Abstract
Protein profiling through affinity proteomic approaches represents a powerful strategy for the analysis of human body fluids. Cerebrospinal fluid (CSF), being the fluid proximal to the central nervous system, is commonly analyzed in the context of neurological diseases, and can offer novel insights into the physiological state of the brain. Ultimately, and by analyzing the presence of brain-derived proteins in larger sets of samples that represent different phenotypes, profiling of CSF may serve as an important source to discover and verify disease-associated markers. Here, we describe a multiplexed and flexible protein profiling approach using antibody-based assays on suspension bead arrays. Through streamlined sample processing, protein biotinylation, and single-binder assay readout, this method enables high-throughput neuroproteomic analysis of up to 384 proteins in 384 samples.
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Acknowledgments
We like to thank the entire staff of the Human Protein Atlas (HPA) for their efforts. This work was funded by the PRONOVA project (VINNOVA, Swedish Governmental Agency for Innovation Systems), and by grants from the Knut and Alice Wallenberg Foundation and Science for Life Laboratory, Stockholm.
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Häggmark-Månberg, A., Nilsson, P., Schwenk, J.M. (2017). Neuroproteomic Profiling of Cerebrospinal Fluid (CSF) by Multiplexed Affinity Arrays. In: Kobeissy, F., Stevens, Jr., S. (eds) Neuroproteomics. Methods in Molecular Biology, vol 1598. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6952-4_11
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DOI: https://doi.org/10.1007/978-1-4939-6952-4_11
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