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. 1981 May;314:281–294. doi: 10.1113/jphysiol.1981.sp013707

Electrophysiological analysis of mitral cells in the isolated turtle olfactory bulb.

K Mori, M C Nowycky, G M Shepherd
PMCID: PMC1249433  PMID: 7310692

Abstract

1. An in vitro preparation of the turtle olfactory bulb has been developed. Electrophysiological properties of mitral cells in the isolated bulb have been analysed with intracellular recordings. 2. Mitral cells have been driven antidromically from the lateral olfactory tract, or activated directly by current injection. Intracellular injections of horseradish peroxidase (HRP) show that turtle mitral cells have long secondary dendrites that extend up to 1800 micrometer from the cell body and reach around half of the bulbar circumference. There are characteristically two primary dendrites, each supplying separate olfactory glomeruli. 3. Using intracellular current pulses, the whole-neurone resistance was found to range from 33 to 107 M omega. The whole-neurone charging transient had a slow time course. The membrane time constant was estimated to be 24-93 msec by the methods of Rall. The electrotonic length of the mitral cell equivalent cylinder was estimated by Rall's methods to be 0.9-1.9. 4. The spikes generated by turtle mitral cells were only partially blocked by tetrodotoxin (TTX) in the bathing medium. The TTX-resistant spikes were enhanced in the presence of tetraethylammonium (TEA), and blocked completely by cobalt. 5. The implications of the electrical properties for impulse generation in turtle mitral cells are discussed. The mitral cells have dendrodendritic synapses onto granule cells, and the TTX-resistant spikes may therefore play an important role in presynaptic transmitter release at these synapses.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams D. J., Smith S. J., Thompson S. H. Ionic currents in molluscan soma. Annu Rev Neurosci. 1980;3:141–167. doi: 10.1146/annurev.ne.03.030180.001041. [DOI] [PubMed] [Google Scholar]
  2. Barrett E. F., Barret J. N. Separation of two voltage-sensitive potassium currents, and demonstration of a tetrodotoxin-resistant calcium current in frog motoneurones. J Physiol. 1976 Mar;255(3):737–774. doi: 10.1113/jphysiol.1976.sp011306. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Getchell T. V., Shepherd G. M. Synaptic actions on mitral and tufted cells elicited by olfactory nerve volleys in the rabbit. J Physiol. 1975 Oct;251(2):497–522. doi: 10.1113/jphysiol.1975.sp011105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jackowski A., Parnavelas J. G., Lieberman A. R. The reciprocal synapse in the external plexiform layer of the mammalian olfactory bulb. Brain Res. 1978 Dec 22;159(1):17–28. doi: 10.1016/0006-8993(78)90106-3. [DOI] [PubMed] [Google Scholar]
  5. Jahr C. E., Nicoll R. A. Dendrodendritic inhibition: demonstration with intracellular recording. Science. 1980 Mar 28;207(4438):1473–1475. doi: 10.1126/science.7361098. [DOI] [PubMed] [Google Scholar]
  6. Katz B., Miledi R. Tetrodotoxin-resistant electric activity in presynaptic terminals. J Physiol. 1969 Aug;203(2):459–487. doi: 10.1113/jphysiol.1969.sp008875. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Llinás R., Hess R. Tetrodotoxin-resistant dendritic spikes in avian Purkinje cells. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2520–2523. doi: 10.1073/pnas.73.7.2520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lux H. D., Pollen D. A. Electrical constants of neurons in the motor cortex of the cat. J Neurophysiol. 1966 Mar;29(2):207–220. doi: 10.1152/jn.1966.29.2.207. [DOI] [PubMed] [Google Scholar]
  9. Mori K., Nowycky M. C., Shepherd G. M. Analysis of a long-duration inhibitory potential in mitral cells in the isolated turtle olfactory bulb. J Physiol. 1981 May;314:311–320. doi: 10.1113/jphysiol.1981.sp013709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mori K., Nowycky M. C., Shepherd G. M. Analysis of synaptic potentials in mitral cells in the isolated turtle olfactory bulb. J Physiol. 1981 May;314:295–309. doi: 10.1113/jphysiol.1981.sp013708. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mori K., Satou M., Takagi S. F. Axonal projection of anterior olfactory nuclear neurons to the olfactory bulb bilaterally. Exp Neurol. 1979 May;64(2):295–305. doi: 10.1016/0014-4886(79)90270-x. [DOI] [PubMed] [Google Scholar]
  12. Mori K., Shepherd G. M. Synaptic excitation and long-lasting inhibition of mitral cells in the in vitro turtle olfactory bulb. Brain Res. 1979 Aug 17;172(1):155–159. doi: 10.1016/0006-8993(79)90904-1. [DOI] [PubMed] [Google Scholar]
  13. Mori K., Takagi S. F. An intracellular study of dendrodendritic inhibitory synapses on mitral cells in the rabbit olfactory bulb. J Physiol. 1978 Jun;279:569–588. doi: 10.1113/jphysiol.1978.sp012362. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mori K., Takagi S. F. Spike generation in the mitral cell dendrite of the rabbit olfactory bulb. Brain Res. 1975 Dec 26;100(3):685–689. doi: 10.1016/0006-8993(75)90170-5. [DOI] [PubMed] [Google Scholar]
  15. Nicoll R. A. Inhibitory mechanisms in the rabbit olfactory bulb: dendrodendritic mechanisms. Brain Res. 1969 Jun;14(1):157–172. doi: 10.1016/0006-8993(69)90037-7. [DOI] [PubMed] [Google Scholar]
  16. PHILLIPS C. G., POWELL T. P., SHEPHERD G. M. RESPONSES OF MITRAL CELLS TO STIMULATION OF THE LATERAL OLFACTORY TRACT IN THE RABBIT. J Physiol. 1963 Aug;168:65–88. doi: 10.1113/jphysiol.1963.sp007178. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Price J. L., Powell T. P. The synaptology of the granule cells of the olfactory bulb. J Cell Sci. 1970 Jul;7(1):125–155. doi: 10.1242/jcs.7.1.125. [DOI] [PubMed] [Google Scholar]
  18. Rall W., Shepherd G. M., Reese T. S., Brightman M. W. Dendrodendritic synaptic pathway for inhibition in the olfactory bulb. Exp Neurol. 1966 Jan;14(1):44–56. doi: 10.1016/0014-4886(66)90023-9. [DOI] [PubMed] [Google Scholar]
  19. Rall W., Shepherd G. M. Theoretical reconstruction of field potentials and dendrodendritic synaptic interactions in olfactory bulb. J Neurophysiol. 1968 Nov;31(6):884–915. doi: 10.1152/jn.1968.31.6.884. [DOI] [PubMed] [Google Scholar]
  20. Rall W. Time constants and electrotonic length of membrane cylinders and neurons. Biophys J. 1969 Dec;9(12):1483–1508. doi: 10.1016/S0006-3495(69)86467-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Reeves R. B. The interaction of body temperature and acid-base balance in ectothermic vertebrates. Annu Rev Physiol. 1977;39:559–586. doi: 10.1146/annurev.ph.39.030177.003015. [DOI] [PubMed] [Google Scholar]
  22. Schwartzkroin P. A. Further characteristics of hippocampal CA1 cells in vitro. Brain Res. 1977 Jun 3;128(1):53–68. doi: 10.1016/0006-8993(77)90235-9. [DOI] [PubMed] [Google Scholar]
  23. Schwartzkroin P. A., Prince D. A. Cellular and field potential properties of epileptogenic hippocampal slices. Brain Res. 1978 May 19;147(1):117–130. doi: 10.1016/0006-8993(78)90776-x. [DOI] [PubMed] [Google Scholar]
  24. Schwartzkroin P. A. Secondary range rhythmic spiking in hippocampal neurons. Brain Res. 1978 Jun 23;149(1):247–250. doi: 10.1016/0006-8993(78)90606-6. [DOI] [PubMed] [Google Scholar]
  25. Schwartzkroin P. A., Slawsky M. Probable calcium spikes in hippocampal neurons. Brain Res. 1977 Oct 21;135(1):157–161. doi: 10.1016/0006-8993(77)91060-5. [DOI] [PubMed] [Google Scholar]
  26. Shepherd G. M., Brayton R. K. Computer simulation of a dendrodendritic synaptic circuit for self- and lateral-inhibition in the olfactory bulb. Brain Res. 1979 Oct 19;175(2):377–382. doi: 10.1016/0006-8993(79)91020-5. [DOI] [PubMed] [Google Scholar]
  27. Shepherd G. M. Synaptic organization of the mammalian olfactory bulb. Physiol Rev. 1972 Oct;52(4):864–917. doi: 10.1152/physrev.1972.52.4.864. [DOI] [PubMed] [Google Scholar]
  28. Takahashi K. Slow and fast groups of pyramidal tract cells and their respective membrane properties. J Neurophysiol. 1965 Sep;28(5):908–924. doi: 10.1152/jn.1965.28.5.908. [DOI] [PubMed] [Google Scholar]
  29. Traub R. D., Llinás R. Hippocampal pyramidal cells: significance of dendritic ionic conductances for neuronal function and epileptogenesis. J Neurophysiol. 1979 Mar;42(2):476–496. doi: 10.1152/jn.1979.42.2.476. [DOI] [PubMed] [Google Scholar]
  30. Tsukahara N., Murakami F., Hultborn H. Electrical constants of neurons of the red nucleus. Exp Brain Res. 1975 Jul 11;23(1):49–64. doi: 10.1007/BF00238728. [DOI] [PubMed] [Google Scholar]
  31. Willey T. J. The ultrastructure of the cat olfactory bulb. J Comp Neurol. 1973 Dec 1;152(3):211–232. doi: 10.1002/cne.901520302. [DOI] [PubMed] [Google Scholar]
  32. YAMAMOTO C., YAMAMOTO T., IWAMA K. The inhibitory systems in the olfactory bulb studied by intracellular recording. J Neurophysiol. 1963 May;26:403–415. doi: 10.1152/jn.1963.26.3.403. [DOI] [PubMed] [Google Scholar]

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