The isolation of natural products from plant or animal sources poses numerous problems.
The compounds being sought, biologically active or otherwise, may only be present in …

The practice of preparative high-performance liquid chromatography (PLC) is reviewed.
Special attention is paid to problems with the use of this method in research and …

Publisher Summary This chapter presents the strategies for successful preparative liquid
chromatography. Chromatography is a dynamic equilibrium process. At the molecular level …

A method for the enrichment and separation of compounds, called interval
injection/displacement reversed-phase high-performance liquid chromatography (HPLC) …

The purpose of this chapter is not to provide an introduction to HPLC, amply covered in a
number of excellent books (eg Snyder and Kirkland 1979, Simpson 1982, Johnson and …

In organic synthesis, the purification of reactional intermediates or final products is generally
carried out by normal phase flash chromatography. However, for dihydrodipyridopyrazines …

The effect of the concentration of a solution introduced into a column of the solvent, the
capacity factor (k′) and other parameters on the yield of preparative liquid chromatography …

Sample solvents of weaker eluent strength than the mobile phase reduce band broadening
induced by simultaneous mass and volume overload; but increase elution times of the …

The influence of flow velocity on plate height is studied in the range from analytical load up
to 10 mg/g on a normal phase system. By increasing the sample load up to 5 or 10 mg/g, the …

After an engagement in analytical chemistry during 50 years, I am looking back to my
scientific carreer. To some extent it is based on my successful textbook in high-performance …