity 2025, 17(1), 47; https://doi.org/10.3390/d17010047 - 13 Jan 2025
Viewed by 350
Abstract
Due to the high altitude, unique geographical location, difficult accessibility and low temperature, the environmental factors influencing phytoplankton composition have rarely been investigated in the Selin Co Lake, which is the largest lake in the Tibetan Plateau. Phytoplankton composition can indicate aquatic ecosystem [...] Read more.
Due to the high altitude, unique geographical location, difficult accessibility and low temperature, the environmental factors influencing phytoplankton composition have rarely been investigated in the Selin Co Lake, which is the largest lake in the Tibetan Plateau. Phytoplankton composition can indicate aquatic ecosystem conditions, which may be sensitive to environmental factors in the Tibetan Plateau. In this study, we investigated the main environmental factors that influence phytoplankton species in the Selin Co Lake by analyzing the spatial distribution and applying statistical analyses. We also compared the influential environmental factors in this lake with other lakes around the world. The results suggest that the eleven environmental variables can explain about 46.78% of the phytoplankton’s composition. DO and fluoride were the most significant environmental variables, followed by arsenic and COD, and the other variables had comparatively smaller and more insignificant influences on phytoplankton composition. There were five dominant phytoplankton species in the Selin Co Lake, namely, Microcystis sp., Navicula spp., Chlorella vulgaris, Ankistrodesmus falcatus, and Westella sp. Some of these dominant species were also found in other tropical lakes, suggesting that the phytoplankton community could adapt to environmental changes. A clear understanding of the influential environmental variables affecting phytoplankton composition could help us to make proper water quality protection strategies in future climate change scenarios. Full article
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<p>Geographical location of the Selin Co Lake and the sampling points of lake water (S1–S21).</p>
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<p>Spatial distribution of physico-chemical parameters in Selin Co Lake. (<b>a</b>) Water depth, (<b>b</b>) water transparency, (<b>c</b>) dissolved oxygen (DO), (<b>d</b>) water temperature, (<b>e</b>) TN concentrations, (<b>f</b>) TP concentrations, (<b>g</b>) COD<sub>Mn</sub>, (<b>h</b>) Trophic level index, (<b>i</b>) TDS, (<b>j</b>) salt content (salinity), (<b>k</b>) arsenic content, and (<b>l</b>) fluoride content.</p>
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<p>Correlation heatmaps of environmental variables in Selin Co Lake (in the circles, * represents <span class="html-italic">p</span> &lt; 0.05, ** represents <span class="html-italic">p</span> &lt; 0.01, and *** represents <span class="html-italic">p</span> &lt; 0.001; correlation coefficients close to 0 are shown as blank; salt is salinity, and SD is water transparency).</p>
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<p>(<b>a</b>) The relative abundance and (<b>b</b>) the relative biomass of different phytoplankton groups at each sampling point.</p>
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<p>RDA analysis of phytoplankton species abundance and environmental factors in Selin Co Lake. Black circles represent sampling points. Blue arrows represent environmental variables, and red arrows represent phytoplankton groups. The lengths of the arrows indicate how much variance was explained by the corresponding variable. The angles between arrows indicate correlations between individual environmental variables. SD represents water transparency.</p>
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26 pages, 6617 KiB  
Article
Optimization of Solar Corrosion Fenton Reactor for the Recovery of Textile Wastewater: In Situ Release of Fe2+
by Ana Fernanda Tenorio-Hernández, Ivonne Linares-Hernández, Luis Antonio Castillo-Suárez, Verónica Martínez-Miranda and Carolina Álvarez-Bastida
Catalysts 2025, 15(1), 63; https://doi.org/10.3390/catal15010063 - 12 Jan 2025
Viewed by 407
Abstract
A Solar Corrosion Fenton reactor (SCFr) was developed by packing an iron-carbon steel filament inside the reactor to enable the in situ release of Fe2+. A Box–Behnken experimental design was used to optimize the effect of HRT (20, 30, and 40 [...] Read more.
A Solar Corrosion Fenton reactor (SCFr) was developed by packing an iron-carbon steel filament inside the reactor to enable the in situ release of Fe2+. A Box–Behnken experimental design was used to optimize the effect of HRT (20, 30, and 40 min), the mass ratios of the packed filament inside the reactor with respect to volume (0.1, 0.2, 0.3 w/v), and the peroxide dosage added (500, 1000, and 1500 mg/L), the response variables were the percentage removal of COD, color, and turbidity. The optimum conditions for SCFr were an HRT of 24.5 min, a ratio of 0.16 (0.0032 m2/L), and a peroxide dose of 1006.9 mg/L. The removal was 91.8%, 98.4%, and 87.3% COD, color, and turbidity, respectively. Without solar radiation, the percentage removal was reduced by 16.3%, 47.9%, and 34.0% in terms of COD, color, and turbidity, respectively. The concentration of Fe2+ released was 25.4 mg/L of Fe2+. Prolonged HRT increases Fe2+ concentration and turbidity, which increase COD. The oxidation kinetics were fitted to a Behnajady–Modirshahla–Ghanbery (BMG) model, which indicated a high oxidation rate that is reflective of low treatment times. The w/v ratio was the most significant factor; the release of Fe2+ was stimulated by UV radiation and the chloride concentration of wastewater, which prevents the formation of an oxide layer, thus allowing its continuous release, taking advantage of solar radiation and the pH and chloride concentration of the raw sample. Full article
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Figure 1
<p>Response surfaces for the percentage of COD removal at optimum operating conditions. (<b>a</b>) interaction effect ratio vs. H<sub>2</sub>O<sub>2</sub>, (<b>b</b>) interaction effect HRT vs. ratio, (<b>c</b>) interaction effect HRT vs. H<sub>2</sub>O<sub>2</sub>. <span style="color:#4472C4">□</span> experimental data, <span style="color:red">□</span> maximum estimated efficiency.</p>
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<p>Response surfaces for the percentage of color removal at optimum operating conditions. (<b>a</b>) interaction effect ratio vs. H<sub>2</sub>O<sub>2</sub>, (<b>b</b>) interaction effect HRT vs. ratio, (<b>c</b>) interaction effect HRT vs. H<sub>2</sub>O<sub>2</sub>. <span style="color:#4472C4">□</span> experimental data, <span style="color:red">□</span> maximum estimated efficiency.</p>
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<p>Response surfaces for the percentage of turbidity removal at optimum operating conditions. (<b>a</b>) interaction effect ratio vs. H<sub>2</sub>O<sub>2</sub>, (<b>b</b>) interaction effect HRT vs. ratio, (<b>c</b>) interaction effect HRT vs. H<sub>2</sub>O<sub>2</sub>. <span style="color:#4472C4">□</span> experimental data, <span style="color:red">□</span> maximum estimated efficiency.</p>
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<p>BMG fitted kinetic model (green line) as a function of (<b>a</b>) COD, (<b>b</b>) color, and (<b>c</b>) turbidity vs. accumulated energy: (•) experimental data, (◦) calculated data, <span style="color:#7030A0">---</span> the kinetic phases.</p>
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<p>Efficiency of the SCFr system in the presence and absence of sunlight, operating conditions: HRT of 24.5 min, a ratio of 0.16, and a peroxide dosage of 1006.9 mg/L. <span style="color:#4472C4">▄</span> No sunlight (H<sub>2</sub>O<sub>2</sub>/Fe<sup>2+</sup>), <span style="color:#B4C6E7">▄</span> SCFr (H<sub>2</sub>O<sub>2</sub>/Fe<sup>2+</sup>/UV solar).</p>
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<p>(<b>a</b>) Effect of light, pH, temperature, and solar radiation on Fe<sup>2+</sup> release and oxide formation at HRT 24.6 min, a 0.16 ratio, and without a dose of H<sub>2</sub>O<sub>2</sub>. (<b>b</b>) Fe and O content, values with the same letter do not significantly differ (A, B, C and D by Oxigen, a, b, c, d, and e by Iron); Tukey test (<span class="html-italic">p</span> &lt; 0.05), and (<b>c</b>) possible pitting mechanism on the filament surface.</p>
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<p>(<b>a</b>) Effect of light, pH, temperature, and solar radiation on Fe<sup>2+</sup> release and oxide formation at HRT 24.6 min, a 0.16 ratio, and without a dose of H<sub>2</sub>O<sub>2</sub>. (<b>b</b>) Fe and O content, values with the same letter do not significantly differ (A, B, C and D by Oxigen, a, b, c, d, and e by Iron); Tukey test (<span class="html-italic">p</span> &lt; 0.05), and (<b>c</b>) possible pitting mechanism on the filament surface.</p>
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<p>SEM micrographs of carbon steel strands packed in the SCFr at a scale of 10 µm, ×1000: (<b>a</b>) carbon steel filament (without treatment), (<b>b</b>) acid corrosion, (<b>c</b>) UVA-LED, (<b>d</b>) temperature, (<b>e</b>) temperature-UVA-LED, (<b>f</b>) temperature-Cl<sup>−</sup>, (<b>g</b>) temperature-UVA-LED+Cl<sup>−</sup>, and (<b>h</b>) SCFr (temperature-UVA solar+Cl<sup>−</sup>).<math display="inline"><semantics> <mrow> <mo mathcolor="red">→</mo> </mrow> </semantics></math> points out areas of corrosion and pitting.</p>
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<p>SEM micrographs of carbon steel strands packed in the SCFr at a scale of 10 µm, ×1000: (<b>a</b>) carbon steel filament (without treatment), (<b>b</b>) acid corrosion, (<b>c</b>) UVA-LED, (<b>d</b>) temperature, (<b>e</b>) temperature-UVA-LED, (<b>f</b>) temperature-Cl<sup>−</sup>, (<b>g</b>) temperature-UVA-LED+Cl<sup>−</sup>, and (<b>h</b>) SCFr (temperature-UVA solar+Cl<sup>−</sup>).<math display="inline"><semantics> <mrow> <mo mathcolor="red">→</mo> </mrow> </semantics></math> points out areas of corrosion and pitting.</p>
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<p>System of SCFr: (a) influent, (b) peroxide feed, (c) SCFr, (d) Mirosun<sup>®</sup> films, (e) carbon steel filament, (f) Kimax<sup>®</sup> borosilicate tube, (g) thermocouple, and (h) effluent.</p>
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<p>The experimental values and level of independent variables (<b>a</b>) and (<b>b</b>) special distribution of the points of the experimental matrix for 3 factors; <span style="color:red">*</span> central point, <span style="color:blue">■</span> factorial point.</p>
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20 pages, 118630 KiB  
Article
Wastewater Treatment with Geotextile Filters: The Role in Permeability and Pollutant Control
by Maria Vitoria Morais, Leonardo Marchiori, Josivaldo Sátiro, Antonio Albuquerque and Victor Cavaleiro
Appl. Sci. 2025, 15(2), 626; https://doi.org/10.3390/app15020626 - 10 Jan 2025
Viewed by 342
Abstract
The application of geotextiles as filter materials in various systems, such as biofilters, wetlands, and wastewater treatment plants, has grown significantly in recent years. The ability of these materials to support biofilm growth makes them ideal for the removal of organic and inorganic [...] Read more.
The application of geotextiles as filter materials in various systems, such as biofilters, wetlands, and wastewater treatment plants, has grown significantly in recent years. The ability of these materials to support biofilm growth makes them ideal for the removal of organic and inorganic contaminants present in wastewater. The objective of this research was to analyze clogging through variations in permeability, using column tests for 80 days with two types of nonwoven geotextiles with different grammages (GT120 and GT300), as well as to study the efficiency in the removal of organic matter. A synthetic wastewater was used, allowing the specific observation of biological clogging and the treatment carried out exclusively by microorganisms. The results indicated that bioclogging was not a significant factor within the experimental period. Through the mass test, a continuous increase in biofilm growth over time was observed for both geotextiles. For scanning electron microscopic (SEM) images, GT300 presented a larger biofilm area. A higher removal of COD (80%), N (52%), and P (36%) by microorganisms present in GT300 was found, which appears to be associated with its greater thickness and weight. The higher mesh density provides a larger area for the growth of microorganisms, allowing a greater amount of biomass to establish itself and contributing to the efficient removal of pollutants. These findings highlight the potential of using geotextile filters in wastewater treatment applications, where biofilm growth can positively contribute to contaminant removal without immediately compromising permeability. Full article
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<p>Setup permeameters used in the tests.</p>
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<p>Permeability variation for GT120.</p>
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<p>Permeability variation for GT300.</p>
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<p>Variation in COD removal in permeameters with GT120 and GT300.</p>
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<p>Variation in NH4-N removal in permeameters with GT120 and GT300.</p>
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<p>Variation in PO4-P removal in permeameters with GT120 and GT300.</p>
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<p>(<b>a</b>,<b>b</b>) pH, (<b>c</b>,<b>d</b>) conductivity, (<b>e</b>,<b>f</b>) dissolved oxygen, and (<b>g</b>,<b>h</b>) temperature parameters measured on the GT120 and GT300.</p>
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<p>Results found for measuring biomass growth.</p>
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<p>Layout used for SEM images.</p>
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<p>SEM images for the GT120—S1: (<b>a</b>) top view of the center, (<b>b</b>) top view of the middle, (<b>c</b>) top view of the edge, (<b>d</b>) cross-sectional view of the center, (<b>e</b>) cross-sectional view of the middle, (<b>f</b>) cross-sectional view of the edge.</p>
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<p>SEM images for the GT120—S2: (<b>a</b>) top view of the center, (<b>b</b>) top view of the middle, (<b>c</b>) top view of the edge, (<b>d</b>) cross-sectional view of the center, (<b>e</b>) cross-sectional view of the middle, (<b>f</b>) cross-sectional view of the edge.</p>
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<p>SEM images for the GT120—S3: (<b>a</b>) top view of the center, (<b>b</b>) top view of the middle, (<b>c</b>) top view of the edge, (<b>d</b>) cross-sectional view of the center, (<b>e</b>) cross-sectional view of the middle, (<b>f</b>) cross-sectional view of the edge.</p>
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<p>SEM images for the GT120—S4: (<b>a</b>) top view of the center, (<b>b</b>) top view of the middle, (<b>c</b>) top view of the edge, (<b>d</b>) cross-sectional view of the center, (<b>e</b>) cross-sectional view of the middle, (<b>f</b>) cross-sectional view of the edge.</p>
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<p>SEM images for the GT300—S1: (<b>a</b>) top view of the center, (<b>b</b>) top view of the middle, (<b>c</b>) top view of the edge, (<b>d</b>) cross-sectional view of the center, (<b>e</b>) cross-sectional view of the middle, (<b>f</b>) cross-sectional view of the edge.</p>
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<p>SEM images for the GT300—S2: (<b>a</b>) top view of the center, (<b>b</b>) top view of the middle, (<b>c</b>) top view of the edge, (<b>d</b>) cross-sectional view of the center, (<b>e</b>) cross-sectional view of the middle, (<b>f</b>) cross-sectional view of the edge.</p>
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<p>SEM images for the GT300—S2: (<b>a</b>) top view of the center, (<b>b</b>) top view of the middle, (<b>c</b>) top view of the edge, (<b>d</b>) cross-sectional view of the center, (<b>e</b>) cross-sectional view of the middle, (<b>f</b>) cross-sectional view of the edge.</p>
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<p>SEM images for the GT300—S3: (<b>a</b>) top view of the center, (<b>b</b>) top view of the middle, (<b>c</b>) top view of the edge, (<b>d</b>) cross-sectional view of the center, (<b>e</b>) cross-sectional view of the middle, (<b>f</b>) cross-sectional view of the edge.</p>
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<p>SEM images for the GT300—S4: (<b>a</b>) top view of the center, (<b>b</b>) top view of the middle, (<b>c</b>) top view of the edge, (<b>d</b>) cross-sectional view of the center, (<b>e</b>) cross-sectional view of the middle, (<b>f</b>) cross-sectional view of the edge.</p>
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16 pages, 4247 KiB  
Article
Removal of TP, COD, and NH4+-N in Simulated Slaughtering Wastewater by Two Kinds of Immobilized Microalgal Spheres
by Wei Xu, Xiaoping Zhang, Guichang Zhang and Xin Zhang
Water 2025, 17(2), 179; https://doi.org/10.3390/w17020179 - 10 Jan 2025
Viewed by 322
Abstract
The treatment of wastewater using microalgae is regarded as a green and potential technology. However, its engineering application has been largely hindered because of the limitation of microalgae separation and harvesting. Therefore, immobilization technology has been widely used to embed microalgae for wastewater [...] Read more.
The treatment of wastewater using microalgae is regarded as a green and potential technology. However, its engineering application has been largely hindered because of the limitation of microalgae separation and harvesting. Therefore, immobilization technology has been widely used to embed microalgae for wastewater treatment. In this paper, sodium alginate (SA) and polyvinyl alcohol (PVA) as the common immobilized carriers were used to immobilize ankistrodesmus falcatus for simulated slaughtering wastewater (SSW) treatment. The experimental results of the mass transfer and adsorption of immobilized carriers were found to show that the mass transfer of SA-SiO2 gel balls (SS-GB) was better than PVA-SA gel balls (PS-GB) and that the adsorption of PS-GB was better than SS-GB. When immobilizing microalgae with the two kinds of carriers, it was found that SA-SiO2 microalgal spheres (SS-MS) were better than PVA-SA microalgal spheres (PS-MS) for the maintenance of microalgal cell activity and that PS-MS were better than SS-MS for the resistance to biodegradation. This is because the carrier of PS-MS had a thick shell and dense structure, while the carrier of SS-MS had a thin shell and loose structure. The results of SSW treatment by PS-MS and SS-MS were found to show that the total phosphorus (TP) removal rates of PS-MS and SS-MS were 90.31% and 86.60%, respectively. This indicates that the TP removal effect of PS-MS was superior to that of SS-MS. The adsorption kinetics simulation showed that the adsorption of TP onto PS-GB was controlled by chemisorption and that the adsorption of TP onto SS-GB was controlled by physical adsorption. The chemical oxygen demand (COD) and ammonium nitrogen (NH4+-N) removal of PS-MS were 9.30% and 10.70%, respectively, and the COD and NH4+-N removal of SS-MS were 54.60% and 62.08%, respectively. This indicates that the COD and NH4+-N removal effect of SS-MS were superior to PS-MS. This is the result of the combined action of the degradation by microalgal cells and adsorption by the carrier. Full article
(This article belongs to the Section Wastewater Treatment and Reuse)
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<p>The cells of <span class="html-italic">ankistrodesmus falcatus</span> under the microscope.</p>
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<p>Two kinds of immobilized microalgal spheres (<b>a</b>) SS-MS; (<b>b</b>) PS-MS.</p>
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<p>Drawing of the experimental facility.</p>
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<p>SEM of two kinds of gel balls. (<b>a</b>) PS-GB, 31×; (<b>b</b>) PS-GB, 500×; (<b>c</b>) PS-GB, 2.00 k×; (<b>d</b>) SS-GB, 40×; (<b>e</b>) SS-GB, 500×; (<b>f</b>) SS-GB, 2.00 k×.</p>
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<p>FTIR of two kinds of gel balls.</p>
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<p>The mass transfer and adsorption properties of two kinds of gel balls. (<b>a</b>) Mass transfer and (<b>b</b>) adsorption.</p>
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<p>Microscopic observations of two kinds of gel balls at 10× magnification. (<b>a</b>) SS-GB and (<b>b</b>) PS-GB.</p>
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<p>Comparison of resistance to the biodegradation of two immobilized microalgal spheres. (<b>a</b>) SS-MS and (<b>b</b>) PS-MS.</p>
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<p>Change of microalgal cell density, pH and DO values in two kinds of immobilized microalgal spheres.</p>
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<p>The effect of SSW treatment by immobilized microalgal spheres and gel balls. (<b>a</b>) COD; (<b>b</b>) NH<sub>4</sub><sup>+</sup>-N; (<b>c</b>) TP; (<b>d</b>) pH.</p>
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<p>Fitting of the pseudo-first-order kinetics equation and the pseudo-second-order kinetics equation. (<b>a</b>) SS-MS and (<b>b</b>) PS-MS.</p>
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<p>Treatment mechanism of SSW by two kinds of immobilized microalgal spheres. (<b>a</b>) SS-MS and (<b>b</b>) PS-MS.</p>
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14 pages, 2112 KiB  
Article
Performance of Integrated Biofilm-Phytoremediation Process in Reclaiming Water from Domestic Wastewater
by Fairuz Afiqah Buslima, Hassimi Abu Hasan, Jahira Alias, Jaga Sahsiny Jaganathan, Junaidah Buhari, Suriya Vathi Subramanian and Siti Rozaimah Sheikh Abdullah
Water 2025, 17(2), 163; https://doi.org/10.3390/w17020163 - 9 Jan 2025
Viewed by 486
Abstract
The rapid development of the residential and industrial sectors produces a huge amount of treated domestic wastewater. The treated wastewater is discharged and could affect the environment in the long term. Improving the quality of treated domestic wastewater for water reclamation would benefit [...] Read more.
The rapid development of the residential and industrial sectors produces a huge amount of treated domestic wastewater. The treated wastewater is discharged and could affect the environment in the long term. Improving the quality of treated domestic wastewater for water reclamation would benefit both sectors. This study aims to determine the efficiency of the biofilm-phytoremediation integration process in reclaiming domestic wastewater. A cuboid-shaped reactor was filled with 15 L of domestic wastewater, utilizing water hyacinth and a polyethylene carrier as supporting media for the process. The integrated reactor is tested in two phases: the initial adaptation of bacteria with domestic and synthetic wastewater (Phase I) and the integration process of biofilm-phytoremediation, based on the factors of NH3-N concentration and hydraulic retention time (HRT), for 24 to 48 h (Phase II). In Phase II, pollutant removal was observed at varying NH3-N concentrations: C1 (11–13 mg/L), C2 (9–11 mg/L), and C3 (3–5 mg/L). The study’s findings indicate a consistent performance in the first phase, with removal rates for COD and NH3-N ranging between 86.7–100.0% and 79.0–99.6%, respectively. The reactor effectively removed pollutants at varying concentrations of NH3-N, with average removal up to 100% (COD), 99% (NH3-N), and 80% (PO43−). This integrated reactor shows the finest treated water quality outcomes for non-potable water recovery, as well as offers an alternative to resolve water scarcity for use in various sectors. Full article
(This article belongs to the Section Wastewater Treatment and Reuse)
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<p>(<b>a</b>) Schematic drawing of integrated biofilm-phytoremediation reactor. (<b>b</b>) Top view.</p>
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<p>Acclimatization process of the integrated reactor: (<b>a</b>) chemical oxygen demand (COD); (<b>b</b>) ammonia–nitrogen (NH<sub>3</sub>-N); (<b>c</b>) MLSS and MLVSS.</p>
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<p>NH<sub>3</sub>-N removal at various initial concentrations.</p>
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<p>Average removal of COD.</p>
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<p>Average removal of PO<sub>4</sub><sup>3−</sup>.</p>
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16 pages, 3592 KiB  
Article
Presence of Humic Acids in Landfill Leachate and Treatment by Flocculation at Low pH to Reduce High Pollution of This Liquid
by Carlos Costa, M. Laura Pinedo and Brayan D. Riascos
Sustainability 2025, 17(2), 481; https://doi.org/10.3390/su17020481 - 9 Jan 2025
Viewed by 639
Abstract
Humic substances are abundant in landfill leachate, especially humic acids, which are insoluble at low pH in aqueous solutions. Focusing on the chemical properties of humic acids, we describe in this work a new method for a sustainable treatment of landfill leachate originated [...] Read more.
Humic substances are abundant in landfill leachate, especially humic acids, which are insoluble at low pH in aqueous solutions. Focusing on the chemical properties of humic acids, we describe in this work a new method for a sustainable treatment of landfill leachate originated from solid wastes, which consists of the reduction of organic load (COD, chemical oxygen demand) and colour and is based in the gradual decrease in pH to the value in which HAs are insoluble in water solution. Zeta potential values mark the chemical stage of humic acids because of ionisation–protonation of the phenolic and carboxylic groups, and this parameter is monitored during flocculation, changing from −16.8 mV at pH 7.7 to 0.0068 mV at pH 2.0, when HAs precipitate. The final result is the reduction in the organic matter content (COD) and colour in the leachate, 86.1% and 84.7%, respectively. Solids produced by precipitation during the acidification treatment have been characterized by elemental chemical analysis and Fourier transform infrared spectrometry, concluding a high similarity in chemical composition with commercial and natural humic acids. Protonated humic acids at low pH can interact with other molecules by hydrogen bonds and form bigger molecular structures much more unstable in suspension, which conduct to precipitation. The mean diameter of the humic acids aggregates was measured, detecting the formation of big molecular structures at low pH. This process is analysed and compared economically with other processes proposed for landfill leachate treatment, resulting in a promising technique for the management of this residue. Full article
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<p>Chromaticity diagram for landfill leachate, where x = 0.223 and y = 0.205 are the trichromatic coefficients obtained by spectrophotometric method for colour analysis (2120C, [<a href="#B39-sustainability-17-00481" class="html-bibr">39</a>]). Dominant wavelength, obtained by the union of the achromatic point and the trichromatic coefficients point, is 474 nm (dark blue colour).</p>
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<p>Concentration of metal ions in landfill leachate (metals, transition metals, and metalloids), obtained in one sample from the WTC (average relative SD 2%). Metals and metalloids are shown in the horizontal axe and concentration in leachate (mg/L) in the vertical axe, with the numerical value of concentration over the corresponding bar. Cd<sup>2+</sup> and Hg<sup>2+</sup> were detected in trace concentrations (&lt;3 μg/L and &lt;0.1 μg/L, respectively).</p>
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<p>Results of flocculation of landfill leachate at low pH in COD (<b>a</b>) and colour reduction (<b>b</b>). Error bars represent SD of three independent measurements. Nucleation occurs in colour reduction graph (<b>b</b>) at pH 4.5. Numerical values of the graphs in <a href="#app1-sustainability-17-00481" class="html-app">Supplementary Materials</a>, <a href="#app1-sustainability-17-00481" class="html-app">Table S1</a>.</p>
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<p>Results of the analysis of Z potential during the flocculation of landfill leachate at low pH, from the initial value of pH 7.7 in the leachate, to the final value of pH 2.0, in the graph from the left to the right. Horizontal axis represents pH, and vertical axis on the right refers to Z potential value in mV. Error bars represent SD of three independent measurements. Numerical values of Z potential in <a href="#app1-sustainability-17-00481" class="html-app">Supplementary Materials</a>, <a href="#app1-sustainability-17-00481" class="html-app">Table S1</a>.</p>
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<p>Particle size (mean diameter, nm) during flocculation at low pH of landfill leachate, mean ± SD (triplicate). Formation of big aggregates by intermolecular H-bonds is confirmed at pH 3.0 and 2.0 (yellow bars).</p>
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<p>Proposal of the aggregation of humic acid molecules (HA) from landfill leachate protonated at low pH by intermolecular interaction and the formation of H-bonds (interrupted green lines).</p>
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<p>Elemental chemical analysis (carbon, hydrogen, nitrogen, and sulphur: CHNS) of the precipitate of the landfill leachate after treatment at low pH (humic acids). The vertical axis represents percentage of the element included in the horizontal axis. HA1 and HA2 are two independent samples collected from the leachate precipitate. Aldrich humic acid (AHA) and humic acids from the literature are included for comparison (H Aldrich, HA aquatic, and HA terrestrial, [<a href="#B24-sustainability-17-00481" class="html-bibr">24</a>]). Analysis of HAs (HA1 and HA2) and AHA were performed in triplicate and error bars record SD. For the external data (<span class="html-italic">HA aquatic</span> and <span class="html-italic">terrestrial</span>, from the literature), error bars represent the range in which the value is recorded.</p>
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<p>FT-IR spectra of humic acids proceed from the precipitate of landfill leachate (HAs, red line) and Aldrich humic acid (AHA, black line). Characteristic signals of humic acids are highlighted, red dashed line only for HAs in landfill leachate.</p>
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24 pages, 4223 KiB  
Article
Research on Enhancing Domestic Wastewater Treatment in the Heterotrophic Nitrification–Aerobic Denitrification-Based Anaerobic/Oxic Biofilm System
by Yingbao Wu, Biaoyi Wang, Ziyi Ou, Peiqin Peng, Miaomiao Zhang, Shunan Zhang and Feng Liu
Water 2025, 17(2), 162; https://doi.org/10.3390/w17020162 - 9 Jan 2025
Viewed by 330
Abstract
Traditional wastewater treatment processes still encounter challenges such as the limited treatment efficiency and excessive greenhouse gas emissions, which restrict their application in environmentally sustainable practices. This study developed an A/O biofilm system and assessed the impact of inoculating the system with the [...] Read more.
Traditional wastewater treatment processes still encounter challenges such as the limited treatment efficiency and excessive greenhouse gas emissions, which restrict their application in environmentally sustainable practices. This study developed an A/O biofilm system and assessed the impact of inoculating the system with the heterotrophic nitrification–aerobic denitrification (HN–AD) strain Alcaligenes faecalis WT14 on pollutant removal efficiency and greenhouse gas emissions. A continuous monitoring experiment was conducted over 140 days, comparing the system inoculated with WT14 (the TWT14 system) and the non-inoculated system (the CK system). The results demonstrated that the TWT14 system outperformed the CK system in pollutant removal, with higher NH₄⁺-N, TN, and COD removal efficiencies of 11.22%, 21.96%, and 12.51%, respectively, and the quality of discharge water from TWT14 maintaining compliance with national discharge standards. This improvement underscores the positive impact of inoculation with the WT14 strain on enhancing the pollutant removal performance of the A/O biofilm system. Regarding greenhouse gas emissions, the TWT14 system exhibited a significantly higher N₂O emission flux in the aeration tank compared with the CK system, while CO₂ and CH₄ emissions were predominantly concentrated in the anaerobic tank. Global warming potential (GWP) analysis showed no significant difference in the total average GWP between the two systems. However, the TWT14 system demonstrated a lower GWP per unit of TN removed, highlighting its superior ecological benefits. Environmental factor analysis revealed that the temperature, pH, humidity, and salinity had significant impacts on both pollutant removal efficiency and greenhouse gas emissions. Additionally, microbial community analysis indicated that inoculation with the WT14 strain enhanced microbial diversity and richness within the A/O biofilm system, with Alcaligenes and norank_f_JD30-KF-CM45 playing key roles in nitrogen removal. This study provides valuable insights for optimizing A/O biofilm system design and offers scientific guidance for the sustainable upgrading of wastewater treatment technologies. Full article
(This article belongs to the Special Issue Biological Wastewater Treatment Process and Nutrient Recovery)
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<p>Schematic diagram of the test setup.</p>
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<p>Pollutant concentration and removal rate of effluent from A/O biofilm system, where (<b>a</b>) shows ammonia concentration and removal rate, (<b>b</b>) shows TN concentration and removal rate, and (<b>c</b>) shows COD concentration and removal rate.</p>
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<p>Greenhouse gas emission fluxes from aeration tanks (<b>a</b>) and oxic tanks (<b>b</b>) of A/O biofilm systems.</p>
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<p>Heat map of correlation between environmental factors and water quality change and gas emission in the anaerobic tank (<b>a</b>) and the oxic tank (<b>b</b>).</p>
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<p>Composition of microbiological phyla and genera at different stages of operation. (<b>a</b>) New information on the abundance and diversity of microflora in biofilm samples (lower case letters indicate statistically significant differences, <span class="html-italic">p</span> &lt; 0.05). (<b>b</b>,<b>c</b>) The composition and distribution of bacterial colonies at the phylum taxonomic level (TOP10), respectively. (<b>d</b>) A heat map of the distribution of bacterial colonies at the genus level. Pentagrams label HN–AD bacteria, and triangles label denitrifying bacteria.</p>
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<p>Composition of microbiological phyla and genera at different stages of operation. (<b>a</b>) New information on the abundance and diversity of microflora in biofilm samples (lower case letters indicate statistically significant differences, <span class="html-italic">p</span> &lt; 0.05). (<b>b</b>,<b>c</b>) The composition and distribution of bacterial colonies at the phylum taxonomic level (TOP10), respectively. (<b>d</b>) A heat map of the distribution of bacterial colonies at the genus level. Pentagrams label HN–AD bacteria, and triangles label denitrifying bacteria.</p>
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<p>Composition of microbiological phyla and genera at different stages of operation. (<b>a</b>) New information on the abundance and diversity of microflora in biofilm samples (lower case letters indicate statistically significant differences, <span class="html-italic">p</span> &lt; 0.05). (<b>b</b>,<b>c</b>) The composition and distribution of bacterial colonies at the phylum taxonomic level (TOP10), respectively. (<b>d</b>) A heat map of the distribution of bacterial colonies at the genus level. Pentagrams label HN–AD bacteria, and triangles label denitrifying bacteria.</p>
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<p>Heat map of correlation of bacterial genera with pollutant concentrations, GHG emissions, and environmental factors. ** indicates significant correlation at the 0.01 level (two-sided); * indicates significant correlation at the 0.05 level (two-sided).</p>
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10 pages, 4329 KiB  
Article
Structure of Plant Populations in Constructed Wetlands and Their Ability for Water Purification
by Junshuang Yu, Ling Xian and Fan Liu
Plants 2025, 14(2), 162; https://doi.org/10.3390/plants14020162 - 8 Jan 2025
Viewed by 329
Abstract
In constructed wetlands (CWs) with multiple plant communities, population structure may change over time and these variations may ultimately influence water quality. However, in CWs with multiple plant communities, it is still unclear how population structure may change over time and how these [...] Read more.
In constructed wetlands (CWs) with multiple plant communities, population structure may change over time and these variations may ultimately influence water quality. However, in CWs with multiple plant communities, it is still unclear how population structure may change over time and how these variations ultimately influence water quality. Here, we established a CW featuring multiple plant species within a polder to investigate the variation in plant population structure and wastewater treatment effect for drainage water over the course of one year. Our results showed that the total species decreased from 52 to 36; however, 20 established species with different ecological types (emerged or submerged) remained with the same functional assembly for nutrient absorption, accounting for 94.69% of relative richness at the initial stage and 91.37% at the last state. The Shannon index showed no significant differences among the initial, middle, and last states. Meanwhile, regarding nutrient content, the total phosphorus (TP) concentration decreased by 57.66% at the middle stage and by 56.76% at the last state. Total nitrogen (TN) decreased by 50.86% and 49.30%, respectively. Chemical oxygen demand (COD) decreased by 36.83% and 38.47%, while chlorophyll a (Chla) decreased by 72.36% and 78.54%, respectively. Redundancy analysis (RDA) results indicated that none of the selected environmental variables significantly affected the species community except for conductivity. Our findings suggest that when utilizing multiple species for CWs, it is essential to focus on the well-established species within the plant community. By maintaining these well-established species, water purification in CWs can be sustained. Full article
(This article belongs to the Special Issue Aquatic Plants and Wetland)
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<p>Relative richness of the 20 established species in the three states (<b>a</b>) and the 16 disappeared species in the last state (<b>b</b>) in CW.</p>
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<p>The comparison of Shannon index and the species richness among three stages (initial, middle, and last) in CW (N = 12 plots). The small letter ‘a’ indicated no the differences.</p>
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<p>Nutrient content (TP, TN, COD, and Chla) among three states in CW (N = 12 plots). The small letter ‘a’ and ‘b’ indicated the differences.</p>
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<p>Redundancy analysis (RDA) (scaling = 2) of plant community with selected environmental variables.</p>
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<p>The design and information of the constructed wetland of the polder.</p>
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20 pages, 7316 KiB  
Article
A Diagnostic and Performance System for Soccer: Technical Design and Development
by Alberto Gascón, Álvaro Marco, David Buldain, Javier Alfaro-Santafé, Jose Victor Alfaro-Santafé, Antonio Gómez-Bernal and Roberto Casas
Sports 2025, 13(1), 10; https://doi.org/10.3390/sports13010010 - 8 Jan 2025
Viewed by 325
Abstract
This study presents a novel system for diagnosing and evaluating soccer performance using wearable inertial sensors integrated into players’ insoles. Designed to meet the needs of professional podiatrists and sports practitioners, the system focuses on three key soccer-related movements: passing, shooting, and changes [...] Read more.
This study presents a novel system for diagnosing and evaluating soccer performance using wearable inertial sensors integrated into players’ insoles. Designed to meet the needs of professional podiatrists and sports practitioners, the system focuses on three key soccer-related movements: passing, shooting, and changes of direction (CoDs). The system leverages low-power IMU sensors, Bluetooth Low Energy (BLE) communication, and a cloud-based architecture to enable real-time data analysis and performance feedback. Data were collected from nine professional players from the SD Huesca women’s team during controlled tests, and bespoke algorithms were developed to process kinematic data for precise event detection. Results indicate high accuracy rates for detecting ball-striking events and CoDs, with improvements in algorithm performance achieved through adaptive thresholds and ensemble neural network models. Compared to existing systems, this approach significantly reduces costs and enhances practicality by minimizing the number of sensors required while ensuring real-time evaluation capabilities. However, the study is limited by a small sample size, which restricts generalizability. Future research will aim to expand the dataset, include diverse sports, and integrate additional sensors for broader applications. This system offers a valuable tool for injury prevention, player rehabilitation, and performance optimization in professional soccer, bridging technical advancements with practical applications in sports science. Full article
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<p>System architecture.</p>
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<p>(<b>left</b>) IMU Sensor. (<b>middle</b>) IMU Sensor placed inside the insole. (<b>right</b>) IMU Sensor placed on the instep.</p>
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<p>Cloud APP architecture.</p>
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<p>View of different screens of the mobile APP.</p>
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<p>First part of the sequence diagram for session data register.</p>
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<p>Second part of the sequence diagram for session data register.</p>
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<p>This image shows the different setups of the tests. The green mark indicates the starting point, the red mark indicates the end point and the blue arrows indicate the movement of the ball. The continuous arrows show the movement of the player during the test and the dashed arrows show the return movement to the starting point. (<b>a</b>) Shooting test setup, (<b>b</b>) passing test setup, and (<b>c</b>) change of direction test setup.</p>
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<p>Representation of data for both feet from player 1’s labeled and synchronized change-of-direction test.</p>
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<p>Overview of the shooting test data.</p>
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<p>Example of the process followed by the algorithm to detect shooting events.</p>
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<p>Example of the process followed by the algorithm to detect passing events.</p>
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<p>Diagram of the data preparation process.</p>
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<p>Raw data representation of a change-of-direction test file with eight rounds indicated.</p>
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<p>Representation of the first round of the CoD test and its different parts.</p>
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<p>(<b>a</b>) Confusion matrix of the shooting test. (<b>b</b>) Confusion matrix of the passing test.</p>
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<p>Representation of the true labels, the results of the ensemble model (the raw averaged results in red and the rounded averaged results in green), and the final predictions after filtering out those with insufficient duration.</p>
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<p>Representation of the true labels, the processing made after the ensemble model results (the threshold in green varies since test rounds have been processed separately), and the results obtained after the final filtering process.</p>
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<p>Confusion matrices of the 9 different models generated (E., event; N.E., no event). Diagonal values below 0.5 are shown in red and values below 0.7 are shown in orange.</p>
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25 pages, 4157 KiB  
Article
Textile Wastewater Coupled Treatment Implementing Enhanced Ozonation with Fenton-like Processes and Phytoremediation
by Jazmin A. Reyes-Pérez, Araceli Amaya-Chávez, Gabriela Roa-Morales, Patricia Balderas-Hernández, Thelma B. Pavón Silva, Teresa Torres-Blancas and Carlos E. Barrera-Díaz
Catalysts 2025, 15(1), 43; https://doi.org/10.3390/catal15010043 - 6 Jan 2025
Viewed by 391
Abstract
In this contribution, we described how phytoremediation using M. aquaticum is feasible with coupled ozonation/Fenton-like processes in real wastewater from the denim textile industry, with the purpose of removing color and, therefore, highly polluting particles. For the ozonation/Fenton-like process, pHs of 3 and [...] Read more.
In this contribution, we described how phytoremediation using M. aquaticum is feasible with coupled ozonation/Fenton-like processes in real wastewater from the denim textile industry, with the purpose of removing color and, therefore, highly polluting particles. For the ozonation/Fenton-like process, pHs of 3 and 9 were evaluated using a copper-enriched pumice to activate the catalytic processes carried out in the Fenton-like reactions. Subsequently, phytoremediation was carried out using M. aquaticum to completely degrade the by-products generated from the ozone/Fenton-like process. Plant health was controlled through the determination of chlorophylls and carotenes. All the analyses were monitored by COD, UV–Vis spectrophotometry for the determinations of color quantification in the wastewater and oxidizable organic matter, and SEM/EDS for the characterization of the material and XPS to corroborate the oxidation state of the copper that gives rise to radical species. The results indicate that, at pH 3.0, the ozonation process with the PMPCu catalyst at 1 g/L is the most efficient, achieving a percentage of color removal of 86.79 ± 1.3% and COD of 76.19%, which is consistent with the optimization analysis of the experimental design. The residual color and its degradation by-products were eliminated by phytoremediation. Full article
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<p>Preliminary tests: (<b>a</b>) individual reagent assessment of wastewater color reaching removal percentages of 3.61 ± 0.57, 7.36 ± 1.5, 7.27 ± 2.2, and 81.20 ± 0.96 for PN, POx, PCu, and ozone alone, respectively; and (<b>b</b>) ozone flow assessment in color removal by obtaining the following decreasing order 150 &gt; 100 &gt; 50 mL/min with 84.60 ± 1.5, 60.94 ± 2.2, and 55.71 ± 3.0, respectively.</p>
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<p>Effect of initial pH by evaluating pH 3.0 and pH 9.0 on color removal in industrial wastewater based on ozone time (t-Student <span class="html-italic">p</span> &lt; 0.05).</p>
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<p>(<b>a</b>,<b>c</b>) Percentage of color removal using different materials as catalysts (PN, PCu, and PMPCu) during the ozonation/Fenton-like process at pH 3.0 and pH 9.0, respectively (ANOVA <span class="html-italic">p</span> &gt; 0.05 within each group, and <span class="html-italic">p</span> &lt; 0.05 between groups); and (<b>b</b>,<b>d</b>) color degradation kinetics for pH 3.0 and 9.0 with each type of catalyst.</p>
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<p>(<b>a</b>,<b>c</b>) Percentage of color removal using different materials as catalysts (PN, PCu, and PMPCu) during the ozonation/Fenton-like process at pH 3.0 and pH 9.0, respectively (ANOVA <span class="html-italic">p</span> &gt; 0.05 within each group, and <span class="html-italic">p</span> &lt; 0.05 between groups); and (<b>b</b>,<b>d</b>) color degradation kinetics for pH 3.0 and 9.0 with each type of catalyst.</p>
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<p>Pareto diagram for standardized effects in ozonation/Fenton-like process.</p>
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<p>Color removal percentage using different concentrations of catalysts PMPCu (1, 5, and 7.5 g/L) in the ozonation process at pH 3.0 (ANOVA <span class="html-italic">p</span> &gt; 0.05).</p>
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<p>UV–Vis absorption spectra for color removal of ozone-treated textile wastewater at pH 3.0 with 1 g/L of PMPCu.</p>
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<p>(<b>a</b>) UV–Vis absorption spectra of WTOFL treated with M. aquaticum in phytoremediation systems; and (<b>b</b>) UV–Vis absorption spectra of WTOFL and a standard solution of isatin.</p>
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<p>Effect of the process of phytoremediation in the main photosynthetic pigments assessed in the ratio (<b>a</b>) chlorophylls a/b, and (<b>b</b>) total chlorophylls/carotenes.</p>
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<p>Cyclic voltammogram of (<b>a</b>) textile wastewater prior to treatment, and (<b>b</b>) treated wastewater with ozonation/PMPCu at pH 3.0 and 1000 μL of coagulant added. No support electrolyte was added; sweep speed: 100 mV/s.</p>
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<p>Cyclic residual water voltamperograms (CVs) prior to phytoremediation systems (D0), end-of-phytoremediation water (D14), and plant less water control. No electrolyte support was added; sweep speed: 100 mV/s.</p>
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<p>SEM-EDS micrographs of (<b>a</b>) natural pumice (PN), and (<b>b</b>,<b>c</b>) pumice modified with copper particles (PMPCu) before and after treatment; and (<b>d</b>) EDS analysis.</p>
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<p>(<b>a</b>) PN and PMPCu X-ray diffractograms; and (<b>b</b>) PMPCu XPS spectrum.</p>
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<p>Ozonation system scheme consisting of (a) dry air inlet; (b) ozone generator; (c) flowmeter; (d) vertical flow glazed reactor; (e) sampling valve; and (f) ozone destroyer.</p>
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14 pages, 263 KiB  
Article
Associations Between Dynamic Strength Index and Jumping, Sprinting and Change of Direction Performance in Highly Trained Basketball Players
by Jernej Pleša, Filip Ujaković, Chris Bishop, Nejc Šarabon and Žiga Kozinc
Appl. Sci. 2025, 15(1), 434; https://doi.org/10.3390/app15010434 - 5 Jan 2025
Viewed by 918
Abstract
The aim of this study was to investigate associations and differences between dynamic strength index (DSI) and multi-directional jumping, linear and curvilinear sprinting, and change of direction (CoD). Highly trained basketball players (n = 44) performed a 20 m linear sprint, 20 [...] Read more.
The aim of this study was to investigate associations and differences between dynamic strength index (DSI) and multi-directional jumping, linear and curvilinear sprinting, and change of direction (CoD). Highly trained basketball players (n = 44) performed a 20 m linear sprint, 20 m 3-point line (curvilinear) sprint, countermovement jump (CMJ), drop jump (DJ), bilateral horizontal jump, unilateral horizontal jump, lateral jump, basketball-specific lateral jump and isometric mid-thigh pull (IMTP). The results showed weak to moderate associations between IMTP performance and horizontal jump, lateral jump and curvilinear sprint (r = −0.33–0.41; p < 0.05). No correlations were found between CMJ peak force and performance variables, while weak correlations were observed between DSI and unilateral horizontal jump (r = −0.36; p < 0.05), lateral jumps, linear sprint and CoD deficit (r = −0.37, −0.38; p < 0.05), showing that lower magnitude of DSI is associated with better performance in those tests. Additional analysis revealed that the low DSI subgroup had the highest IMTP peak force, while the high DSI subgroup had the highest CMJ peak force. The low DSI group showed better performance in vertical, horizontal and lateral jumps, while no significant differences were observed in DJ and curvilinear sprint performance compared to other groups. The findings indicate that athletes with lower DSI values exhibit superior physical performance, suggesting that a strength-oriented training approach may be beneficial for basketball players. Due to the ballistic nature of basketball, more maximal strength training is required to optimize the DSI ratio in basketball players. Additional studies are needed to determine the precise benchmarks for navigating training based on DSI values. Full article
(This article belongs to the Special Issue Applied Sports Performance Analysis)
12 pages, 2639 KiB  
Article
Efficacy and Adaptation Mechanisms of Algal-Bacterial Granular Sludge Treatment for Phenolic Wastewater
by Aoxue Yu, Rui Ouyang, Shulian Wang, Bin Ji and Lu Cai
Water 2025, 17(1), 127; https://doi.org/10.3390/w17010127 - 5 Jan 2025
Viewed by 779
Abstract
The ubiquitous presence of phenolic compounds in effluents poses a risk to aquatic organisms and human health. This study investigates the responses of the emerging algal-bacterial granular sludge process in treating phenolic wastewater. The results showed that phenol at 1, 10, and 100 [...] Read more.
The ubiquitous presence of phenolic compounds in effluents poses a risk to aquatic organisms and human health. This study investigates the responses of the emerging algal-bacterial granular sludge process in treating phenolic wastewater. The results showed that phenol at 1, 10, and 100 mg/L had little effect on ammonia-N, chemical oxygen demand (COD), and phosphate-P removal. At the highest phenol concentration of 100 mg/L, the average removal rates of ammonia-N, COD, and phosphate-P were 94.8%, 72.9%, and 83.7%, respectively. The presence of phenol led to a decline in chlorophyll content of the algal-bacterial granular sludge, concurrently resulting in an increase in the abundance of microbial diversity. Algal-bacterial granular sludge exhibited mechanisms such as elevated extracellular polymeric substances (EPSs), superoxide dismutase (SOD), and catalase (CAT) production, which may aid in coping with oxidative stress from phenols. This research underscores the algal-bacterial granular sludge’s potential for treating phenolic wastewater, thereby advancing knowledge in the field of phenol degradation with this innovative technology. Full article
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<p>Morphologies during different culture processes ((<b>a</b>): 0 d; (<b>b</b>): 60 d; (<b>c</b>): 120 d; (<b>d</b>): 150 d) and SEM images (<b>e</b>,<b>f</b>) of algal-bacterial granular sludge.</p>
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<p>Removal profiles of ammonia-N (<b>a</b>), COD (<b>b</b>), and phosphate-P (<b>c</b>) across 60-day operation.</p>
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<p>Microbial community of prokaryotes at phylum level (<b>a</b>) and eukaryotes at species level (<b>b</b>).</p>
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<p>Changes in photosynthetic pigment content.</p>
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<p>Protein (<b>a</b>) and polysaccharide (<b>b</b>) content in EPSs.</p>
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<p>Activities of MDA (<b>a</b>), SOD (<b>b</b>), and CAT (<b>c</b>).</p>
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16 pages, 1366 KiB  
Article
Environmental Sustainability Assessment of pH-Shift Technology for Recovering Proteins from Diverse Fish Solid Side Streams
by Erasmo Cadena, Ozan Kocak, Jo Dewulf, Ingrid Undeland and Mehdi Abdollahi
Sustainability 2025, 17(1), 323; https://doi.org/10.3390/su17010323 - 3 Jan 2025
Viewed by 711
Abstract
The demand for clean-cut seafood fillets has led to an increase in fish processing side streams, which are often considered to be low-value waste despite their potential as a source of high-quality proteins. Valorizing these side streams through innovative methods could significantly enhance [...] Read more.
The demand for clean-cut seafood fillets has led to an increase in fish processing side streams, which are often considered to be low-value waste despite their potential as a source of high-quality proteins. Valorizing these side streams through innovative methods could significantly enhance global food security, reduce environmental impacts, and support circular economy principles. This study evaluates the environmental sustainability of protein recovery from herring, salmon, and cod side streams using pH-shift technology, a method that uses acid or alkaline solubilization followed by isoelectric precipitation to determine its viability as a sustainable alternative to conventional enzymatic hydrolysis. Through a Life Cycle Assessment (LCA), five key environmental impact categories were analyzed: carbon footprint, acidification, freshwater eutrophication, water use, and cumulative energy demand, based on a functional unit of 1 kg of the protein ingredient (80% moisture). The results indicate that sodium hydroxide (NaOH) use is the dominant environmental impact driver across the categories, while energy sourcing also significantly affects outcomes. Compared to conventional fish protein hydrolysate (FPH) production, pH-shift technology achieves substantial reductions in carbon footprint, acidification, and water use, exceeding 95%, highlighting its potential for lower environmental impacts. The sensitivity analyses revealed that renewable energy integration could further enhance sustainability. Conducted at a pilot scale, this study provides crucial insights into optimizing fish side stream processing through pH-shift technology, marking a step toward more sustainable seafood production and reinforcing the value of renewable energy and chemical efficiency in reducing environmental impacts. Future work should address scaling up, valorizing residual fractions, and expanding comparisons with alternative technologies to enhance sustainability and circularity. Full article
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<p>Definition of system boundaries for pH-shift technology for different fish solid side streams.</p>
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<p>Climate change impact category results for protein extraction from solid fish side streams using pH-shift technology.</p>
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<p>Acidification impact category results for protein extraction from solid fish side streams using pH-shift technology.</p>
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<p>Freshwater eutrophication impact category results for protein extraction from solid fish side streams using pH-shift technology.</p>
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<p>Water use impact category results for protein extraction from solid fish side streams using pH-shift technology.</p>
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<p>Cumulative energy demand impact category results for protein extraction from solid fish side streams using pH-shift technology.</p>
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