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Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium.
Acton TB, Gunsalus KC, Xiao R, Ma LC, Aramini J, Baran MC, Chiang YW, Climent T, Cooper B, Denissova NG, Douglas SM, Everett JK, Ho CK, Macapagal D, Rajan PK, Shastry R, Shih LY, Swapna GV, Wilson M, Wu M, Gerstein M, Inouye M, Hunt JF, Montelione GT. Acton TB, et al. Among authors: hunt jf. Methods Enzymol. 2005;394:210-43. doi: 10.1016/S0076-6879(05)94008-1. Methods Enzymol. 2005. PMID: 15808222
Comparisons of NMR spectral quality and success in crystallization demonstrate that NMR and X-ray crystallography are complementary methods for small protein structure determination.
Snyder DA, Chen Y, Denissova NG, Acton T, Aramini JM, Ciano M, Karlin R, Liu J, Manor P, Rajan PA, Rossi P, Swapna GV, Xiao R, Rost B, Hunt J, Montelione GT. Snyder DA, et al. J Am Chem Soc. 2005 Nov 30;127(47):16505-11. doi: 10.1021/ja053564h. J Am Chem Soc. 2005. PMID: 16305237
Crystal structures of two bacterial 3-hydroxy-3-methylglutaryl-CoA lyases suggest a common catalytic mechanism among a family of TIM barrel metalloenzymes cleaving carbon-carbon bonds.
Forouhar F, Hussain M, Farid R, Benach J, Abashidze M, Edstrom WC, Vorobiev SM, Xiao R, Acton TB, Fu Z, Kim JJ, Miziorko HM, Montelione GT, Hunt JF. Forouhar F, et al. Among authors: hunt jf. J Biol Chem. 2006 Mar 17;281(11):7533-45. doi: 10.1074/jbc.M507996200. Epub 2005 Dec 5. J Biol Chem. 2006. PMID: 16330546 Free article.
Protein chaperones Q8ZP25_SALTY from Salmonella typhimurium and HYAE_ECOLI from Escherichia coli exhibit thioredoxin-like structures despite lack of canonical thioredoxin active site sequence motif.
Parish D, Benach J, Liu G, Singarapu KK, Xiao R, Acton T, Su M, Bansal S, Prestegard JH, Hunt J, Montelione GT, Szyperski T. Parish D, et al. J Struct Funct Genomics. 2008 Dec;9(1-4):41-9. doi: 10.1007/s10969-008-9050-y. Epub 2008 Nov 26. J Struct Funct Genomics. 2008. PMID: 19039680 Free PMC article.
Understanding the physical properties that control protein crystallization by analysis of large-scale experimental data.
Price WN 2nd, Chen Y, Handelman SK, Neely H, Manor P, Karlin R, Nair R, Liu J, Baran M, Everett J, Tong SN, Forouhar F, Swaminathan SS, Acton T, Xiao R, Luft JR, Lauricella A, DeTitta GT, Rost B, Montelione GT, Hunt JF. Price WN 2nd, et al. Among authors: hunt jf. Nat Biotechnol. 2009 Jan;27(1):51-7. doi: 10.1038/nbt.1514. Nat Biotechnol. 2009. PMID: 19079241 Free PMC article.
165 results