Fig. 2.
NIR stimulation of MWCNTs induces thermoablative temperatures that inhibit clonogenic survival of cultured kidney cancer cells. RENCA cells in a final volume of 300 μl of medium were either treated with 100 μg/ml MWCNT or left untreated. Approximately 15 min after the addition of MWCNT, cells were exposed to 30 sec or 45 sec of NIR laser illumination (3 W/cm2). Temperature (two curves, right-hand scale) and clonogenic survival (bars, left-hand scale) were measured. Shown are means and standard deviations of triplicate measurements. (No colonies formed from cells treated with MWCNTs and 45 sec of laser exposure.) The combination of MWCNTs and 30 sec of laser exposure reduced viability 62-fold, and 0 colonies formed from cells treated with both MWCNTs and 45 sec of NIR laser illumination (P ≤ 0.02).