Abstract
The generation of distinct cell types during development depends on the competence of progenitor populations to differentiate along specific lineages. Here we investigate the mechanisms that regulate competence of rodent cortical progenitors to differentiate into astrocytes in response to ciliary neurotrophic factor (CNTF). We found that fibroblast growth factor 2 (FGF2), which by itself does not induce astrocyte-specific gene expression, regulates the ability of CNTF to induce expression of glial fibrillary acidic protein (GFAP). FGF2 facilitates access of the STAT/CBP (signal transducer and activator of transcription/CRE binding protein) complex to the GFAP promoter by inducing Lys4 methylation and suppressing Lys9 methylation of histone H3 at the STAT binding site. Histone methylation at this site is specific to the cell's state of differentiation. In progenitors, the promoter is bound by Lys9-methylated histones, and in astrocytes, it is bound by Lys4-methylated histones, indicating that astrocyte differentiation in vivo involves this switch in chromatin state. Our observations indicate that extracellular signals can regulate access of transcription factors to genomic promoters by local chromatin modification, and thereby regulate developmental competence.
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Acknowledgements
We thank G. Nolan for the retroviral vectors, S. Temple for advice on cell sorting, K. Chadwick for help with FACS, D. Reinberg and Y. Zhang for SET7/9 constructs, and B. Barres, S. Pfaff, D. Ginty, P. Beachy and M. Greenberg for discussion. This work was supported by National Institutes of Health grant NS36176 (A.G.), the March of Dimes Birth Defects Foundation (A.G.), a Pew Scholar Award (A.G.) and the Johns Hopkins Center for AIDS Research 1P30AI42855 (K.C.).
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Supplementary information
Supplementary Fig. 1
Effect of transient FGF2 exposure on cell proliferation in glial clones. E18 cultures were infected with GFP retrovirus and were exposed to FGF2 for indicated duration on day 1 or continuously for 5 days. The number of cells in glial clones was scored on day 5. Note that brief stimulation of FGF2 did not promote proliferation compared to the control. Asterisks indicate statistically significant differences by paired t test (P<0.01 for **) between the control and the indicated experimental group. (JPG 22 kb)
Supplementary Fig. 2
Effect of FGF2 on DNA methylation at the GFAP promoter. Genomic DNA was prepared from E15 rat cortical cells either acutely or after culture for 5 days with or without FGF2 treatments. DNA methylation level was assessed by bisulfite sequencing method. In brief, 10 ug of genomic DNA was prepared, sonicated, and denatured with 0.3M NaOH at 37°C for 20 min. After incubation with 3.1 M sodium bisulfite and 0.5 mM hydroquinone at 55°C for 16 hours, DNA samples were purified with a desalting column (Qiagen). Eluted samples were denatured by NaOH at 37°C for 20 min, neutralized by ammonium acetate, and recovered by ethanol precipitation. PCR was performed using following primers to amplify STAT binding-site sequence: GFmS(ttaatttttttaggatttttttttttgtgttt) and GfmAS ( aatatctactccaaaacatttactaaataaaa). PCR products were cloned using PCR cloning kit (Invitrogen) and transformed into bacteria. 10 to 15 clones were randomly picked in each group of three independent PCRs and sequenced. (JPG 22 kb)
Supplementary Fig. 3
Effect of FGF2 on overall histone methylation at lysine-4. Cells were treated with FGF2 for 24 hours and whole cell extracts were immunoblotted with anti-dimethyl histone H3 (lysine-4) or histone H3. (JPG 12 kb)
Supplementary Fig. 4
Effect of SET7/9 expression on the proportion of neuronal clones. Cells were retrovirally infected to misexpress SET7/9 and were grown for 5 DIV. Clonal analysis was performed based on GFP and β-tubulin III immunofluorescence. (JPG 29 kb)
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Song, MR., Ghosh, A. FGF2-induced chromatin remodeling regulates CNTF-mediated gene expression and astrocyte differentiation. Nat Neurosci 7, 229–235 (2004). https://doi.org/10.1038/nn1192
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DOI: https://doi.org/10.1038/nn1192