(A) Maximum projections of confocal sections taken in representative adult Pdfrhan5304;;UAS-Pdfr/+ and Pdfrhan5304;;Clk4.1M-GAL4/UAS-Pdfr brains labeled with PER antibody. Sections contain the DN1s at CT1, 7, 12, and 18. (B) Maximum projections of confocal sections taken in representative adult Pdfrhan5304;;UAS-Pdfr/+ and Pdfrhan5304;;Clk4.1M-GAL4/UAS-Pdfr brains labeled with with PER and PDF antibodies. Sections contain the LNs at CT1, 7, 12, and 18. The PDF(+) s-LNvs and LNds are in boxes, while the 5th s-LNv is indicated by line. (C) Plots of average normalized pixel intensity versus circadian time for each pacemaker cell group. See experimental procedures for details of quantification method. Error bars represent standard error of mean. The results are a combination of two independent experiments: s-LNv, n=32-57; LNd, n=56-91; 5th s-LNv, n=8-17; DN1, n=143-226. Asterisks mark significant differences between genotypes (Student's t-test, *p<0.05, **p<0.01, ***p<0.001).