Figure 7.
Effect of depletion of LRP130 in the hepatic fasting response in vivo. (A) Relative gene expression of several genes involved in energy homeostasis. Asterisks (*) indicate that statistical comparisons are made between siControl and siLRP130 in fasted animals. Number signs (#) indicate that statistical comparisons are made between siControl and siLRP130 in fed animals. (*) p < 0.05; (**) p < 0.01; (***) p < 0.001; (#) p < 0.05. Error bars represent standard deviation. (B) Comparison of glucose levels at 0, 12, and 24 h. (C) Comparison of hepatic glucose output as assessed by pyruvate tolerance test following a 16-h fast. (D) Immunoblot for AMPK, phosphorylated-AMPK (p-AMPK), and phosphorylated-acetyl CoA carboxylase (p-ACC) from the fasted animals analyzed in A. Two representative animals are shown.