Cryo-EM structure of IrtAB 2xEQ mutant in outward-occluded state in complex with mycobactin
Resolution: 2.67 Å
EM Method: Single-particle
Fitted PDBs: 9fw3
Q-score: 0.594
Gonda I, Sorrentino S, Galazzo L, Lichti NP, Arnold FM, Mehdipour AR, Bordignon E, Seeger MA
Nature (2024) [ DOI: doi:10.1038/s41467-024-55136-7 ]
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Mycobactin import atp-binding/permease protein irtb (61 kDa, Protein from Mycolicibacterium thermoresistibile ATCC 19527)
- Mycobactin s (908 Da, Ligand)
- Mycobactin import atp-binding/permease protein irta (64 kDa, Protein from Mycolicibacterium thermoresistibile ATCC 19527)
- Irtab (126 kDa, Complex from Mycolicibacterium thermoresistibile ATCC 19527)
- Adenosine-5'-triphosphate (507 Da, Ligand)
Cryo-EM structure of IrtAB in inward-facing state in nanodisc
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 9fxc
Q-score: 0.468
Gonda I, Sorrentino S, Galazzo L, Lichti NP, Arnold FM, Mehdipour AR, Bordignon E, Seeger MA
Nature (2024) [ DOI: doi:10.1038/s41467-024-55136-7 ]
- Irtab (158 kDa, Complex from Mycolicibacterium thermoresistibile ATCC 19527)
- Mycobactin import atp-binding/permease protein irta (97 kDa, Protein from Mycolicibacterium thermoresistibile ATCC 19527)
- Mycobactin import atp-binding/permease protein irtb (61 kDa, Protein from Mycolicibacterium thermoresistibile ATCC 19527)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
Cryo-EM structure of IrtAB in outward-occluded state in nanodisc in complex with ADP-vanadate
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 9g2k
Q-score: 0.532
Gonda I, Sorrentino S, Galazzo L, Lichti NP, Arnold FM, Mehdipour AR, Bordignon E, Seeger MA
Nature (2024) [ DOI: doi:10.1038/s41467-024-55136-7 ]
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Adp orthovanadate (544 Da, Ligand)
- Mycobactin import atp-binding/permease protein irtb (61 kDa, Protein from Mycolicibacterium thermoresistibile ATCC 19527)
- Irtab (158 kDa, Complex from Mycolicibacterium thermoresistibile ATCC 19527)
- Mycobactin import atp-binding/permease protein irta (97 kDa, Protein from Mycolicibacterium thermoresistibile ATCC 19527)
Cryo-EM structure of IrtAB in inward-facing state in LMNG
Resolution: 3.23 Å
EM Method: Single-particle
Fitted PDBs: 9g2l
Q-score: 0.489
Gonda I, Sorrentino S, Galazzo L, Lichti NP, Arnold FM, Mehdipour AR, Bordignon E, Seeger MA
Nature (2024) [ DOI: doi:10.1038/s41467-024-55136-7 ]
- Irtab (158 kDa, Complex from Mycolicibacterium thermoresistibile ATCC 19527)
- Zinc ion (65 Da, Ligand)
- Mycobactin import atp-binding/permease protein irta (97 kDa, Protein from Mycolicibacterium thermoresistibile ATCC 19527)
- Mycobactin import atp-binding/permease protein irtb (61 kDa, Protein from Mycolicibacterium thermoresistibile ATCC 19527)
Cryo-EM structure of IrtAB in outward-occluded state in LMNG in complex with ADP-vanadate
Resolution: 3.23 Å
EM Method: Single-particle
Fitted PDBs: 9g2m
Q-score: 0.513
Gonda I, Sorrentino S, Galazzo L, Lichti NP, Arnold FM, Mehdipour AR, Bordignon E, Seeger MA
Nature (2024) [ DOI: doi:10.1038/s41467-024-55136-7 ]
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Adp orthovanadate (544 Da, Ligand)
- Mycobactin import atp-binding/permease protein irtb (61 kDa, Protein from Mycolicibacterium thermoresistibile ATCC 19527)
- Irtab (158 kDa, Complex from Mycolicibacterium thermoresistibile ATCC 19527)
- Mycobactin import atp-binding/permease protein irta (97 kDa, Protein from Mycolicibacterium thermoresistibile ATCC 19527)
Cryo-EM structure of IrtAB 2xEQ mutant in outward-occluded state in nanodisc
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 9g2p
Q-score: 0.64
Gonda I, Sorrentino S, Galazzo L, Lichti NP, Arnold FM, Mehdipour AR, Bordignon E, Seeger MA
Nature (2024) [ DOI: doi:10.1038/s41467-024-55136-7 ]
- Magnesium ion (24 Da, Ligand)
- Irtab (129 kDa, Complex from Mycolicibacterium thermoresistibile ATCC 19527)
- Mycobactin import atp-binding/permease protein irtb (61 kDa, Protein from Mycolicibacterium thermoresistibile ATCC 19527)
- Zinc ion (65 Da, Ligand)
- Mycobactin import atp-binding/permease protein irta (67 kDa, Protein from Mycolicibacterium thermoresistibile ATCC 19527)
- Adenosine-5'-triphosphate (507 Da, Ligand)
Cryo-EM structure of IrtAB 2xEQ, Q249R_IrtB mutant in LMNG
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 9g2s
Q-score: 0.555
Gonda I, Sorrentino S, Galazzo L, Lichti NP, Arnold FM, Mehdipour AR, Bordignon E, Seeger MA
Nature (2024) [ DOI: doi:10.1038/s41467-024-55136-7 ]
- Magnesium ion (24 Da, Ligand)
- Mycobactin import atp-binding/permease protein irta (64 kDa, Protein from Mycolicibacterium thermoresistibile ATCC 19527)
- Mycobactin import atp-binding/permease protein irtb (61 kDa, Protein from Mycolicibacterium thermoresistibile ATCC 19527)
- Irtab (126 kDa, Complex from Mycolicibacterium thermoresistibile ATCC 19527)
- Adenosine-5'-triphosphate (507 Da, Ligand)
Cryo-EM structure of IrtAB 2xEQ, A256R_IrtB mutant in LMNG
Resolution: 3.15 Å
EM Method: Single-particle
Fitted PDBs: 9g2t
Q-score: 0.528
Gonda I, Sorrentino S, Galazzo L, Lichti NP, Arnold FM, Mehdipour AR, Bordignon E, Seeger MA
Nature (2024) [ DOI: doi:10.1038/s41467-024-55136-7 ]
- Magnesium ion (24 Da, Ligand)
- Mycobactin import atp-binding/permease protein irta (64 kDa, Protein from Mycolicibacterium thermoresistibile ATCC 19527)
- Irtab (126 kDa, Complex from Mycolicibacterium thermoresistibile ATCC 19527)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Mycobactin import atp-binding/permease protein irtb (61 kDa, Protein from Mycolicibacterium thermoresistibile ATCC 19527)
Resolution: 3.35 Å
EM Method: Single-particle
Fitted PDBs: 9g2v
Q-score: 0.394
Gonda I, Sorrentino S, Galazzo L, Lichti NP, Arnold FM, Mehdipour AR, Bordignon E, Seeger MA
Nature (2024) [ DOI: doi:10.1038/s41467-024-55136-7 ]
- Zinc ion (65 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Irtab (126 kDa, Complex from Mycolicibacterium thermoresistibile ATCC 19527)
- Mycobactin import atp-binding/permease protein irtb (61 kDa, Protein from Mycolicibacterium thermoresistibile ATCC 19527)
- Mycobactin import atp-binding/permease protein irta (64 kDa, Protein from Mycolicibacterium thermoresistibile ATCC 19527)
Resolution: 2.85 Å
EM Method: Single-particle
Fitted PDBs: 9g2x
Q-score: 0.546
Gonda I, Sorrentino S, Galazzo L, Lichti NP, Arnold FM, Mehdipour AR, Bordignon E, Seeger MA
Nature (2024) [ DOI: doi:10.1038/s41467-024-55136-7 ]
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Irtab (126 kDa, Complex from Mycolicibacterium thermoresistibile ATCC 19527)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Mycobactin import atp-binding/permease protein irtb (61 kDa, Protein from Mycolicibacterium thermoresistibile ATCC 19527)
- Mycobactin import atp-binding/permease protein irta (64 kDa, Protein from Mycolicibacterium thermoresistibile ATCC 19527)
- Water (18 Da, Ligand)