Veterinární medicína, 2022 (vol. 67), issue 1
Roles of apoptosis and autophagy in natural rabies infectionsOriginal Paper
M Ozkaraca, S Ozdemir, S Comakli, MO Timurkan
Vet Med - Czech, 2022, 67(1):1-12 | DOI: 10.17221/221/2020-VETMED
The aim of this study was to investigate the activity of apoptosis and autophagy in animals (cows, horses, donkeys, dogs and cats) naturally infected with rabies by using immunohistochemistry, immunofluorescence, and qPCR. The mRNA transcript levels of caspase-3, Bax, Bcl2 and LC3B were determined with qPCR. Caspase-3 and AIF immunopositivity were not observed in the immunohistochemical and immunofluorescence staining, whereas LC3B immunopositivity was determined intensively in the infected animals compared to the control groups. LC3B immunopositivity was detected in the cytoplasm of the Purkinje cells in the cerebellum...
Serological prevalence of Leptospira serovars among pigs in Ukraine during the period of 2001-2019Original Paper
V Ukhovskyi, A Pyskun, L Korniienko, H Aliekseieva, O Moroz, O Pyskun, G Kyivska, A Mezhenskyi
Vet Med - Czech, 2022, 67(1):13-27 | DOI: 10.17221/50/2021-VETMED
Leptospirosis is a widespread infection among pigs throughout the world. In most cases in Ukraine, only the microscopic agglutination test (MAT) is used for the diagnosis of leptospirosis in animals. In general, during the period of 2001-2019, 2 381 163 samples of blood sera from swine were tested in our country and 85 338 positive reactions were received, which is 3.58% [binomial confidence intervals (BCI), 3.56-3.61%]. It was established that the serovars copenhageni - 33.91% (BCI, 33.59-34.23%), bratislava - 14.14% (BCI, 13.90-14.37%), pomona - 8.58% (BCI, 8.39-8.77%), and tarassovi - 7.12% (BCI, 6.95-7.30%) play...
Effect of Bacillus subtilis on the microarchitectural development of the immune system in Salmonella-challenged broiler chickensOriginal Paper
A Sikandar, H Zaneb, A Nasir, A Rehman, M Kashif, M Shah, Z Luqman, S Din, MF Iqbal, I Khan, I Irshad
Vet Med - Czech, 2022, 67(1):28-37 | DOI: 10.17221/231/2020-VETMED
The effect of Bacillus subtilis on the immune responses and morphometry of the immune organs was evaluated in broilers challenged with S. gallinarum. For this purpose, Salmonella-free birds (n = 240) were split into four groups with six replicates of ten birds each. Groups included an NC (negative control, non-infected + non-medicated), a PC-S (positive control, Salmonella-infected + non-medicated), an AT-S (Salmonella-infected + medicated with enrofloxacin), and a BS-S (Salmonella-infected + B. subtilis (2.0 × 1010 cfu/g; 0.1 g/kg) group. On day 21, the thickness of...
Autologous adipose-derived mesenchymal stem cells and hydroxyapatite for bone defect in rabbitsOriginal Paper
GG Franco, BW Minto, LP Coelho, PF Malard, ER Carvalho, FYK Kawamoto, BM Alcantara, LGGG Dias
Vet Med - Czech, 2022, 67(1):38-45 | DOI: 10.17221/85/2020-VETMED
This study aims to evaluate the effect of autologous adipose-derived mesenchymal stem cells (AAD-MSC), with and without synthetic absorbable hydroxyapatite (HAP-91), on the bone regeneration in rabbits. Thirty-four female white New Zealand rabbits were submitted to a 10 mm distal diaphyseal radius ostectomy, divided into 3 experimental groups according to the treatment established. The bone gap was filled with 0.15 ml of a 0.9% saline solution containing two million AAD-MSC (G1), or AAD-MSC associated with HAP-91 (G2). The control group (CG) received only 0.15 ml of the 0.9% saline solution. Radiographs were made post-operatively, and after 15, 30,...
A case report: Characteristic plain radiographic findings of a displaced abomasum in a heiferCase Report
K Kazama, K Onda, S Arai, Y Shinozuka, K Kawai, K Kaneko, T Kondo, K Yamada
Vet Med - Czech, 2022, 67(1):46-50 | DOI: 10.17221/83/2021-VETMED
A 9-month-old Holstein heifer with a history of severely poor growth presented with diarrhoea. On physical examination, a metallic pinging sound was heard on the simultaneous percussion and auscultation of the left trunk. On the cranial abdominal radiography, the contour of a gas-filled balloon-like abomasum wall was delineated, which elevated to the dorsal abdomen. Radiopaque sand at the bottom of the abomasum had been pulled up caudodorsally by the gas-filled abomasum. After surgery, the gas-filled balloon-like appearance of the abomasum wall disappeared and the radiopaque sand was located in the normal position. To our knowledge, no reports on a...
Congenital hydrocephalus in three sheep: Clinical, electroencephalographic and pathological featuresCase Report
M Masucci, MT Capucchio, R Buttitta, E Colombino, SA Mignacca
Vet Med - Czech, 2022, 67(1):51-58 | DOI: 10.17221/8/2021-VETMED
The clinical, electroencephalographic and neuro-pathological features of three cases (cases 1, 2 and 3) of congenital hydrocephalus in sheep were described. The observed neurological signs reflected damage in the telencephalon and brain stem. The electroencephalogram performed in case 1 and case 2 showed different patterns: symmetric and synchronous high-voltage slow-activity in case 1, and low-voltage slow-activity in case 2. By the post-mortem examination, in all the animals, dilatation of the ventricular system, especially of the lateral ventricles, associated with a glial reaction surrounding the dilated ventricles was observed. Only in case 3,...
Corrigendum: Developmental competence of mammalian oocytes-insights into molecular research and the promise of microfluidic technology: A reviewCorrigendum
B Kempisty, P Zawierucha, S Ciesiolka, H Piotrowska, P Antosik, D Bukowska, M Jeseta, M Nowicki, KP Brussow, M Zabel
Vet Med - Czech, 2022, 67(1):59 | DOI: 10.17221/13961-VETMED