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. 2006 Nov 1;20(21):2996–3009. doi: 10.1101/gad.1483906

Figure 2.

Figure 2.

Physical interaction between LRP130 and PGC-1α. (A) Wild-type and mutant LRP130 were 35S-labeled by in vitro translation and incubated with purified Flag-tagged PGC-1α. (B) Truncations of LRP130 were 35S-labeled by in vitro translation and incubated with purified Flag-tagged PGC-1α. Anti-Flag matrix was used to immunopurify Flag-tagged PGC-1α protein bound to LRP130. (C) Truncations of PGC-1α or full-length PGC-1β were 35S-labeled and incubated with full-length LRP130. (D) PGC-1β was 35S-labeled and incubated with full-length LRP130. (E) Schematic of LRP130 and PGC-1α indicating regions of interaction.