Fig 2.
PALB2 directly interacts with the KC domain of KEAP1. (A) Schematic of the KEAP1 constructs. (B) In vitro translations were performed to produce HA-tagged KEAP1 proteins (lanes 1 to 4) and FLAG-HA-PALB2 (lane 5). Subsequently, each of the KEAP1 species was mixed with FLAG-HA-PALB2 and an anti-PALB2 antibody was used to precipitate PALB2. Then, the precipitated materials were analyzed by Western blotting using an anti-HA antibody. (C) Various KEAP1 constructs shown in panel A were transfected into 293T cells, and proteins were IPed by anti-FLAG M2 agarose. The precipitates were analyzed by Western blotting.