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Expression, tissue distribution and subcellular localization of dehydrin TAS14 in salt-stressed tomato plants

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Abstract

We previously isolated and characterized TAS14, an mRNA that is induced in tomato upon osmotic stress or abscisic acid (ABA) treatment and that shares expression and sequence characteristics with other dehydrin genes in different species. Affinity-purified antibodies against TAS14 protein were used to study the expression of TAS14 protein, both in seedlings and mature plants, its tissue distribution and its subcellular localization. TAS14 protein was not detected in 4-day-old seedlings but accumulated after ABA, NaCl or mannitol treatments. In NaCl-treated seedlings, some protein was detectable after 6 h of treatment and reached maximal levels between 24 and 48 h. Concentrations ranging from 5 to 12.5 g/l NaCl induced the protein to similar levels. In salt-stressed mature plants, TAS14 was expressed abundantly and continuously in aerial parts, but only slightly and transiently in roots. Immunocytochemical analysis of salt-treated plants showed TAS14 accumulated in adventitious root primordia and associated to the provascular and vascular tissues in stems and leaves. Immunogold electron microscopy localized TAS14 protein both in the cytosol and in the nucleus, associated to the nucleolus and euchromatin. Since TAS14 is a phosphoprotein in vivo, the classes of protein kinases potentially responsible for its in vivo phosphorylation were tested in in vitro phosphorylation assays. TAS14 protein was phosphorylated in vitro by both casein kinase II and cAMP-dependent protein kinase.

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Authors and Affiliations

  1. Instituto de Recursos Naturales y Agrobiologia, C.S.I.C., Apartado 1052, 41080, Sevilla, Spain

    José A. Godoy, Sonia Torres-Schumann, Javier Moreno & José A. Pintor-Toro

  2. Departmento de Biología Celular, Facultad de Biología, Universidad de Sevilla, 41012, Sevilla, Spain

    Rosa M. Rodrigo

Authors
  1. José A. Godoy
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  2. Rosa Lunar
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  3. Sonia Torres-Schumann
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  4. Javier Moreno
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  5. Rosa M. Rodrigo
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  6. José A. Pintor-Toro
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The first two authors contributed equally to this paper.

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Godoy, J.A., Lunar, R., Torres-Schumann, S. et al. Expression, tissue distribution and subcellular localization of dehydrin TAS14 in salt-stressed tomato plants. Plant Mol Biol 26, 1921–1934 (1994). https://doi.org/10.1007/BF00019503

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  • DOI: https://doi.org/10.1007/BF00019503

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