Down-regulation of lactate dehydrogenase-A by siRNAs for reduced lactic acid formation of Chinese hamster ovary cells producing thrombopoietin

• Baumgart E, Fahimi HD, Stich A, Volki A (1996) L-lactate dehydrogenase A4-and A3B isoforms are bona fide peroxisomal enzymes in rate liver: evidence for involvement in intraperoxisomal NADH reoxidation. J Biol Chem 271:3846–3855

  Article  CAS  Google Scholar 

• Bertrand JR, Pottier M, Vekris A, Opolon P, Maksimemko A, Malvy C (2002) Comparison of antisense oligonucleotides and siRNAs in cell culture and in vivo. Biochem Biophys Res Commun 296:1000–1004

  Article  CAS  Google Scholar 

• Brummelkamp TR, Bernards R, Agami R (2002) A system for stable expression of short interfering RNAs in mammalian cells. Science 296:550–553

  Article  CAS  Google Scholar 

• Cavalie H, Mounier R, Clottes E, Bricout V, Lac G (2004) Clenbuterol treatment differently affects muscle in exercised or sedentary rats. J Exerc Physiol 7:111–120

  Google Scholar 

• Chen K, Liu Q, Xie L, Sharp PA, Wang DIC (2001) Engineering of a mammalian cell line for reduction of lactate formation and high monoclonal antibody production. Biotechnol Bioeng 72:55–61

  Article  CAS  Google Scholar 

• Chung JY, Ahn HK, Lim SW, Sung YH, Koh YW, Park SK, Lee GM (2003) Development of recombinant Chinese hamster ovary cell lines producing human thrombopoietin or its analog. J Microbiol Biotechnol 13:759–766

  CAS  Google Scholar 

• Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391:806–811

  Article  CAS  Google Scholar 

• Fogolin MB, Wagner R, Etcheverrigaray M, Kratje R (2004) Impact of temperature reduction and expression of yeast puruvate carboxylase on hGM-CSF-producing CHO cells. J Biotechnol 109:179–191

  Article  CAS  Google Scholar 

• Holen T, Amarzguioui M, Wiiger MT, Babaie E, Prydz H (2002) Positional effects of short interfering RNAs targeting the human coagulation trigger Tissue Factor. Nucleic Acids Res 30:1757–1766

  Article  CAS  Google Scholar 

• Hough SR, Wiederholt KA, Burrier AC, Woolf TM, Taylor MF (2003) Why RNAi makes sense. Nat Biotechnol 21:731–732

  Article  CAS  Google Scholar 

• Irani N, Wirth M, van Den Heuvel J, Wagner R (1999) Improvement of the primary metabolism of cell cultures by introducing a new cytoplasmic pyruvate carboxylase reaction. Biotechnol Bioeng 66:238–246

  Article  CAS  Google Scholar 

• Jeong DW, Kim TS, Lee JW, Kim KT, Kim HJ, Kim IH, Kim IY (2001) Blocking of acidosis-mediated apoptosis by a reduction of lactate dehydrogenase activity through antisense mRNA expression. Biochem Biophys Res Commun 289:1141–1149

  Article  CAS  Google Scholar 

• Jiang Z, Huang Y, Sharfstein ST (2006) Regulation of recombinant monoclonal antibody production in Chinese hamster ovary cells: a comparative study of gene copy number, mRNA level, and protein expression. Biotechnol Prog 22:313–318

  Article  CAS  Google Scholar 

• Kim NS, Kim SJ, Lee GM (1998) Clonal variability within dihydrofolate reductase-mediated gene amplified Chinese hamster ovary cells: stability in the absence of selective pressure. Biotechnol Bioeng 60:679–688

  Article  CAS  Google Scholar 

• Kitaura T, Tsunekawa N, Hatta H (2001) Decreased monocarboxylate transporter 1 in rat soleus and EDL muscles exposed to clenbuterol. J Appl Physiol 91:85–90

  Article  CAS  Google Scholar 

• Lao MS, Toth D (1997) Effects of ammonium and lactate on growth and metabolism of a recombinant Chinese hamster ovary cell culture. Biotechnol Prog 13:688–691

  Article  CAS  Google Scholar 

• Li SS-L, Fitch WM, Pan Y-CE, Sharief FS (1983) Evolutionary relationship of vertebrate lactate dehydrogenase isozymes A4 (muscle), B4 (heart) and C4 (tetis). Biol Chem 258:7029–7032

  CAS  Google Scholar 

• Neermann J, Wagner R (1996) Comparative analysis of glucose and glutamine metabolism in transformed mammalian cell lines, insect and primary liver cells. J Cell Physiol 166:152–169

  Article  CAS  Google Scholar 

• Ozturk SS, Palsson BO (1990) Chemical decomposition of glutamine in cell culture media: effect of media type, pH, and serum concentration. Biotechnol Prog 6:121–128

  Article  CAS  Google Scholar 

• Renard JM, Spagnoli R, Mazier C, Salles MF, Mandine E (1988) Evidence that monoclonal antibody production kinetics is related to the integral of viable cells in batch systems. Biotechnol Lett 10:91–96

  Article  Google Scholar 

• Song E, Lee SK, Wang J, Ince N, Ouyang N, Min J, Chen J, Shankar P, Lieberman J (2003) RNA interference targeting Fas protects mice from fulminant hepatitis. Nat Med 9:347–351

  Article  CAS  Google Scholar 

• Sung YH, Hwang SJ, Lee GM (2005) Influence of down-regulation of caspase-3 by siRNAs on sodium butyrate-induced apoptotic cell death of Chinese hamster ovary cells producing thrombopoietin. Metab Eng 7:457–466

  Article  CAS  Google Scholar 

• Tritsch GL, Moore GE (1962) Spontaneous decomposition of glutamine in cell culture media. Exp Cell Res 28:360–364

  Article  CAS  Google Scholar 

• Wong NSC, Yap MGS, Wang DIC (2006) Enhancing recombinant glycoprotein sialylation through CMP-sialic acid transporter over expression in Chinese hamster ovary cells. Biotechnol Bioeng 93:1005–1016

  Article  CAS  Google Scholar 

• Yoon SK, Choi SL, Song JY, Lee GM (2005) Effect of culture pH on erythropoietin production by Chinese hamster ovary cells grown in suspension at 32.5 and 37.0°C. Biotechnol Bioeng 89:345–356

  Article  CAS  Google Scholar 

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