Table 3.
Physicochemical properties and model quality statistics of the four proteins of mpox virus identified through subtractive proteomics.
| Features | Core protease I7 | DNA topoisomerase 1B | Early transcription factor 70 kDa small subunit | DNA polymerase processivity factor |
|---|---|---|---|---|
| Gene Name | I7L | TopIB | VETFS | A20R |
| Enzyme Commision (EC) | 3.2.22.- | 5.99.1.2 | 3.6.4.13 | 3.- |
| KEGG Orthology (KO) Identifier | – | – | – | K21082 |
| Closest Crystal Structure | – | 2H7G (97.77%, 100%)) | 7AMV (99.69%, 100%) | 6ZXP (96.75%, 28%) |
| Length (Amino Acid) | 423 aa | 314 aa | 637 aa | 426 aa |
| Mol. Weight (Dalton) | 49023.57 | 36665.56 | 73844.91 | 49147.14 |
| pI | 7.85 | 9.50 | 6.93 | 5.62 |
| Instability Index | 33.81 | 40.22 | 34.45 | 36.35 |
| Aliphatic Index | 83.14 | 88.03 | 97.85 | 92.77 |
| GRAVY | −0.22 | −0.32 | −0.17 | −0.26 |
| Predicted IDDT Score | 0.64 | 0.87 | 0.70 | 0.80 |
| Overall Quality Score | 95.42 | 95.75 | 95.66 | 91.87 |
| MolProbity Score | 1.44 (96th percentile) | 0.90 (100th percentile) | 1.40 (97th percentile) | 1.37 (98th percentile) |
| Clash Score | 3.49 (97th percentile) | 1.53 (99th percentile) | 2.78 (98th percentile) | 2.88 (98th percentile) |
| Poor Rotamers (%) | 0 | 0 | 0 | 0 |
| RamaPlot Most Favoured (%) | 95.72 (403 aa) | 100.00 (312 aa) | 95.12 (604 aa) | 95.80 (406 aa) |
| RamaPlot Allowed (%) | 3.8 (16 aa) | 0 | 3.48 (22 aa) | 4 (17 aa) |
| RamaPlot Disallowed (%) | 0.48 (2 aa) | 0 | 1.42 (9 aa) | 0.24 (1 aa) |