Fig. 5.
PAA:Nanoalum creates an immunocompetent lymph node environment and activates the inflammasome. a The amount of IL-18, IL-12p70 and IFN-γ in the draining lymph node 6 h after immunization with saline, Alhydrogel or PAA:nanoalum. b At the same time, the numbers of neutrophils and subcapsular macrophages were enumerated and intracellular IL-1β and IFN-γ expression were determined by ICS. c Antigen-specific CD4 T cells were determined in wild-type (WT), ASC-/-, NLRP3-/-, or IL-18R-/- mice 1 week after the boosting immunization with either antigen alone or antigen adjuvanted with PAA:nanoalum using ICS. Representative FACS gatings are shown in Supplementary Fig. 3A, B. N = 5 mice per group. The data are representative of two experiments with similar results. Bars are drawn to the means with whiskers to the standard deviations