The 353d Bombardment Squadron is an inactive United States Air Force unit. It last was assigned to the 301st Bombardment Wing, stationed at Lockbourne Air Force Base, Ohio. It was inactivated on 8 June 1964. During World War II, the 353d Bombardment Squadron was a B-17 Flying Fortress squadron, assigned to the 301st Bombardment Group, Fifteenth Air Force. It earned two Distinguished Unit Citations.