Different pollutants often coexist in natural environments, making it crucial to monitor and study the ecotoxicological effects of composite pollutants in aquatic environments. Nanoplastics and heavy metals are emerging environmental pollutants that can affect the health of aquatic organisms and threaten human health via the food chain. In this study, zebrafish was employed as a model organism to explore the effects of short-term exposure to polystyrene nanoplastics (PS-NPs) and heavy metal copper ions (Cu
2+) either individually or in combination on fish behavior. First, the single and combined toxicity of Cu
2+ and PS-NPs to adult zebrafish was investigated to obtain the LC
50 values of the two pollutants at 24, 48, 72, and 96 h. Then, the effects of sub-lethal concentrations of Cu
2+ (0.06, 0.15, and 0.3 mg/L), PS-NPs (5, 10, and 15 mg/L) and binary mixtures containing Cu
2+ and PS-NPs (0.06 mg/L + 10 mg/L, 0.15 mg/L + 10 mg/L, and 0.3 mg/L + 10 mg/L) on the swim speed and individual distance of zebrafish within 4 h were studied. The results show that the LC
50 value for single exposure of zebrafish to Cu
2+ decreased with the increase in the exposure time, while PS-NPs showed no significant acute toxicity to zebrafish when the concentration was less than 20 mg/L and the exposure time was less than 96 h. The combined exposure of zebrafish to Cu
2+ and PS-NPs resulted in a 3.1–32.2% reduction in the LC
50 value at different time points compared with Cu
2+ alone. In the behavioral study, both single and combined exposure to Cu
2+ and PS-NPs induced hyperactivity and aggregation phenomena in the zebrafish at different levels; the duration of these two phenomena was correlated with the concentration of the pollutants. The combined exposure to Cu
2+ and PS-NPs exacerbated the behavioral changes in zebrafish compared with exposure to Cu
2+ alone, reducing their hyperactivity time, average swim speed and aggregation time by 30.7–41.0%, 13.6–15.4%, and 28.3–28.8%, respectively. Therefore, this study indicates that the combined short-term exposure to PS-NPs and Cu
2+ can exacerbate the toxicity of pollutants, and also proves the feasibility for early warning of combined NPs and heavy metals pollution based on adult zebrafish behavioral indicators.
Full article