Figure 5. Manipulating the Clock in the PDF(+) s-LNvs Alters the Clock in the DN1s but Not the Other E-cell Subsets.
(A and B) Maximum projections of confocal sections taken in representative adult UAS-CK2βRNAi/+ and Pdf-GAL4,UAS-CK2βRNAi/+ brains labeled with with PER and PDF antibodies. Sections contain the LNs at CT1, 7, 14, and 20. The PDF(+) s-LNvs and LNds are in boxes, while the 5th s-LNv is indicated by line. (B) Maximum projections of confocal sections taken in representative adult UAS-CK2βRNAi/+ and Pdf-GAL4,UAS-CK2βRNAi/+ brains labeled with PER antibody. Sections contain the DN1s at CT1, 7, 14, and 20. (C) Plots of average normalized pixel intensity versus circadian time for each pacemaker cell group. See experimental procedures for details of quantification method. Error bars represent standard error of mean. The results are a combination of three independent experiments: s-LNv, n=32-76; LNd, n=54-94; 5th s-LNv, n=10-18; DN1, n=104-189. Asterisks mark significant differences between genotypes (Student's t-test, *p<0.05, **p<0.01, ***p<0.001). See also figures S4 and S5 and table S2 for additional data.