Figure 4.
Coimmunoprecipitation of various Hop1 mutant proteins with Red1. A. hop1::LEU2 (DW10::YIp5), HOP1 (DW10::pLT11), hop1-585Δ (DW10::pLT11-585), hop1-564Δ (DW10::pLT11-564), hop1-K590A (DW10::pLT11-K590A), and hop1-K593A (DW10::pLT11-K593A) diploids were sporulated for 3 h at 30°C and soluble yeast extracts used for immunoprecipitation with either α-Hop1 or G/R (α-Red1) antibodies. The IPs were fractionated by SDS-PAGE using 6% gels and probed with either α-Hop1 or G/R antibodies as indicated. (B) Red1 was IPed from extracts derived from RED1 (YTS1::pTS30-Q241G) and red1::LEU2 (YTS3) diploids using G/R antibodies. The Red1 IPs were then probed with either G/R or Hop1 antibodies to detect Red1 or Hop1, respectively.